Development of bacterial oxidative stress assays
... compared to traditional methods of assessing bacterial viability including spread plating and measuring growth by optical density. The concentration of E. coli cells and the type of 96-well plate used in the fluorescence microplate reader affected the ability of the fluorescence assay to accurately ...
... compared to traditional methods of assessing bacterial viability including spread plating and measuring growth by optical density. The concentration of E. coli cells and the type of 96-well plate used in the fluorescence microplate reader affected the ability of the fluorescence assay to accurately ...
Unicellular Organisms
... All of the Organisms we talk about today will be eukaryotic. They are protists: eukaryotes that cannot be classified as animals, plants, or fungi. ...
... All of the Organisms we talk about today will be eukaryotic. They are protists: eukaryotes that cannot be classified as animals, plants, or fungi. ...
Regulation of endothelial and hematopoietic development by the
... requirement for Etv2 during early hematopoiesis in mice, only the myeloid lineage is affected in zebrafish Etv2 mutants, whereas erythroid lineage development occurs normally [22,25,26,30 ,31]. However, Ren et al. [21] have shown that Etv2 is also required for the initiation of hematopoietic stem ce ...
... requirement for Etv2 during early hematopoiesis in mice, only the myeloid lineage is affected in zebrafish Etv2 mutants, whereas erythroid lineage development occurs normally [22,25,26,30 ,31]. However, Ren et al. [21] have shown that Etv2 is also required for the initiation of hematopoietic stem ce ...
Understanding Activity in Electrically Coupled Networks Using PRCs
... Iapp > Iionic .Vj / for all Vreset Vj Vth , then Vj increases monotonically from Vreset until it reaches the threshold potential Vth at which point the cell “fires.” Vj is then reset to Vreset , and the process is repeated. Thus, for sufficiently large applied current, integrate-and-fire cells d ...
... Iapp > Iionic .Vj / for all Vreset Vj Vth , then Vj increases monotonically from Vreset until it reaches the threshold potential Vth at which point the cell “fires.” Vj is then reset to Vreset , and the process is repeated. Thus, for sufficiently large applied current, integrate-and-fire cells d ...
Uptake of glutamate, not glutamine synthetase, regulates adaptation
... 1962). Cell culture media in general usually contain relatively high concentrations of glutamine (2-4 mM). This derives from the original media formulations which were based upon observed cellular requirements (Eagle, 1955, 1959). Glutamine is required as a substrate and precursor for protein synthe ...
... 1962). Cell culture media in general usually contain relatively high concentrations of glutamine (2-4 mM). This derives from the original media formulations which were based upon observed cellular requirements (Eagle, 1955, 1959). Glutamine is required as a substrate and precursor for protein synthe ...
Cell Lineage and Fate Map of the Primary
... embryonic cells can be readily identified among distantly related animals. Spiralian embryos are particularly amenable to studies of fate-mapping, and larval fates of identified cells are conserved among diverse taxa. One cell of particular importance in spiralian development is 2d, or the primary s ...
... embryonic cells can be readily identified among distantly related animals. Spiralian embryos are particularly amenable to studies of fate-mapping, and larval fates of identified cells are conserved among diverse taxa. One cell of particular importance in spiralian development is 2d, or the primary s ...
In Vivo Release of Mitotic Silencing of Ribosomal Gene
... pol I, upstream binding factor [UBF], TATA-binding protein [TBP]-containing factor SL1) that are sufficient to promote rDNA transcription in vitro (for review see Moss ...
... pol I, upstream binding factor [UBF], TATA-binding protein [TBP]-containing factor SL1) that are sufficient to promote rDNA transcription in vitro (for review see Moss ...
The Expanding Role of the mammalian Target of Rapamycin
... stress response, are both required during cell proliferation and survival under stress. To maintain homeostasis, GSK-3β has been shown to negatively regulate mTORC2 by phosphorylating rictor at S1235 [73]. This S1235 inhibitory phosphorylation impairs the ability of mTORC2 to phosphorylate and activ ...
... stress response, are both required during cell proliferation and survival under stress. To maintain homeostasis, GSK-3β has been shown to negatively regulate mTORC2 by phosphorylating rictor at S1235 [73]. This S1235 inhibitory phosphorylation impairs the ability of mTORC2 to phosphorylate and activ ...
1 - AState.edu
... • Within the nucleus, long DNA molecules and associated proteins form fibers called chromatin. • Each long chromatin fiber constitutes one chromosome. • The nucleolus is • a prominent structure within the nucleus and • the site where the components of ribosomes are made. ...
... • Within the nucleus, long DNA molecules and associated proteins form fibers called chromatin. • Each long chromatin fiber constitutes one chromosome. • The nucleolus is • a prominent structure within the nucleus and • the site where the components of ribosomes are made. ...
Decreased argyrophilic nucleolar organiser region
... score’ of the AgNOR expression did not differ from that of agematched controls. However, a noteworthy finding was related to a ‘specific score’ detected in the cerebellar cortex of nine SIUDS and four SIDS cases. Surprisingly, in fact, intermixed with several PCs showing a swollen, shrunken morphology ...
... score’ of the AgNOR expression did not differ from that of agematched controls. However, a noteworthy finding was related to a ‘specific score’ detected in the cerebellar cortex of nine SIUDS and four SIDS cases. Surprisingly, in fact, intermixed with several PCs showing a swollen, shrunken morphology ...
Discs large 5, an Essential Gene in Drosophila, Regulates Egg
... the idea that loss of dlg5 affects cell division or cell viability. From these results we can conclude that Dlg5 is essential in both germline and somatic stem cells, and it is likely required in all follicle cells throughout oogenesis. Reduced levels of Dlg5 in follicle cells leads to egg chamber d ...
... the idea that loss of dlg5 affects cell division or cell viability. From these results we can conclude that Dlg5 is essential in both germline and somatic stem cells, and it is likely required in all follicle cells throughout oogenesis. Reduced levels of Dlg5 in follicle cells leads to egg chamber d ...
PDF
... simulated microgravity conditions. The aim was to correlate these parameters with the ability of the bacteria to attach and form biofilm. Micrococcus luteus is a Gram-positive, nonmotile, spherical, saprotrophic bacterium that belongs to the family of the Micrococcaceae. The obligate aerobe M. luteu ...
... simulated microgravity conditions. The aim was to correlate these parameters with the ability of the bacteria to attach and form biofilm. Micrococcus luteus is a Gram-positive, nonmotile, spherical, saprotrophic bacterium that belongs to the family of the Micrococcaceae. The obligate aerobe M. luteu ...
PDF only - at www.arxiv.org.
... 1.1 The in vitro study of the effect of topography on nerve cells The role of soluble (bio)chemical signals in cell shape, cell adhesion, differentiation and axon guidance, is well established [1]. In addition to the biochemical signals, there is increasing evidence that the physical parameters (e.g ...
... 1.1 The in vitro study of the effect of topography on nerve cells The role of soluble (bio)chemical signals in cell shape, cell adhesion, differentiation and axon guidance, is well established [1]. In addition to the biochemical signals, there is increasing evidence that the physical parameters (e.g ...
Patterns of pathogenesis: discrimination of pathogenic and nonpathogenic microbes by the innate immune system.
... pathogen sensing may complement PAMP sensing. These additional mechanisms might, for example, permit specific recognition of microorganisms that have high pathogenic potential. The barcode hypothesis is one model that has been proposed to explain how responses could be tailor-made to certain microbe ...
... pathogen sensing may complement PAMP sensing. These additional mechanisms might, for example, permit specific recognition of microorganisms that have high pathogenic potential. The barcode hypothesis is one model that has been proposed to explain how responses could be tailor-made to certain microbe ...
ARVO 2017 Annual Meeting Abstracts 106 Development of the
... Results: We investigated the roles of factors that execute or regulate Yki function. As Yki is known to promote cell proliferation and survival in Metazoa, we probed whether these functions of Yki are concomitant or distinct from its role in cell fate. We found that exogenous expression of the cell ...
... Results: We investigated the roles of factors that execute or regulate Yki function. As Yki is known to promote cell proliferation and survival in Metazoa, we probed whether these functions of Yki are concomitant or distinct from its role in cell fate. We found that exogenous expression of the cell ...
Shaping the metaphase chromosome: coordination of cohesion and
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
Gastrulation: Making and Shaping Germ Layers
... of cell fates along the embryonic axes. These processes occur very early during development when most embryos consist of a relatively small number of morphologically similar cells arranged in simple structures, such as cell balls or sheets, which can be flat or cup shaped. The term gastrulation, deri ...
... of cell fates along the embryonic axes. These processes occur very early during development when most embryos consist of a relatively small number of morphologically similar cells arranged in simple structures, such as cell balls or sheets, which can be flat or cup shaped. The term gastrulation, deri ...
Three-Dimensional Organization of Drosophila melanogaster
... changes. If they occur asynchronously within a tissue, such changes could account for the finding that chromosome configurations have only limited similarities between cells of the salivary gland (28). Different cells within the same gland are known to differ in the timing of glue granule formation ...
... changes. If they occur asynchronously within a tissue, such changes could account for the finding that chromosome configurations have only limited similarities between cells of the salivary gland (28). Different cells within the same gland are known to differ in the timing of glue granule formation ...
Evidence that Shh cooperates with a retinoic acid inducible co
... To test for polarizing activity and to investigate the regulation of Shh expression in the limb tissue, grafts were made under a flap of apical ridge, lifted along the anterior margin of wing buds at stages 20-21. The embryos were then reincubated at 38°C for a further 4-30 hours. After incubation t ...
... To test for polarizing activity and to investigate the regulation of Shh expression in the limb tissue, grafts were made under a flap of apical ridge, lifted along the anterior margin of wing buds at stages 20-21. The embryos were then reincubated at 38°C for a further 4-30 hours. After incubation t ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.