The Role of Lysosomes in Molluscan Inflammation1
... Marine Biomedical Research Program and Department of Anatomy (Cell Biology), Medical University of South Carolina, Charleston, South Carolina 29412 ...
... Marine Biomedical Research Program and Department of Anatomy (Cell Biology), Medical University of South Carolina, Charleston, South Carolina 29412 ...
Chara Myosin and the Energy of Cytoplasmic Streaming
... 10 mg ml1 bovine serum albumin dissolved in phosphatebuffered saline using an Ostwald viscometer at 208C. The relative density r of solutions was determined by weighing the same volume of solutions sucked with the same liquid dispenser. Flow time t and relative density are shown in Table 1. The vis ...
... 10 mg ml1 bovine serum albumin dissolved in phosphatebuffered saline using an Ostwald viscometer at 208C. The relative density r of solutions was determined by weighing the same volume of solutions sucked with the same liquid dispenser. Flow time t and relative density are shown in Table 1. The vis ...
regulation of secretion of parathormone and secretory protein
... to allow us to discern simultaneously the fate of radioactive parathormone made by the cell before and coincident with stimulation by dibutyryl cyclic AMP. Table II shows that the amount of total parathormone secreted was 3.5- to 4-fold greater at 0.5 mM calcium than at 3 .0 mM calcium. At both conc ...
... to allow us to discern simultaneously the fate of radioactive parathormone made by the cell before and coincident with stimulation by dibutyryl cyclic AMP. Table II shows that the amount of total parathormone secreted was 3.5- to 4-fold greater at 0.5 mM calcium than at 3 .0 mM calcium. At both conc ...
Exosome Biogenesis, Regulation, and Function in Viral Infection
... are also used for intercellular communication, to receive and send signals. These have different sizes, distinct origins, and are formed using a multitude of mechanisms. It has long been known that apoptotic cells shed large vesicles of about 500–2000 nm in diameter that can be taken up by phagocyti ...
... are also used for intercellular communication, to receive and send signals. These have different sizes, distinct origins, and are formed using a multitude of mechanisms. It has long been known that apoptotic cells shed large vesicles of about 500–2000 nm in diameter that can be taken up by phagocyti ...
Robust mechanisms of ventral furrow invagination require the
... contribute to the physical process of ventral furrow formation, we discretize a two-dimensional section of the embryo by resorting to finite elements methods (FEM). In recent years, this type of approach has been successfully applied to multicellular animal morphogenesis (Clausi and Brodland 1993, B ...
... contribute to the physical process of ventral furrow formation, we discretize a two-dimensional section of the embryo by resorting to finite elements methods (FEM). In recent years, this type of approach has been successfully applied to multicellular animal morphogenesis (Clausi and Brodland 1993, B ...
Functional Analysis of Cellulose and Xyloglucan
... seedlings were used in this experiment because preliminary analyses demonstrated that there is a dramatic reduction in fluorescent protein (FP)-CESA1/3/6 particle density mm22 in guard cells from 1 to 2 weeks after germination (Supplemental Fig. S1). To validate that stomatal guard cells from young s ...
... seedlings were used in this experiment because preliminary analyses demonstrated that there is a dramatic reduction in fluorescent protein (FP)-CESA1/3/6 particle density mm22 in guard cells from 1 to 2 weeks after germination (Supplemental Fig. S1). To validate that stomatal guard cells from young s ...
Innexin7a forms junctions that stabilize the basal
... The formation of the furrow canals during the preliminary phase is a conspicuous start to cellularization in Drosophila. The furrow canals are the dilated leading edges of the ingressing membrane to which actin is recruited so that an apical interconnected hexagonal actin network is formed around ea ...
... The formation of the furrow canals during the preliminary phase is a conspicuous start to cellularization in Drosophila. The furrow canals are the dilated leading edges of the ingressing membrane to which actin is recruited so that an apical interconnected hexagonal actin network is formed around ea ...
- Wiley Online Library
... Several studies have implicated Zfp281/ZNF281 in regulating stem cell pluripotency and developmental processes. First, an earlier study [17] using transcriptomic profiling of human ESCs and their differentiated progenies identified ZNF281 as one of the upregulated transcriptional regulators in undiffe ...
... Several studies have implicated Zfp281/ZNF281 in regulating stem cell pluripotency and developmental processes. First, an earlier study [17] using transcriptomic profiling of human ESCs and their differentiated progenies identified ZNF281 as one of the upregulated transcriptional regulators in undiffe ...
Insulin-Like Growth Factor and Potassium Depolarization Maintain
... increased number of fluorescent (viable) cells in elevated K1 and IGF-1 treated cultures (a, c) compared with culture switched to low K1 (b). For viability assays, an 0.85 mm2 field is randomly chosen from the culture dish and acquired using the NIH Image program. Stained cells in each field are the ...
... increased number of fluorescent (viable) cells in elevated K1 and IGF-1 treated cultures (a, c) compared with culture switched to low K1 (b). For viability assays, an 0.85 mm2 field is randomly chosen from the culture dish and acquired using the NIH Image program. Stained cells in each field are the ...
Tissue Engineered Human Skin Equivalents
... process and up-regulated the genes necessary for wound healing [13]. Another approach to stimulate ...
... process and up-regulated the genes necessary for wound healing [13]. Another approach to stimulate ...
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in
... The functional consequences of Connexin43 (Cx43) phosphorylation remain largely unexplored. Using an antibody that specifically recognizes Cx43 phosphorylated at serine residues 325, 328 and/or 330 (pS325/328/330Cx43), we show that labeling of this form of Cx43 as well as of total Cx43 is restricted ...
... The functional consequences of Connexin43 (Cx43) phosphorylation remain largely unexplored. Using an antibody that specifically recognizes Cx43 phosphorylated at serine residues 325, 328 and/or 330 (pS325/328/330Cx43), we show that labeling of this form of Cx43 as well as of total Cx43 is restricted ...
Plant Growth Regulators II: Cytokinins, their Analogues and
... A common way of adding a natural cytokinin to media is by the use of organic supplements such as yeast extract or coconut milk. Coconut milk contains several physiologically-active substances (see Chapter 4); amongst which have been found the natural cytokinin zeatin, and 1,3-diphenylurea (11) (Shan ...
... A common way of adding a natural cytokinin to media is by the use of organic supplements such as yeast extract or coconut milk. Coconut milk contains several physiologically-active substances (see Chapter 4); amongst which have been found the natural cytokinin zeatin, and 1,3-diphenylurea (11) (Shan ...
Cell adhesion and cell mechanics during zebrafish
... and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression among the emergent cell types [2]. At the end, however, it is not sufficient t ...
... and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression among the emergent cell types [2]. At the end, however, it is not sufficient t ...
Here - Muscle Health Research Centre
... media and incubating newly isolated single fibers for 24 h. After this duration, fibers were briefly fixed in 4% paraformaldehyde (PFA), incubated in 2 N HCl for 1 h at 37°C, followed by blocking buffer (1.5% normal goat serum, 1.5% normal horse serum) for 1 h. BrdU antibody (Sigma B2531) was dilute ...
... media and incubating newly isolated single fibers for 24 h. After this duration, fibers were briefly fixed in 4% paraformaldehyde (PFA), incubated in 2 N HCl for 1 h at 37°C, followed by blocking buffer (1.5% normal goat serum, 1.5% normal horse serum) for 1 h. BrdU antibody (Sigma B2531) was dilute ...
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... reflect a failure in boundary capture or some similar behavior at the notochord border. Wild-type ascidian embryos exhibit a suite of complex behaviors at the notochord border, including a surprisingly dynamic boundary capture-like behavior in which notochord cells rapidly spread out on surfaces con ...
... reflect a failure in boundary capture or some similar behavior at the notochord border. Wild-type ascidian embryos exhibit a suite of complex behaviors at the notochord border, including a surprisingly dynamic boundary capture-like behavior in which notochord cells rapidly spread out on surfaces con ...
The transplantation of nuclei from single cultured cells into
... of genes in the nuclei of cultured cells. We have obtained one first-transfer and two serial-transfer embryos which have passed successfully through metamorphosis (Fig. 3D). Until the age of sexual maturity, their capacity for gametogenesis is not known, but these frogs appear to contain normal cell ...
... of genes in the nuclei of cultured cells. We have obtained one first-transfer and two serial-transfer embryos which have passed successfully through metamorphosis (Fig. 3D). Until the age of sexual maturity, their capacity for gametogenesis is not known, but these frogs appear to contain normal cell ...
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... of genes in the nuclei of cultured cells. We have obtained one first-transfer and two serial-transfer embryos which have passed successfully through metamorphosis (Fig. 3D). Until the age of sexual maturity, their capacity for gametogenesis is not known, but these frogs appear to contain normal cell ...
... of genes in the nuclei of cultured cells. We have obtained one first-transfer and two serial-transfer embryos which have passed successfully through metamorphosis (Fig. 3D). Until the age of sexual maturity, their capacity for gametogenesis is not known, but these frogs appear to contain normal cell ...
Actin Interacting Protein1 and Actin Depolymerizing
... The remodeling of actin networks is required for a variety of cellular processes in eukaryotes. In plants, several actin binding proteins have been implicated in remodeling cortical actin filaments (F-actin). However, the extent to which these proteins support F-actin dynamics in planta has not been ...
... The remodeling of actin networks is required for a variety of cellular processes in eukaryotes. In plants, several actin binding proteins have been implicated in remodeling cortical actin filaments (F-actin). However, the extent to which these proteins support F-actin dynamics in planta has not been ...
Ultrastructural immunocytochemical localization of
... intensity from that in the gastrulating embryo. Formation of the basal lamina during this developmental period has been followed in some detail (Low, 1967; Sanders, 1979) and the gradual morphological elaboration described. It would now be of interest to examine the basal lamina before the time of l ...
... intensity from that in the gastrulating embryo. Formation of the basal lamina during this developmental period has been followed in some detail (Low, 1967; Sanders, 1979) and the gradual morphological elaboration described. It would now be of interest to examine the basal lamina before the time of l ...
At the Intersection of the Pathways for Exocytosis and
... starvation adaption, energy balance, organelle clearance, immunity and cell death. In human diseases, such as cancers, neurodegenerative disorders (e.g. Huntington’s disease), and chronic inflammatory diseases (e.g. Crohn’s disease), there have been reports of functional disparity in both of these i ...
... starvation adaption, energy balance, organelle clearance, immunity and cell death. In human diseases, such as cancers, neurodegenerative disorders (e.g. Huntington’s disease), and chronic inflammatory diseases (e.g. Crohn’s disease), there have been reports of functional disparity in both of these i ...
Somite dorsoventral polarity - Development
... and Asamoto rotated whole, intact somites while, in the challenge experiments noted above, only sclerotome fragments were analyzed. It is not difficult to imagine differences in behavior of whole somites and somite fragments. In order to minimize such differences we repeated the somite rotation expe ...
... and Asamoto rotated whole, intact somites while, in the challenge experiments noted above, only sclerotome fragments were analyzed. It is not difficult to imagine differences in behavior of whole somites and somite fragments. In order to minimize such differences we repeated the somite rotation expe ...
Thesis titel 1.5 final - SciDok
... isolation of embryonic stem cells, discovery of adult stem cells and their multi-lineage differentiation raise new hopes in the field of medicine for using these cells in regenerative and transplantation therapy. However, even using the state of the art techniques, it is not possible yet to culture ...
... isolation of embryonic stem cells, discovery of adult stem cells and their multi-lineage differentiation raise new hopes in the field of medicine for using these cells in regenerative and transplantation therapy. However, even using the state of the art techniques, it is not possible yet to culture ...
Cell-cycle-regulated expression of STIL controls centriole number in
... separate and contribute to the formation of the bipolar mitotic spindle. Association with the spindle ensures the equal distribution of centrioles to nascent daughter cells. Late in mitosis, the tight connection between mother and daughter centriole is severed in a process termed centriole disengage ...
... separate and contribute to the formation of the bipolar mitotic spindle. Association with the spindle ensures the equal distribution of centrioles to nascent daughter cells. Late in mitosis, the tight connection between mother and daughter centriole is severed in a process termed centriole disengage ...
a gene required for dorsoventrality of leaves in
... with roles in protecting the developing flower or attracting pollinators, but it is also apparent in the pollen sacs of stamens and the the ovary wall of the gynoecium (Goebel, 1905). The mechanisms that define dorsoventrality may therefore be similar in all lateral organs of the plant. At the devel ...
... with roles in protecting the developing flower or attracting pollinators, but it is also apparent in the pollen sacs of stamens and the the ovary wall of the gynoecium (Goebel, 1905). The mechanisms that define dorsoventrality may therefore be similar in all lateral organs of the plant. At the devel ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.