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Cells Producing - The Journal of Immunology
... the Thy1a (Thy1.1) allele and can be recognized easily by flow cytometric analysis using a Thy1.1-specific Ab. Naive Tconv and Treg populations used in this study were confirmed by assessing Foxp3 expression (data not shown). Culture of Tconvs with antiCD3 mAb and APCs did not induce IL-17–producing ...
... the Thy1a (Thy1.1) allele and can be recognized easily by flow cytometric analysis using a Thy1.1-specific Ab. Naive Tconv and Treg populations used in this study were confirmed by assessing Foxp3 expression (data not shown). Culture of Tconvs with antiCD3 mAb and APCs did not induce IL-17–producing ...
Starvation Induces Vacuolar Targeting and Degradation of the
... and resuspended at an OD600 5 0.5 in prewarmed (378C) SC medium containing cold methionine and rapamycin (200 ng/ml). Culture aliquots equal to 15 OD600 of cells were removed after 0, 30, 60, and 90 min, and kept on ice for 10 min in the presence of 10 mM NaN3 and 10 mM NaF. Cells were harvested and ...
... and resuspended at an OD600 5 0.5 in prewarmed (378C) SC medium containing cold methionine and rapamycin (200 ng/ml). Culture aliquots equal to 15 OD600 of cells were removed after 0, 30, 60, and 90 min, and kept on ice for 10 min in the presence of 10 mM NaN3 and 10 mM NaF. Cells were harvested and ...
Filamentous Influenza Virus Enters Cells via
... engulfed, intact, from the cell surface and traffic to an acidified compartment within the cell in which the low-pH environment allows for M2-mediated alterations in membrane curvature, fragmenting the filamentous virions and allowing for membrane fusion and the release of the viral genome. MATERIAL ...
... engulfed, intact, from the cell surface and traffic to an acidified compartment within the cell in which the low-pH environment allows for M2-mediated alterations in membrane curvature, fragmenting the filamentous virions and allowing for membrane fusion and the release of the viral genome. MATERIAL ...
Emergent Synchronous Bursting of Oxytocin Neuronal Network
... are generated in the cell bodies and which are propagated down their axons to the nerve endings. Normally, oxytocin cells discharge asynchronously at 1–3 spikes/s, but during suckling, every 5 min or so, each discharges a brief, intense burst of spikes that release a pulse of oxytocin into the circu ...
... are generated in the cell bodies and which are propagated down their axons to the nerve endings. Normally, oxytocin cells discharge asynchronously at 1–3 spikes/s, but during suckling, every 5 min or so, each discharges a brief, intense burst of spikes that release a pulse of oxytocin into the circu ...
Centrosome Maturation and Mitotic Spindle Assembly in C. elegans
... spd-5 Mutants Fail to Organize Microtubules into a Bipolar Mitotic Spindle We used indirect immunofluorescence and confocal microscopy to compare the organization of microtubules and chromosomes in fixed wild-type and spd-5 mutant embryos (Figure 2A). In wild-type, short cytoplasmic MTs were dispers ...
... spd-5 Mutants Fail to Organize Microtubules into a Bipolar Mitotic Spindle We used indirect immunofluorescence and confocal microscopy to compare the organization of microtubules and chromosomes in fixed wild-type and spd-5 mutant embryos (Figure 2A). In wild-type, short cytoplasmic MTs were dispers ...
Vesicle trafficking dynamics and visualization of zones of exocytosis
... proteins that are recycled by trafficking to the enodosomal compartment, as well as bulk fluid and soluble molecules (pinocytosis). Endocytic vesicles can invaginate into coated pits and pinch-off as clathrin-coated vesicles (Maldonado-Baez and Wendland, 2006). Electron microscopy on cryo-fixed seri ...
... proteins that are recycled by trafficking to the enodosomal compartment, as well as bulk fluid and soluble molecules (pinocytosis). Endocytic vesicles can invaginate into coated pits and pinch-off as clathrin-coated vesicles (Maldonado-Baez and Wendland, 2006). Electron microscopy on cryo-fixed seri ...
The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to
... both were able to precipitate rhodopsin and Arf4–GppNHp. A specific complex with rhodopsin was formed when Arf4– GppNHp, ASAP1 and Rab11a were present (Fig. 2E), as determined in previous studies (Wang et al., 2012). When FIP3 ...
... both were able to precipitate rhodopsin and Arf4–GppNHp. A specific complex with rhodopsin was formed when Arf4– GppNHp, ASAP1 and Rab11a were present (Fig. 2E), as determined in previous studies (Wang et al., 2012). When FIP3 ...
Pathogenesis of respiratory infections caused by Mycoplasma dispar
... substances present in the secretions that line the airways and alveolar walls (Sleigh et al., 1988). These secretions contain components such as surfactant, bacteriostatic iron-containing compounds, complement fractions, and gamma globulin (Roitt, 1988). Cellular factors involved in the defense of t ...
... substances present in the secretions that line the airways and alveolar walls (Sleigh et al., 1988). These secretions contain components such as surfactant, bacteriostatic iron-containing compounds, complement fractions, and gamma globulin (Roitt, 1988). Cellular factors involved in the defense of t ...
Agglutination
... • S.aureus: most frequently used because it has protein A in its outer surface that naturally adsorbs the Fc portion of the antibody ...
... • S.aureus: most frequently used because it has protein A in its outer surface that naturally adsorbs the Fc portion of the antibody ...
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... Medicinal and aromatic plants are widely used as medicine and constitute a major source of natural organic compounds (Seenivasan et al., 2006). Research data has demonstrated that the extracts exhibit various biological effects, such as antibacterial, antihyperglycemic and antioxidant activities (Le ...
... Medicinal and aromatic plants are widely used as medicine and constitute a major source of natural organic compounds (Seenivasan et al., 2006). Research data has demonstrated that the extracts exhibit various biological effects, such as antibacterial, antihyperglycemic and antioxidant activities (Le ...
Millius et al., J. Cell Sci., 2012 - Cardiovascular Research Institute
... Fig. 2. Actin and p40 retrograde flow is similar to WAVE2 retrograde flow. (A) Left panel shows epifluorescence image of an XTC cell expressing a low concentration of EGFP-tagged p40 subunit of the Arp2/3 complex (corresponds to supplementary material Movie 3). Scale bar: 5 mm. Middle panel shows ma ...
... Fig. 2. Actin and p40 retrograde flow is similar to WAVE2 retrograde flow. (A) Left panel shows epifluorescence image of an XTC cell expressing a low concentration of EGFP-tagged p40 subunit of the Arp2/3 complex (corresponds to supplementary material Movie 3). Scale bar: 5 mm. Middle panel shows ma ...
Multiple mechanisms determine the order of APC/C substrate
... Sullivan and Morgan, 2007). It is not clear how the same APC/C complex robustly distinguishes among its substrates and promotes their degradation at different times in the cell cycle. The timing of APC/CCdc20 substrate degradation in vertebrate cells is influenced by the spindle assembly checkpoint ...
... Sullivan and Morgan, 2007). It is not clear how the same APC/C complex robustly distinguishes among its substrates and promotes their degradation at different times in the cell cycle. The timing of APC/CCdc20 substrate degradation in vertebrate cells is influenced by the spindle assembly checkpoint ...
ATR Regulates a G2-Phase Cell-Cycle Checkpoint
... mutants, resulting in compaction of nuclei and subsequent cell death. By contrast, HU-treated wild-type and atr plants arrested in G1 and showed no obvious signs of cell death. We propose that, in plants, HU invokes a novel checkpoint responsive to low levels of deoxynucleotide triphosphates. These ...
... mutants, resulting in compaction of nuclei and subsequent cell death. By contrast, HU-treated wild-type and atr plants arrested in G1 and showed no obvious signs of cell death. We propose that, in plants, HU invokes a novel checkpoint responsive to low levels of deoxynucleotide triphosphates. These ...
Cytokinesis defines a spatial landmark for hepatocyte polarization
... In this study, using the rat hepatocyte cell line Can10 that polarizes and forms bile canaliculi robustly in vitro (Treyer and Müsch, 2013; Decaens et al., 2008; Peng et al., 2006), we find that hepatocyte polarization and apical lumen formation are linked to cytokinesis. In addition, we find that ...
... In this study, using the rat hepatocyte cell line Can10 that polarizes and forms bile canaliculi robustly in vitro (Treyer and Müsch, 2013; Decaens et al., 2008; Peng et al., 2006), we find that hepatocyte polarization and apical lumen formation are linked to cytokinesis. In addition, we find that ...
05 October 2000
... Biology Department, University of Montreal, Montreal , Quebec H1X 2B2, Canada Correspondence and requests for materials should be addressed to M.C. (e-mail: [email protected]). ...
... Biology Department, University of Montreal, Montreal , Quebec H1X 2B2, Canada Correspondence and requests for materials should be addressed to M.C. (e-mail: [email protected]). ...
Temporal and Spatial Distribution of DNA Topoisomerase II Alters
... from late S to G2/M phases (Fig 1B). In G1 phase, topo IIa content varies from almost zero to somewhat larger than that of early S phase. On the contrary, topo IIb level slightly increases in G1 phase and thereafter is not significantly altered through the cell cycle (Fig 1C). Although the topo IIb ...
... from late S to G2/M phases (Fig 1B). In G1 phase, topo IIa content varies from almost zero to somewhat larger than that of early S phase. On the contrary, topo IIb level slightly increases in G1 phase and thereafter is not significantly altered through the cell cycle (Fig 1C). Although the topo IIb ...
Mitochondria in Lung Biology and Pathology: More than - AJP-Lung
... attendees, too, recalled this as their first introduction to mitochondria and often their first ...
... attendees, too, recalled this as their first introduction to mitochondria and often their first ...
The construction of an enhanced green fluorescence protein
... vectors were linearized by cutting with restriction enzymes. pCDNA3 was linerized using BamHI and EcoRI whereas pDRIVE-mMb and pDRIVE-mDesmin were linearized using NcoI and EcoRI. Vector backbones were isolated by resolving on 1% agarose gels followed by purifcation using so and so’s gel purifcation ...
... vectors were linearized by cutting with restriction enzymes. pCDNA3 was linerized using BamHI and EcoRI whereas pDRIVE-mMb and pDRIVE-mDesmin were linearized using NcoI and EcoRI. Vector backbones were isolated by resolving on 1% agarose gels followed by purifcation using so and so’s gel purifcation ...
Protists Cells: Grow and Develop:
... Protists and the animal and plant kingdoms, they still use these kingdoms to help describe the different types of Protists. Protists are animal like, plant like, or fungi like. Protists are so small that they do not need any special organelles to take in gas or get rid of waste. They rely on simple ...
... Protists and the animal and plant kingdoms, they still use these kingdoms to help describe the different types of Protists. Protists are animal like, plant like, or fungi like. Protists are so small that they do not need any special organelles to take in gas or get rid of waste. They rely on simple ...
Bacterial chromosome segregation
... to opposite halves of the cell. The G1 phase is the period from the end of cell division to the beginning of the S phase. The eukaryotic cell-cycle nomenclature has been applied to bacteria with some modifications (Helmstetter, 1996). The DNA synthesis phase (S) corresponds to the C period in bacter ...
... to opposite halves of the cell. The G1 phase is the period from the end of cell division to the beginning of the S phase. The eukaryotic cell-cycle nomenclature has been applied to bacteria with some modifications (Helmstetter, 1996). The DNA synthesis phase (S) corresponds to the C period in bacter ...
The ultrastructure of chilling stress
... chilling stress is no exception. There are many factors such as light intensity, relative humidity and the inherent sensitivity of the plant to chilling that interact and may either enhance the effect or act as protection against injury. Indeed, it is arguably next to impossible to control for all o ...
... chilling stress is no exception. There are many factors such as light intensity, relative humidity and the inherent sensitivity of the plant to chilling that interact and may either enhance the effect or act as protection against injury. Indeed, it is arguably next to impossible to control for all o ...
Kratsch, H.A . and R.R. Wise. 2000. The ultrastructure of chilling stress. Plant, Cell Environment 23: 337-350.
... chilling stress is no exception. There are many factors such as light intensity, relative humidity and the inherent sensitivity of the plant to chilling that interact and may either enhance the effect or act as protection against injury. Indeed, it is arguably next to impossible to control for all o ...
... chilling stress is no exception. There are many factors such as light intensity, relative humidity and the inherent sensitivity of the plant to chilling that interact and may either enhance the effect or act as protection against injury. Indeed, it is arguably next to impossible to control for all o ...
- ORCA - Cardiff University
... modulation of the expression levels or the activity of components of this apoptotic balance changes the sensitivity to deathpromoting cues, allowing temporal restriction of cell death. Programmed cell death eliminates many neurons during development, even in organisms comprised of only few cells, su ...
... modulation of the expression levels or the activity of components of this apoptotic balance changes the sensitivity to deathpromoting cues, allowing temporal restriction of cell death. Programmed cell death eliminates many neurons during development, even in organisms comprised of only few cells, su ...
The Crabtree Effect: A Review
... respiration employed phosphate buffers. Theoretically, these differences in findings could be attributed to the fact that the glycolytic inhibitors prevent the accumulation of H +, which also inhibits respiration. However, the data of the workers who find a release of the Crabtree effect by an inhib ...
... respiration employed phosphate buffers. Theoretically, these differences in findings could be attributed to the fact that the glycolytic inhibitors prevent the accumulation of H +, which also inhibits respiration. However, the data of the workers who find a release of the Crabtree effect by an inhib ...
Production and Characterization of Islet Amyloid Polypeptide Using
... IAPP is a proteinaceous hormone that comprises 37 amino acid residues. It is secreted along with insulin from the pancreatic β-cells to help it regulate the uptake and removal of glucose in the bloodstream. IAPP has been observed in the amyloid deposits found in pancreatic β-cells of most patients s ...
... IAPP is a proteinaceous hormone that comprises 37 amino acid residues. It is secreted along with insulin from the pancreatic β-cells to help it regulate the uptake and removal of glucose in the bloodstream. IAPP has been observed in the amyloid deposits found in pancreatic β-cells of most patients s ...
Cell culture
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Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.