Branching morphogenesis of the Drosophila tracheal system. Annual Review of Cell Developmental Biology 19, 623-647. pdf
... the events that follow to create the larval tracheal network occur by cell migration and changes in cell size, shape, and intercellular contacts. At 7 h AEL, primary branches begin to bud from the tracheal sac at stereotyped positions (Figure 1b,g). At each bud site, a pair of tracheal cells migrate ...
... the events that follow to create the larval tracheal network occur by cell migration and changes in cell size, shape, and intercellular contacts. At 7 h AEL, primary branches begin to bud from the tracheal sac at stereotyped positions (Figure 1b,g). At each bud site, a pair of tracheal cells migrate ...
Adherence and uptake of Francisella into host cells
... study found that a majority of bacteria appeared to be taken up by spacious asymmetrical pseudopod loops. This was the primary mechanism used for the uptake of both live and killed bacteria, suggesting that it is dependent on a pre-formed factor on the bacterial cell surface. Interestingly, they fou ...
... study found that a majority of bacteria appeared to be taken up by spacious asymmetrical pseudopod loops. This was the primary mechanism used for the uptake of both live and killed bacteria, suggesting that it is dependent on a pre-formed factor on the bacterial cell surface. Interestingly, they fou ...
"Monoclonal Antibodies to Human Cell Surface Antigens". In
... cells. Later, the CD designations were used to describe the recognized molecules, but had to be clarified by attaching the term antigen or molecule to the designation (e.g., CD2 molecule; Knapp et al., 1989). Currently, CD2 is generally used to designate the molecule, and CD2 antibody is used to des ...
... cells. Later, the CD designations were used to describe the recognized molecules, but had to be clarified by attaching the term antigen or molecule to the designation (e.g., CD2 molecule; Knapp et al., 1989). Currently, CD2 is generally used to designate the molecule, and CD2 antibody is used to des ...
The journey of developing hematopoietic stem cells
... generating HSCs for therapeutic applications would be to provide the appropriate signals from the environment where HSCs develop. In order to generate HSCs from ES cells, hematopoietic precursors have to go through the same developmental process as they do during embryogenesis. Thus, to learn how ‘s ...
... generating HSCs for therapeutic applications would be to provide the appropriate signals from the environment where HSCs develop. In order to generate HSCs from ES cells, hematopoietic precursors have to go through the same developmental process as they do during embryogenesis. Thus, to learn how ‘s ...
Cross-Presentation Proteases in MHC Class I Presentation and
... to generate most presented peptides is the proteasome. Because the proteasome and its connection to Ag presentation has been well described elsewhere, we will cover this subject in this paper relatively briefly and provide readers with references to representatives and/or recent papers and reviews. ...
... to generate most presented peptides is the proteasome. Because the proteasome and its connection to Ag presentation has been well described elsewhere, we will cover this subject in this paper relatively briefly and provide readers with references to representatives and/or recent papers and reviews. ...
PDF
... differentiation under hypoxic conditions, we inactivated the transcription factor hypoxia-inducible factor 1␣ (HIF1␣) in mouse limb bud mesenchyme. Developmental analysis of Hif1␣-depleted limbs revealed abnormal cartilage and joint formation in the autopod, suggesting that HIF1␣ is part of a mechan ...
... differentiation under hypoxic conditions, we inactivated the transcription factor hypoxia-inducible factor 1␣ (HIF1␣) in mouse limb bud mesenchyme. Developmental analysis of Hif1␣-depleted limbs revealed abnormal cartilage and joint formation in the autopod, suggesting that HIF1␣ is part of a mechan ...
6.3. La supervivencia de las motoneuronas espinales de pollo mantenidas
... still unknown. In the present work, we describe the ability of the GDNF family members to promote chicken motoneuron survival in culture. We show the presence of Ret and GFR␣-1, GFR␣-2, and GFR␣-4 in chicken motoneurons using in situ Glial cell line-derived neurotrophic factor (GDN F), neurturin (NT ...
... still unknown. In the present work, we describe the ability of the GDNF family members to promote chicken motoneuron survival in culture. We show the presence of Ret and GFR␣-1, GFR␣-2, and GFR␣-4 in chicken motoneurons using in situ Glial cell line-derived neurotrophic factor (GDN F), neurturin (NT ...
Transcriptionally repressed germ cells lack a
... (Seydoux et al., 1996). In this organism, several newly transcribed mRNAs have been detected in somatic blastomeres as early as the 4-cell stage, but none to date have been detected in germ-line blastomeres (Seydoux and Fire, 1994; Seydoux et al., 1996). This soma-germ line difference was shown to d ...
... (Seydoux et al., 1996). In this organism, several newly transcribed mRNAs have been detected in somatic blastomeres as early as the 4-cell stage, but none to date have been detected in germ-line blastomeres (Seydoux and Fire, 1994; Seydoux et al., 1996). This soma-germ line difference was shown to d ...
Spatiotemporal distribution of different extracellular polymeric
... suggested to facilitate adhesion in the case of bacterial cells exhibiting surface motility7–9. Furthermore, the early spatial organization of this extracellular matrix in the case of living Vibrio cholerae biofilms10 has shown the complementary architectural roles of matrix proteins and extracellul ...
... suggested to facilitate adhesion in the case of bacterial cells exhibiting surface motility7–9. Furthermore, the early spatial organization of this extracellular matrix in the case of living Vibrio cholerae biofilms10 has shown the complementary architectural roles of matrix proteins and extracellul ...
Blood, Lymph, and Lymph Nodes
... for abnormalities in the cells or the progression of cell maturation. Cases in which examination of the bone marrow would be helpful include animals with a lower than normal white blood cell count, anemia, or when abnormal cells are seen on a blood smear. All blood cell types are derived from a sing ...
... for abnormalities in the cells or the progression of cell maturation. Cases in which examination of the bone marrow would be helpful include animals with a lower than normal white blood cell count, anemia, or when abnormal cells are seen on a blood smear. All blood cell types are derived from a sing ...
- Nottingham ePrints
... lineages contribute primarily to extraembryonic and embryonic tissues, respectively, leading to the formation of the conceptus. In domestic ungulates, where implantation begins after the second week of embryo development (from day 14 in pigs (Dantzer, 1985), 16 in sheep and 19 in cattle (Guillomot, ...
... lineages contribute primarily to extraembryonic and embryonic tissues, respectively, leading to the formation of the conceptus. In domestic ungulates, where implantation begins after the second week of embryo development (from day 14 in pigs (Dantzer, 1985), 16 in sheep and 19 in cattle (Guillomot, ...
Transcriptionally repressed germ cells lack a subpopulation of
... (Seydoux et al., 1996). In this organism, several newly transcribed mRNAs have been detected in somatic blastomeres as early as the 4-cell stage, but none to date have been detected in germ-line blastomeres (Seydoux and Fire, 1994; Seydoux et al., 1996). This soma-germ line difference was shown to d ...
... (Seydoux et al., 1996). In this organism, several newly transcribed mRNAs have been detected in somatic blastomeres as early as the 4-cell stage, but none to date have been detected in germ-line blastomeres (Seydoux and Fire, 1994; Seydoux et al., 1996). This soma-germ line difference was shown to d ...
Protoplast isolation - student notes
... anthers and even pollen. The isolation and culture media used vary with the species and with the tissue from which the protoplasts were isolated. Protoplasts are used in a number of ways for research and for plant improvement. They can be treated in a variety of ways (electroporation, incubation wit ...
... anthers and even pollen. The isolation and culture media used vary with the species and with the tissue from which the protoplasts were isolated. Protoplasts are used in a number of ways for research and for plant improvement. They can be treated in a variety of ways (electroporation, incubation wit ...
- Wiley Online Library
... near and n = 39 far). The average diameter of the neurites in which we tracked mitochondrial movement, determined from all of those for which we had DIC images, was 1.16 ± 0.1 μm (mean ± SEM, N = 19). Well above the diffraction limit of the point spread function (>400 nm), the apparent and real size ...
... near and n = 39 far). The average diameter of the neurites in which we tracked mitochondrial movement, determined from all of those for which we had DIC images, was 1.16 ± 0.1 μm (mean ± SEM, N = 19). Well above the diffraction limit of the point spread function (>400 nm), the apparent and real size ...
Intrinsic Regulation of Cambial Growth
... within the realm of “toy research” (Horn 1880; Sprague and Sprague 1976). Some research areas relevant to secondary growth nevertheless have begun to flourish, and a number of recent reviews have addressed questions of regulation (Fukuda and others 1998; Altmann 1999; Kost and others 1999; Lachaud a ...
... within the realm of “toy research” (Horn 1880; Sprague and Sprague 1976). Some research areas relevant to secondary growth nevertheless have begun to flourish, and a number of recent reviews have addressed questions of regulation (Fukuda and others 1998; Altmann 1999; Kost and others 1999; Lachaud a ...
1 Electrophysiology and pacemaker function of the - AJP
... of the ion channel expression profile. Studies on the sodium and pacemaker currents have shown that their contribution to automaticity is greater in the newborn than in the adult, but this age-dependent decrease is at least partially opposed by an increased contribution of the L-type calcium current ...
... of the ion channel expression profile. Studies on the sodium and pacemaker currents have shown that their contribution to automaticity is greater in the newborn than in the adult, but this age-dependent decrease is at least partially opposed by an increased contribution of the L-type calcium current ...
H ydrop hobicity-hydrop hilicity of staphylococci
... Strain Cowan I was regarded as a “typical” S. hydrophilic (fig. 2). The strain was clumping-factor aureus strain and was strongly hydrophobic in tests negative but had a compact morphology in serum with all three hydrocarbons after growth overnight soft agar and no capsule could be seen in Indian (f ...
... Strain Cowan I was regarded as a “typical” S. hydrophilic (fig. 2). The strain was clumping-factor aureus strain and was strongly hydrophobic in tests negative but had a compact morphology in serum with all three hydrocarbons after growth overnight soft agar and no capsule could be seen in Indian (f ...
PDF
... When seedling peroxisomes are transformed to leaf peroxisomes, obsolete ICL and MLS must be degraded. In recent studies, three mechanisms have been proposed for the degradation of these proteins during post-germinative growth of Arabidopsis seedlings (Figure 1). In one mechanism [herein designated i ...
... When seedling peroxisomes are transformed to leaf peroxisomes, obsolete ICL and MLS must be degraded. In recent studies, three mechanisms have been proposed for the degradation of these proteins during post-germinative growth of Arabidopsis seedlings (Figure 1). In one mechanism [herein designated i ...
Plasma Membrane Ghosts Form Differently When Produced from
... visualized on tobacco BY-2 suspension culture cell membrane ghosts (Collings et al. 1998). Based on both actin and microtubule arrangements, these ghosts can be classified into 3 distinct types (types I, II and III respectively). As it became apparent that the variations between ghosts were not due ...
... visualized on tobacco BY-2 suspension culture cell membrane ghosts (Collings et al. 1998). Based on both actin and microtubule arrangements, these ghosts can be classified into 3 distinct types (types I, II and III respectively). As it became apparent that the variations between ghosts were not due ...
Cell Alloimmunization Hemin Controls T Cell Polarization in Sickle
... Patients with sickle cell disease (SCD) often require transfusions to treat and prevent worsening anemia and other SCD complications. However, transfusions can trigger alloimmunization against transfused RBCs with serious clinical sequelae. Risk factors for alloimmunization in SCD remain poorly unde ...
... Patients with sickle cell disease (SCD) often require transfusions to treat and prevent worsening anemia and other SCD complications. However, transfusions can trigger alloimmunization against transfused RBCs with serious clinical sequelae. Risk factors for alloimmunization in SCD remain poorly unde ...
regulation of the cytoskeleton and cell adhesion by
... Cytokinesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 478 Cell Transformation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 479 Other Functions of the Rho Family GTPases . . ...
... Cytokinesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 478 Cell Transformation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 479 Other Functions of the Rho Family GTPases . . ...
The Complex Relationship between Liver Cancer and the Cell Cycle
... orchestrates Cdk/cyclin complex formation, activation, and resulting activity. In addition, binding of Cdk inhibitor proteins limits Cdk activity. These inhibitors fall into two different families (Cip/Kip and Ink4) based on their inhibitory mechanism. The three members of the Cip/Kip family (p21Cip ...
... orchestrates Cdk/cyclin complex formation, activation, and resulting activity. In addition, binding of Cdk inhibitor proteins limits Cdk activity. These inhibitors fall into two different families (Cip/Kip and Ink4) based on their inhibitory mechanism. The three members of the Cip/Kip family (p21Cip ...
INTRODUCCIÓN
... the gene coding for PGDS1 in acidophilic granulocytes (AGs), which are functionally equivalent to mammalian neutrophils, after a prolonged in vitro activation with different pathogen associated molecular patterns (PAMPs). In contrast PGDS2 was not expressed in these cells. The functional relevance o ...
... the gene coding for PGDS1 in acidophilic granulocytes (AGs), which are functionally equivalent to mammalian neutrophils, after a prolonged in vitro activation with different pathogen associated molecular patterns (PAMPs). In contrast PGDS2 was not expressed in these cells. The functional relevance o ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.