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The humoral pattern recognition receptor PTX3 is stored in
... polyclonal Ig within neutrophils may give false positive results, as observed using antigranzyme antibody (32–34). Following the methodologies described in these studies, we observed similar levels of intracellular PTX3 using 20 and 2 of 12 ...
... polyclonal Ig within neutrophils may give false positive results, as observed using antigranzyme antibody (32–34). Following the methodologies described in these studies, we observed similar levels of intracellular PTX3 using 20 and 2 of 12 ...
High density lipoprotein-associated lysosphingolipids reduce E
... with a FITC-conjugated anti-E-selectin antibody (10 lL/mL). After washing, membrane antigen expression was measured by fluorescence activated cell sorting (FACS) with the Coulter EPICS XL cytofluorimeter (Coulter Electronics, Hialeigh, FL) in a standard configuration with a 530 nm bandpass filter using ...
... with a FITC-conjugated anti-E-selectin antibody (10 lL/mL). After washing, membrane antigen expression was measured by fluorescence activated cell sorting (FACS) with the Coulter EPICS XL cytofluorimeter (Coulter Electronics, Hialeigh, FL) in a standard configuration with a 530 nm bandpass filter using ...
Identity and activity of marine microbial populations as
... Publications ............................................................................................................................85 ...
... Publications ............................................................................................................................85 ...
Short-Lived and Phosphorylated Proteins
... same volume of uptake buffer (or low-Mes uptake buffer), and shaken for 45 min. The washing procedure was repeated once. Afterward, the medium was decanted off, the culture was topped up with fresh uptake buffer (or lowMes uptake buffer) to obtain a density of 75 to 100 mg cells mL21, and cells were ...
... same volume of uptake buffer (or low-Mes uptake buffer), and shaken for 45 min. The washing procedure was repeated once. Afterward, the medium was decanted off, the culture was topped up with fresh uptake buffer (or lowMes uptake buffer) to obtain a density of 75 to 100 mg cells mL21, and cells were ...
Diarch Symmetry of the Vascular Bundle in
... The outer tissues of dicotyledonous plant roots (i.e. epidermis, cortex, and endodermis) are clearly organized in distinct concentric layers in contrast to the diarch to polyarch vascular tissues of the central stele. Up to now, the outermost layer of the stele, the pericycle, has always been regard ...
... The outer tissues of dicotyledonous plant roots (i.e. epidermis, cortex, and endodermis) are clearly organized in distinct concentric layers in contrast to the diarch to polyarch vascular tissues of the central stele. Up to now, the outermost layer of the stele, the pericycle, has always been regard ...
The carbohydrates ofPhaeocystisand their degradation in the
... produced by colonial Phaeocystis cells. In all plants photosynthetic rates exceed metabolic demands when irradiance is optimal. The excess energy thus harvested is stored mainly as glucan. This glucan can be used as a respiratory substrate to maintain growth when irradiance is suboptimal, for exampl ...
... produced by colonial Phaeocystis cells. In all plants photosynthetic rates exceed metabolic demands when irradiance is optimal. The excess energy thus harvested is stored mainly as glucan. This glucan can be used as a respiratory substrate to maintain growth when irradiance is suboptimal, for exampl ...
Life, Death, and Cell Proliferation
... For Research Use Only. Not for use in diagnostic or therapeutic procedures. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. ©2005 Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, trans ...
... For Research Use Only. Not for use in diagnostic or therapeutic procedures. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. ©2005 Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, trans ...
Calcium Activation of ERK Mediated by Calmodulin Kinase I
... kinase receptors, and ligand- or voltage-gated ion channels (15, 16). These membrane receptors and channels stimulate the Ras/Rap family of small G proteins that in turn trigger a complex cascade of protein kinases terminating in activation of the MAP kinase family including the ERKs (17–20). The re ...
... kinase receptors, and ligand- or voltage-gated ion channels (15, 16). These membrane receptors and channels stimulate the Ras/Rap family of small G proteins that in turn trigger a complex cascade of protein kinases terminating in activation of the MAP kinase family including the ERKs (17–20). The re ...
The Lyn kinase C-lobe mediates Golgi export of Lyn through
... experiments with GST, GST-C-lobe or GST-C-lobe-mt, and proteins pulled down after incubation for 2 hours at 4°C were stained with Coomassie brilliant blue (CBB). An arrow indicates a protein at 70 kDa that preferentially associated with the C-lobe (wild-type). Asterisks indicate a protein derived fr ...
... experiments with GST, GST-C-lobe or GST-C-lobe-mt, and proteins pulled down after incubation for 2 hours at 4°C were stained with Coomassie brilliant blue (CBB). An arrow indicates a protein at 70 kDa that preferentially associated with the C-lobe (wild-type). Asterisks indicate a protein derived fr ...
Coordination of Cellular Pattern-Generating Circuits that Control
... vpre ⬎ Vth and then decays slowly (at a rate proportional to (⑀2/k)) when vpre ⬍ Vth. The equations for v1B, n1B, and s1B are the same as those for cell 1A, with all of the A’s replaced by B’s. When there is no intersegmental coupling present, the voltages of the local circuit cells exhibit stable “ ...
... vpre ⬎ Vth and then decays slowly (at a rate proportional to (⑀2/k)) when vpre ⬍ Vth. The equations for v1B, n1B, and s1B are the same as those for cell 1A, with all of the A’s replaced by B’s. When there is no intersegmental coupling present, the voltages of the local circuit cells exhibit stable “ ...
PDF - Bezanilla Lab
... was found in young, actively growing cells of protonemal and gametophore tissue. The isolation of P. patens mutants resistant to the auxin analog, 1-naphthalene acetic acid (NAR mutants) (4) reveals a connection between auxin sensitivity and cytokinin production. When category 2 NAR mutants are trea ...
... was found in young, actively growing cells of protonemal and gametophore tissue. The isolation of P. patens mutants resistant to the auxin analog, 1-naphthalene acetic acid (NAR mutants) (4) reveals a connection between auxin sensitivity and cytokinin production. When category 2 NAR mutants are trea ...
Structure and Organelles
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
Pre-operative HBO treatment led to enhanced expression of
... of Zamboni’s fixative (2% paraformaldehyde and 15% picric acid in 0.01 M phosphate buffered saline (PBS) at pH 7.4. Livers of all animals were removed and postfixed in the same fixative for 2-5 hours at 4, then transferred to 0.01M PBS. Following an overnight cryoprotection in 20% sucrose, livers w ...
... of Zamboni’s fixative (2% paraformaldehyde and 15% picric acid in 0.01 M phosphate buffered saline (PBS) at pH 7.4. Livers of all animals were removed and postfixed in the same fixative for 2-5 hours at 4, then transferred to 0.01M PBS. Following an overnight cryoprotection in 20% sucrose, livers w ...
alters cell wall construction
... Cadmium (Cd), one of the most toxic heavy metals, inhibits many cellular and physiological processes in plants. Here, the involvement of cytoplasmic Ca2+ gradient and actin filaments (AFs) in vesicular trafficking, cell wall deposition and tip growth was investigated during root (hair) development of ...
... Cadmium (Cd), one of the most toxic heavy metals, inhibits many cellular and physiological processes in plants. Here, the involvement of cytoplasmic Ca2+ gradient and actin filaments (AFs) in vesicular trafficking, cell wall deposition and tip growth was investigated during root (hair) development of ...
Nutrients and growth factors in mTORC1 activation
... which renders a glutamine to leucine change in amino acid 66 in the RagA protein sequence. This point-mutant form of RagA protein is constitutively bound to GTP (RagAGTP ) and allowed us generate mice with constitutive nutrient-dependent activation of mTORC1 in every cell. We first obtained MEFs (mo ...
... which renders a glutamine to leucine change in amino acid 66 in the RagA protein sequence. This point-mutant form of RagA protein is constitutively bound to GTP (RagAGTP ) and allowed us generate mice with constitutive nutrient-dependent activation of mTORC1 in every cell. We first obtained MEFs (mo ...
Paramecium as a bioassay system for elucidation of cytotoxicity and
... cytotoxicity effect on both cellular properties in all experiments performed in this study. However, we have found strong cytotoxic effect of CNF in the cell division tests. In this test we used culture medium containing lettuce juice and nutritive bacteria as described in “Materials and Methods”. T ...
... cytotoxicity effect on both cellular properties in all experiments performed in this study. However, we have found strong cytotoxic effect of CNF in the cell division tests. In this test we used culture medium containing lettuce juice and nutritive bacteria as described in “Materials and Methods”. T ...
Co-Expression of Arabidopsis thaliana Phytochelatin Synthase and Treponema denticola
... 2006). Given these studies, the goal of this work was to coexpress cysteine desulfhydrase and AtPCS in S. cerevisiae to elevate the intracellular accumulation of arsenic via the formation of PC–arsenic–sulfide complexes. We first investigated whether cysteine desulfhydrase expression (BY4742/CysDes) c ...
... 2006). Given these studies, the goal of this work was to coexpress cysteine desulfhydrase and AtPCS in S. cerevisiae to elevate the intracellular accumulation of arsenic via the formation of PC–arsenic–sulfide complexes. We first investigated whether cysteine desulfhydrase expression (BY4742/CysDes) c ...
responses of cultured cardiac myocytes to lysosomotropic
... Primary cultures of isolated myocytes were prepared from hearts of foetuses of timed pregnant Sprague-Dawley rats (Holtzman, Madison, WI). Whole hearts obtained from 16-day-old embryos were minced in adhesion salt buffer (0-2g/lMgSO4-7H 2 O, 6-8g/lNaCl; O4g/1KC1; l-5g/lNaH 2 PO 2 -H 2 O; 1 g/1 gluco ...
... Primary cultures of isolated myocytes were prepared from hearts of foetuses of timed pregnant Sprague-Dawley rats (Holtzman, Madison, WI). Whole hearts obtained from 16-day-old embryos were minced in adhesion salt buffer (0-2g/lMgSO4-7H 2 O, 6-8g/lNaCl; O4g/1KC1; l-5g/lNaH 2 PO 2 -H 2 O; 1 g/1 gluco ...
Investigation of CNT-induced Escherichia coli Lysis and Protein
... This research investigated the use of carbon nanotubes (CNTs) as a treatment to increase the permeability of a bacterial cell wall. Recombinant Escherichia coli BL21 (DE3) containing a plasmid that expressed Green Fluorescent Protein (GFP) and -lactamase were exposed to CNTs under various levels of ...
... This research investigated the use of carbon nanotubes (CNTs) as a treatment to increase the permeability of a bacterial cell wall. Recombinant Escherichia coli BL21 (DE3) containing a plasmid that expressed Green Fluorescent Protein (GFP) and -lactamase were exposed to CNTs under various levels of ...
The cell biology of lignification in higher plants
... Lignin (Latin lignum ‘wood’) is a polyphenolic polymer deposited directly in the cell wall of specialized cells. It is not only restricted to plant woody tissues but represents an integral feature ensuring the proper cellular function of many other cell types in different tissues/organs of the plant ...
... Lignin (Latin lignum ‘wood’) is a polyphenolic polymer deposited directly in the cell wall of specialized cells. It is not only restricted to plant woody tissues but represents an integral feature ensuring the proper cellular function of many other cell types in different tissues/organs of the plant ...
Protection of Drosophila chromosome ends with minimal telomere
... (Dubruille et al. 2010), suggesting that this capping protein is required to protect telomeres at all stages of spermatogenesis. As cav is essential for viability, we designed a partial rescue strategy to stably and specifically reduce its expression in the male germline. We generated flies expressi ...
... (Dubruille et al. 2010), suggesting that this capping protein is required to protect telomeres at all stages of spermatogenesis. As cav is essential for viability, we designed a partial rescue strategy to stably and specifically reduce its expression in the male germline. We generated flies expressi ...
currently Final program
... Wetlands are ecosystems in which the water table is permanently or periodically close to the soil surface. These water-saturated terrestrial environments, such as boreal Sphagnum-dominated peat bogs and lichen-dominated tundra peatlands, are abundantly colonized by members of the phylum Planctomycet ...
... Wetlands are ecosystems in which the water table is permanently or periodically close to the soil surface. These water-saturated terrestrial environments, such as boreal Sphagnum-dominated peat bogs and lichen-dominated tundra peatlands, are abundantly colonized by members of the phylum Planctomycet ...
Origin of the zebrafish endocrine and exocrine
... merged (Field et al., 2003; schematized in Fig. 1) to form a single pancreatic islet of endocrine cells, surrounded by exocrine tissue, with the entire structure localized to the right side of the ventral midline. Molecular markers reveal that pancreatic development is well under way at stages that ...
... merged (Field et al., 2003; schematized in Fig. 1) to form a single pancreatic islet of endocrine cells, surrounded by exocrine tissue, with the entire structure localized to the right side of the ventral midline. Molecular markers reveal that pancreatic development is well under way at stages that ...
Comprehensive Analysis of Human Cytomegalovirus MicroRNA
... and 60uC for 30 s. Expression of HCMV-US29 was assessed by qRT-PCR with specific F primer (59-CGACGAGACAACAATGAC39) and R primer (59-AATTGACGGTCCACTGAG39) as described above. Expression levels of 5sRNA were measured to confirm consistency of RNA extraction and amplification between ...
... and 60uC for 30 s. Expression of HCMV-US29 was assessed by qRT-PCR with specific F primer (59-CGACGAGACAACAATGAC39) and R primer (59-AATTGACGGTCCACTGAG39) as described above. Expression levels of 5sRNA were measured to confirm consistency of RNA extraction and amplification between ...
PDF
... spectrometry using stable isotope labeling [15], we here used label-free quantitative mass spectrometry empowered by the LFQ algorithm of MaxQuant [20–21]. We identified 4814 protein groups (each protein group may contain multiple proteins that are indistinguishable by mass spectrometry as they shar ...
... spectrometry using stable isotope labeling [15], we here used label-free quantitative mass spectrometry empowered by the LFQ algorithm of MaxQuant [20–21]. We identified 4814 protein groups (each protein group may contain multiple proteins that are indistinguishable by mass spectrometry as they shar ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.