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Light Control of Plasma Membrane Recruitment Using
Light Control of Plasma Membrane Recruitment Using

... interaction (Spencer et al., 1993) offers the opportunity to activate the association of two intracellular species. However, this high-affinity interaction is slow to dissociate, and thus the resulting control is poorly reversible (Terrillon and Bouvier, 2004). More recently, inputs have been design ...
Maternal Torso-Like Coordinates Tissue Folding During
Maternal Torso-Like Coordinates Tissue Folding During

... acquisition of form. While the cellular shape changes that underlie these tissue movements are well characterised, the mechanism(s) and molecules that coordinate them remain poorly understood. Here we report that the Drosophila perforin-like protein, Torso-like (Tsl), long known for its extracellula ...
Flow Cell Design for Effective Biosensing
Flow Cell Design for Effective Biosensing

Practice Test Answer Key
Practice Test Answer Key

... ____ 11. You can tell the difference between animal cells and plant cells because only plant cells have a. cell walls. c. cell membranes. b. vacuoles. d. cytoplasm. ____ 12. The chloroplasts in a plant cell a. manufacture food for the cell. b. control the amount of water entering the cell. c. remove ...
Regulation of Metabolic Events during Embryo Development
Regulation of Metabolic Events during Embryo Development

... Gas chromatography coupled with time-of-flight mass spectrometry (GC/TOFMS) was used to identify important metabolic changes during development of somatic embryos in embryogenic cell lines of Norway spruce. The studied cell lines exhibited normal, aberrant and blocked development of embryos. The res ...
Berlin-Buch - Max Delbrück Center for Molecular Medicine
Berlin-Buch - Max Delbrück Center for Molecular Medicine

... Einwerbung von Drittmitteln angewiesen, die einen großen Teil seiner effektiven Forschungsmittel ausmachen. ...
Enhancement of Murine Lymphokine-activated Killer Cell Activity by
Enhancement of Murine Lymphokine-activated Killer Cell Activity by

... (12, 13). Because it is weakly immunogenic and highly tumorigenic, the major biological characteristics mimicking that of human disease, JC is a more suitable model for experimental immunotherapy than other chemically or virally induced mam mary tumor models. Using JC as the target, we have demon st ...
Characterization of Chloroplast Division Using the Arabidopsis
Characterization of Chloroplast Division Using the Arabidopsis

... plants. To our knowledge, this is the first time the chloroplast division mechanism has been described in wild-type Arabidopsis. Chloroplast division in Arabidopsis occurs by binary fission following a sequence of changes very similar to those previously observed in wheat chloroplasts (Leech et al., ...
Microsoft Word
Microsoft Word

... mitochondrial encephalomyopathies and neuropathies, and are associated with the pathogenesis of a variety of complex disorders including heart disease, neurodegenerative diseases such as Parkinson’s, Alzheimer’s and Huntington’s, and other neurological disorders [4]. Oxidative damage to mitochondria ...
Polyamines in embryogenic cultures of Norway spruce (Picea abies
Polyamines in embryogenic cultures of Norway spruce (Picea abies

... the same group of loosely organized suspensor cells were commonly observed. Each tissue clump (150-200 mg fresh weight) produced 40-60 somatic embryos within 6-8 weeks of transfer to the maturation medium (Figure 2B). Usually most of these embryos turned green on maturity and germinated into seedlin ...
MycoplasMa Quality control
MycoplasMa Quality control

... 60 mycoplasma species, including the eight species most likely to contaminate cell cultures. All components required for the PCR reaction are provided and have been optimized for amplification. Browse our collection of titered mycoplasma reference strains and quantitative nucleic acids at www.atcc.o ...
Connective Tissue
Connective Tissue

... A tight junction is formed by the fusion of the outer layers of two plasma membranes. Tight junctions prevent the diffusion of fluids and solutes between the cells. A continuous adhesion belt lies deep to the tight junction. This belt is tied to the microfilaments of the terminal web. © 2012 Pearson ...
Exosomes with major histocompatibility complex class II and
Exosomes with major histocompatibility complex class II and

... on the cell surface, such as the tetraspan proteins CD63 and CD82 [7], which interact with membrane proteins, such as integrins and human leukocyte antigen (HLA)-DR [10]. Exosomes may not only have a role in T-cell activation, but may also function as a communicator between cells in the immune syste ...
NADPH oxidases as electrochemical generators to produce ion
NADPH oxidases as electrochemical generators to produce ion

... the neutral proteases [62], and that these conditions are entirely inappropriate for the peroxidatic activities of MPO [63]. It is important to note that the enzymology of MPO is complex and that it can function other than as a peroxidase, having superoxide dismutase [28] and catalatic [64] actions. ...
40 | the circulatory system
40 | the circulatory system

... occurs through the skin during pulmonary circulation and is referred to as pulmocutaneous circulation. As shown in Figure 40.4b, amphibians have a three-chambered heart that has two atria and one ventricle rather than the two-chambered heart of fish. The two atria (superior heart chambers) receive b ...
The bacterial divisome: ready for its close-up
The bacterial divisome: ready for its close-up

Ionic and Osmotic Effects of NaCl-Induced
Ionic and Osmotic Effects of NaCl-Induced

... The detrimental effects of high concentrations of salt on plants can be observed at the whole-plant level as the death of plants and/or decreases in productivity. Reductions in plant growth due to salt stress are often associated with decreases in photosynthetic activities, such as the electron tran ...
Ultrastructure of the Pulmonary Alveolar Cells of Rats Exposed to
Ultrastructure of the Pulmonary Alveolar Cells of Rats Exposed to

... double stained with uranyl acetate and lead citrate. Stained tissue sections were observed with a transmission electron microscope (JEOL, 100 CX) operating at 80 kv. RESULTS In the exposed animals, alveolar pneumocytes were the most affected pulmonary structures. Pneumocytes type II were more altere ...
The 2015 World Health Organization Classification of Tumors of the
The 2015 World Health Organization Classification of Tumors of the

Shared versus Specialized Glycinergic Spinal Interneurons in Axial
Shared versus Specialized Glycinergic Spinal Interneurons in Axial

... focusing and CCD image capture, and tracings were made directly from these images (Corel Draw v. 11). We only included cells filled well enough with dye to conclusively identify them based upon morphological features characteristic of the cell type. More detailed morphology of cell types and their p ...
1 The LF1 Gene of Chlamydomonas reinhardtii Encodes
1 The LF1 Gene of Chlamydomonas reinhardtii Encodes

... deflagellation, more than 35% of the lf1 cells remained aflagellate (data not shown) indicating that in addition to the flagellar length defect, these cells have either a flagellar assembly defect or a defect in the response to flagellar amputation. The lf1 mutation also resulted in abnormal cell mo ...
Phosphatidylinositol 4-Phosphate Formation at ER Exit Sites
Phosphatidylinositol 4-Phosphate Formation at ER Exit Sites

... (Sac1C392S) had no effect (Figure 2G). The inhibitory effect was reproduced in morphological ER export assays. Sac1 quantitatively delayed the cytosol-dependent mobilization of VSV-Gts to VTCs as analyzed by IF in permeabilized VSV-Gts expressing cells (Figure 2H). Importantly, Sac1 mediated delay o ...
Classifying Monerans and Protists
Classifying Monerans and Protists

... Fortunately, most bacteria are quite harmless,... ...but some, such as streptococcus bacteria, can give you a bad case of strep throat. Many different types of bacteria are used in making food. For example, a type of bacteria called acidophilus is used in making yogurt. Bacteria also play a very imp ...
NAIP5/NLRC4 Inflammasomes Compounds Inhibit the NLRP1
NAIP5/NLRC4 Inflammasomes Compounds Inhibit the NLRP1

... line of defense for the innate immune response, initiate a cascade of other immunological responses. Inflammasome activation is often accompanied by a caspase-1–dependent rapid cell death known as pyroptosis (for reviews, see Refs. 1, 2). Not surprisingly, inflammasomes and the innate immune respons ...
Theme 1 Molecules, Cells and the Basis for Disease
Theme 1 Molecules, Cells and the Basis for Disease

... is propagated through a “hard-wire” protein network that links the cell membrane with the nuclear envelope, through the cytoskeleton. The LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, formed by the association of SUN and nesprin proteins, allows a physical connection between the intermed ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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