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... sponse to RBC loss, have a longer lifespan. Without performing a correction for these so-called ‘shift’ reticulocytes, the ...
... sponse to RBC loss, have a longer lifespan. Without performing a correction for these so-called ‘shift’ reticulocytes, the ...
Thesis Reference - Archive ouverte UNIGE
... Contrary to what was originally thought, our innate immune system is not totally non-specific. It can make the difference between our self-signatures and many different Pathogen-Associated Molecular Patterns (PAMPs) using a broad set of Pathogen Recognition Receptors (PRRs). One important family of ...
... Contrary to what was originally thought, our innate immune system is not totally non-specific. It can make the difference between our self-signatures and many different Pathogen-Associated Molecular Patterns (PAMPs) using a broad set of Pathogen Recognition Receptors (PRRs). One important family of ...
Sox3 - Prodinra
... central nervous system (CNS). Indeed, much of the CNS will develop from a subset of the non-ingressing cells later specified as neural precursors. Therefore, it is crucial to identify not only those factors that induce cell ingression at gastrulation but also those that prevent it, as protection fro ...
... central nervous system (CNS). Indeed, much of the CNS will develop from a subset of the non-ingressing cells later specified as neural precursors. Therefore, it is crucial to identify not only those factors that induce cell ingression at gastrulation but also those that prevent it, as protection fro ...
An Auxin Gradient and Maximum in the Arabidopsis
... Since each technical replicate consisted of a known but variable number of protoplasts (usually ;150,000 to 200,000) of different sizes, we normalized their IAA concentrations according to their weight, calculated from size measurements, as follows. First, the diameter of the GFP-expressing protopla ...
... Since each technical replicate consisted of a known but variable number of protoplasts (usually ;150,000 to 200,000) of different sizes, we normalized their IAA concentrations according to their weight, calculated from size measurements, as follows. First, the diameter of the GFP-expressing protopla ...
High levels of tRNA abundance and alteration of tRNA charging by
... charging levels of tRNAs, in particular those coding for hydrophobic amino acids. These results suggest that tRNA properties are altered in MM to accommodate for its increased need for protein translation, and that proteasome inhibition directly impacts protein synthesis in MM through effects on tRN ...
... charging levels of tRNAs, in particular those coding for hydrophobic amino acids. These results suggest that tRNA properties are altered in MM to accommodate for its increased need for protein translation, and that proteasome inhibition directly impacts protein synthesis in MM through effects on tRN ...
Actin branching in the initiation and maintenance of lamellipodia
... organized in a diagonal array. The thickness of Rac induced lamellipodia was generally less than observed in fibroblasts used in our previous study (Urban et al, 2010) and resulted in a corresponding improvement in resolution. Actin filaments were tracked through the tomograms using both manual and ...
... organized in a diagonal array. The thickness of Rac induced lamellipodia was generally less than observed in fibroblasts used in our previous study (Urban et al, 2010) and resulted in a corresponding improvement in resolution. Actin filaments were tracked through the tomograms using both manual and ...
Cloning, Expression, and Functional Characterization of TL1A-Ig
... transmembrane protein that has a cytoplasmic death domain. TNFRSF25 is expressed primarily by T lymphocytes, including CD4+, CD8+, and NKT cells and is efficiently upregulated after T cell stimulation. The natural ligand for TNFRSF25, TL1A, is a type II transmembrane protein can be subsequently clea ...
... transmembrane protein that has a cytoplasmic death domain. TNFRSF25 is expressed primarily by T lymphocytes, including CD4+, CD8+, and NKT cells and is efficiently upregulated after T cell stimulation. The natural ligand for TNFRSF25, TL1A, is a type II transmembrane protein can be subsequently clea ...
Chapter 4 - A Tour of the Cell 08-09
... parts it needs to survive and reproduce The maximum size of a cell is limited by the amount of surface needed to obtain nutrients from the environment and dispose of wastes. • The ratio of surface are to volume imposes limits on cell size • Muscle and nerve cells can be very long because they are th ...
... parts it needs to survive and reproduce The maximum size of a cell is limited by the amount of surface needed to obtain nutrients from the environment and dispose of wastes. • The ratio of surface are to volume imposes limits on cell size • Muscle and nerve cells can be very long because they are th ...
Topological schemas of cognitive maps and spatial learning in the
... information captured by the readout neurons in the hippocampal cell assemblies. Since correlating neuronal spiking with geometrical properties of the representing space sometimes produces useful interpretations of electrophysiological data, most authors assume that the spiking patterns of place cell ...
... information captured by the readout neurons in the hippocampal cell assemblies. Since correlating neuronal spiking with geometrical properties of the representing space sometimes produces useful interpretations of electrophysiological data, most authors assume that the spiking patterns of place cell ...
form 1 april, 2012 holiday assignment questions
... Introduction to Biology, Classification I and The Cell (Microscopy, parts and functions of cell parts, cell specialization, Tissues, Organs and Organ systems) 1. Define the term cell physiology (2 mks) 2. Explain the difference between: a) Turgor pressure and wall pressure (2 mks) b) Osmotic pressur ...
... Introduction to Biology, Classification I and The Cell (Microscopy, parts and functions of cell parts, cell specialization, Tissues, Organs and Organ systems) 1. Define the term cell physiology (2 mks) 2. Explain the difference between: a) Turgor pressure and wall pressure (2 mks) b) Osmotic pressur ...
Accepted so July I979 - Journal of General Virology
... biochemical steps by which the virus genome and the process of virus replication gives rise to the diseased state. For certain diseases, studies using light microscopy provide a useful bridge between macroscopic observations and electron microscopy and biochemical studies of diseased cells. Turnip y ...
... biochemical steps by which the virus genome and the process of virus replication gives rise to the diseased state. For certain diseases, studies using light microscopy provide a useful bridge between macroscopic observations and electron microscopy and biochemical studies of diseased cells. Turnip y ...
Churchill, a Zinc Finger Transcriptional Activator, Regulates the
... ChCh may normally act with a cofactor. These results suggest that Churchill may be a DNA binding protein. Using an in vitro DNA binding selection assay, we determined the optimal binding sequences for ChCh protein from a pool of random oligonucleotides flanked by constant sequences. Comparison of th ...
... ChCh may normally act with a cofactor. These results suggest that Churchill may be a DNA binding protein. Using an in vitro DNA binding selection assay, we determined the optimal binding sequences for ChCh protein from a pool of random oligonucleotides flanked by constant sequences. Comparison of th ...
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
... Plant cell vacuoles may have storage or lytic functions, but biochemical markers specific for the tonoplasts of functionally distinct vacuoles are poorly defined. Here, we use antipeptide antibodies specific for the tonoplast intrinsic proteins a-TIP, g-TIP, and d-TIP in confocal immunofluorescence ...
... Plant cell vacuoles may have storage or lytic functions, but biochemical markers specific for the tonoplasts of functionally distinct vacuoles are poorly defined. Here, we use antipeptide antibodies specific for the tonoplast intrinsic proteins a-TIP, g-TIP, and d-TIP in confocal immunofluorescence ...
Cell death by autophagy: facts and apparent artefacts
... revealed,13 and it will be important to expand on this to understand how these signals are integrated. Autophagic Cell Death Observations that in many instances dying cells have increased autophagic markers and morphological features of autophagy originally led to the proposal of autophagic cell dea ...
... revealed,13 and it will be important to expand on this to understand how these signals are integrated. Autophagic Cell Death Observations that in many instances dying cells have increased autophagic markers and morphological features of autophagy originally led to the proposal of autophagic cell dea ...
Escaping Underground Nets: Extracellular DNases Degrade
... dense microbial community of the soil, unprotected by cuticle or bark. However, roots are defended by tiles of loosely attached secretory cells called root border cells, which produce a matrix of proteins, polysaccharide and DNA [1,2]. It has long been known that plants deposit DNA into soil [3–6], ...
... dense microbial community of the soil, unprotected by cuticle or bark. However, roots are defended by tiles of loosely attached secretory cells called root border cells, which produce a matrix of proteins, polysaccharide and DNA [1,2]. It has long been known that plants deposit DNA into soil [3–6], ...
(9E10): sc-40 - Santa Cruz Biotechnology
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
First description of giant Archaea (Thaumarchaeota)
... host (Fig. 3B). The putative ectosymbionts are rodshaped (Fig. 3E–H) with a double membrane characteristic of Gram-negative bacteria (Fig. 3A–B). They are 1.5–4.5 mm long and 0.3–0.5 mm wide (Fig. 3B and E–F). The ovoid shapes observed in thin sections (Fig. 3A–B) are caused by the orientation of th ...
... host (Fig. 3B). The putative ectosymbionts are rodshaped (Fig. 3E–H) with a double membrane characteristic of Gram-negative bacteria (Fig. 3A–B). They are 1.5–4.5 mm long and 0.3–0.5 mm wide (Fig. 3B and E–F). The ovoid shapes observed in thin sections (Fig. 3A–B) are caused by the orientation of th ...
Ezrin: a protein requiring conformational activation to link
... endothelia express moesin (Berryman et al., 1993; SchwartzAlbiez et al., 1995). In a careful study of liver tissue, Amieva et al. (1994) have shown that hepatocytes only express radixin, that the epithelial cells lining the bile ducts express both ezrin and radixin, and that the endothelial cells on ...
... endothelia express moesin (Berryman et al., 1993; SchwartzAlbiez et al., 1995). In a careful study of liver tissue, Amieva et al. (1994) have shown that hepatocytes only express radixin, that the epithelial cells lining the bile ducts express both ezrin and radixin, and that the endothelial cells on ...
Influence of Bacillus subtilis Cell Walls and EDTA on Calcite
... serves to strip the cell walls of calcium ions and other substances acquired from the growth medium. The total bacterial concentration is reported as wet weight per liter of bacterial suspension after centrifugation at 6000 rpm (RCF ) 2560g; 12) for 30 min. This measurement was used to estimate the ...
... serves to strip the cell walls of calcium ions and other substances acquired from the growth medium. The total bacterial concentration is reported as wet weight per liter of bacterial suspension after centrifugation at 6000 rpm (RCF ) 2560g; 12) for 30 min. This measurement was used to estimate the ...
c-Myc Overexpression Increases Cell Size and Impairs Cartilage
... that occasionally led to interdigital chondrogenesis. In contrast, c-myc overexpression did not interfere with other processes, such as muscle differentiation. Although based on overexpression experiments, our results suggest that endogenous c-Myc may be implicated in the control of cell size and sk ...
... that occasionally led to interdigital chondrogenesis. In contrast, c-myc overexpression did not interfere with other processes, such as muscle differentiation. Although based on overexpression experiments, our results suggest that endogenous c-Myc may be implicated in the control of cell size and sk ...
Salicylic Acid Regulates Plasmodesmata Closure
... ORCID ID: 0000-0003-4604-7974 (J-Y L). In plants, mounting an effective innate immune strategy against microbial pathogens involves triggering local cell death within infected cells as well as boosting the immunity of the uninfected neighboring and systemically located cells. Although not much is kn ...
... ORCID ID: 0000-0003-4604-7974 (J-Y L). In plants, mounting an effective innate immune strategy against microbial pathogens involves triggering local cell death within infected cells as well as boosting the immunity of the uninfected neighboring and systemically located cells. Although not much is kn ...
Localization of the Wilms` tumour protein WT1 in avian embryos
... were then rehydrated in TRIS-phosphate-buffered saline (TPBS) and the endogenous peroxidase activity was quenched by incubation for 30 min with 3% hydrogen peroxide in TPBS. After washing, non-specific binding sites were saturated for 30 min with 16% sheep serum, 1% bovine serum albumin and 0.5% Tri ...
... were then rehydrated in TRIS-phosphate-buffered saline (TPBS) and the endogenous peroxidase activity was quenched by incubation for 30 min with 3% hydrogen peroxide in TPBS. After washing, non-specific binding sites were saturated for 30 min with 16% sheep serum, 1% bovine serum albumin and 0.5% Tri ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.