Increased Susceptibility of the Sickle Cell Membrane
... are subject to many changes due to the polymerization of hemoglobin S . Some of the membrane changes include: altered ion transport, altered cytoskeletal structure, phosphatidylserine transmembrane movement, and reduced enzyme function.‘ These changes appear to be associated with the formation of de ...
... are subject to many changes due to the polymerization of hemoglobin S . Some of the membrane changes include: altered ion transport, altered cytoskeletal structure, phosphatidylserine transmembrane movement, and reduced enzyme function.‘ These changes appear to be associated with the formation of de ...
Golgi-SNARE GS28 potentiates cisplatin
... vesicle transport [28,29]. Furthermore, a protein complex consisting of the cis-Golgi vesicle receptor syntaxin 5 in complex with GS28, rbet1, rsly1, sec22b and membrin proteins has been isolated by Scheller and colleagues [30]. Interactions of these proteins strongly suggest an intermediate in ER–G ...
... vesicle transport [28,29]. Furthermore, a protein complex consisting of the cis-Golgi vesicle receptor syntaxin 5 in complex with GS28, rbet1, rsly1, sec22b and membrin proteins has been isolated by Scheller and colleagues [30]. Interactions of these proteins strongly suggest an intermediate in ER–G ...
FOG-1 - Blood Journal
... designed to target the splice-donor site of exon 4 to selectively interfere with FOG-1 mRNA processing. Using reverse transcription (RT)–PCR, we found that the injection of FOG-1 MO resulted in the production of an aberrantly spliced mRNA (supplemental Figure 2A; supplemental Table 1). Sequence anal ...
... designed to target the splice-donor site of exon 4 to selectively interfere with FOG-1 mRNA processing. Using reverse transcription (RT)–PCR, we found that the injection of FOG-1 MO resulted in the production of an aberrantly spliced mRNA (supplemental Figure 2A; supplemental Table 1). Sequence anal ...
The basic helix–loop–helix protein BETA2 interacts with p300 to
... very low levels alone or with coexpressed E47, a ubiquitous bHLH protein (Fig. 2A, columns 1,3). Cotransfecting increasing amounts of a p300 expression plasmid further potentiated BETA2-dependent transcription to >100-fold greater than the reporter alone. The failure of p300 to transactivate the rep ...
... very low levels alone or with coexpressed E47, a ubiquitous bHLH protein (Fig. 2A, columns 1,3). Cotransfecting increasing amounts of a p300 expression plasmid further potentiated BETA2-dependent transcription to >100-fold greater than the reporter alone. The failure of p300 to transactivate the rep ...
Cleavage modification did not alter early blastomere fates
... parallel to the plane of the first cleavage resulting in four internal (subscript i) and four external ...
... parallel to the plane of the first cleavage resulting in four internal (subscript i) and four external ...
Stationary phase, amino acid limitation and recovery
... well as bulk mRNA have been shown to increase during starvation in Vibrio sp. S14 (Albertson et al., 1990) and in E. coli (Albertson & Nystrom, 1994). Obviously, growth rate, growth phase and medium composition impact the stabilities of specific transcripts as well as bulk mRNA. Since mRNA degradati ...
... well as bulk mRNA have been shown to increase during starvation in Vibrio sp. S14 (Albertson et al., 1990) and in E. coli (Albertson & Nystrom, 1994). Obviously, growth rate, growth phase and medium composition impact the stabilities of specific transcripts as well as bulk mRNA. Since mRNA degradati ...
1 An amoeba phagocytosis model reveals a novel developmental
... Beeton, M.L., Atkinson, D.J. and Waterfield, N.R. (2013) An amoeba phagocytosis model reveals a novel developmental switch in the insect pathogen Bacillus thuringiensis. Journal of ...
... Beeton, M.L., Atkinson, D.J. and Waterfield, N.R. (2013) An amoeba phagocytosis model reveals a novel developmental switch in the insect pathogen Bacillus thuringiensis. Journal of ...
Redistribution of membrane proteins between the Golgi apparatus
... and movement of the ER network (Boevink et al., 1998; Liebe and Quader, 1994; Quader, 1990). Application of ¯uorescent protein technology has started to revolutionise plant cell biology and the study of the secretory pathway is no exception. It is now possible to observe in vivo the dynamic events o ...
... and movement of the ER network (Boevink et al., 1998; Liebe and Quader, 1994; Quader, 1990). Application of ¯uorescent protein technology has started to revolutionise plant cell biology and the study of the secretory pathway is no exception. It is now possible to observe in vivo the dynamic events o ...
A role for actin in regulating apoptosis/programmed cell death
... The fact that cells are selectively executed during normal development has been recognized and revisited a number of times over the last century [1]. However, it was not until the recent discovery of regulators of the most studied form of PCD, apoptosis (reviewed in [2]), that this became a ‘red hot ...
... The fact that cells are selectively executed during normal development has been recognized and revisited a number of times over the last century [1]. However, it was not until the recent discovery of regulators of the most studied form of PCD, apoptosis (reviewed in [2]), that this became a ‘red hot ...
Accepted version - QMRO Home - Queen Mary University of London
... Recent pre-clinical and clinical research has suggested that transplantation of bone marrowderived mesenchymal stromal cells (MSCs) is a promising new approach for the treatment of heart failure.[1] Although cardiomyogenic differentiation of these cells in vivo is limited, MSCs are able to induce th ...
... Recent pre-clinical and clinical research has suggested that transplantation of bone marrowderived mesenchymal stromal cells (MSCs) is a promising new approach for the treatment of heart failure.[1] Although cardiomyogenic differentiation of these cells in vivo is limited, MSCs are able to induce th ...
MCB 3020L Lab Experiment 6 Differential and Cytological Stains A
... Gram stain. Poly-beta hydroxybutyrate (bacterial fat) and other intracellular inclusions can also not stain in the Gram stain and appear as clear areas. The spore stain allows you to clearly demonstrate that the clear area in a Gram stain is or is not a spore. Since spores are refractile to stains, ...
... Gram stain. Poly-beta hydroxybutyrate (bacterial fat) and other intracellular inclusions can also not stain in the Gram stain and appear as clear areas. The spore stain allows you to clearly demonstrate that the clear area in a Gram stain is or is not a spore. Since spores are refractile to stains, ...
Control of cell differentiation and morphogenesis in amphibian
... cell differentiation, organogenesis, and even axis formation. Using the piece culture method, Smith (1987) characterized the factor responsible for mesoderm induction which is present in culture medium of Xenopus XTC cells. Slack et a/. (1987) reported that basic fibroblast growth factor (bFGF), whi ...
... cell differentiation, organogenesis, and even axis formation. Using the piece culture method, Smith (1987) characterized the factor responsible for mesoderm induction which is present in culture medium of Xenopus XTC cells. Slack et a/. (1987) reported that basic fibroblast growth factor (bFGF), whi ...
Cathepsin D released by lactating rat mammary epithelial cells is
... under an atmosphere of 95% O2 and 5% CO2. The pH of the Hanks’ medium after oxygenation was 7.5 and remained the same in the presence of mammary tissues during the whole incubation time. For the preparation of enzymatically dissociated acini, mammary fragments were incubated for 90 minutes at 37°C i ...
... under an atmosphere of 95% O2 and 5% CO2. The pH of the Hanks’ medium after oxygenation was 7.5 and remained the same in the presence of mammary tissues during the whole incubation time. For the preparation of enzymatically dissociated acini, mammary fragments were incubated for 90 minutes at 37°C i ...
Expression of GFP-fusions in Arabidopsis companion cells reveals
... globular GFP (Stokes radius ¼ 2.82 nm; Terry et al., 1995) with another globular protein, such as ubiquitin (Stokes radius about 1.2 nm) or sporamin (Stokes radius about 2.2 nm), will not result in one larger globule, but rather in a dimer of two globules. Therefore, it is not only the increased mol ...
... globular GFP (Stokes radius ¼ 2.82 nm; Terry et al., 1995) with another globular protein, such as ubiquitin (Stokes radius about 1.2 nm) or sporamin (Stokes radius about 2.2 nm), will not result in one larger globule, but rather in a dimer of two globules. Therefore, it is not only the increased mol ...
Aubé et al., J Immunol, 2014
... that neutrophils are among the first inflammatory cells recruited into the CNS, which is corroborated by studies showing the presence of PMNs in the meninges before the onset of clinical symptoms (13, 17, 18). PMNs are also increasingly recognized as having pivotal functions in driving inflammatory ...
... that neutrophils are among the first inflammatory cells recruited into the CNS, which is corroborated by studies showing the presence of PMNs in the meninges before the onset of clinical symptoms (13, 17, 18). PMNs are also increasingly recognized as having pivotal functions in driving inflammatory ...
Volume 94, issue 2 July 2011 ...
... potential mechanisms of how forces could translate into changes of cellular physiology and signaling as well as the question whether a universal mechanism of axonal elongation exists across different species. 2. Forces and axonal elongation 2.1. Forces cause axons to grow It is utterly remarkable th ...
... potential mechanisms of how forces could translate into changes of cellular physiology and signaling as well as the question whether a universal mechanism of axonal elongation exists across different species. 2. Forces and axonal elongation 2.1. Forces cause axons to grow It is utterly remarkable th ...
FERRITIN: A POTENT INHIBITOR OF VASCULAR CALCIFICATION
... ferritin. These findings have been confirmed not only by animal models but also the discovery of two cases of human HO-1 deficiency. Heme degradation results in release of iron that in turn induces the expression of ferritin. Iron is essential for almost all living organisms. It participates in a wi ...
... ferritin. These findings have been confirmed not only by animal models but also the discovery of two cases of human HO-1 deficiency. Heme degradation results in release of iron that in turn induces the expression of ferritin. Iron is essential for almost all living organisms. It participates in a wi ...
Vasopressin-stimulated Ca2 spiking in vascular smooth muscle cells
... cells within 3 min of saponin (50 g/ml) addition which suggests that ⬃95% of the dye was in the cytosol. [Ca2⫹]i measurements. Fura 2 fluorescence was measured in cell populations with a Perkin-Elmer LS50B fluorescence spectrophotometer. This instrument is equipped with a rotating filter wheel that ...
... cells within 3 min of saponin (50 g/ml) addition which suggests that ⬃95% of the dye was in the cytosol. [Ca2⫹]i measurements. Fura 2 fluorescence was measured in cell populations with a Perkin-Elmer LS50B fluorescence spectrophotometer. This instrument is equipped with a rotating filter wheel that ...
Notochord vacuoles are lysosome-related organelles that function in
... To determine the time course of notochord vacuole formation we first performed live, time-lapse imaging of vacuole expansion in wild-type (WT) embryos. All live imaging in this work was performed in the same orientation at approximately the same position along the anterior-posterior axis (Fig. 1, D ...
... To determine the time course of notochord vacuole formation we first performed live, time-lapse imaging of vacuole expansion in wild-type (WT) embryos. All live imaging in this work was performed in the same orientation at approximately the same position along the anterior-posterior axis (Fig. 1, D ...
PDF
... Finally, in a collateral experimental series the epithelial trees of 11-day rudiments have been completely separated from mesenchyme and cultured in isolation in the presence of hydrocortisone, to show the direct effect of the hormone on the epithelial glycogen. First-generation mouse hybrids from ^ ...
... Finally, in a collateral experimental series the epithelial trees of 11-day rudiments have been completely separated from mesenchyme and cultured in isolation in the presence of hydrocortisone, to show the direct effect of the hormone on the epithelial glycogen. First-generation mouse hybrids from ^ ...
Notochord vacuoles are lysosome-related organelles that function in
... To determine the time course of notochord vacuole formation we first performed live, time-lapse imaging of vacuole expansion in wild-type (WT) embryos. All live imaging in this work was performed in the same orientation at approximately the same position along the anterior-posterior axis (Fig. 1, D ...
... To determine the time course of notochord vacuole formation we first performed live, time-lapse imaging of vacuole expansion in wild-type (WT) embryos. All live imaging in this work was performed in the same orientation at approximately the same position along the anterior-posterior axis (Fig. 1, D ...
PDF version
... Copyright: © 2014 Lesilee Rose and Pierre Gönczy. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ...
... Copyright: © 2014 Lesilee Rose and Pierre Gönczy. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ...
Recent developments in the cell and molecular biology of root hairs
... relied on the isolation of mutants, particularly of Arabidopsis (see e.g. Schiefelbein and Somerville 1990, Schiefelbein et al. 1993, Baskin et al. 1992). For root hairs, Aeschbacher et al. (1994) deduced that genetic analysis of root hair development is facilitated by the fact the root hairs are di ...
... relied on the isolation of mutants, particularly of Arabidopsis (see e.g. Schiefelbein and Somerville 1990, Schiefelbein et al. 1993, Baskin et al. 1992). For root hairs, Aeschbacher et al. (1994) deduced that genetic analysis of root hair development is facilitated by the fact the root hairs are di ...
Solid Tumour Section Testis: Spermatocytic seminoma Atlas of Genetics and Cytogenetics
... selective proliferation of cells with mutations that give them growth or survival advantage and eventually to tumour formation (Goriely and Wilkie, 2012). ...
... selective proliferation of cells with mutations that give them growth or survival advantage and eventually to tumour formation (Goriely and Wilkie, 2012). ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.