Cooperation between the RING+B1-B2 and coiled-coil
... and, after 15 days of G418 selection, stained with crystal violet and evaluated for the number of resistant colonies. There was a dramatic drop in the capacity of all the cell lines to form colonies in the presence of PML overexpression (Figure 1). The level of PML expression 48 h after transfection ...
... and, after 15 days of G418 selection, stained with crystal violet and evaluated for the number of resistant colonies. There was a dramatic drop in the capacity of all the cell lines to form colonies in the presence of PML overexpression (Figure 1). The level of PML expression 48 h after transfection ...
Hox genes, neural crest cells and branchial arch patterning Paul A
... form in characteristic positions in all vertebrates suggesting that conserved localized inductive interactions underlie their formation [29]. The epibranchial placodes develop near the branchial clefts in close proximity to the cranial neural crest and the pharyngeal endoderm. Analyses of the nature ...
... form in characteristic positions in all vertebrates suggesting that conserved localized inductive interactions underlie their formation [29]. The epibranchial placodes develop near the branchial clefts in close proximity to the cranial neural crest and the pharyngeal endoderm. Analyses of the nature ...
Laboratory Guide - Indiana University Bloomington
... and function are integrated in the molecules, cells, tissues, and organs of the body. A major theme of the lectures and the focus of the laboratory studies is the area of histology, the branch of biology that centers on the cells and tissues within an organism and which, as such, serves as the found ...
... and function are integrated in the molecules, cells, tissues, and organs of the body. A major theme of the lectures and the focus of the laboratory studies is the area of histology, the branch of biology that centers on the cells and tissues within an organism and which, as such, serves as the found ...
TBX5 is required for embryonic cardiac cell cycle - UNC
... embryogenesis and a second that takes place immediately following birth (MacLellan and Schneider, 2000; Olson and Schneider, 2003; Pasumarthi and Field, 2002). The embryonic phase of cardiac proliferation begins after cardiac cell commitment, and therefore occurs coincident with, or shortly after, g ...
... embryogenesis and a second that takes place immediately following birth (MacLellan and Schneider, 2000; Olson and Schneider, 2003; Pasumarthi and Field, 2002). The embryonic phase of cardiac proliferation begins after cardiac cell commitment, and therefore occurs coincident with, or shortly after, g ...
POS-1 and germ cell specification
... To detect POS-1, embryos were immunostained by one of two protocols. Embryos treated by either protocol have detectable POS1 in the cytoplasm and in the P granules of P1 through P4. However, P granule-associated POS-1 is detected at higher levels by protocol I than by protocol II. In protocol I, emb ...
... To detect POS-1, embryos were immunostained by one of two protocols. Embryos treated by either protocol have detectable POS1 in the cytoplasm and in the P granules of P1 through P4. However, P granule-associated POS-1 is detected at higher levels by protocol I than by protocol II. In protocol I, emb ...
VP5 autocleavage is required for efficient infection by in vitro
... for aquareovirus entry, a dual-expressed VP5 and VP7 (vAcGCRV-VP5/VP7) and singly expressed VP5 or VP7 (vAcGCRV-VP5 or vAcGCRV-VP7; data not shown) recombinant baculoviruses were constructed, respectively. In addition, to determine whether the N42 residue was responsible for autocleavage of VP5, a m ...
... for aquareovirus entry, a dual-expressed VP5 and VP7 (vAcGCRV-VP5/VP7) and singly expressed VP5 or VP7 (vAcGCRV-VP5 or vAcGCRV-VP7; data not shown) recombinant baculoviruses were constructed, respectively. In addition, to determine whether the N42 residue was responsible for autocleavage of VP5, a m ...
Engineering microfluidic concentration gradient generators for
... Mass transport in convection/ diffusion based gradient generators The previous section shows how hydraulic circuit analysis is useful in determining the concentration value of individual output streams in gradient generators such as the “Christmas tree” design. However, hydraulic circuit analysis do ...
... Mass transport in convection/ diffusion based gradient generators The previous section shows how hydraulic circuit analysis is useful in determining the concentration value of individual output streams in gradient generators such as the “Christmas tree” design. However, hydraulic circuit analysis do ...
The maxillary recess of the sphenoid sinus
... The most studied recess of the sphenoid sinus seems to be the lateral one, which is highly variable. Less attention seems to be paid to the maxillary recess, extending anterior, inferior and lateral towards the maxillary sinus. Twenty patients were referred for CT of the paranasal sinuses (axial CT) ...
... The most studied recess of the sphenoid sinus seems to be the lateral one, which is highly variable. Less attention seems to be paid to the maxillary recess, extending anterior, inferior and lateral towards the maxillary sinus. Twenty patients were referred for CT of the paranasal sinuses (axial CT) ...
Engineering microfluidic concentration gradient generators for
... Mass transport in convection/ diffusion based gradient generators The previous section shows how hydraulic circuit analysis is useful in determining the concentration value of individual output streams in gradient generators such as the “Christmas tree” design. However, hydraulic circuit analysis do ...
... Mass transport in convection/ diffusion based gradient generators The previous section shows how hydraulic circuit analysis is useful in determining the concentration value of individual output streams in gradient generators such as the “Christmas tree” design. However, hydraulic circuit analysis do ...
Bacteriochlorophyll-Synthesizing Budding Bacterium
... distilled water blank. For antibiotic sensitivity tests, inoculum was spread onto 20-ml PYG agar plates by using sterile glass spreaders, antibiotic disks soaked in 500 kg of penicillin mI-’, 10pg of cycloserine mI-’, or 100 pg of vancomycin ml-’ were placed onto the agar surface, and the plates wer ...
... distilled water blank. For antibiotic sensitivity tests, inoculum was spread onto 20-ml PYG agar plates by using sterile glass spreaders, antibiotic disks soaked in 500 kg of penicillin mI-’, 10pg of cycloserine mI-’, or 100 pg of vancomycin ml-’ were placed onto the agar surface, and the plates wer ...
Viral tropism - UK-CAB
... ...so an infected CD4+ cell can join with a healthy CD4+ cell and merge. The membranes fuse and become one. This repeats, and eventually you have one large HIV-infected CD4+ cell with many nuclei - as many as 500. ...
... ...so an infected CD4+ cell can join with a healthy CD4+ cell and merge. The membranes fuse and become one. This repeats, and eventually you have one large HIV-infected CD4+ cell with many nuclei - as many as 500. ...
Chapter 17. Cytoskeleton Chapter 17. Cytoskeleton Chapter 17
... Chapter 17. Cytoskeleton • Cells are not “bags of enzymes”, but rather organized in three dimensions. • This organization is carried out by the cytoskeleton, the “bones and muscles” (and more) of cells. • Cells are thousands of times larger than a typical molecule. Therefore the cytoskeleton must lo ...
... Chapter 17. Cytoskeleton • Cells are not “bags of enzymes”, but rather organized in three dimensions. • This organization is carried out by the cytoskeleton, the “bones and muscles” (and more) of cells. • Cells are thousands of times larger than a typical molecule. Therefore the cytoskeleton must lo ...
UserFiles/files/ABC Innovative Collagen Solutions
... The most abundant protein in the human body is collagen. Collagen comes in various flavors but their structures are based on the same protein chemistry. A single collagen molecule or tropocollagen is made up of three polypeptide strands wound together in a helical manner. Multiple tropocollagens bin ...
... The most abundant protein in the human body is collagen. Collagen comes in various flavors but their structures are based on the same protein chemistry. A single collagen molecule or tropocollagen is made up of three polypeptide strands wound together in a helical manner. Multiple tropocollagens bin ...
Mobile Factories: Golgi dynamics in plant cells
... Taken together, these and other studies indicate that while plant cells utilize essentially the same machinery for vesicle transport as mammalian or yeast cells, they have adapted several of these proteins to the unique requirements of their secretory system by using them for transport between membr ...
... Taken together, these and other studies indicate that while plant cells utilize essentially the same machinery for vesicle transport as mammalian or yeast cells, they have adapted several of these proteins to the unique requirements of their secretory system by using them for transport between membr ...
Simvastatin Reduces MMP1 Expression in Human Smooth Muscle
... Objective—Activation of collagen receptors expressed by smooth muscle cells induces matrix metalloproteinase (MMP) expression. The 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) have been shown to interfere with integrin signaling, but their effects on collagen receptor-mediate ...
... Objective—Activation of collagen receptors expressed by smooth muscle cells induces matrix metalloproteinase (MMP) expression. The 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) have been shown to interfere with integrin signaling, but their effects on collagen receptor-mediate ...
SKIP controls lysosome positioning using a composite kinesin
... 2015; Woźniak and Allan, 2006). Cargo recognition by KLCs is accomplished, in part, through interaction of their TPR domains with short linear peptide ‘W-acidic’ peptide motifs that are found in many cargo adaptors including calsyntenin-1/Alcadein (CSTN1), SKIP (SifA-kinesin interacting protein), ne ...
... 2015; Woźniak and Allan, 2006). Cargo recognition by KLCs is accomplished, in part, through interaction of their TPR domains with short linear peptide ‘W-acidic’ peptide motifs that are found in many cargo adaptors including calsyntenin-1/Alcadein (CSTN1), SKIP (SifA-kinesin interacting protein), ne ...
Ruditapes decussatus (Mollusca: Bivalvia)
... the phagocytic process can be subdivided into 4 steps: (1) chemotaxis, involving the attraction of the phagocyte to the non-self material, (2) recognition, involving the attachment of the foreign material to the surface of the phagocyte, (3) internalization, and (4) intracellular degradation. Light ...
... the phagocytic process can be subdivided into 4 steps: (1) chemotaxis, involving the attraction of the phagocyte to the non-self material, (2) recognition, involving the attachment of the foreign material to the surface of the phagocyte, (3) internalization, and (4) intracellular degradation. Light ...
Det här verket är upphovrättskyddat enligt Lagen (1960
... injection of label into the follicle lumen in vivo the label can be detected in caveolae connected with the apical plasma membrane and in small vesicles in the apical cell region within a few minutes (38, 41). The recent observation on chronically stimulated thyroids that hormones are secreted in sp ...
... injection of label into the follicle lumen in vivo the label can be detected in caveolae connected with the apical plasma membrane and in small vesicles in the apical cell region within a few minutes (38, 41). The recent observation on chronically stimulated thyroids that hormones are secreted in sp ...
Evidence for an Extracellular Reception Site for Abscisic
... in the absence of external ABA (n > 180 ABA-injected guard cells in this study). Figure 2A shows two initially closed stomata following ABA microinjection and prior to exposure to light. In Figure 2B, the same two stomata are shown following incubation under white light for 135 min. In all experimen ...
... in the absence of external ABA (n > 180 ABA-injected guard cells in this study). Figure 2A shows two initially closed stomata following ABA microinjection and prior to exposure to light. In Figure 2B, the same two stomata are shown following incubation under white light for 135 min. In all experimen ...
Elevated Level of Nuclear Protein Kinase C in
... per), and the strips were immediately immersed into 75 mM phosphoric acid. The P-81 papers were washed at least 4 times with 75 mM phosphoric acid and air dried, and radioactivity retained on the papers was determined. Protein kinase C activity was calculated from the difference in 32Pincorporated i ...
... per), and the strips were immediately immersed into 75 mM phosphoric acid. The P-81 papers were washed at least 4 times with 75 mM phosphoric acid and air dried, and radioactivity retained on the papers was determined. Protein kinase C activity was calculated from the difference in 32Pincorporated i ...
Full Text - The International Journal of Developmental Biology
... Tiedemann was why he used chicken instead of amphibians as source for inducing factors. However, before the b.c. (before cloning) period, even sophisticated biochemical methods were not suitable to isolate substantial amounts of biologically active proteins from amphibian embryos, which were in the ...
... Tiedemann was why he used chicken instead of amphibians as source for inducing factors. However, before the b.c. (before cloning) period, even sophisticated biochemical methods were not suitable to isolate substantial amounts of biologically active proteins from amphibian embryos, which were in the ...
The association of sore throat and psoriasis might be explained by
... approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic disease [8], environmental factors such as trauma and stress can play a role in its elicitation [1]. Throat infections by ...
... approximately 2% of people of both sexes [5], with diminished quality of life [6] and significant co-morbidities [7]. While psoriasis has been established to be a complex genetic disease [8], environmental factors such as trauma and stress can play a role in its elicitation [1]. Throat infections by ...
PDF
... tissue-specific mutants using endoderm and/or NCC-specific Cre drivers. Simultaneous deletion with both drivers resulted in athymia at E18.5, similar to the null. By contrast, the individual tissue-specific Hoxa3 deletions resulted in small, ectopic thymi, although each had a unique phenotype. Hoxa3 ...
... tissue-specific mutants using endoderm and/or NCC-specific Cre drivers. Simultaneous deletion with both drivers resulted in athymia at E18.5, similar to the null. By contrast, the individual tissue-specific Hoxa3 deletions resulted in small, ectopic thymi, although each had a unique phenotype. Hoxa3 ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
... unacceptably high rate of misincorporation of amino acids when using conventional bacterial expression systems (You et al., 1999). The latter problem is highlighted by analysis of a Sic1 substrate containing a single lysine residue that was expressed in BL21(DE3)pLysS and used in ubiquitination assa ...
... unacceptably high rate of misincorporation of amino acids when using conventional bacterial expression systems (You et al., 1999). The latter problem is highlighted by analysis of a Sic1 substrate containing a single lysine residue that was expressed in BL21(DE3)pLysS and used in ubiquitination assa ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.