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Biology of the Cell
Biology of the Cell

... and become embedded in the phospholipid bilayer. The proteins of the plasma membrane are in large part responsible for a cell’s ability to interact with its environment. Transport proteins help molecules and ions move across the plasma membrane, either from the environment to the interior of the cel ...
File
File

... Bacteria and fungi are similar to plants in that they are enclosed by a cell wall Osmosis is often used to prevent micro – organisms such as bacteria and fungi from growing on food This prevents them from producing harmful toxins and from decaying the food 2 examples of food preservation techniques ...
Resistance of cell membranes to different detergents - MPI
Resistance of cell membranes to different detergents - MPI

... iological membranes are composed of a puzzling variety of lipids. Such diversity would be unnecessary if lipid bilayers served only as hydrophobic barriers and homogeneous twodimensional solvents for membrane proteins. As is now increasingly appreciated, membranes show extensive lipid-driven compart ...
In vitro study of chorionic and ectoplacental
In vitro study of chorionic and ectoplacental

... glucose-phosphate-isomerase hybrid enzymes in trophoblastic tissues derived from chimaeric embryos in mice. These results differ from those obtained by Baker & Mintz (1969) and Gearhart & Mintz (19726) in muscle polykaryons whose syncytial nature has been clearly established. In that case heterodime ...
Set 5 - Thorpes Education
Set 5 - Thorpes Education

... Finally, Bailyn argues that the colonies were a half-civilized hinterland of the European culture system. He is undoubtedly correct to insist that the colonies were part of an AngloAmerican empire. But to divide the empire into English core and colonial periphery, as Bailyn does, devalues the achiev ...
The Role of Receptor-Like Kinases in Regulating Cell Wall Function1
The Role of Receptor-Like Kinases in Regulating Cell Wall Function1

... tion as sensors of cell wall integrity. Both WSC and MID2 contain an extracellular domain rich in Ser/ Thr residues, a single transmembrane domain, and a small C-terminal cytoplasmic domain that interacts with ROM1 and ROM2 (Philip and Levin, 2001). Upon activation, ROM1 and ROM2 stimulate the small ...
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the

... polysaccharide xyloglucan, was used to determine the distribution of this epitope throughout the mur1 mutant of Arabidopsis. Immunofluorescent labeling of whole seedlings revealed that mur1 root hairs are stained heavily by CCRC-M1, whereas the body of the root remains unstained or only lightly stai ...
Herpesvirus Saimiri-induced Proteins in Lyrically Infected Cells. I
Herpesvirus Saimiri-induced Proteins in Lyrically Infected Cells. I

... The time-ordered appearance of proteins during lytic infection might suggest their possible regulatory functions. Blocking experiments with translation inhibitors and especially the use of amino acid analogues (Honess & Roizman, 1974; Fenwick & Walker, 1978; Bayliss & Wolf, 1981, 1982) have proved p ...
Adducin-1 is essential for mitotic spindle assembly through its
Adducin-1 is essential for mitotic spindle assembly through its

... Figure 2.  Phosphorylation of ADD1 at Ser12 and Ser355 by CDK1 is crucial for ADD1 association with mitotic spindles. (A) HeLa cells transiently expressing FLAG-ADD1 and mutants were stained for FLAG-ADD1, -tubulin, and DNA. (left) Representative confocal images from the cells in metaphase are show ...
The major salivary glands
The major salivary glands

...  The major salivary glands are paired glands with long ducts that empty into the oral cavity.  The major salivary glands, as noted above, consist of the paired parotid, submandibular, and sublingual glands.  The parotid and the submandibular glands are actually located outside the oral cavity; th ...
The Involvement of the Fibronectin Type II-like Modules
The Involvement of the Fibronectin Type II-like Modules

... with sarcosyl-extracted rCBD inclusion bodies and was then affinity purified over rCBD-AffiGel 10 (Bio-Rad) columns. Anti-peptide antibody (aHis6) to the NH2-terminal His6 fusion tag on the recombinant proteins was affinity purified as before (16). Cell Culture—Human gingival fibroblasts, kindly pro ...
MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE
MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE

... 3. When a plant cell is in a hypertonic solution: (a) The concentration of solute in the external solution is greater than the concentration of solute in the cell sap. (b) Also, the concentration of water molecules in the external solution is less than the concentration of water in the cell sap. (c ...
Antibody to bcl-2 Oncoprotein
Antibody to bcl-2 Oncoprotein

FYB Sc. Biotechnology
FYB Sc. Biotechnology

... Note: Students have learned most of the topics from this course at 10+2 level, but they need better understanding to apply or realize the relevance of these concepts with life, which is necessary while learning biotechnology. Teacher must highlight and emphasize the applications or relevance of Phys ...
Antiproliferative and apoptotic effects of O
Antiproliferative and apoptotic effects of O

... The human hepatoblastoma cell line HepG2 used in this study was obtained from Knowles et al. (21) while the human hepatocarcinoma cell line HBG was established in our laboratory and was recently described (22). Both cell lines were maintained in the following medium: 75% minimum essential medium, 25 ...
The push and pull of the bacterial cytoskeleton
The push and pull of the bacterial cytoskeleton

... depolymerization can generate a pulling force. In eukaryotic cells, for example, the energy released upon depolymerization of microtubules can be harnessed by a complex of proteins attached to chromosomes, driving their segregation [14]. Recent evidence from the bacterium Vibrio cholerae suggests an ...
Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are
Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are

... AtCDC48 has been proposed, as AtCDC48 specifically interacts with SYP31 but not with KNOLLE in vitro-binding assay in spite of the colocalization at cell-division plane between SYP31 or AtCDC48 and KNOLLE [17]. To date, only NPSN11, one RSNARE candidate for cell-plate membrane fusion machinery, has ...
CELLULAR TRANSpoRT PRACTICE
CELLULAR TRANSpoRT PRACTICE

... Read the passage below. Notice that the sentences are numbered. Then answer the questions that follow. The diffusion of water through a selectively permeable membrane is called osmosis. Like other forms of diffusion, osmosis involves the movement of a substance—water—down its concentration gradient. ...
LEH Physiology.tst
LEH Physiology.tst

... B) move food upward in the stomach to prolong digestion. C) stimulate production of hydrochloric acid. D) increase the surface of the stomach for faster digestion. ...
Wang YY, Kuang A, Russell SD, Tian HQ. 2006. In vitro fertilization
Wang YY, Kuang A, Russell SD, Tian HQ. 2006. In vitro fertilization

... exhibited preferential fertilization: one sperm containing mostly mitochondria fused with the central cell, and the other cell containing mostly plastids fused with the egg cell (Russell 1985). Sperm dimorphism and preferential fertilization in P. zeylanica suggested the presence of a recognition me ...
Supplementary Notes - Word file
Supplementary Notes - Word file

... represents a polar amino acid (Supplemental Table 2). We also noticed other features that, though not present in all sites, appear enriched in the area surrounding the Cdc5dependent phosphorylation sites. First, an aliphatic amino acid is frequently present in the +1 to +3 region, which has previous ...
Exploring How Zika Virus Attacks Cells in Developing Brains
Exploring How Zika Virus Attacks Cells in Developing Brains

... Our research using lab-grown “mini-brains” suggests that Zika virus infection causes abnormally small brains in fetuses by targeting and damaging neural progenitor cells (NPCs). The World Health Organization recently declared the clustered incidents of neurological birth defects in Zika virus outbre ...
Microbiology
Microbiology

... - Different types of cross-bridges - Are therefore resistant to penicillin - Other Archaea possess no cell wall at all. - Only an S-layer composed of protein ...
EMT in developmental morphogenesis
EMT in developmental morphogenesis

... lateral membrane adheres to each other (e.g., through adherens junctions); (5) Their basal membrane interacts with a specialized extracellular matrix, the basement membrane (e.g., through integrins and dystroglycan) [2–4,6,20]. Cells organized this way are considered to be fully epithelial (Fig. 2A) ...
The Euglena - Hamilton Local Schools
The Euglena - Hamilton Local Schools

... Euglena are unicellular organisms classified into the Kingdom Protista, and the Phylum Euglenophyta. All euglena have chloroplasts and can make their own food by photosynthesis. They are not completely autotrophic though, euglena can also absorb food from their environment. Euglena usually live in q ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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