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Supplementary Notes - Word file
Supplementary Notes - Word file

... represents a polar amino acid (Supplemental Table 2). We also noticed other features that, though not present in all sites, appear enriched in the area surrounding the Cdc5dependent phosphorylation sites. First, an aliphatic amino acid is frequently present in the +1 to +3 region, which has previous ...
Summary/Reflection of Dan Freedman`s article, Science Education
Summary/Reflection of Dan Freedman`s article, Science Education

... From here, the muscular left ventricle pumps the blood into the large artery, the aorta, through the aortic semilunar valve, and throughout the body. 1) Similar to the valves on the right side of the heart, the left AV valve prevents movement of blood into the atrium, and the semilunar valve prevent ...
viral diseases - BC Learning Network
viral diseases - BC Learning Network

... even more specific than this. Some human viruses even specialize in specific tissue. Human immunodeficiency virus (HIV) affects specific types of white blood cells and the polio virus ________________________________________________. Viruses are so specific because the capsid binds with ____________ ...
Characterization of a von Hippel Lindau Pathway Involved in
Characterization of a von Hippel Lindau Pathway Involved in

... The role of VHL in the regulation of proangiogenic factors stems from its ability to down-regulate the alpha subunit of the hypoxiainducible factor (HIF-a), which exists in at least three isoforms (HIF1a, HIF-2a, and HIF-3a; ref. 8). The VHL protein is part of an E3 ubiquitin ligase complex which ta ...
Herpesvirus Saimiri-induced Proteins in Lyrically Infected Cells. I
Herpesvirus Saimiri-induced Proteins in Lyrically Infected Cells. I

... The time-ordered appearance of proteins during lytic infection might suggest their possible regulatory functions. Blocking experiments with translation inhibitors and especially the use of amino acid analogues (Honess & Roizman, 1974; Fenwick & Walker, 1978; Bayliss & Wolf, 1981, 1982) have proved p ...
Biology Released Form - North Carolina Public Schools
Biology Released Form - North Carolina Public Schools

... BIOLOGY RELEASED FORM This diagram shows a plant cell. Which structure is found in a plant cell but is absent in an animal cell? ...
Megf10 regulates the progression of the satellite cell myogenic
Megf10 regulates the progression of the satellite cell myogenic

... cells in resting skeletal muscle. Bar, 10 μm. (C) Individual fibers from the EDL muscle of Myf5-Cre*ROSA-YFP reporter mice were isolated, freshly fixed, and stained for YFP, Pax7, and Megf10 expression. Megf10 expression was detected in Pax7+/YFP+ quiescent satellite cells but never in Pax7+/YFP− quie ...
inflammation 1 2016
inflammation 1 2016

... • Microbial sensors. • 10 mammalian types: • Can recognize bacterial products : endotoxins, lipopolysaccharides or DNA. • Can recognize viral products: RNA • They recognize a pattern: e:g liposaccharides in general, not a specific type, DNA chains not specific sequences. ...
Carbohydrates Learning Objective Carbohydrates
Carbohydrates Learning Objective Carbohydrates

... chains of glucose residues linked together by -1,4 glycosidic bonds. These chains formed by -linkages have very high tensile strength and can be digested by the enzyme cellulase, which is not inherent in mammals. 9. Glycogen: Glycogen is the storage form of glucose in animal cells. It is structura ...
Cell Biology: Theory and Laboratory Skills
Cell Biology: Theory and Laboratory Skills

... secretion resulting in loss of control of blood glucose rising to type 1 diabetes mellitus. Other examples could include type 2 diabetes, cancers, Parkinson’s disease, multiple sclerosis and deafness associated with connexon mutations. Outcome 4  Perform practical experiments related to cell biolog ...
Cell movements during epiboly and gastrulation in
Cell movements during epiboly and gastrulation in

... Materials and methods Embryos and stages Zebrafish embryos were obtained from natural spawnings and staged by cell number during early cleavage. They were dechorionated with watchmaker's forceps and kept at 28.5°C in an incubation medium of 14mM NaCl, 0.6mM KC1, 1.3mM CaCl2, lmM MgSO4, and 0.07mM so ...
cathepsin-d is required for the growth of fibroblasts in - HAL
cathepsin-d is required for the growth of fibroblasts in - HAL

... panel). In the latter case, higher magnification showed a network of interacting cells (Figure 2B, bottom panel). Cath-D-deficient CD55-/-SV40 fibroblasts grew poorly and displayed a stressed morphology of well-delineated rounded cells (Figure 2B, bottom panel). We finally compared migration of tran ...
Chapter 7 Cellular Structure and Function HUMAN SKIN HUMAN
Chapter 7 Cellular Structure and Function HUMAN SKIN HUMAN

... You have learned, according to the cell theory, that cells are the basic units of all living organisms. By observing your own body and the living things around you, you might infer that cells must exist in various shapes and sizes. You also might infer that cells differ based on the function they pe ...
PULP
PULP

... the oral cavity is only about 8.3 years. ...
Future research leaders
Future research leaders

... in Sweden today. These are exceptionally driven, innovative and skillful men and women with the potential to become the next generation of internationally recognized research leaders. Through our Future Research Leader Program, we will support and promote the work of these rising talents. As well as ...
Alief ISD Biology STAAR EOC Review
Alief ISD Biology STAAR EOC Review

... Thank You Alief ISD ...
bacteria - CNR WEB SITE
bacteria - CNR WEB SITE

... Bacteria lack the membrane-bound nuclei of eukaryotes; their DNA forms a tangle known as a nucleoid, but there is no membrane around the nucleoid, and the DNA is not bound to proteins and organized into linear pieces of chromosomes like in the eukaryotes. Bacterial DNA forms loops, called plasmids, ...
The FbaB-type fibronectin-binding protein of Streptococcus pyogenes
The FbaB-type fibronectin-binding protein of Streptococcus pyogenes

... uptake of FbaB-expressing GBS in comparison with EGFP-expressing cells (Fig. 3C), clearly demonstrating that Rac1 is an essential factor for FbaB-mediated uptake. To analyse the activation of Rac1 we used a fluorescently tagged Cdc42 and Rac1 interactive binding domain (CRIB) fused to TagRFP (CRIB-T ...
Mutations in SIPA1L3 cause eye defects through disruption of cell
Mutations in SIPA1L3 cause eye defects through disruption of cell

Plankton Biol. Ecol. 52(2), Page 67, 2005
Plankton Biol. Ecol. 52(2), Page 67, 2005

... by an order of magnitude (Kobori & Taga 1979; Hernandez ...
File - wHOOSe Learning
File - wHOOSe Learning

... A classification key is an important tool used to help identify objects and organisms. It consists of a branching set of choices organized in levels, with most levels of the key having two choices. Each level provides more specific descriptors, eventually leading to identification. A hypothesis is a ...
MES-1 and asymmetric embryo divisions
MES-1 and asymmetric embryo divisions

... Asymmetric localization of cytoplasmic factors and unequal cell division are fundamental to the development of all eukaryotes. When these mechanisms are used to generate distinct daughter cells during development, the two events must be coordinately regulated, to ensure the proper segregation of fac ...
Supplementary figure legend
Supplementary figure legend

... MELK protein expression is significantly elevated in ER-negative as compared to ER-positive breast cancer cell lines (B-C). There is a strong correlation between MELK RNA expression (measured by qRT-PCR) and protein expression (measured by western blotting) levels (D). Western blotting was performed ...
CD34+ Cells: A Comparison of Stem and Progenitor
CD34+ Cells: A Comparison of Stem and Progenitor

... Hematopoietic Stem Cells (HSCs) are defined by two characteristics: multipotency and self renewal. Multipotency refers to ability of HSCs to differentiate into any hematopoietic cell types without restriction. Self renewal is defined as the ability of an HSC to divide and produce an identical multip ...
The Cell Wall of Prokaryotes: Peptidoglycan and Related Molecules
The Cell Wall of Prokaryotes: Peptidoglycan and Related Molecules

... Gram + bacteria very sensitive to the action of penicillin and lysosome • Penicilin interferes with the final linking of the peptidoglycan rows by a peptide cross-bridge • Lysozyme is an enzyme found in tears and saliva that breaks the b-1,4-glycosidic bonds between NAM and NAG • Gram + cell wall i ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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