Cytoplasmic calcium measurements in intact higher plant cells
... demonstrate that cytoplasmic calcium levels in plant cells do change when signals are received (Hepler & Wayne, 1985; Clarkson, 1986). The first report that this does occur showed that, when Nitellopsis cells are transferred from darkness to light, the cytoplasmic calcium ion activity, measured by ...
... demonstrate that cytoplasmic calcium levels in plant cells do change when signals are received (Hepler & Wayne, 1985; Clarkson, 1986). The first report that this does occur showed that, when Nitellopsis cells are transferred from darkness to light, the cytoplasmic calcium ion activity, measured by ...
Slides - Institute for Evidence
... Bioremediation: the concept Microbes, like all life, need an ecological niche. Some get it by brawn (growing very fast)… …some by brain (living off material that others can't). Any abundant, energy-rich organic material that is hard to degrade thus provides selective pressure to evolve the machiner ...
... Bioremediation: the concept Microbes, like all life, need an ecological niche. Some get it by brawn (growing very fast)… …some by brain (living off material that others can't). Any abundant, energy-rich organic material that is hard to degrade thus provides selective pressure to evolve the machiner ...
Vacuolar system distribution in Arabidopsis tissues
... different sizes were visible in all cells. Electron microscopy observations combined with immunogold labelling experiments, carried out on similar structures in other laboratories, showed that the larger compartments are ERassociated bodies, while the smaller ones are Golgi bodies (Hawes et al., 200 ...
... different sizes were visible in all cells. Electron microscopy observations combined with immunogold labelling experiments, carried out on similar structures in other laboratories, showed that the larger compartments are ERassociated bodies, while the smaller ones are Golgi bodies (Hawes et al., 200 ...
Chapt 6 Study Guide (Word)
... CHAPTER SCOPE Many important activities that occur between cells and the extracellular environment that involve the plasma membrane are fully explored in this chapter. To a large extent the protein and phospholipid molecules that make up much of the chemical composition of the plasma membrane regula ...
... CHAPTER SCOPE Many important activities that occur between cells and the extracellular environment that involve the plasma membrane are fully explored in this chapter. To a large extent the protein and phospholipid molecules that make up much of the chemical composition of the plasma membrane regula ...
Lecture Notes
... only have 23 chromosomes and are said to be haploid (n chromosomal number). Gamete formation is by meiosis, in which the number of chromosomes is halved (from 2n to n). ...
... only have 23 chromosomes and are said to be haploid (n chromosomal number). Gamete formation is by meiosis, in which the number of chromosomes is halved (from 2n to n). ...
The Plant Extracellular Matrix: News from the Cell`s
... flotation centrifugation, and a sequencedependent glucanase was used to show that the synthase produced the expected products, cellotriosyl and cellotetraosyl unit structures, in vitro (Gibeaut and Carpita, 1994). However, a callose synthase activity was observed in the maize Golgi membranes in addi ...
... flotation centrifugation, and a sequencedependent glucanase was used to show that the synthase produced the expected products, cellotriosyl and cellotetraosyl unit structures, in vitro (Gibeaut and Carpita, 1994). However, a callose synthase activity was observed in the maize Golgi membranes in addi ...
Cloning
... inserted into an enucleated egg. After the egg containing the patient's DNA starts to divide, embryonic stem cells that can be transformed into any type of tissue would be harvested. The stem cells would be used to generate an organ or tissue that is a genetic match to the recipient. In theory, the ...
... inserted into an enucleated egg. After the egg containing the patient's DNA starts to divide, embryonic stem cells that can be transformed into any type of tissue would be harvested. The stem cells would be used to generate an organ or tissue that is a genetic match to the recipient. In theory, the ...
The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape
... developmental stages. Our current inability to visualize the cytoskeletons of intact spk1-1 trichomes has hindered our studies of SPK1 function in this cell type. The shape of the cotyledon was affected severely in the spk1-1 mutant. At 2 days after germination (DAG), wild-type cotyledons were rough ...
... developmental stages. Our current inability to visualize the cytoskeletons of intact spk1-1 trichomes has hindered our studies of SPK1 function in this cell type. The shape of the cotyledon was affected severely in the spk1-1 mutant. At 2 days after germination (DAG), wild-type cotyledons were rough ...
Different involvement of the mitochondrial, plastidial and
... to the overproduction of reactive oxygen species (ROS) in the cell. In plants, the ascorbate (ASC)-GSH cycle plays a pivotal role in controlling ROS levels and cellular redox homeostasis. Ascorbate peroxidase (APX) is the enzyme of this cycle mainly involved in ROS detoxification. In this study, the ...
... to the overproduction of reactive oxygen species (ROS) in the cell. In plants, the ascorbate (ASC)-GSH cycle plays a pivotal role in controlling ROS levels and cellular redox homeostasis. Ascorbate peroxidase (APX) is the enzyme of this cycle mainly involved in ROS detoxification. In this study, the ...
A novel microtubule-modulating noscapinoid
... Figure 1 ‘Kinder and gentler’ effects of EM011 on microtubules. Dynamic instability behavior of fluorescently labeled interphase microtubules in the thin lamellar edge of MCF-7 cells expressing GFP-tubulin was imaged by time-lapse microscopy.(ai) Time-lapse sequence of video frames, 3 s apart, showi ...
... Figure 1 ‘Kinder and gentler’ effects of EM011 on microtubules. Dynamic instability behavior of fluorescently labeled interphase microtubules in the thin lamellar edge of MCF-7 cells expressing GFP-tubulin was imaged by time-lapse microscopy.(ai) Time-lapse sequence of video frames, 3 s apart, showi ...
Microtubule and F-actin dynamics at the division site in living
... restricted to the spindle while F-actin may remain in cortical and cytoplasmic networks. In vacuolate cells, F-actin extends through phragmosomal strands of cytoplasm lying in the plane of division, and is anchored at the division site in the cell cortex to which it may guide the expanding phragmopl ...
... restricted to the spindle while F-actin may remain in cortical and cytoplasmic networks. In vacuolate cells, F-actin extends through phragmosomal strands of cytoplasm lying in the plane of division, and is anchored at the division site in the cell cortex to which it may guide the expanding phragmopl ...
ONLINE METHODS Experiments were conducted in accordance
... homology arm and the 3’ homology arm. T2A-Cre-polyA-FRT-Neo cassette-FRT was inserted into immediately before the STOP codon of MAP3K15. The vector was linearized by NotI and electroporated into embryonic stem (ES) cells (C57BL/6 background: inGenious Targeting Laboratory). Positive clones were conf ...
... homology arm and the 3’ homology arm. T2A-Cre-polyA-FRT-Neo cassette-FRT was inserted into immediately before the STOP codon of MAP3K15. The vector was linearized by NotI and electroporated into embryonic stem (ES) cells (C57BL/6 background: inGenious Targeting Laboratory). Positive clones were conf ...
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... physical characteristics as biomarkers and is a primary bottleneck in the adoption of microfluidic technologies to discriminate cells based on those characteristics. Although this type of bottom-up prediction of physical properties is currently intractable, an alternate top-down approach is to work ...
... physical characteristics as biomarkers and is a primary bottleneck in the adoption of microfluidic technologies to discriminate cells based on those characteristics. Although this type of bottom-up prediction of physical properties is currently intractable, an alternate top-down approach is to work ...
Growth Control: A Saga of Cell Walls, ROS, and
... pollen tube is induced by ROS application and is required for ROS-induced bursting. It is intriguing in this context that rbohdeficient mutant pollen tubes (see below; Boisson-Dernier et al., 2013; Lassig et al., 2014) and root hairs (Duan et al., 2010) with low internal levels of ROS also display lo ...
... pollen tube is induced by ROS application and is required for ROS-induced bursting. It is intriguing in this context that rbohdeficient mutant pollen tubes (see below; Boisson-Dernier et al., 2013; Lassig et al., 2014) and root hairs (Duan et al., 2010) with low internal levels of ROS also display lo ...
Intestinal Epithelial Cells Use Two Distinct Pathways for HLA
... Intestinal epithelial cells express a low level of HLA class II molecules constitutively, with elevated levels seen in the setting of mucosal inflammation including inflammatory bowel disease. The ability of intestinal epithelial cells to act as antigen presenting cells for ab CD41 T lymphocytes was ...
... Intestinal epithelial cells express a low level of HLA class II molecules constitutively, with elevated levels seen in the setting of mucosal inflammation including inflammatory bowel disease. The ability of intestinal epithelial cells to act as antigen presenting cells for ab CD41 T lymphocytes was ...
Chapter 4 Notes
... 4.18 Cilia and flagella move when microtubules bend • The short, numerous appendages that propel protists such as Paramecium are called cilia (singular, cilium). • Other protists may move using flagella, which are longer than cilia and usually limited to one or a few per cell. • Some cells of multi ...
... 4.18 Cilia and flagella move when microtubules bend • The short, numerous appendages that propel protists such as Paramecium are called cilia (singular, cilium). • Other protists may move using flagella, which are longer than cilia and usually limited to one or a few per cell. • Some cells of multi ...
Tissues
... • Serves as the internal framework of soft organs such as the liver, lymphatic system organs • Is made up of thin, branched reticular fibers ...
... • Serves as the internal framework of soft organs such as the liver, lymphatic system organs • Is made up of thin, branched reticular fibers ...
Rapid analysis of the extracellular matrix protein decorin using the
... Decorin is found in connective tissue where, among other roles, it helps to regulate cell growth by interacting with growth factors and collagen. After cloning of full-length human decorin cDNA into the pQE-TriSystem vector, human fibrosarcoma cells (HT-1080) were transfected, and the expression of ...
... Decorin is found in connective tissue where, among other roles, it helps to regulate cell growth by interacting with growth factors and collagen. After cloning of full-length human decorin cDNA into the pQE-TriSystem vector, human fibrosarcoma cells (HT-1080) were transfected, and the expression of ...
1-1 Test Bank Huether: Understanding Pathophysiology, 4th Edition
... 1. A cell is isolated for microscopy. It is observed to have supercoiled DNA with histones. Which of the following would also be observed? A. Single circular chromosome B. Nucleus C. Freely floating nuclear material D. No organelles ANS: B ...
... 1. A cell is isolated for microscopy. It is observed to have supercoiled DNA with histones. Which of the following would also be observed? A. Single circular chromosome B. Nucleus C. Freely floating nuclear material D. No organelles ANS: B ...
Lactobacillus plantarum displaying CCL3 chemokine in fusion with
... lactobacilli interact with DCs and thereby regulate T cell responses [23]. It is well known that Lactobacillus spp. have immunostimulatory properties that may vary between strains [24]. Because of their immunostimulatory properties, lactobacilli themselves are considered as potential vaccine adjuvan ...
... lactobacilli interact with DCs and thereby regulate T cell responses [23]. It is well known that Lactobacillus spp. have immunostimulatory properties that may vary between strains [24]. Because of their immunostimulatory properties, lactobacilli themselves are considered as potential vaccine adjuvan ...
Filtration Processes Applied in Vaccine Production
... The subject of this 3M Purification Inc.™ Application Brief is filtration applied in vaccine production. Vaccines are derived from a variety of sources including tissue extracts, bacterial cells, virus particles, recombinant mammalian, yeast and insect cell produced proteins and nucleic acids. Typic ...
... The subject of this 3M Purification Inc.™ Application Brief is filtration applied in vaccine production. Vaccines are derived from a variety of sources including tissue extracts, bacterial cells, virus particles, recombinant mammalian, yeast and insect cell produced proteins and nucleic acids. Typic ...
Effect of lithium and sodium cations on the
... Monovalent cations are very important in yeast metabolism. However, high internal concentrations of Na+ or its analogue Li+ are generally toxic to cells [1]. Lithium is highly toxic to yeast when grown in galactose medium, mainly because phosphoglucomutase, a key enzyme of galactose metabolism, is i ...
... Monovalent cations are very important in yeast metabolism. However, high internal concentrations of Na+ or its analogue Li+ are generally toxic to cells [1]. Lithium is highly toxic to yeast when grown in galactose medium, mainly because phosphoglucomutase, a key enzyme of galactose metabolism, is i ...
Slides - Evidence
... Embryonic stem cells display unlimited capacity for self-renewal and differentiation into various cell types. 1960s work on human in vitro fertilization gave rise to the modern idea of stem cells Embryonic stem cells (from mice) were not successfully cultured until 1981 Human embryonic stem cell ...
... Embryonic stem cells display unlimited capacity for self-renewal and differentiation into various cell types. 1960s work on human in vitro fertilization gave rise to the modern idea of stem cells Embryonic stem cells (from mice) were not successfully cultured until 1981 Human embryonic stem cell ...
The endocytic network in plants
... Moreover, plant cells can internalize components of their cell wall [10,11,13,15], which might provide an important paradigm of an effective mechanism for remodelling extracellular matrices in other organisms. Compartments, molecules and markers The endocytic machinery, which encompasses both molecu ...
... Moreover, plant cells can internalize components of their cell wall [10,11,13,15], which might provide an important paradigm of an effective mechanism for remodelling extracellular matrices in other organisms. Compartments, molecules and markers The endocytic machinery, which encompasses both molecu ...
Epstein-Barr virus inhibits the development of dendritic cells by
... in immunocompetent hosts. It is associated with several malignancies of hematopoietic and nonhematopoietic origin. Several lines of evidence indicate that major histocompatibility complex (MHC) class I-restricted CD8⫹ cytotoxic T lymphocytes (CTLs) are critical for the control of EBV infection. The ...
... in immunocompetent hosts. It is associated with several malignancies of hematopoietic and nonhematopoietic origin. Several lines of evidence indicate that major histocompatibility complex (MHC) class I-restricted CD8⫹ cytotoxic T lymphocytes (CTLs) are critical for the control of EBV infection. The ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.