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The Cell Membrane
The Cell Membrane

...  In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer It’s like a fluid… It’s like a mosaic… It’s the Fluid Mosaic Model! ...
Yeast Cbk1 and Mob2 Activate Daughter
Yeast Cbk1 and Mob2 Activate Daughter

... and daughters has occupied a mainstream place in molecular biology. Yeast mother-daughter asymmetry provides a tractable system to elucidate the means by which cells give rise to progeny whose gene expression differs as a consequence of internal molecular events rather than as a consequence of diffe ...
The Discovery of 5-Formylcytosine in Embryonic Stem Cell DNA
The Discovery of 5-Formylcytosine in Embryonic Stem Cell DNA

... exact mass for fC (m/zcalcd = 256.0928; Figure 1 B). To unequivocally prove that the signal is generated by the presence of fC we synthesized the fC compound independently, as reported previously by us,[10a] and co-injected a small amount of the synthetic material into the DNA digest obtained from t ...
Ultrastructural Aspects of Human and Canine Diabetic
Ultrastructural Aspects of Human and Canine Diabetic

... preceding changes may be associated with large hemorrhages which extend into the vitreous, and proliferation of new-formed capillaries into the vitreous or on the surface of the retina, so-called "retinitis proliferans."2 This condition almost inevitably leads to blindness if the patient lives long ...
De novo fatty acid synthesis controls the fate between regulatory T
De novo fatty acid synthesis controls the fate between regulatory T

... ACC on the TH17 versus Treg cell lineage development. Aside from a pronounced downregulation of IL-17 on both the transcriptional and translational level (Fig. 1d,e), naive CD4+ T cells that differentiated under TH17-polarizing conditions in the presence of SorA also exhibited reduced expression of ...
The ARP2/3 complex: giving plant cells a leading edge
The ARP2/3 complex: giving plant cells a leading edge

... Molecular and cell-biological dissection of interactions at the leading edge of an animal cell suggests that membrane protrusion could be related to a more fundamental actin polymerization-based form of motility that is exhibited by certain microorganisms and endosymbiotic subcellular organelles.(2– ...
Cytological Changes in the Ehrlich Ascites Tumor Following
Cytological Changes in the Ehrlich Ascites Tumor Following

Response of Primary Human Airway Epithelial Cells to Influenza
Response of Primary Human Airway Epithelial Cells to Influenza

LC1
LC1

... How are atoms different to each other? Atoms are different to compounds, elements and mixtures Atoms are the smallest particles known to scientists Subatomic particles control which atom is which Electrons arrange themselves in specific ways How does the periodic table help us understand atoms? The ...
Studies on Cell Adhesion and Recognition I. Extent and Specificity
Studies on Cell Adhesion and Recognition I. Extent and Specificity

... The adhesion surfaces were prepared by adsorbing different proteins on microtiter wells (see above). Unless otherwise indicated, fibronectin and different lectins were adsorbed at 10 pg/ml. The enzyme concentrations were 0.2 U/ml of Clostridium perfringens sialidase and 0.9 U/ml of 6-galactosidase ( ...
Cytokinin Fig. 15
Cytokinin Fig. 15

... Cytokinin applied to the righthand primary leaf of this bean seedling inhibited its senescence. The left-hand did not get cytokinin. ...
Unit 4 Cells, Tissues, Organs and Systems Suggested Time: 18 Hours
Unit 4 Cells, Tissues, Organs and Systems Suggested Time: 18 Hours

... • Using the format of “Pin-the-tail-on-the-donkey” game, label the parts of the microscope (209-3). • Create a foldable with a labelled diagram of the microscope and lift flaps to describe the function of each part. (209-3). ...
THE INTERACTION OF HERPES SIMPLEX VIRUS WITH MURINE
THE INTERACTION OF HERPES SIMPLEX VIRUS WITH MURINE

... stimulation observed is generally 10- to 50-fold above the background response, and is comparable in magnitude to the responses elicited by LPS in spleen cultures (Fig. 1). The DNA that is synthesized bands at a density in cesium chloride which is characteristic of mouse cellular DNA (p = 1.700 g/cm ...
Active cell migration drives the unilateral movements
Active cell migration drives the unilateral movements

... Cell tracking was performed using the tracking module of the Volocity programme (Improvision). Eight cells were manually outlined using a graphics tablet and their centroids calculated at each time point. The path taken by cells was generated using Volocity. Data on the positions of the cells were i ...
Cell-A-Brate - 6.LS.1 - Columbus City Schools
Cell-A-Brate - 6.LS.1 - Columbus City Schools

... *The content statements for sixth-grade life science are each partial components of a larger concept. The parts have been isolated in the New Learning Standards to call attention to the depth of knowledge required to build to one of biology’s important foundational theories: Modern Cell Theory. It i ...
secretion and endocytosis in insulin
secretion and endocytosis in insulin

... I n preparations ("controls") from peroxidaseinjected animals not injected with insulin, peroxidase is demonstrable in the capillaries and intercellular spaces at the earliest time studied, 30 min after injection (Fig. 3). Small peroxidase-containing bodies are also visible in the medulla cells. In ...
Developmentally Controlled Farnesylation
Developmentally Controlled Farnesylation

... not shown). The results also confirmed that the major protein detected by the antibody corresponds to AtNAP1;1, but that the antibody cross reacted with AtNAP1;2 and AtNAP1;3 as well. The observed differences in band intensity could result from a difference in expression of the AtNAP1 proteins or fr ...
Cytoskeletal rearrangements in gastric epithelial cells in response to
Cytoskeletal rearrangements in gastric epithelial cells in response to

... Helicobacter pylori causes host epithelial cell cytoskeletal rearrangements mediated by the translocation and tyrosine phosphorylation of an outer-membrane protein, CagA, and by the vacuolating cytotoxin, VacA. However, the mechanisms by which H. pylori mediates cytoskeletal rearrangements in infect ...
Epithelia and integration in sponges
Epithelia and integration in sponges

... implies being constructed of fewer parts with less hierarchical organization. In fact, sponges are usually described as lacking organs, tissues, nerves, muscle, and even epithelia. However, not only is this view not very useful for understanding what characteristics the animals have, there is also m ...
Roles of 14-3-3 and calmodulin binding in subcellular localization
Roles of 14-3-3 and calmodulin binding in subcellular localization

... (Figure 2A, panel c) and GST–14-3-3 or GST–14-3-3 K49E (Figure 2A, panel d) respectively. Similar results were obtained for the inverse experiment where Rem2 was first immunoprecipitated and the associated overexpressed GST–14-3-3 and endogenous 14-3-3 were detected using an anti-14-3-3 antibody (Fi ...
Plasmablast and Plasma Cell Production and Distribution in Trout
Plasmablast and Plasma Cell Production and Distribution in Trout

... then blocked by the addition of the blocking solution for 1 h. Biotinylated mAb 1–14 (0.5 ␮g/ml) was then added to the membrane and incubated at room temperature for 1 h on a rotary table, followed by a 1-h incubation with 30 ng/ml streptavidin-HRP (Sigma-Aldrich) in PBS. Forty milliliters of the su ...
The Distribution and Morphology Alterations of Microfilaments and
The Distribution and Morphology Alterations of Microfilaments and

... of enhanced UV-B radiation on the growth of living organisms had been extensively concentrated. Recently, lots of researches have indicated that enhanced UV-B radiation can arouse significant effects on living organisms in morphological structures, physiological processes, biochemical metabolism, ph ...
The Proteome of Native Adult Müller Glial Cells From Murine
The Proteome of Native Adult Müller Glial Cells From Murine

... neurons. Müller cells, the dominant macroglia cells of the retina, were considered passive bystanders. However, owing to their distinct morphology spanning the whole thickness of the retina and being in contact with virtually all retinal cell types enables them to fulfil a plethora of functions whi ...
Extracellular Matrix Components Regulate Cellular Polarity and
Extracellular Matrix Components Regulate Cellular Polarity and

... retina with the vitreous body [Figure 1]. Several retinal pathologies result from changes in the organization or composition of these basement membranes. These pathologies include diabetic retinopathy, retinopathy of prematurity, age related macular degeneration and proliferative vitreoretinopathy.[ ...
Divergent Roles for Maize PAN1 and PAN2 Receptor
Divergent Roles for Maize PAN1 and PAN2 Receptor

... model in which ABP1-auxin interaction at the cell surface signals through ROP2 and RIC4 to promote actindependent localized accumulation of the auxin efflux carrier PIN1, further increasing the local concentration of auxin and establishing a local feedback loop that promotes localized cell expansion ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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