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...  Lymphocytes – compose 20 – 45% of WBCs  The most important cells of the immune system  Nucleus – stains dark purple  Effective in fighting infectious organisms  Act against a specific foreign molecule (antigen) ...
Asymmetric Cell Divisions in the Early Embryo of the Leech
Asymmetric Cell Divisions in the Early Embryo of the Leech

... Molecular phylogenies have converged on grouping bilaterally symmetric animals into three major clades, a significantly reduced Deuterostomia, and, from the former Protostomia, two new super-phyla called Ecdysozoa and Lophotrochozoa (Aguinaldo et al. 1997; Ruiz-Trillo et al. 1999). Combining this co ...
Contribution of Choline
Contribution of Choline

... Kans.) which was supplemented with choline chloride to a final concentration of 0.02% (CDM-C). Adaptation to ethanolamine (CDM-EA) was done by serially transferring aliquots of pneumococcal cultures growing in CDM in which the choline concentration was decreased in a stepwise manner to a final conce ...
Single gene-based distinction of individual microbial
Single gene-based distinction of individual microbial

... microbes in any given habitat that cannot be detected by conventional culturing strategies. The use of sensitive genetic detection methods such as CARD-FISH and in situ PCR have been limited by the cell wall permeabilization requirement that cannot be performed similarly on all cell types without ly ...
Limbal stem cell deficiency and its management
Limbal stem cell deficiency and its management

... may be necessary to reduce dry eye. Only once eyelid disease and dry eye have been treated effectively should one consider surgical management of limbal stem cell deficiency. The surgical options for limbal stem cell deficiency are based on the transplantation of healthy limbal epithelium. This can ...
A Activity and Augments IL-2 Production Translation Reconstitutes
A Activity and Augments IL-2 Production Translation Reconstitutes

... and HL-1 (BioWhittaker, Walkersville, MD) supplemented with 5% heatinactivated FCS (HyClone, Logan, UT), 25 mM HEPES, 2 mM L-glutamine, 10 ␮g/ml streptomycin, 10 IU/ml penicillin, and 1 ␮g/ml PHA, as described (9). After 2 days, T lymphoblasts were passaged and cultured in the above medium supplemen ...
6 dent antigen specific B cell response
6 dent antigen specific B cell response

... 1. Antigen entry to the secondary lymphatic organs Naive lymphocytes practically encounter the antigens in the secondary lymphoid organs, which provide the proper environment for the extreme proliferation of both types of lymphocytes. For B cells, mostly follicular dendritic cells (FDCs) display the ...
Chapter 3—Cells
Chapter 3—Cells

... All organisms are made of one or more cells. Animal and plant cells contain the same organelles. The cell is the basic unit of living things. All cells originate from other cells. ...
The development of dorsal root ganglia and ventral horns in the
The development of dorsal root ganglia and ventral horns in the

... from 15 days onwards, only large cells of the ventro-lateral columns were counted. In the first place, the diameters of all neuronal nuclei in this area of a selected section were measured at each stage. Where the nuclear membrane was oval in outline, the diameter of a circle of equivalent area was ...
Reproduction of Bacteria
Reproduction of Bacteria

... •Fission is a type of asexual reproduction •Asexual reproduction- reproduction of a living thing from only one parent How?... The one main (circular) chromosome makes a copy of itself Then it divides into two ...
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7-2 Eukaryotic Cell Structure

... Lysosomes are small organelles filled with enzymes. Lysosomes break down lipids, carbohydrates, and proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 23 of 49 Copyright Pearson Prentice Hall ...
ELECTRON-MICROSCOPE ILLUSTRATIONS OF DIVISION IN
ELECTRON-MICROSCOPE ILLUSTRATIONS OF DIVISION IN

... Division of bacteria in man. Very few dividing bacilli were seen, but the morphological changes found were comparable with those seen in material from mice. For example, in an early stage a mesosome was seen in contact with the indented plasma membrane (fig. 18). In a later stage bacterial division ...
The leaf is the fundamental unit of the shoot system, which is
The leaf is the fundamental unit of the shoot system, which is

... floral organs, which are modified leaves. Moreover, leaf shape is sensitive to environmental stimuli. The leaf is therefore the key organ for a full understanding of plant morphogenesis. However, the genetic control of development of these shapes has remained unclear. Recently, studies of leaf morph ...
The Hierarchy of the 3D Genome
The Hierarchy of the 3D Genome

... techniques are all based on formaldehyde crosslinking of chromatin, which creates a genome-wide snapshot of (long-range) interactions between any pair of genomic loci occurring in three dimensions. Chromatin is fragmented, for example by digestion, and then intramolecularly religated so that interac ...
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... from 15 days onwards, only large cells of the ventro-lateral columns were counted. In the first place, the diameters of all neuronal nuclei in this area of a selected section were measured at each stage. Where the nuclear membrane was oval in outline, the diameter of a circle of equivalent area was ...
chapter 1 slides - Mrs. Brenner`s Biology
chapter 1 slides - Mrs. Brenner`s Biology

... periods of time, organisms respond to changing environments by developing new adaptations. • Evolution is the change in a population of organisms over time to become more suited to the environment. ...
Ectopic Expression of Neurogenin 2 Alone is Sufficient to Induce
Ectopic Expression of Neurogenin 2 Alone is Sufficient to Induce

... HEPES (pH 7.3, adjusted with KOH). Currents were recorded with an EPC9 (Heka) patch clamp amplifier and low pass-filtered at 1–2 kHz. Stimulation and data acquisition were controlled by the PULSE/PULSEFIT software package (Heka) on a Macintosh computer, and data analysis was performed with IGOR soft ...
chapter2 467..477 - Caister Academic Press
chapter2 467..477 - Caister Academic Press

... 100 microliter aliquot of washed Micrococcus (above, left side of petri dish) was evenly spread in a thin line across a 0.1X Heart Infusion Broth plus 0.1% glucose plate and allowed to dry. The procedure was repeated with the E. coli strain (right side of petri dish). The cells were allowed to dry f ...
BLM 2-30, Compare the Events of Meiosis and Mitosis Key Meiosis I
BLM 2-30, Compare the Events of Meiosis and Mitosis Key Meiosis I

... separate and are pulled to opposite poles by the spindle fibres ...
Alight-inducible organelle targeting system for dynamically
Alight-inducible organelle targeting system for dynamically

... Pharmacological tools are significantly faster, but many are not quickly reversible due to high binding affinities and the reliance on drug washout (Haruki et al., 2008). In addition, pharmacological approaches lack generality; not all proteins can be easily designed for drug targeting. Compared to ...
VCE_MFST1500-4
VCE_MFST1500-4

... for growth. However, many yeasts will grow in the presence of high sugar or salt concentrations. In general, yeasts require less water than bacteria but more than molds. Foods. Sugars and acids are good food sources for yeasts. Growth is abundant i n food substances containing carbohydrate (sugar or ...
Towards mechanistic models of plant organ growth
Towards mechanistic models of plant organ growth

... molecular level with the entire complexity of plant metabolites, but also at the higher level with cell–cell and organ– organ interactions (Beemster et al., 2003), so the approaches to integrate our experimentally obtained knowledge need to be multi-scale. One approach to start tackling this challen ...
A Piscine Birnavirus Induces Inhibition of Protein Synthesis in CHSE
A Piscine Birnavirus Induces Inhibition of Protein Synthesis in CHSE

... Viruses, as obligatory pathogens, depend on the host machinery to produce their own proteins. They have evolved different strategies of taking advantage of the host machinery of protein synthesis that may or may not be associated with inhibition of protein synthesis. Picorna- and rotaviruses break t ...
A Comparative Analysis of Extra-Embryonic Endoderm Cell
A Comparative Analysis of Extra-Embryonic Endoderm Cell

... populations of primitive endoderm and other lineages since their expression of these markers was more heterogeneous. In particular, the failure of END2 cells to express GATA4 and SOX7, suggests that there are few if any primitive endoderm cells within this line. While they do exhibit heterogeneous e ...
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... In this plant, red flowers are dominant and white flowers are recessive. As well, two leaves are dominant while three leaves is recessive. You do a Test Cross that produces 25 red flowers with three leaves and 25 white flowers with three leaves. What is the genotype of the parent plant with red flow ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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