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... 4% paraformaldehyde in 75 mM Hepes buffer, pH 7.4. They were then rinsed in Hepes buffered saline (HBS; 75 mM Hepes pH 7.4, 130 mM NaC1), treated for 12 hr in 0.2 mg/ml chitinase in HBS at room temperature, and rinsed in buffer. The rest of the hybridization was carried out following the standard pr ...
... 4% paraformaldehyde in 75 mM Hepes buffer, pH 7.4. They were then rinsed in Hepes buffered saline (HBS; 75 mM Hepes pH 7.4, 130 mM NaC1), treated for 12 hr in 0.2 mg/ml chitinase in HBS at room temperature, and rinsed in buffer. The rest of the hybridization was carried out following the standard pr ...
Intracellular distribution of histone mRNAs in human fibroblasts studied
... bimodal population of cells in which =43% of cells exhibited grain densities significantly increased relative to background. Consistent with this result, the fraction of cells in S phase was found to be 44%, as determined by autoradiography of parallel cultures metabolically labeled with [3H]thymidi ...
... bimodal population of cells in which =43% of cells exhibited grain densities significantly increased relative to background. Consistent with this result, the fraction of cells in S phase was found to be 44%, as determined by autoradiography of parallel cultures metabolically labeled with [3H]thymidi ...
organelle in bacillus subtilis
... 10), shows a "chondrio~de" which somewhat resembles the organelles in Figs. 2, 3, 4. The "two unidentified structures" which Tokuyasu and Yamada (22) observed in B. subtilis must also be identical with the organelles described here. The present more general application of the RyterKellenberger techn ...
... 10), shows a "chondrio~de" which somewhat resembles the organelles in Figs. 2, 3, 4. The "two unidentified structures" which Tokuyasu and Yamada (22) observed in B. subtilis must also be identical with the organelles described here. The present more general application of the RyterKellenberger techn ...
7-2 Eukaryotic Cell Structure
... • Sac filled with fluid containing enzymes and acids • Responsible for the digestion of cellular waste and debris ...
... • Sac filled with fluid containing enzymes and acids • Responsible for the digestion of cellular waste and debris ...
How is pluripotency determined and maintained?
... Mitsui et al., 2003). Indeed, Nanog-null ES cells differentiate into Gata6-positive parietal endoderm-like cells, which have a morphology that is similar to that of Gata6-induced cells (Fig. 2) (Mitsui et al., 2003). However, although it has been reported that Nanog expression is partly regulated by ...
... Mitsui et al., 2003). Indeed, Nanog-null ES cells differentiate into Gata6-positive parietal endoderm-like cells, which have a morphology that is similar to that of Gata6-induced cells (Fig. 2) (Mitsui et al., 2003). However, although it has been reported that Nanog expression is partly regulated by ...
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... Mitsui et al., 2003). Indeed, Nanog-null ES cells differentiate into Gata6-positive parietal endoderm-like cells, which have a morphology that is similar to that of Gata6-induced cells (Fig. 2) (Mitsui et al., 2003). However, although it has been reported that Nanog expression is partly regulated by ...
... Mitsui et al., 2003). Indeed, Nanog-null ES cells differentiate into Gata6-positive parietal endoderm-like cells, which have a morphology that is similar to that of Gata6-induced cells (Fig. 2) (Mitsui et al., 2003). However, although it has been reported that Nanog expression is partly regulated by ...
The Aromatic-L-Amino Acid Decarboxylase Inhibitor Carbidopa Is
... Trypan Blue Exclusion Assays. Cells (50,000) were seeded in 60-mm plates (Falcon, Lincoln Park, NJ) in 3 ml of growth medium supplemented with 50 units/ml of penicillin and streptomycin (Life Technologies, Inc.). After incubation overnight, the appropriate AAAD inhibitor concentration or diluent was ...
... Trypan Blue Exclusion Assays. Cells (50,000) were seeded in 60-mm plates (Falcon, Lincoln Park, NJ) in 3 ml of growth medium supplemented with 50 units/ml of penicillin and streptomycin (Life Technologies, Inc.). After incubation overnight, the appropriate AAAD inhibitor concentration or diluent was ...
How Viruses Multiply
... 18. Is the following sentence true or false? Viruses can cause diseases only in humans. ________________________ 19. List at least two ways that viral diseases can be spread. ...
... 18. Is the following sentence true or false? Viruses can cause diseases only in humans. ________________________ 19. List at least two ways that viral diseases can be spread. ...
SASEC2015 Third Southern African Solar Energy Conference 11 – 13 May 2015
... Electroluminescence (EL) is a useful solar cell and photovoltaic module characterisation technique as it is fast, non-destructive and sensitive to the effects of shunt and series resistance and recombination processes. EL is emitted by a solar cell under forward bias, as injected carriers recombine ...
... Electroluminescence (EL) is a useful solar cell and photovoltaic module characterisation technique as it is fast, non-destructive and sensitive to the effects of shunt and series resistance and recombination processes. EL is emitted by a solar cell under forward bias, as injected carriers recombine ...
Redox signaling: hydrogen peroxide as intracellular messenger
... mechanism, the dependence of H2O2 production on the intrinsic tyrosine kinase activity of the EGF receptor and the autophosphorylation sites located in its COOHterminal tail was investigated (Bae et al., 1997). EGF failed to induce H2O2 generation in cells expressing a kinase-inactive EGF receptor. ...
... mechanism, the dependence of H2O2 production on the intrinsic tyrosine kinase activity of the EGF receptor and the autophosphorylation sites located in its COOHterminal tail was investigated (Bae et al., 1997). EGF failed to induce H2O2 generation in cells expressing a kinase-inactive EGF receptor. ...
Parenchyma cells
... Parenchyma – Aerenchyma A lattice cell structure is common in the aerenchyma of several aquatic plants including water hyacinth (Eichhornia) and pickerelweed (Pontederia). This honeycomb effect provides stability and strength with a minimum of structural material and the air spaces provide buoyancy ...
... Parenchyma – Aerenchyma A lattice cell structure is common in the aerenchyma of several aquatic plants including water hyacinth (Eichhornia) and pickerelweed (Pontederia). This honeycomb effect provides stability and strength with a minimum of structural material and the air spaces provide buoyancy ...
The roles of two C. elegans HOX co-factor orthologs
... unc-62(mu232); muIs3[mab-5::lacZ, rol-6(d)], ceh-20(mu290) III; unc-62(mu232) V The positions of the Q.pa daughters were determined directly using Nomarski optics at the end of L1. At this stage, the hypodermal V cells have divided once and the P nuclei have all descended into the cord. The position ...
... unc-62(mu232); muIs3[mab-5::lacZ, rol-6(d)], ceh-20(mu290) III; unc-62(mu232) V The positions of the Q.pa daughters were determined directly using Nomarski optics at the end of L1. At this stage, the hypodermal V cells have divided once and the P nuclei have all descended into the cord. The position ...
Ultrastructural changes in the root tip and leaf cells of Lens culinaris
... acetone solutions of 30%, 50%, 70%, and 90% for 10 min each, 100% acetone for 30 min, 1:1 acetone and propylene oxide for 20 min, and 100% propylene oxide for 20 min. Following dehydration, the samples were infiltrated with 1:1 propylene oxide and epon 812 for 1 h and 1:3 propylene oxide and epon 81 ...
... acetone solutions of 30%, 50%, 70%, and 90% for 10 min each, 100% acetone for 30 min, 1:1 acetone and propylene oxide for 20 min, and 100% propylene oxide for 20 min. Following dehydration, the samples were infiltrated with 1:1 propylene oxide and epon 812 for 1 h and 1:3 propylene oxide and epon 81 ...
here - ScienceA2Z.com
... Foreign genetic material (most commonly DNA) can also be artificially introduced into the cell by a process called transfection. This can be transient, if the DNA is not inserted into the cell's genome, or stable, if it is. Certain viruses also insert their genetic material into the genome. ...
... Foreign genetic material (most commonly DNA) can also be artificially introduced into the cell by a process called transfection. This can be transient, if the DNA is not inserted into the cell's genome, or stable, if it is. Certain viruses also insert their genetic material into the genome. ...
Receptors and immune sensors: the complex entry path of human
... known to induce cell functions associated with progression through the cell cycle and to co-opt several pathways in an ostensible need to optimize the environment for viral replication events. Wang et al. reported that EGFR was cleared from the cell surface after infection – a common consequence of ...
... known to induce cell functions associated with progression through the cell cycle and to co-opt several pathways in an ostensible need to optimize the environment for viral replication events. Wang et al. reported that EGFR was cleared from the cell surface after infection – a common consequence of ...
INTERLEUKIN 6 DECREASES CELL
... carcinoma (34). The characteristic features ofthis cell line have remained constant irrespective of passage history and the cells closely resemble malignant cells in the original effusion (34). As described by Engel et al. (34), ZR75-1 cells are often arranged in rosettes around duct-like lumens and ...
... carcinoma (34). The characteristic features ofthis cell line have remained constant irrespective of passage history and the cells closely resemble malignant cells in the original effusion (34). As described by Engel et al. (34), ZR75-1 cells are often arranged in rosettes around duct-like lumens and ...
Chewing the fat on natural killer T cell development
... CD4+CD8+ double positive (DP) stage of development in the thymus (2). NKT cells are derived from the small fraction of thymocytes that have randomly generated CD1d-reactive T cell receptors (typically comprising Vα14Jα18 combined with either Vβ8.2, Vβ7, or Vβ2). When these cells encounter CD1d molec ...
... CD4+CD8+ double positive (DP) stage of development in the thymus (2). NKT cells are derived from the small fraction of thymocytes that have randomly generated CD1d-reactive T cell receptors (typically comprising Vα14Jα18 combined with either Vβ8.2, Vβ7, or Vβ2). When these cells encounter CD1d molec ...
Epithelial invasion and cell lysis by virulent strains of Streptococcus
... cells and virulence may not always correlate well, the ability to invade HEp-2 cells is strongly indicative of the relative virulence of the strains and clinical outcome of disease following infection [9,10]. Both virulent and avirulent strains of S. suis can be isolated from the tonsils of carrier ...
... cells and virulence may not always correlate well, the ability to invade HEp-2 cells is strongly indicative of the relative virulence of the strains and clinical outcome of disease following infection [9,10]. Both virulent and avirulent strains of S. suis can be isolated from the tonsils of carrier ...
Self-Replication Mechanism by Means of Self
... correct number of cells. After the creation of the complete organism, or when at least two cells ar present in the circuit, a differentiation process is mandatory to let the cells express a different functionality depending on, for instance, their place in the organism (An example of differentiation ...
... correct number of cells. After the creation of the complete organism, or when at least two cells ar present in the circuit, a differentiation process is mandatory to let the cells express a different functionality depending on, for instance, their place in the organism (An example of differentiation ...
Electrochemical model for proton exchange membrane fuel cell
... density is shown in Fig. 4. In Fig. 5 the cell’s power density is shown as a function of current density for two different temperatures. It can be seen from these figures that the fuel cell’s efficiency is low and that a significant part of the theoretical output voltage is lost because of various l ...
... density is shown in Fig. 4. In Fig. 5 the cell’s power density is shown as a function of current density for two different temperatures. It can be seen from these figures that the fuel cell’s efficiency is low and that a significant part of the theoretical output voltage is lost because of various l ...
Permanent cell cycle exit in G2 phase after DNA damage in normal
... MEFs as models. We purposely did not use experimental systems based on tumour-derived cells lines, as many of them have acquired uncharacterized genetic abnormalities, which can confound the interpretation of the data obtained. Moreover, the cell lines we tested exhibited aberrant post-mitotic ®gure ...
... MEFs as models. We purposely did not use experimental systems based on tumour-derived cells lines, as many of them have acquired uncharacterized genetic abnormalities, which can confound the interpretation of the data obtained. Moreover, the cell lines we tested exhibited aberrant post-mitotic ®gure ...
Independent Pathways Viral Activation of Macrophages through
... recombinant gD, also reported to induce IFN-␣ (46, 47), but no induction could be observed (Fig. 2B). Together these data clearly show that the induction pathway for IFN-␣ during HSV infections in macrophages does not rely on interactions between viral glycoproteins and cell surface receptors, but ...
... recombinant gD, also reported to induce IFN-␣ (46, 47), but no induction could be observed (Fig. 2B). Together these data clearly show that the induction pathway for IFN-␣ during HSV infections in macrophages does not rely on interactions between viral glycoproteins and cell surface receptors, but ...
Cytoplasmic calcium measurements in intact higher plant cells
... demonstrate that cytoplasmic calcium levels in plant cells do change when signals are received (Hepler & Wayne, 1985; Clarkson, 1986). The first report that this does occur showed that, when Nitellopsis cells are transferred from darkness to light, the cytoplasmic calcium ion activity, measured by ...
... demonstrate that cytoplasmic calcium levels in plant cells do change when signals are received (Hepler & Wayne, 1985; Clarkson, 1986). The first report that this does occur showed that, when Nitellopsis cells are transferred from darkness to light, the cytoplasmic calcium ion activity, measured by ...
Slides - Institute for Evidence
... Bioremediation: the concept Microbes, like all life, need an ecological niche. Some get it by brawn (growing very fast)… …some by brain (living off material that others can't). Any abundant, energy-rich organic material that is hard to degrade thus provides selective pressure to evolve the machiner ...
... Bioremediation: the concept Microbes, like all life, need an ecological niche. Some get it by brawn (growing very fast)… …some by brain (living off material that others can't). Any abundant, energy-rich organic material that is hard to degrade thus provides selective pressure to evolve the machiner ...
The Plant Extracellular Matrix: News from the Cell`s Frontier
... flotation centrifugation, and a sequencedependent glucanase was used to show that the synthase produced the expected products, cellotriosyl and cellotetraosyl unit structures, in vitro (Gibeaut and Carpita, 1994). However, a callose synthase activity was observed in the maize Golgi membranes in addi ...
... flotation centrifugation, and a sequencedependent glucanase was used to show that the synthase produced the expected products, cellotriosyl and cellotetraosyl unit structures, in vitro (Gibeaut and Carpita, 1994). However, a callose synthase activity was observed in the maize Golgi membranes in addi ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.