Non-Flagellar Swimming in Marine Synechococcus
... medium viscosity (Willey, 1988). Cells that become fortuitously attached to the slide or coverslip will rotate about their point of attachment at an average rate of 1 revolution/sec. Cells can rotate either in the clockwise (CW) or the counterclockwise (CCW) direction but a rotating cell has never b ...
... medium viscosity (Willey, 1988). Cells that become fortuitously attached to the slide or coverslip will rotate about their point of attachment at an average rate of 1 revolution/sec. Cells can rotate either in the clockwise (CW) or the counterclockwise (CCW) direction but a rotating cell has never b ...
In Vitro Toxicology and Cellular Fate Determination Using
... Beckman Coulter's Biomek® 2000 Laboratory Automation Workstation is a medium-throughput automation platform capable of performing these assays through liquid handling, plate movement and integrated shaking. For staurosporine-treated cultures, all three assay types were set up on the Biomek® 2000 wor ...
... Beckman Coulter's Biomek® 2000 Laboratory Automation Workstation is a medium-throughput automation platform capable of performing these assays through liquid handling, plate movement and integrated shaking. For staurosporine-treated cultures, all three assay types were set up on the Biomek® 2000 wor ...
Viruses and Viral Like Agents
... • About 5, 000 viruses have been identified, but there is believed to be around 400, 000 different viruses. • Believed to be the most abundant biological entity on Earth!! • Viruses are very small- they do not “grow,” just reproduce • They cannot reproduce on their own (obligate, intracellular parti ...
... • About 5, 000 viruses have been identified, but there is believed to be around 400, 000 different viruses. • Believed to be the most abundant biological entity on Earth!! • Viruses are very small- they do not “grow,” just reproduce • They cannot reproduce on their own (obligate, intracellular parti ...
Left Ventricular Assist Device (LVAD)
... »» Adhesion glycoproteins »» Glycosaminoglycans (GAGs) »» Proteoglycans »» Matricellular proteins CorMatrix is produced in a manner that retains these natural ECM molecules including growth factors, proteins, and cytokines which are known to play important roles in host tissue repair and remodeling. ...
... »» Adhesion glycoproteins »» Glycosaminoglycans (GAGs) »» Proteoglycans »» Matricellular proteins CorMatrix is produced in a manner that retains these natural ECM molecules including growth factors, proteins, and cytokines which are known to play important roles in host tissue repair and remodeling. ...
Clostridium sordellii Lethal-Toxin Autoprocessing and
... DxD supports previous research showing that glucosylation of the host cell GTPases leads to arrest of the cell cycle and induction of apoptosis (28). The decrease in cytotoxicity seen with TcsL C698A suggests that the autoprocessing activity is important in TcsL cytotoxicity (Fig. 2). Previous studi ...
... DxD supports previous research showing that glucosylation of the host cell GTPases leads to arrest of the cell cycle and induction of apoptosis (28). The decrease in cytotoxicity seen with TcsL C698A suggests that the autoprocessing activity is important in TcsL cytotoxicity (Fig. 2). Previous studi ...
Basement membrane matrices in mouse embryogenesis
... in early development and teratocarcinoma differentiation. In the early embryo a compartmentalization of newly formed cell types takes place immediately by formation of basement membranes The stage-specific developmental appearance of extracellular matrix molecules such as type IV collagen, laminin. ...
... in early development and teratocarcinoma differentiation. In the early embryo a compartmentalization of newly formed cell types takes place immediately by formation of basement membranes The stage-specific developmental appearance of extracellular matrix molecules such as type IV collagen, laminin. ...
The chemokine SDF1/CXCL12 and its receptor CXCR4 regulate
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
L-Carnosine reduces telomere damage and shortening
... obtained at passage eight from the Cell Bank of the Chinese Medical Academic Science (Beijing, China). Cells were grown in DMEM supplemented with 10% fetal bovine serum and 100 U/ml penicillin/ streptomycin sulfate at 37 C with 5% CO2 and 95% air. Serial cultivation was carried out with a split rat ...
... obtained at passage eight from the Cell Bank of the Chinese Medical Academic Science (Beijing, China). Cells were grown in DMEM supplemented with 10% fetal bovine serum and 100 U/ml penicillin/ streptomycin sulfate at 37 C with 5% CO2 and 95% air. Serial cultivation was carried out with a split rat ...
The Rice TAL Effector–Dependent Resistance
... EBEAvrXa27 probes, but not by nonspecific probes (Supplemental Figure 7D). Specific interactions between AvrXa10 and EBEAvrXa10 and between AvrXa27 and EBEAvrXa27 were also observed in yeast using a one-hybrid assay (Supplemental Figures 8A to 8C). XA10 Triggers an HR in Rice and N. benthamiana Initia ...
... EBEAvrXa27 probes, but not by nonspecific probes (Supplemental Figure 7D). Specific interactions between AvrXa10 and EBEAvrXa10 and between AvrXa27 and EBEAvrXa27 were also observed in yeast using a one-hybrid assay (Supplemental Figures 8A to 8C). XA10 Triggers an HR in Rice and N. benthamiana Initia ...
Yeast Hansenula wingei
... involved in reproduction, In particular, there has been no analysis of the physiological processes involved in cell fusion, the first step in the mating cycle in fungi and most other organisms. As Hawker emphasized, the higher fungi are not suitable for such an analysis, partly because they are diff ...
... involved in reproduction, In particular, there has been no analysis of the physiological processes involved in cell fusion, the first step in the mating cycle in fungi and most other organisms. As Hawker emphasized, the higher fungi are not suitable for such an analysis, partly because they are diff ...
chlamydomonas gymnogama and - The Journal of Cell Biology
... presumed to be wall precursor, was observed in C. for at least 32% of the weight of the cell wall. The alkaline hydrolysis treatment which releases the reinhardtii (6). Evidence is also beginning to accumulate that carbohydrate from the plas- hydroxyproline oligosaccharides shown in Fig. 6 mamembran ...
... presumed to be wall precursor, was observed in C. for at least 32% of the weight of the cell wall. The alkaline hydrolysis treatment which releases the reinhardtii (6). Evidence is also beginning to accumulate that carbohydrate from the plas- hydroxyproline oligosaccharides shown in Fig. 6 mamembran ...
Extracellular matrix stiffness in regulation of intestinal stem cell
... Stem cells have the significant ability to divide interminably and to generate all the specialized cell types in the body. Characteristics, such as being undifferentiated and having the ability to self-renew and to generate daughter cells with distinct cell fates, separate stem cells from other cell ...
... Stem cells have the significant ability to divide interminably and to generate all the specialized cell types in the body. Characteristics, such as being undifferentiated and having the ability to self-renew and to generate daughter cells with distinct cell fates, separate stem cells from other cell ...
Role of plectin in cytoskeleton organization and dynamics
... domains which probably form looplike structures exposed to the outside of globular protein core structures. There is evidence from both in vitro and in vivo experiments indicating that plectin-IF interactions are differentially regulated by phosphorylation involving different types of protein kinase ...
... domains which probably form looplike structures exposed to the outside of globular protein core structures. There is evidence from both in vitro and in vivo experiments indicating that plectin-IF interactions are differentially regulated by phosphorylation involving different types of protein kinase ...
topic #4: angiosperm anatomy and selected aspects
... auxiliary mechanism operative in some plants at some times. Although the cohesion theory has come under attack periodically,8 supporters rally and provide counterbalancing evidence.) An understanding of sap ascent will come more easily if you first reflect on how a plant is constructed. Individual c ...
... auxiliary mechanism operative in some plants at some times. Although the cohesion theory has come under attack periodically,8 supporters rally and provide counterbalancing evidence.) An understanding of sap ascent will come more easily if you first reflect on how a plant is constructed. Individual c ...
human embryonic stem cells and their clinical relevance
... are quickly depleted as they differentiate to other cell types with more limited developmental potential. However, if the inner cell mass cell is removed from its normal embryonic environment and cultured under appropriate conditions, these cells can continue to proliferate and replicate themselves ...
... are quickly depleted as they differentiate to other cell types with more limited developmental potential. However, if the inner cell mass cell is removed from its normal embryonic environment and cultured under appropriate conditions, these cells can continue to proliferate and replicate themselves ...
chlamydomonas gymnogama and
... presumed to be wall precursor, was observed in C. for at least 32% of the weight of the cell wall. The alkaline hydrolysis treatment which releases the reinhardtii (6). Evidence is also beginning to accumulate that carbohydrate from the plas- hydroxyproline oligosaccharides shown in Fig. 6 mamembran ...
... presumed to be wall precursor, was observed in C. for at least 32% of the weight of the cell wall. The alkaline hydrolysis treatment which releases the reinhardtii (6). Evidence is also beginning to accumulate that carbohydrate from the plas- hydroxyproline oligosaccharides shown in Fig. 6 mamembran ...
A matter of size: developmental control of organ size in plants
... Arabidopsis thaliana plants grown under the same conditions are remarkably uniform in size. In contrast, the flowers and petals of Brassica napus, another member of Brassicaceae, are always much larger than those of Arabidopsis (Figure 1). Although it has long been established that organ size within ...
... Arabidopsis thaliana plants grown under the same conditions are remarkably uniform in size. In contrast, the flowers and petals of Brassica napus, another member of Brassicaceae, are always much larger than those of Arabidopsis (Figure 1). Although it has long been established that organ size within ...
DNA Methylation Profiles Define Stem Cell Identity and
... profiles identify a cohort of developmentally regulated sequence elements, such as orphan CpG islands, that will be most valuable to uncover novel transcriptional regulators and pivotal ‘‘gatekeeper’’ genes in pluripotency and lineage differentiation. STEM CELLS 2012;30:2732–2745 ...
... profiles identify a cohort of developmentally regulated sequence elements, such as orphan CpG islands, that will be most valuable to uncover novel transcriptional regulators and pivotal ‘‘gatekeeper’’ genes in pluripotency and lineage differentiation. STEM CELLS 2012;30:2732–2745 ...
Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan
... on the inhibition of plant cysteine synthase by D-cycloserine, the activity of Salmonella cysteine synthase is decreased (Nakamura et al. 1984). Although these enzymes are involved in metabolic pathways in chloroplasts, the relationship between changes in their enzymatic activities and chloroplast d ...
... on the inhibition of plant cysteine synthase by D-cycloserine, the activity of Salmonella cysteine synthase is decreased (Nakamura et al. 1984). Although these enzymes are involved in metabolic pathways in chloroplasts, the relationship between changes in their enzymatic activities and chloroplast d ...
Dynamic Clamp
... This inputs reduces input resistance and time constants by 80% nad provide a huge depolarization. Dynamic clamp is used to provide in vivo like conditions in vitro by applying high rates of artificial inputs. ...
... This inputs reduces input resistance and time constants by 80% nad provide a huge depolarization. Dynamic clamp is used to provide in vivo like conditions in vitro by applying high rates of artificial inputs. ...
Entry of oomycete and fungal effectors into plant and animal host cells
... cytoplasm. Recognition of effector proteins by NB-LRR proteins triggers very vigorous and effective defence responses called effector-triggered immunity (Jones and Dangl, 2006). Because effector-triggered immunity can provide a highly effective defence, plant genes encoding NB-LRR proteins that reco ...
... cytoplasm. Recognition of effector proteins by NB-LRR proteins triggers very vigorous and effective defence responses called effector-triggered immunity (Jones and Dangl, 2006). Because effector-triggered immunity can provide a highly effective defence, plant genes encoding NB-LRR proteins that reco ...
Signal Requirements for the Generation of CD4+ and CD8+ T
... were nonspecifically triggering lytic mechanisms by the effector cells and whether EC alloantigens were actually involved in the generation of the response, multiple genetically unrelated EC lines were used as targets in the final cytotoxicity assay, in addition to the NKsensitive line, K562. The re ...
... were nonspecifically triggering lytic mechanisms by the effector cells and whether EC alloantigens were actually involved in the generation of the response, multiple genetically unrelated EC lines were used as targets in the final cytotoxicity assay, in addition to the NKsensitive line, K562. The re ...
Asymmetric Cell Divisions in the Early Embryo of the Leech
... Molecular phylogenies have converged on grouping bilaterally symmetric animals into three major clades, a significantly reduced Deuterostomia, and, from the former Protostomia, two new super-phyla called Ecdysozoa and Lophotrochozoa (Aguinaldo et al. 1997; Ruiz-Trillo et al. 1999). Combining this co ...
... Molecular phylogenies have converged on grouping bilaterally symmetric animals into three major clades, a significantly reduced Deuterostomia, and, from the former Protostomia, two new super-phyla called Ecdysozoa and Lophotrochozoa (Aguinaldo et al. 1997; Ruiz-Trillo et al. 1999). Combining this co ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.