Assays for Cell Enumeration, Cell Proliferation and Cell Cycle
... corresponding ribonucleotide (BrUTP, B21551) that has been microinjected into cells is incorporated into RNA of a nucleolar compartment, a process that should also be detectable with fluorescent anti-BrdU conjugates. ABSOLUTE-S SBIP Cell Proliferation Assay Kit Many conventional BrdU-based protocols ...
... corresponding ribonucleotide (BrUTP, B21551) that has been microinjected into cells is incorporated into RNA of a nucleolar compartment, a process that should also be detectable with fluorescent anti-BrdU conjugates. ABSOLUTE-S SBIP Cell Proliferation Assay Kit Many conventional BrdU-based protocols ...
Primordial germ cell migration - The International Journal of
... et al., 2002, Tanaka and Matsui, 2002). Stella is a novel gene with no known function. Fragilis is a member of an interferon inducible family of genes Fig. 1. Germ cell migration in the mouse. The behavior of PGCs can be divided into six stages based on implicated in homotypic cell-cell adhetime lap ...
... et al., 2002, Tanaka and Matsui, 2002). Stella is a novel gene with no known function. Fragilis is a member of an interferon inducible family of genes Fig. 1. Germ cell migration in the mouse. The behavior of PGCs can be divided into six stages based on implicated in homotypic cell-cell adhetime lap ...
MES-1 and asymmetric embryo divisions
... Asymmetric localization of cytoplasmic factors and unequal cell division are fundamental to the development of all eukaryotes. When these mechanisms are used to generate distinct daughter cells during development, the two events must be coordinately regulated, to ensure the proper segregation of fac ...
... Asymmetric localization of cytoplasmic factors and unequal cell division are fundamental to the development of all eukaryotes. When these mechanisms are used to generate distinct daughter cells during development, the two events must be coordinately regulated, to ensure the proper segregation of fac ...
The differentiation in vitro of the neural crest cells of the
... In the present study, culture of trunk neural tube of mice gave rise to the appearance of a population of small stellate cells. Judging by their characteristic morphology, it seems that these cells are neural crest cells (Cohen & Konigsberg, 1975; Maxwell, 1976). In contrast to quail neural crest, d ...
... In the present study, culture of trunk neural tube of mice gave rise to the appearance of a population of small stellate cells. Judging by their characteristic morphology, it seems that these cells are neural crest cells (Cohen & Konigsberg, 1975; Maxwell, 1976). In contrast to quail neural crest, d ...
Endoplasmic reticulum stress-induced programmed cell death in
... and 10 µg/ml kanamycin. Cells were subcultured every 3 weeks by making a 1:10 dilution in 40 ml of fresh medium and maintained at 24°C with a 16-hour photoperiod with constant shaking. Treatments with CPA were performed 3 weeks after reinoculation, during the exponential growth phase of the cells. S ...
... and 10 µg/ml kanamycin. Cells were subcultured every 3 weeks by making a 1:10 dilution in 40 ml of fresh medium and maintained at 24°C with a 16-hour photoperiod with constant shaking. Treatments with CPA were performed 3 weeks after reinoculation, during the exponential growth phase of the cells. S ...
Extracellular Electrical Fields Direct Wound Healing and Regeneration
... interest has been renewed for understanding (1) the physiological basis of the injury currents, (2) their role in development, regeneration, and repair, (3) optimization of applied EFs in the clinic to promote healing and regeneration, and (4) the mechanisms by which cells sense these weak EFs and c ...
... interest has been renewed for understanding (1) the physiological basis of the injury currents, (2) their role in development, regeneration, and repair, (3) optimization of applied EFs in the clinic to promote healing and regeneration, and (4) the mechanisms by which cells sense these weak EFs and c ...
LIFEPAC® 10th Grade Science Unit 8 Worktext - HomeSchool
... possessing chromosomes is mitosis, but there is another type of cell division. It is meiosis. Meiosis, a nuclear and cell division process, is highly limited in cell type and time of occurrence. In contrast to mitosis, meiosis is a reduction division. The daughter cells produced in a reduction divis ...
... possessing chromosomes is mitosis, but there is another type of cell division. It is meiosis. Meiosis, a nuclear and cell division process, is highly limited in cell type and time of occurrence. In contrast to mitosis, meiosis is a reduction division. The daughter cells produced in a reduction divis ...
A.G. Scientific, Inc. Protease Inhibitor Cocktail VI, Plant Cells
... Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and ...
... Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and ...
as a PDF
... which are plant pathogens are often highly specialized and are pathogenic on only one or a few species of plants. Most "host—specific" pathogen species have a number of races, each of which is distinct from the others in its ability to attack various varieties (cultivars) of its host plant species. ...
... which are plant pathogens are often highly specialized and are pathogenic on only one or a few species of plants. Most "host—specific" pathogen species have a number of races, each of which is distinct from the others in its ability to attack various varieties (cultivars) of its host plant species. ...
Adlai E. Stevenson High School Course Description
... Compare and contrast mitosis and meiosis. (Cellular Reproduction) Discuss cellular structure and function, including membrane structure and transport. (Cells) Discuss Mendel’s Laws of Inheritance and other selected topics in the area of Genetics and apply knowledge of Genetics to solve problems invo ...
... Compare and contrast mitosis and meiosis. (Cellular Reproduction) Discuss cellular structure and function, including membrane structure and transport. (Cells) Discuss Mendel’s Laws of Inheritance and other selected topics in the area of Genetics and apply knowledge of Genetics to solve problems invo ...
Inhibition of Hepatitis C Virus Genotype 1a Non
... (hepatic cirrhosis, fibrosis and hepatocellular carcinoma) are significant health concerns in developing world and even in developed countries [1]. HCV infection results in more than 350,000 deaths every year predominantly due to hepatic cirrhosis and hepatocellular carcinoma [2]. HCV is a small, en ...
... (hepatic cirrhosis, fibrosis and hepatocellular carcinoma) are significant health concerns in developing world and even in developed countries [1]. HCV infection results in more than 350,000 deaths every year predominantly due to hepatic cirrhosis and hepatocellular carcinoma [2]. HCV is a small, en ...
Cancer Prevention Strategies That Address the Evolutionary
... years of simulated time), and late (24,000 time steps 33 years of simulated time) application of the therapies relative to the initiation of the neoplasm. This approximates the time scale of progression in human neoplasms (45-47). The probability of the simulated neoplastic tissue developing HGD i ...
... years of simulated time), and late (24,000 time steps 33 years of simulated time) application of the therapies relative to the initiation of the neoplasm. This approximates the time scale of progression in human neoplasms (45-47). The probability of the simulated neoplastic tissue developing HGD i ...
D. Growth and Reproduction
... Fun Facts about Bacteria 1. Bacteria help our bodies with digestion and produce needed vitamins. 2. Bacteria help us by destroying harmful organisms within our bodies. 3. There are more bacterial cells in your body than there are human cells. 4. Bacteria are used to make cheese, milk, sourdough bre ...
... Fun Facts about Bacteria 1. Bacteria help our bodies with digestion and produce needed vitamins. 2. Bacteria help us by destroying harmful organisms within our bodies. 3. There are more bacterial cells in your body than there are human cells. 4. Bacteria are used to make cheese, milk, sourdough bre ...
Membrane Remodeling and Organization: Elements Common to
... Flotillins are elements conserved among vertebrates and invertebrates, and they are ubiquitous. They belong to a family of proteins that contain a conserved SPFH (Stomatin, Prohibitin, Flotillin, HlfK/C) N-terminal domain and a C-terminal domain required for their oligomerization (29). Flotillin hom ...
... Flotillins are elements conserved among vertebrates and invertebrates, and they are ubiquitous. They belong to a family of proteins that contain a conserved SPFH (Stomatin, Prohibitin, Flotillin, HlfK/C) N-terminal domain and a C-terminal domain required for their oligomerization (29). Flotillin hom ...
Recent Advances in αβ T Cell Biology: Wnt Signaling
... Wnt proteins and related signal transduction have been known to influence lymphocyte development [14]. T cells are generated in the thymus, and thymic epithelium is the main source of Wnt proteins [15,16]. Expression of ecto-domains of Frizzled receptors by retroviral transduction as extracellular i ...
... Wnt proteins and related signal transduction have been known to influence lymphocyte development [14]. T cells are generated in the thymus, and thymic epithelium is the main source of Wnt proteins [15,16]. Expression of ecto-domains of Frizzled receptors by retroviral transduction as extracellular i ...
Character and origin of vacuoles induced in mammalian cells by the
... addition of toxigenic filtrate. LY was applied at a final concentration of 0.5 mg/ml in PBS and FITC-dextran at a final concentration of 1 mg/ml in PBS. The cells were incubated for 30 min-22 h as described above, rinsed four times with PBS, mounted on microscopes Materials and methods slides at 25 ...
... addition of toxigenic filtrate. LY was applied at a final concentration of 0.5 mg/ml in PBS and FITC-dextran at a final concentration of 1 mg/ml in PBS. The cells were incubated for 30 min-22 h as described above, rinsed four times with PBS, mounted on microscopes Materials and methods slides at 25 ...
Cell Receptor To Promote B Cell Survival R Interacts and
... cooperate to allow B cell survival at multiple stages of peripheral B cell differentiation and during immune responses. BCR promotes BAFFR-mediated signals through at least two mechanisms by upregulating the expression of BAFFR and by supplying the noncanonical NF-kB pathway substrate p100 for BAFFR ...
... cooperate to allow B cell survival at multiple stages of peripheral B cell differentiation and during immune responses. BCR promotes BAFFR-mediated signals through at least two mechanisms by upregulating the expression of BAFFR and by supplying the noncanonical NF-kB pathway substrate p100 for BAFFR ...
Nitrogen Metabolism in Plant Cell Suspension Cultures
... (1, 7), or by glutamate synthase (5, 9). We suggest that the role of organic acids is to supply a-ketoglutarate, which is a substrate for these enzymes. Withdrawal of a-ketoglutarate from the Krebs' cycle would stop further operation of this cycle unless another intermediate is replaced. This replac ...
... (1, 7), or by glutamate synthase (5, 9). We suggest that the role of organic acids is to supply a-ketoglutarate, which is a substrate for these enzymes. Withdrawal of a-ketoglutarate from the Krebs' cycle would stop further operation of this cycle unless another intermediate is replaced. This replac ...
Bio 103 Lecture - Origin and Evolution of Micr
... • What were the characteristics of the atmosphere of early earth? • How much oxygen was present in the early earth's atmosphere, how much is present today and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what wa ...
... • What were the characteristics of the atmosphere of early earth? • How much oxygen was present in the early earth's atmosphere, how much is present today and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what wa ...
Identification of a nuclear-localized nuclease from wheat cells
... in plants are poorly known. In tracheary elements, a nuclease, ZEN1, has been shown to degrade nuclear DNA without forming the ladder characteristic of internucleosomal fragmentation [30]. In contrast, dying aleurone cells display internucleosomal fragmentation of DNA, which is associated with the p ...
... in plants are poorly known. In tracheary elements, a nuclease, ZEN1, has been shown to degrade nuclear DNA without forming the ladder characteristic of internucleosomal fragmentation [30]. In contrast, dying aleurone cells display internucleosomal fragmentation of DNA, which is associated with the p ...
Caspase Colorimetric Protease Assay Sampler Kit (Caspases-2,
... substrates by caspases, absorption of light by free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase activity. ...
... substrates by caspases, absorption of light by free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase activity. ...
The origins of multicellular organisms
... alignment‐of‐fitness phase in which genetic similarity among cells prevents cell–cell conflict and an export‐of‐fitness phase in which cells become interdependent and collaborate in a sustained effort (reviewed by Folse and Roughgarden 2012). The first phase can be achieved by any “unicellular bottlenec ...
... alignment‐of‐fitness phase in which genetic similarity among cells prevents cell–cell conflict and an export‐of‐fitness phase in which cells become interdependent and collaborate in a sustained effort (reviewed by Folse and Roughgarden 2012). The first phase can be achieved by any “unicellular bottlenec ...
Regulation by Polyamines of Ornithine
... Polyamines are required for cell growth and cell division in eukaryotic and prokaryotic organisms. In the unicellular green alga Chlamydomonas reinhardtii, biosynthesis of the commonly occurring polyamines (putrescine, spermidine, and spermine) is dependent on the activity of ornithine decarboxylase ...
... Polyamines are required for cell growth and cell division in eukaryotic and prokaryotic organisms. In the unicellular green alga Chlamydomonas reinhardtii, biosynthesis of the commonly occurring polyamines (putrescine, spermidine, and spermine) is dependent on the activity of ornithine decarboxylase ...
ANTIIDIOTYPIC IMMUNITY IN INTERSTITIAL NEPHRITIS II. Rats
... in one footpad with 50 ~g of soluble antigen (either BN52/19, RE-Id, BN48/12, control Ig, HGG, SRTA, and SLA, depending on the experiment) in 50 t~l of PBS. Swelling as an index of DTH was measured 24 h later using a spring-loaded engineer's micrometer (Schlesingers for Tools Ltd., Brooklyn, NY). Th ...
... in one footpad with 50 ~g of soluble antigen (either BN52/19, RE-Id, BN48/12, control Ig, HGG, SRTA, and SLA, depending on the experiment) in 50 t~l of PBS. Swelling as an index of DTH was measured 24 h later using a spring-loaded engineer's micrometer (Schlesingers for Tools Ltd., Brooklyn, NY). Th ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.