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Bacteriology Study of ___________ BACTERIA!!! OMG they are everywhere What do you know about bacteria? Fun Facts about Bacteria 1. Bacteria help our bodies with digestion and produce needed vitamins. 2. Bacteria help us by destroying harmful organisms within our bodies. 3. There are more bacterial cells in your body than there are human cells. 4. Bacteria are used to make cheese, milk, sourdough bread & yogurt. 5. 99% of all bacteria are helpful. 6. Dead or weakened bacteria & viruses are used for making helpful vaccines. 7. Scientists estimate that bacteria produce nearly half the oxygen found in the atmosphere. 8. Helpful bacteria are used to purify water at sewage treatment plants and to break down oil after oil spills. 9. One healthy bacterium could reproduce into a colony of more than 2 million in just seven hours. 10. There are more microbes on your body than there are humans on the entire planet. 11. An area of skin as small as 6.5 square cm (1 square inch) may be home to more than 500,000 microbes. Bacteria Evolution 5 • Bacteria fossils have been found in rocks from the Devonian Period (416 to 359.2 mya) • Fossil evidence suggests that bacteria have been on Earth at least 3.5 billion years • During the Proterozoic Eon (~1.5 bya) oxygen appeared in the atmosphere as a results of the action of the cyanobacteria Where do Bacteria live? • • • • • • • 4 Soil Acidic Hot Springs Radioactive Waste Water Deep in the Earth's Crust Outer Space Plants & Animals Classification of Bacteria Domains • Archae o Kingdom: Archaebacteria • Bacteria o Kingdom: Eubacteria 6 How to study and identify A. Morphology: 1.The branch of biology dealing with the form and structure of organisms. 2.The form and structure of an organism considered as a whole. B. Cell Structures: 1. Intracellular: Inside the cell 2. Extracellular: outside the cell C. Metabolism: Gathering and using energy D. Growth and Reproduction: Multiply Hypothesis: Sketch of Bacteria/ Human Cells Bacteria Human Size of Bacteria Power of ten A. Morphology B. Cell Structures Which organelles are you familiar with? Extracellular 7 What are the functions of the following? • • • • • Capsule: Cell Wall: Pili: Flagella: Cytoplasmic Membrane: Intracellular 7 What are the functions of the following? • Nucleoid: • Ribosomes: • Cytoplasm: Different Types of Bacteria Cell Walls 3 Extracellular Structures Bacteria Metabolism 8 What nutrients do bacteria want? • C • N • O (Sugar, Lipid) => Energy, Biosynthesis (Protein) => Biosynthesis (Air) => Energy Bacteria Process these nutrient through: • Aerobic Respiration • Anaerobic Respiration • Anaerobic Fermentation C. E. coli Metabolism Where does this take place? Bacteria Reproduction: Many ways to make more D. Growth and Reproduction Reproduction Binary fission Describe what you see..... Colony Morphology Making bacteria work Links 1. Virtual Museum of Bacteria: http://www.bacteriamuseum.org/niches/features/morphology.shtml 2. Image of Bacteriology http://hospital.blood.co.uk/diagnostic_services/transfusion_microbiology_services/ final_images/BacWork.jpg 3. Image of Gram Positive/ Gram Negative http://gsbs.utmb.edu/microbook/images/fig2_6.jpg 4. Where to find bacteria http://en.wikipedia.org/wiki/Bacteria 5. Bacteria Evolution http://www.britannica.com/EBchecked/topic/48203/bacteria/272371/Evo lution-of-bacteria 6. Bacteria Classification http://www.biologycorner.com/bio1/notes_domains_kingdoms.gif 7. Intracellular Components http://micro.magnet.fsu.edu/cells/bacteriacell.html 8. Bacteria Metabolism http://www.northern.wvnet.edu/~tdanford/slide2.htm How to use a Microscope 1.Start on Low Power 2.Use large focus to find the image on the slide 3.Use fine focus to make the image clearer 4.Move to Medium Power 5.Use fine focus to make the image clearer 6.Move to High Power 7.Use fine focus to make the image clearer Human Cheek Cell 1. Use a toothpick to scrape the inside of your check to collect cheek cells. 2. Place the cells onto a microscope slide 3. Place dye on to your cheek cells 4. Cover the sample with a cover slip 5. Look at your cells under the microscope on low power & sketch 6. Look at your cells under the microscope on medium power & sketch 7. Look at your cells under the microscope on high power & sketch Bacteria Cells