Download Antibody to bcl-2 Oncoprotein

Antibody to bcl-2
Oncoprotein
Catalogue No: VP-B201
Product Specifications
Intended use: For in-vitro diagnostic use.
Mouse Monoclonal Anti-Human bcl-2 oncoprotein is intended for
immunohistochemistry methodologies. A differential diagnosis is
complemented by using a panel of antibodies and in conjunction
with morphological studies, patient clinical history and other
diagnostic tests by a qualified pathologist.
A translocation occurs in a high percentage of follicular
lymphomas which positions the bcl-2 against immunoglobulin
heavy chain gene, with resulting overexpression of bcl-2. Bcl-2 is
expressed as a 26kD protein that lies within the cell and is
thought to act as an inhibitor of apoptosis. Bcl-2 expression is,
therefore, inhibited in germinal centres where apoptosis forms
part of the B cell production pathway.
Storage and stability: Store at 2-8° C. Do not freeze. Do not use
after expiration date on vial.
Specificity: Human bcl-2 oncoprotein.
Clone:
bcl-2/100/D5
Ig Class: IgG1
Mouse IgG conc: See details on vial label
Immunogen: Synthetic peptide sequence
(GAAPAPGIFSSQPGC-COOH).
Product: 1ml liquid tissue culture supernatant containing 15mM
sodium azide.
Precautions: As this product is derived from biological material
proper handling procedures should be employed. Sodium azide
is not considered hazardous at 15mM. However, an MSDS is
available on request or online (www.vectorlabs.com) for safety or
disposal information.
Specimen Preparation
Paraffin sections: VP-B201 can be used on paraffin wax
embedded tissue fixed in 10% neutral buffered formalin. Pretreatment of tissue is essential using a high temperature antigen
unmasking technique. Optimal results can be achieved with
Vector Antigen Unmasking solution (H-3300).
Frozen sections: Yes.
INSTRUCTIONS FOR USE
Dilutions and times are only a guide, there may be variations due
to fixation, specimen collection and preparation, optimal
conditions should be established by the individual laboratory.
Following antigen unmasking of paraffin wax embedded tissue
VP-B201 should be applied to sections in the dilution range 1:80
o
for 60 minutes at 25 C.
Positive control tissue: A positive control should be used in
each run. The recommended control tissue is tonsil.
Negative control tissue: A negative control tissue should be set
up to verify the specificity of the antibody. The recommended
negative control is cerebellum.
Negative control reagent: A negative control mouse
immunoglobulin (e.g. I-2000) should be used to evaluate nonspecific binding.
Visualisation:
®
VECTASTAIN Elite ABC Peroxidase Kits (PK-6200 or PK-6102,
PK-7200) in conjunction with substrate kits (SK-4100 or SK4800) are all recommended for use with this antibody. Specific
protocols are included in each kit.
For professional use only.
Selected References:
von Haefen C, Wieder T, Gillissen B et al. (2002) Ceramide induces mitochondrial activation and apoptosis via a Bax-dependent pathway
in human carcinoma cells. Oncogene 21(25):4009.
Takes RP, Baatenburg de Jong RJ, Wijffels K et al. (2001) Expression of genetic markers in lymph node metastases compared with their
primary tumours in head and neck cancer. Journal of Pathology 194:298.
LeBrun D P, Kamel O W, Cleary M L, et al. (1992) Follicular lymphomas of the gastrointestinal tract. Pathologic features in 31 cases and
bcl-2 oncogenic protein expression. American Journal of Pathology 140 :1327.
Liu Y J, Mason D Y, Johnson G D, et al. (1991) Germinal centre cells express bcl-2 protein after activation by signals which prevent their
entry into apoptosis. European Journal of Immunology 21 :1905.
Hockenbery D, Nunez G, Milliman C, et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.
Nature 348 :334.
Pezella F, Tse A G D, Cordell J L, et al. (1990) Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal
translocation. American Journal of Pathology 137 :225.
Authorised representative: Vector Laboratories Ltd
3 Accent Park
Bakewell Road
Orton Southgate
Peterborough
PE2 6XS, U.K.
Tel: 01733 237999 • Fax: 01733 237119
Vector Laboratories Inc.• 30 Ingold Road • Burlingame • CA 94010 USA
Tel: (650) 697-3600 • Fax: (650) 697-0339
Email: [email protected] • Web site: www.vectorlabs.com
bcl-2/VP-B201/rev 06-04