Potential hepatic stem cells reside in EpCAM cells of normal and
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
Immunological Memory: Contribution of Memory B Cells Expressing
... CD27⫹/CD80⫹ subsets. Highly purified (⬎97%) CD4⫹ T cells were isolated from fresh PBMCs by a two-step negative selection process using T cell subset enrichment columns (R&D Systems, Minneapolis, MN) followed by magnetic bead depletion of unwanted cells, as previously described (32). ...
... CD27⫹/CD80⫹ subsets. Highly purified (⬎97%) CD4⫹ T cells were isolated from fresh PBMCs by a two-step negative selection process using T cell subset enrichment columns (R&D Systems, Minneapolis, MN) followed by magnetic bead depletion of unwanted cells, as previously described (32). ...
Roles of FGFs as adipokines in adipose tissue development
... and metabolism (Wang et al., 2008; Galic et al., 2010). Although BAT also produces adipokines, the endocrine roles of BAT are needed to determine by further research (Villarroya et al., 2013). Fibroblast growth factors (FGFs) are signaling proteins with diverse functions in development, metabolism, ...
... and metabolism (Wang et al., 2008; Galic et al., 2010). Although BAT also produces adipokines, the endocrine roles of BAT are needed to determine by further research (Villarroya et al., 2013). Fibroblast growth factors (FGFs) are signaling proteins with diverse functions in development, metabolism, ...
Cellular origin of the basement membrane in embryonic chicken
... matrix was observed in absence of fibroblasts. a soluble collagenous protein could be recovered from the culture medium. These preliminary observations were confirmed by several authors. who showed that both myogenic and fibrogenic cells of mouse and quail skeletal muscles synthesize and secrete typ ...
... matrix was observed in absence of fibroblasts. a soluble collagenous protein could be recovered from the culture medium. These preliminary observations were confirmed by several authors. who showed that both myogenic and fibrogenic cells of mouse and quail skeletal muscles synthesize and secrete typ ...
- ATS Journals
... The G773D mutation was observed among seven additional family members of the proband’s extended pedigree (Figure 1d). Six of these seven additional carriers had evidence of airflow limitation (FEV1 39–79% predicted); all six were current or previous cigarette smokers. The one carrier with normal pul ...
... The G773D mutation was observed among seven additional family members of the proband’s extended pedigree (Figure 1d). Six of these seven additional carriers had evidence of airflow limitation (FEV1 39–79% predicted); all six were current or previous cigarette smokers. The one carrier with normal pul ...
Staining
... Tissue is cut in slides of one cell layer, it means 4-10mm. Tissue is translucent and „well- readable“ in this case Devices that are used for cutting are called microtomes. Tissue slices are put on slide. They are stretched out by heat, and stick by egg whiteglycerin ...
... Tissue is cut in slides of one cell layer, it means 4-10mm. Tissue is translucent and „well- readable“ in this case Devices that are used for cutting are called microtomes. Tissue slices are put on slide. They are stretched out by heat, and stick by egg whiteglycerin ...
1 - Purdue Department of Biological Sciences
... protoxylem, red bracket marks metaxylem. Bar=66 lm. c–j Cells numbered 1–4 show different developmental stages of xylem vessels, described in g. c In situ hybridization of ZeFLA11 sense riboprobe with an equivalent serial section as shown in b, showing nonspecific background labeling in stem bundle 12 ...
... protoxylem, red bracket marks metaxylem. Bar=66 lm. c–j Cells numbered 1–4 show different developmental stages of xylem vessels, described in g. c In situ hybridization of ZeFLA11 sense riboprobe with an equivalent serial section as shown in b, showing nonspecific background labeling in stem bundle 12 ...
Transformations
... genes for insulin and growth hormone into bacteria has created bacteria which obligingly produce as much human insulin and human growth hormone as we need. Scientists can also take DNA from a deadly organism, divide it into many pieces, and safely study the individual pieces by introducing the fragm ...
... genes for insulin and growth hormone into bacteria has created bacteria which obligingly produce as much human insulin and human growth hormone as we need. Scientists can also take DNA from a deadly organism, divide it into many pieces, and safely study the individual pieces by introducing the fragm ...
University of Groningen Effects of structure, morphology and
... The striped bars represent total fibrinogen measurements after 180 minutes of incubation with fibrinogen solution (n = 3). The amount of fibrinogen P2 epitope exposure on uncoated PET (BARE), poly(ethylene imine) coated PET (PEI), heparincoated PET (HEP) and poly(tetrafluoro ethylene) (PTFE) (n = 3) ...
... The striped bars represent total fibrinogen measurements after 180 minutes of incubation with fibrinogen solution (n = 3). The amount of fibrinogen P2 epitope exposure on uncoated PET (BARE), poly(ethylene imine) coated PET (PEI), heparincoated PET (HEP) and poly(tetrafluoro ethylene) (PTFE) (n = 3) ...
Chapter 7 Body Systems
... • Cytoplasm—thick gel-like substance inside of the cell composed of numerous organelles suspended in watery cytosol; each type of organelle (“little organ”) is suited to perform particular functions (Figure 3-2) ...
... • Cytoplasm—thick gel-like substance inside of the cell composed of numerous organelles suspended in watery cytosol; each type of organelle (“little organ”) is suited to perform particular functions (Figure 3-2) ...
Drosophila C-terminal Binding Protein Functions as a Context
... Groucho have been identified as Hairy-interacting proteins required for embryonic segmentation and Hairymediated transcriptional repression. While both dCtBP and Groucho are required for proper Hairy function, their properties are very different. As would be expected for a co-repressor, reduced Grou ...
... Groucho have been identified as Hairy-interacting proteins required for embryonic segmentation and Hairymediated transcriptional repression. While both dCtBP and Groucho are required for proper Hairy function, their properties are very different. As would be expected for a co-repressor, reduced Grou ...
Slide 1
... relatively stable internal environment • Humans as well as all animals need to maintain a homeostasis with their environment. • How does the human body maintain homeostasis? – The eleven organ systems of the human body work together to maintain homeostasis. – Homeostasis is the process by which orga ...
... relatively stable internal environment • Humans as well as all animals need to maintain a homeostasis with their environment. • How does the human body maintain homeostasis? – The eleven organ systems of the human body work together to maintain homeostasis. – Homeostasis is the process by which orga ...
Reduced Immunoregulatory CD31 T Cells in Patients With
... Exclusion criteria were: cancer, infection, and any other immunemediated disease. The protocol was approved by the local ethical committee (CCPPRB Paris-Cochin n° 2095, n° 1930 and n° 1931) and informed consent was obtained from all subjects before sampling. Of the 80 AAA patients included in the st ...
... Exclusion criteria were: cancer, infection, and any other immunemediated disease. The protocol was approved by the local ethical committee (CCPPRB Paris-Cochin n° 2095, n° 1930 and n° 1931) and informed consent was obtained from all subjects before sampling. Of the 80 AAA patients included in the st ...
Convergence and Extension Movements During Vertebrate
... within a plane of a single cell layer (planar) (Keller et al., 2000; Myers et al., 2002a), radial intercalations that occur between different cell layers can also contribute to C&E (Yin et al., 2008). In the process of radial intercalation, cells move between the cells in a deeper or more superficia ...
... within a plane of a single cell layer (planar) (Keller et al., 2000; Myers et al., 2002a), radial intercalations that occur between different cell layers can also contribute to C&E (Yin et al., 2008). In the process of radial intercalation, cells move between the cells in a deeper or more superficia ...
Tonic and burst firing: dual modes of
... responses to such an extent that this mode of firing prevents normal relay functions. According to this view, as an animal awakes (or is released from a pathological state), relay cells depolarize, thereby inactivating IT and promoting tonic firing, which is seen as the only useful relay mode. Howev ...
... responses to such an extent that this mode of firing prevents normal relay functions. According to this view, as an animal awakes (or is released from a pathological state), relay cells depolarize, thereby inactivating IT and promoting tonic firing, which is seen as the only useful relay mode. Howev ...
Characterization of chloroplasts in pavement cells of Arabidopsis
... The presence of PCC in Arabidopsis was best appreciated when the pavement cells were viewed from a lateral perspective. As shown in Fig. 1 this view was easily achieved for pavement cells lying at the edges of cotyledons and leaves. It allowed direct observation of the subcellular environment around ...
... The presence of PCC in Arabidopsis was best appreciated when the pavement cells were viewed from a lateral perspective. As shown in Fig. 1 this view was easily achieved for pavement cells lying at the edges of cotyledons and leaves. It allowed direct observation of the subcellular environment around ...
The Lymphatic System
... supporting cells • Lobules are lined with epithelial cells that secrete hormones called thymosins, which are involved in the differentiation of T cells • T cells that react to the body’s own cells undergo apoptosis (programmed cell death) • T cells that leave the thymus can react to foreign molecule ...
... supporting cells • Lobules are lined with epithelial cells that secrete hormones called thymosins, which are involved in the differentiation of T cells • T cells that react to the body’s own cells undergo apoptosis (programmed cell death) • T cells that leave the thymus can react to foreign molecule ...
Apoptosis-inducing factor is involved in the regulation of caspase
... cell death was associated with an approximate ninefold increase in caspase-3 activity at 16 h (Fig. 1 B), and this was accompanied by a significant increase in the fraction of cells exhibiting DNA fragmentation, as detected by TUNEL assay (50.2 vs. 12.3% in controls; Fig. 1 C). Consistent with a req ...
... cell death was associated with an approximate ninefold increase in caspase-3 activity at 16 h (Fig. 1 B), and this was accompanied by a significant increase in the fraction of cells exhibiting DNA fragmentation, as detected by TUNEL assay (50.2 vs. 12.3% in controls; Fig. 1 C). Consistent with a req ...
Stable benefit of embryonic stem cell therapy in - AJP
... signaling that supports cardiac-specific differentiation (3). Although understanding of the molecular mechanisms of stem cell commitment and integration with host myocardium is increasing, limited information is available on the natural history of stem cell therapy outcomes. We serially monitored th ...
... signaling that supports cardiac-specific differentiation (3). Although understanding of the molecular mechanisms of stem cell commitment and integration with host myocardium is increasing, limited information is available on the natural history of stem cell therapy outcomes. We serially monitored th ...
classification of connective tissue
... later stages of development of foetus the mesnechmye acquired abundant of fibers, the increased number of fibers in mesenchyme now called as Mucous, which widely distributed through out the body of embryo. ...
... later stages of development of foetus the mesnechmye acquired abundant of fibers, the increased number of fibers in mesenchyme now called as Mucous, which widely distributed through out the body of embryo. ...
AGI June 41/6 - American Journal of Physiology
... and 50 µg/ml of biotinylated ligand or biocytin. The particle mixtures were gently rocked overnight at 4°C and then transferred to a 0.5-ml Dispodialyzer dialysis tube (Spectrum, Houston, TX) with a molecular weight cut-off of 300,000. Excess unbound ligand was removed by dialysis against 4 l of PBS ...
... and 50 µg/ml of biotinylated ligand or biocytin. The particle mixtures were gently rocked overnight at 4°C and then transferred to a 0.5-ml Dispodialyzer dialysis tube (Spectrum, Houston, TX) with a molecular weight cut-off of 300,000. Excess unbound ligand was removed by dialysis against 4 l of PBS ...
MCAS Review Booklet
... These booklets are MCAS review booklets. Each strand from the Massachusetts State Frameworks is addressed with its corresponding chapter section (s) and pages from the textbook along with key concepts to answer and key vocabulary terms to define. In addition to the questions and vocabulary, you shou ...
... These booklets are MCAS review booklets. Each strand from the Massachusetts State Frameworks is addressed with its corresponding chapter section (s) and pages from the textbook along with key concepts to answer and key vocabulary terms to define. In addition to the questions and vocabulary, you shou ...
Developmental neurobiology of hydra, a model animal of cnidarians1
... system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the head and the basal end is the foot. The head has a hypostome with the mouth ope ...
... system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the head and the basal end is the foot. The head has a hypostome with the mouth ope ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.