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Intranasal Immunization With an Apolipoprotein B
... appears also to be targeted, but it remains unclear whether it is specific to LDL antigens. Finally, several LDL components are bioactive and proinflammatory and may, when injected, cause undesirable local or systemic effects. Therefore, immunization with immunodominant peptide sequences derived fro ...
... appears also to be targeted, but it remains unclear whether it is specific to LDL antigens. Finally, several LDL components are bioactive and proinflammatory and may, when injected, cause undesirable local or systemic effects. Therefore, immunization with immunodominant peptide sequences derived fro ...
Alamethicin permeabilizes the plasma membrane and mitochondria
... enzymes in their native proteinaceous environment with controlled cofactor concentrations. Possible uses and limitations of this method for plant cell research are discussed. ...
... enzymes in their native proteinaceous environment with controlled cofactor concentrations. Possible uses and limitations of this method for plant cell research are discussed. ...
MAUREEN CAROLINE McCANN Nationality
... machinery of the plant cell wall contributes to cell growth and specialization, and thus to the final stature and form of plants. The plant cell wall is a highly organized composite of many different polysaccharides, proteins, and aromatic substances that undergo dynamic changes during cell division ...
... machinery of the plant cell wall contributes to cell growth and specialization, and thus to the final stature and form of plants. The plant cell wall is a highly organized composite of many different polysaccharides, proteins, and aromatic substances that undergo dynamic changes during cell division ...
Ultra-High Resolution 3D Imaging of Whole Cells
... poor localization density because of the highly selective computational processes focusing on subtle features of PSFs. The methods are also susceptible to sample-induced optical aberrations, which change the PSF shape when imaging biological structures deeper in the sample (Burke et al., 2015; von D ...
... poor localization density because of the highly selective computational processes focusing on subtle features of PSFs. The methods are also susceptible to sample-induced optical aberrations, which change the PSF shape when imaging biological structures deeper in the sample (Burke et al., 2015; von D ...
Proteolytic Activation of Sterol Regulatory
... from the ER to the Golgi, thus blocking proteolytic cleavage and transcriptional activation of SREBPs. In cultured cells, the net result of Insig action is to decrease lipid synthesis whenever sterols accumulate to high levels within membranes. Human Insig-1 is composed of 277 amino acids (3), where ...
... from the ER to the Golgi, thus blocking proteolytic cleavage and transcriptional activation of SREBPs. In cultured cells, the net result of Insig action is to decrease lipid synthesis whenever sterols accumulate to high levels within membranes. Human Insig-1 is composed of 277 amino acids (3), where ...
Cnidaria Kat Hunter Piper
... Excretion- Cnidarians have a canalike cavity within their two layered bodies. For ingestion, digestion and egestion for food and waste. Reproduction- All cnidarians reproduce sexually. This occurs in one phase life cycle which is usually medusa. They can also reproduce asexually, but that occurs wit ...
... Excretion- Cnidarians have a canalike cavity within their two layered bodies. For ingestion, digestion and egestion for food and waste. Reproduction- All cnidarians reproduce sexually. This occurs in one phase life cycle which is usually medusa. They can also reproduce asexually, but that occurs wit ...
Using food and controlling growth - Delivery guide
... Learners often believe that plants photosynthesise in the day and only respire at night whilst others believe that leaves photosynthesise and roots respire. It could be valuable to start the topic by looking at respiration and why organisms undergo the process to make learners aware of the consequen ...
... Learners often believe that plants photosynthesise in the day and only respire at night whilst others believe that leaves photosynthesise and roots respire. It could be valuable to start the topic by looking at respiration and why organisms undergo the process to make learners aware of the consequen ...
Utilization of Amino Acids and Lack of Diazotrophy in
... aspartate, glutamate, glutamine, glycine and serine, when added to medium D, supported good growth of all strains (Table 1). Tyrosine and valine were utilized by all strains, but growth yields were lower on these substrates than on glutamate or aspartate. Certain other amino acids were used by one o ...
... aspartate, glutamate, glutamine, glycine and serine, when added to medium D, supported good growth of all strains (Table 1). Tyrosine and valine were utilized by all strains, but growth yields were lower on these substrates than on glutamate or aspartate. Certain other amino acids were used by one o ...
Efficient Endosomal Localization of Major Histocompatibility
... and 15-17 in the Ii cytosolic domain (Bakke and Dobberstein, 1990; Lotteau et al., 1990; Pieters et al., 1993; Odorizzi et al., 1994). When this signal is present, Ii complexed with class II exits the ER, is transported to and retained in a post-Golgi compartment and is degraded with a short half-li ...
... and 15-17 in the Ii cytosolic domain (Bakke and Dobberstein, 1990; Lotteau et al., 1990; Pieters et al., 1993; Odorizzi et al., 1994). When this signal is present, Ii complexed with class II exits the ER, is transported to and retained in a post-Golgi compartment and is degraded with a short half-li ...
Exocytosis and cell polarity in plants exocyst and recycling domains
... and plasma membrane proteins and those carrying periplasmic invertase. Although Bgl2 vesicles travel directly from TGN to the plasmalemma, invertase is transported through endosomal compartments (Harsay & Schekman, 2002). Which plant endomembrane system compartments produce exocytotic vesicles? The ...
... and plasma membrane proteins and those carrying periplasmic invertase. Although Bgl2 vesicles travel directly from TGN to the plasmalemma, invertase is transported through endosomal compartments (Harsay & Schekman, 2002). Which plant endomembrane system compartments produce exocytotic vesicles? The ...
Determination and morphogenesis in the sea urchin embryo
... is probably driven primarily by active cell repacking. The extracellular matrix is important for this repacking to occur, but the basis of the cellular-environmental interaction is not understood. There are new tools, especially well-defined specific antibodies and recombinant DNA clones, that may b ...
... is probably driven primarily by active cell repacking. The extracellular matrix is important for this repacking to occur, but the basis of the cellular-environmental interaction is not understood. There are new tools, especially well-defined specific antibodies and recombinant DNA clones, that may b ...
NEW Biology Part II CPR
... Meetings: 180 days Course Description The Biology course is designed to provide students with a detailed understanding of living systems. Emphasis continues to be placed on the skills necessary to examine alternative scientific explanations, actively conduct controlled experiments, analyze and commu ...
... Meetings: 180 days Course Description The Biology course is designed to provide students with a detailed understanding of living systems. Emphasis continues to be placed on the skills necessary to examine alternative scientific explanations, actively conduct controlled experiments, analyze and commu ...
Activation of DNA Damage Response Pathways during Lytic
... a core component of the non-homologous end-joining (NHEJ) pathway that acts throughout the cell cycle to repair DSBs [8]. DNA-PK is activated after the Ku70/Ku80 heterodimer binds DNA ends and recruits the DNA-PK catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme that is critical for complet ...
... a core component of the non-homologous end-joining (NHEJ) pathway that acts throughout the cell cycle to repair DSBs [8]. DNA-PK is activated after the Ku70/Ku80 heterodimer binds DNA ends and recruits the DNA-PK catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme that is critical for complet ...
Intelligent Monitoring System Satisfies Customer Needs for
... impedance/resistance or conductance as a diagnostic tool. The results of GNB’s study covering data collected using impedance meters from Elcorp and Alber and a conductance meter from Midtronics were reported recently [5]. It has been found that 1) impedance and conductance readings on new GNB Absoly ...
... impedance/resistance or conductance as a diagnostic tool. The results of GNB’s study covering data collected using impedance meters from Elcorp and Alber and a conductance meter from Midtronics were reported recently [5]. It has been found that 1) impedance and conductance readings on new GNB Absoly ...
IL-lra - Blood Journal
... monocytes and PMN, we examined the production of the protein. Cells were incubated with 20 ng/mL IL-13 for 18 hours and then secreted and cell-associated IL-Ira determined by a specific enzyme-linked immunosorbent assay (ELISA). As shown in Fig 5 , when cells were treated with IL-13, the production ...
... monocytes and PMN, we examined the production of the protein. Cells were incubated with 20 ng/mL IL-13 for 18 hours and then secreted and cell-associated IL-Ira determined by a specific enzyme-linked immunosorbent assay (ELISA). As shown in Fig 5 , when cells were treated with IL-13, the production ...
Identification of the Tuberous Sclerosis Complex
... phosphorylation of tuberin at sites recognized by this antibody slowly diminishes over the course of 10 hr. This is a similar time course to that seen for dephosphorylation of the well-characterized Akt substrates GSK3% and -$ following PDGF stimulation (Figure 2B, bottom). Tuberin is known to form ...
... phosphorylation of tuberin at sites recognized by this antibody slowly diminishes over the course of 10 hr. This is a similar time course to that seen for dephosphorylation of the well-characterized Akt substrates GSK3% and -$ following PDGF stimulation (Figure 2B, bottom). Tuberin is known to form ...
PPT - Altogen Biosystems
... Transfection Reagent (Glioblastoma Cells) Altogen Biosystems offers the U87 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The U87 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfectio ...
... Transfection Reagent (Glioblastoma Cells) Altogen Biosystems offers the U87 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The U87 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfectio ...
2-Cell and Molecular Biology (Plasma Membrane)
... Protein molecules that span the lipid bilayer mediate nearly all of other functions of membrane. For example: ...
... Protein molecules that span the lipid bilayer mediate nearly all of other functions of membrane. For example: ...
Biosynthesis and properties of the plant cell wall Wolf
... of a Ca2+-independent endo-1,4-β-glucanase restored cellulose synthesis, suggesting that this enzyme activity is required for cellulose formation in higher plants. Although SG appears to serve as a primer for cellulose synthesis in cotton fibers, it is not clear how far this observation can be gener ...
... of a Ca2+-independent endo-1,4-β-glucanase restored cellulose synthesis, suggesting that this enzyme activity is required for cellulose formation in higher plants. Although SG appears to serve as a primer for cellulose synthesis in cotton fibers, it is not clear how far this observation can be gener ...
The role of Mitogen Activated Protein Kinase (MAPK) - UiO
... form the major part of adipose tissue. Since obesity, which has reached epidemic proportions in the last decades, is the result of excess growth of the adipose tissue caused by both hypertrophy and hyperplasia, understanding how adipocytes expand is an important area research. Adipogenesis, the deve ...
... form the major part of adipose tissue. Since obesity, which has reached epidemic proportions in the last decades, is the result of excess growth of the adipose tissue caused by both hypertrophy and hyperplasia, understanding how adipocytes expand is an important area research. Adipogenesis, the deve ...
Negative regulation of the interferon response by
... ABSTRACT Long non-coding RNAs (lncRNAs) play critical roles in diverse cellular processes; however, their involvement in many critical aspects of the immune response including the interferon (IFN) response remains poorly understood. To address this gap, we compared the global gene expression pattern ...
... ABSTRACT Long non-coding RNAs (lncRNAs) play critical roles in diverse cellular processes; however, their involvement in many critical aspects of the immune response including the interferon (IFN) response remains poorly understood. To address this gap, we compared the global gene expression pattern ...
PDF
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
Protein Kinase C–dependent Activation of Cytosolic
... taining 1% N-P40, followed by two more washings in NP-40-free buffer. The pellets were finally resuspended either in SDS-loading buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, ...
... taining 1% N-P40, followed by two more washings in NP-40-free buffer. The pellets were finally resuspended either in SDS-loading buffer for SDS-PAGE and Western blotting or in the assay buffer supplemented with 5 mM DTT for the cPLA2 activity assay. Phosphorylation-induced mobility shift, SDS-PAGE, ...
Potential hepatic stem cells reside in EpCAM cells of normal and
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
... g/l collagenase type IV, 0.5 g/l pronase (Roche Diagnostics) and 50 mg/l DNase I (Sigma) by stirring for 20 minutes at 37°C. This digested liver was also passed through a 70-μm cell strainer and the flow-through fraction was combined with the first cell suspension. After centrifugation at 700 rpm (1 ...
Severe osmotic compression triggers a slowdown of
... association rates of several proteins inside the cell. Molecular crowding has received little attention in living cells, although it is usually invoked to explain why biochemical reactions rates may vary in vivo and in vitro (26–29). For instance, the diffusion coefficient of the green fluorescent pro ...
... association rates of several proteins inside the cell. Molecular crowding has received little attention in living cells, although it is usually invoked to explain why biochemical reactions rates may vary in vivo and in vitro (26–29). For instance, the diffusion coefficient of the green fluorescent pro ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.