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Full Text - The International Journal of Developmental Biology
... experimental analyses of chicken hematopoiesis, however, is still unsurpassed by any other model organism. The completion of chicken genome (International Chicken Genome Sequencing Consortium, 2004) has made molecular analysis relatively straightforward and genetic analysis possible. In addition to ...
... experimental analyses of chicken hematopoiesis, however, is still unsurpassed by any other model organism. The completion of chicken genome (International Chicken Genome Sequencing Consortium, 2004) has made molecular analysis relatively straightforward and genetic analysis possible. In addition to ...
March 28
... At Division of Informa@on Coding we run a project aimed at understanding (modelling) how a complex organism is developed from a single egg cell. We believe that this is fundamentally a ques@on about ...
... At Division of Informa@on Coding we run a project aimed at understanding (modelling) how a complex organism is developed from a single egg cell. We believe that this is fundamentally a ques@on about ...
Probes for Multiplexed Detection of GFP
... * This list covers only Aequoria victoria GFP, optimized mutants (e.g., EGFP) and green-fluorescent proteins from other species (e.g., Renilla reniformis). Fluorescent proteins with distinctly different excitation and emission characteristics (CFP, YFP, dsRed, etc.) are not included. Fluorescence ex ...
... * This list covers only Aequoria victoria GFP, optimized mutants (e.g., EGFP) and green-fluorescent proteins from other species (e.g., Renilla reniformis). Fluorescent proteins with distinctly different excitation and emission characteristics (CFP, YFP, dsRed, etc.) are not included. Fluorescence ex ...
PDF - The Journal of Immunology
... (60 mM) (Avanti Polar Lipids, Alabaster, AL) was added. After that, 570 ml of MeOH/CHCl3 (2:1, v/v) were added and samples were vortexed for 30 s followed by a 15-min sonication. Extraction was performed by sequential additions of H2O, CHCl3, and H2O (150, 190, and 160 ml, respectively). Then, sampl ...
... (60 mM) (Avanti Polar Lipids, Alabaster, AL) was added. After that, 570 ml of MeOH/CHCl3 (2:1, v/v) were added and samples were vortexed for 30 s followed by a 15-min sonication. Extraction was performed by sequential additions of H2O, CHCl3, and H2O (150, 190, and 160 ml, respectively). Then, sampl ...
Regulation of Ribosome Biogenesis by the Rapamycin
... Address after July 1, 1999: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616. ...
... Address after July 1, 1999: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616. ...
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats
... prevention of over-challenging the insulin secretory reserve of the endocrine pancreas such as in hyperthyroidism or during exogenous thyroid hormone administration [2]. This could be particularly important in people with a limited endogenous insulin secretory capacity who are most susceptible to th ...
... prevention of over-challenging the insulin secretory reserve of the endocrine pancreas such as in hyperthyroidism or during exogenous thyroid hormone administration [2]. This could be particularly important in people with a limited endogenous insulin secretory capacity who are most susceptible to th ...
Sperm entry is sufficient to trigger division of the
... plants were germinated in vitro and the number of sperm cells marked by HTR10-RFP was counted in each pollen tube (n300 for each data point, error bars indicate s.d.). (B)Expression of PromGEX1-GFP in wild-type pollen marks cytoplasm of the two sperm cells. (C)Expression of PromGEX1-GFP in cdka;1 ...
... plants were germinated in vitro and the number of sperm cells marked by HTR10-RFP was counted in each pollen tube (n300 for each data point, error bars indicate s.d.). (B)Expression of PromGEX1-GFP in wild-type pollen marks cytoplasm of the two sperm cells. (C)Expression of PromGEX1-GFP in cdka;1 ...
Lysosomal biogenesis and function is critical for necrotic cell death
... Bars represent the mean of three independent experiments. n > 300. Knockdown of both vacuolar H+-ATPase and aspartyl protease genes resulted in significantly more extended quenching of neurodegeneration than for any single gene. P < 0.001, unpaired t test. Efficacy of RNAi was assessed as described in ...
... Bars represent the mean of three independent experiments. n > 300. Knockdown of both vacuolar H+-ATPase and aspartyl protease genes resulted in significantly more extended quenching of neurodegeneration than for any single gene. P < 0.001, unpaired t test. Efficacy of RNAi was assessed as described in ...
Division, and Morphogenesis in Plants
... has accumulated a large body of data, and yet it remains a challenge to uncover the basic mechanisms that are operating. Early steps of development, when the zygote and its daughter cells organize the embryonic plant, are poorly understood despite considerable efforts toward the identification of re ...
... has accumulated a large body of data, and yet it remains a challenge to uncover the basic mechanisms that are operating. Early steps of development, when the zygote and its daughter cells organize the embryonic plant, are poorly understood despite considerable efforts toward the identification of re ...
View PDF - G3: Genes | Genomes | Genetics
... affects the synchrony of sporulation. Surprisingly, our approach for synchronous sporulation does not require growth in acetate-containing medium, but can be achieved in cells grown in rich medium until saturation. Our system requires solely IME1, because the expression of the N6-methyladenosine met ...
... affects the synchrony of sporulation. Surprisingly, our approach for synchronous sporulation does not require growth in acetate-containing medium, but can be achieved in cells grown in rich medium until saturation. Our system requires solely IME1, because the expression of the N6-methyladenosine met ...
Phylogenomics demonstrates that breviate flagellates are related to
... clan [7,18,19]. A breviate þ apusomonad (BA) clade was recovered in a 16-protein phylogenetic analysis, however the group was only weakly supported [20], a result that is perhaps unsurprising given that little data from apusomonads were included (only one to six genes were sampled). Analyses of a 15 ...
... clan [7,18,19]. A breviate þ apusomonad (BA) clade was recovered in a 16-protein phylogenetic analysis, however the group was only weakly supported [20], a result that is perhaps unsurprising given that little data from apusomonads were included (only one to six genes were sampled). Analyses of a 15 ...
lengthened g1 phase indicates differentiation status in
... Cell cycle, differentiation, and development Human embryonic stem cells (hESC) are defined by their ability to self-renew indefinitely in culture while maintaining the developmental potential to differentiate into any cell type representative of the adult body. A less commonly discussed defining cha ...
... Cell cycle, differentiation, and development Human embryonic stem cells (hESC) are defined by their ability to self-renew indefinitely in culture while maintaining the developmental potential to differentiate into any cell type representative of the adult body. A less commonly discussed defining cha ...
isolation, characterization, and expression of mouse icam
... a cell leukocyte integrins is demonstrated by a n inherited husurface glycoprotein, is a second counter-receptor man genetic deficiency, leukocyte adhesion deficiency, forlymphocyte function-associated Ag-1 (LFA-1). in whichlack of surface expressionof LFA- 1, Mac- 1, and We report here the isolatio ...
... a cell leukocyte integrins is demonstrated by a n inherited husurface glycoprotein, is a second counter-receptor man genetic deficiency, leukocyte adhesion deficiency, forlymphocyte function-associated Ag-1 (LFA-1). in whichlack of surface expressionof LFA- 1, Mac- 1, and We report here the isolatio ...
Quaternary ammonium surfactant structure determines selective
... Results: QAS toxicity normalized relative to surfactant critical micelle concentration showed n-dodecylpyridinium bromide (C12PB) to be the most effective, with a therapeutic index of ∼10 for an MDR strain of Escherichia coli and .20 for Neisseria gonorrhoeae after 1 h of exposure. Three modes of QA ...
... Results: QAS toxicity normalized relative to surfactant critical micelle concentration showed n-dodecylpyridinium bromide (C12PB) to be the most effective, with a therapeutic index of ∼10 for an MDR strain of Escherichia coli and .20 for Neisseria gonorrhoeae after 1 h of exposure. Three modes of QA ...
Profibrillin conversion by proprotein convertases
... times with 0.5% desoxycholate, washed once with distilled water and air dried. The extracellular matrix was scraped from the dish into 250 µl of reducing sample buffer. Media and the first desoxycholate lysate were precipitated with methanol/chloroform and pellets were redissolved in 250 µl of sampl ...
... times with 0.5% desoxycholate, washed once with distilled water and air dried. The extracellular matrix was scraped from the dish into 250 µl of reducing sample buffer. Media and the first desoxycholate lysate were precipitated with methanol/chloroform and pellets were redissolved in 250 µl of sampl ...
Diagnostic-Microbiology-4th-Edition
... c. Act as a virulence factor in helping the pathogen evade phagocytosis d. Provide an attachment site for somatic antigens. ANS: C The capsule acts as a virulence factor in helping the pathogen evade phagocytosis because antibodies have difficulty attaching to the capsule of bacteria and therefore a ...
... c. Act as a virulence factor in helping the pathogen evade phagocytosis d. Provide an attachment site for somatic antigens. ANS: C The capsule acts as a virulence factor in helping the pathogen evade phagocytosis because antibodies have difficulty attaching to the capsule of bacteria and therefore a ...
CRISPR/Cas9 as a tool for creation of p53 knock
... Mutations in the p53 gene are one of the most significant aspects of the origin of cancers. In order to reduce the potential dangers of mutated p53, artificial inhibition and stimulation of the cell’s normal DNA repair mechanisms have been considered. For example, Biddlestone-Thorpe et al. (2013) de ...
... Mutations in the p53 gene are one of the most significant aspects of the origin of cancers. In order to reduce the potential dangers of mutated p53, artificial inhibition and stimulation of the cell’s normal DNA repair mechanisms have been considered. For example, Biddlestone-Thorpe et al. (2013) de ...
FREE Sample Here - We can offer most test bank and
... 15. Why is the interior of the plasma membrane potentially impermeable to water-soluble molecules? a. The hydrophobic tails of the phospholipid molecules are found there. b. The hydrophilic tails of the phospholipid molecules are found there. c. The ion channels are found there. d. The cholesterol m ...
... 15. Why is the interior of the plasma membrane potentially impermeable to water-soluble molecules? a. The hydrophobic tails of the phospholipid molecules are found there. b. The hydrophilic tails of the phospholipid molecules are found there. c. The ion channels are found there. d. The cholesterol m ...
Cellular Receptors and Signal Transduction in Molluscan
... distinction of hemocyte subpopulations based on expression of a unique surface epitope also has been made in Lymnaea stagnalis; namely hemocytes expressing the monoclonal antibody LS1 surface marker are thought to represent undifferentiated cells (LS11 subset), whereas the LS12 cell subset is compri ...
... distinction of hemocyte subpopulations based on expression of a unique surface epitope also has been made in Lymnaea stagnalis; namely hemocytes expressing the monoclonal antibody LS1 surface marker are thought to represent undifferentiated cells (LS11 subset), whereas the LS12 cell subset is compri ...
Cellular and molecular mechanisms involved in branching
... these tip cells passively; during branch formation, all tracheal cells maintain their epithelial character and remain attached to each other via their subapical adherens junctions (AJ). It is not clear yet whether Bnl/FGF acts as a true chemoattractant or as a motogen. A function as a chemoattractan ...
... these tip cells passively; during branch formation, all tracheal cells maintain their epithelial character and remain attached to each other via their subapical adherens junctions (AJ). It is not clear yet whether Bnl/FGF acts as a true chemoattractant or as a motogen. A function as a chemoattractan ...
Achieving CNS axon regeneration by manipulating convergent
... Integrins are transmembrane heterodimeric receptors that facilitate bidirectional signaling between cells and the extracellular environment, most notably proteins found in the ECM or on other cells (e.g., laminin, fibronectin). Integrin binding activates intracellular signaling cascades that affect ...
... Integrins are transmembrane heterodimeric receptors that facilitate bidirectional signaling between cells and the extracellular environment, most notably proteins found in the ECM or on other cells (e.g., laminin, fibronectin). Integrin binding activates intracellular signaling cascades that affect ...
Fungi
... are, you do not think of ___________, ____________, ________ or ___________. However, all these things are made with the help of ___________. Other fungi can make us ________, such as the fungi that causes _____________ _________. Many fungi _____________ dead ____________ and dead ___________, and ...
... are, you do not think of ___________, ____________, ________ or ___________. However, all these things are made with the help of ___________. Other fungi can make us ________, such as the fungi that causes _____________ _________. Many fungi _____________ dead ____________ and dead ___________, and ...
Swimming behavior of the monotrichous bacterium Pseudomonas
... tumbles; Ping, 2012). ª 2013 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved ...
... tumbles; Ping, 2012). ª 2013 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved ...
Mitosis, Meiosis and Fertilization -
... separately. The top portion can then be reattached with Velcro, allowing it to be removed and swapped with the top portion of another sock. This can be particularly useful for teacher demonstrations. Potentially useful related activities (e.g. on the lifespan of different types of cells in the human ...
... separately. The top portion can then be reattached with Velcro, allowing it to be removed and swapped with the top portion of another sock. This can be particularly useful for teacher demonstrations. Potentially useful related activities (e.g. on the lifespan of different types of cells in the human ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.