Chapter 13 - Fission Yeast TOR and Rapamycin
... divide by medial fission, giving rise to two daughter cells of equal size. Upon either nitrogen, or carbon starvation, cells exit the logarithmic phase and can opt for two alternative pathways: entrance into quiescence (stationary phase) or sexual development. Either option results in cells (or spor ...
... divide by medial fission, giving rise to two daughter cells of equal size. Upon either nitrogen, or carbon starvation, cells exit the logarithmic phase and can opt for two alternative pathways: entrance into quiescence (stationary phase) or sexual development. Either option results in cells (or spor ...
RETINOBLASTOMA RELATED1 Regulates
... form all of the organs and cell types of wild-type plants (Figure 1A). However, when analyzing in detail the root architecture of these hypomorphic mutants, we found specific developmental defects. Whereas the formative division of a cortex-endodermis initial daughter is rapidly executed in the wild ...
... form all of the organs and cell types of wild-type plants (Figure 1A). However, when analyzing in detail the root architecture of these hypomorphic mutants, we found specific developmental defects. Whereas the formative division of a cortex-endodermis initial daughter is rapidly executed in the wild ...
growth of the plant cell wall - Personal.psu.edu
... Abstract | Plant cells encase themselves within a complex polysaccharide wall, which constitutes the raw material that is used to manufacture textiles, paper, lumber, films, thickeners and other products. The plant cell wall is also the primary source of cellulose, the most abundant and useful biopo ...
... Abstract | Plant cells encase themselves within a complex polysaccharide wall, which constitutes the raw material that is used to manufacture textiles, paper, lumber, films, thickeners and other products. The plant cell wall is also the primary source of cellulose, the most abundant and useful biopo ...
Functional coupling of FcRI to nicotinamide adenine
... profile of the RT-PCR products was not altered by treating cells with IFN-␥ or following differentiation (Figure 1A). At the protein level, Western blot analysis of immunoprecipitated PLDs revealed immunoreactive bands corresponding to the predicted molecular weights for PLD1a, PLD1b, and PLD2. The ...
... profile of the RT-PCR products was not altered by treating cells with IFN-␥ or following differentiation (Figure 1A). At the protein level, Western blot analysis of immunoprecipitated PLDs revealed immunoreactive bands corresponding to the predicted molecular weights for PLD1a, PLD1b, and PLD2. The ...
C. elegans MCM-4 is a general DNA replication and
... Reporters, transgenics and microscopy MCM-4::mCherry (Pmcm-4::MCM-4::mCherry::mcm-4 3'UTR) was generated by amplifying a 5.7 kb fragment encoding MCM-4 and 2.4 kb of promoter sequence from genomic N2 DNA using Phusion polymerase (Finnzymes) and cloning this fragment into the pGEMT vector (Promega). ...
... Reporters, transgenics and microscopy MCM-4::mCherry (Pmcm-4::MCM-4::mCherry::mcm-4 3'UTR) was generated by amplifying a 5.7 kb fragment encoding MCM-4 and 2.4 kb of promoter sequence from genomic N2 DNA using Phusion polymerase (Finnzymes) and cloning this fragment into the pGEMT vector (Promega). ...
Transport Systems in Living Things
... Figure 1B illustrates the important point that two or more substances diffuse independently of each other; that is, each diffuses down its own concentration gradient. Diffusion in Cells Diffusion is an important means by which materials are exchanged between a cell and its environment. For example, ...
... Figure 1B illustrates the important point that two or more substances diffuse independently of each other; that is, each diffuses down its own concentration gradient. Diffusion in Cells Diffusion is an important means by which materials are exchanged between a cell and its environment. For example, ...
Transient Recombinant Protein Expression in Mammalian Cells
... replicons have been further modified to chimera of two related viruses. Most details of the corresponding transfection systems have already been reviewed in detail previously (e.g. Smerdou and Liljeström 2000). Because of their specific properties, replicon-based gene expression is now mostly used ...
... replicons have been further modified to chimera of two related viruses. Most details of the corresponding transfection systems have already been reviewed in detail previously (e.g. Smerdou and Liljeström 2000). Because of their specific properties, replicon-based gene expression is now mostly used ...
Cancer-Initiating Cells from Colorectal Cancer Patients Escape from
... progression, and resistance to therapeutic agents (4–6); therefore, treatments aimed at blocking/destroying CICs should be pursued. Despite the advent of new diagnostic tools and targeted molecule agents, most CRC patients die of metastatic disease and almost half of patients receive chemotherapy wi ...
... progression, and resistance to therapeutic agents (4–6); therefore, treatments aimed at blocking/destroying CICs should be pursued. Despite the advent of new diagnostic tools and targeted molecule agents, most CRC patients die of metastatic disease and almost half of patients receive chemotherapy wi ...
Proposal for an enhanced, yeast
... surface display and fluorescence studies, respectively. Bacterial strains will be grown on LuriaBertani (LB) medium with appropriate antibiotics and yeast cells will grown and maintained on 1% yeast extract, 2% peptone, and 2% dextrose (YPD, Difco) broth or YPD agar at 30°C. Yeast recombinant trans ...
... surface display and fluorescence studies, respectively. Bacterial strains will be grown on LuriaBertani (LB) medium with appropriate antibiotics and yeast cells will grown and maintained on 1% yeast extract, 2% peptone, and 2% dextrose (YPD, Difco) broth or YPD agar at 30°C. Yeast recombinant trans ...
BIO 218 F 2012 CH 03 Martini Lecture Outline
... Epithelial Tissue Cells Squamous cells Thin, flat cells / “squished” nuclei Cuboidal cells Cube-shaped cells / centered, round nucleus Columnar cells Longer than they are wide / nucleus near the base Transitional cells Mixture of cells / nuclei appear to be scattered ...
... Epithelial Tissue Cells Squamous cells Thin, flat cells / “squished” nuclei Cuboidal cells Cube-shaped cells / centered, round nucleus Columnar cells Longer than they are wide / nucleus near the base Transitional cells Mixture of cells / nuclei appear to be scattered ...
ATCC is an independent, private, nonprofit biological
... in our broad range of cell lines, microorganisms, and viruses. Our system of maintaining seed stock and distribution stock - with quality control checks throughout the process - ensures that materials are as close as possible to the original culture with minimal transfers. All products are backed by ...
... in our broad range of cell lines, microorganisms, and viruses. Our system of maintaining seed stock and distribution stock - with quality control checks throughout the process - ensures that materials are as close as possible to the original culture with minimal transfers. All products are backed by ...
Role of N-Ras in Renal Fibrosis
... knockdown was assessed by qPCR. Toxicity was assessed by LDH assay. In vivo – CD1 male outbred mice were injected on Day1 and Day 21 with vehicle (V, normal saline) versus folic acid (FA, 125mg/kg, IV) to induce chronic folic acid induced nephropathy (FAIN) at Day 84. Serum samples were analyzed by ...
... knockdown was assessed by qPCR. Toxicity was assessed by LDH assay. In vivo – CD1 male outbred mice were injected on Day1 and Day 21 with vehicle (V, normal saline) versus folic acid (FA, 125mg/kg, IV) to induce chronic folic acid induced nephropathy (FAIN) at Day 84. Serum samples were analyzed by ...
Topical aldose reductase inhibitor for correcting
... routine eye examination was performed, and the subjects were excluded if any abnormal pathology that might affect the morphology of the corneal endothelium was noted. All of the 13 diabetic patients were found to have no diabetic retinopathy or simple (background) retinopathy at the pretreatment exa ...
... routine eye examination was performed, and the subjects were excluded if any abnormal pathology that might affect the morphology of the corneal endothelium was noted. All of the 13 diabetic patients were found to have no diabetic retinopathy or simple (background) retinopathy at the pretreatment exa ...
BIO 218 F 2012 CH 03 Martini Lecture Outline
... Epithelial Tissue Cells Squamous cells Thin, flat cells / “squished” nuclei Cuboidal cells Cube-shaped cells / centered, round nucleus Columnar cells Longer than they are wide / nucleus near the base Transitional cells Mixture of cells / nuclei appear to be scattered ...
... Epithelial Tissue Cells Squamous cells Thin, flat cells / “squished” nuclei Cuboidal cells Cube-shaped cells / centered, round nucleus Columnar cells Longer than they are wide / nucleus near the base Transitional cells Mixture of cells / nuclei appear to be scattered ...
Context-Dependent Synaptic Action of Glycinergic and GABAergic
... pipettes, the outer tip diameters of which ranged from 9 to 18 pm. The duration of pulses ranged from 3 to 800 msec at 15 psi. Glycine solutions were either 100 tnM, dissolved in distilled water, or 50 mM, substituted in equimolar concentrations for sodium chloride in physiological saline. Muscimol ...
... pipettes, the outer tip diameters of which ranged from 9 to 18 pm. The duration of pulses ranged from 3 to 800 msec at 15 psi. Glycine solutions were either 100 tnM, dissolved in distilled water, or 50 mM, substituted in equimolar concentrations for sodium chloride in physiological saline. Muscimol ...
Keshara Senanayake Ms.Reep AP BIOLOGY STUDY GUIDE
... are single celled eukaryotic organisms (like paramecia) large more complex organisms (plants/animals) are multicultural, their bodies are cooperatives of many kinds of specialized cells that could not survive for long on their own even when cells are arranged into higher levels of organization ( ...
... are single celled eukaryotic organisms (like paramecia) large more complex organisms (plants/animals) are multicultural, their bodies are cooperatives of many kinds of specialized cells that could not survive for long on their own even when cells are arranged into higher levels of organization ( ...
2017 Lecture PDF
... 1957 The invention of freeze fracture EM and the determination of membrane structure (http://jcb.rupress.org/cgi/content/full/168/2/174-a) Russell Steere introduces his home-made contraption for freeze fracture electron microscopy (EM), and Daniel Branton uses it to conclude that membranes are bilay ...
... 1957 The invention of freeze fracture EM and the determination of membrane structure (http://jcb.rupress.org/cgi/content/full/168/2/174-a) Russell Steere introduces his home-made contraption for freeze fracture electron microscopy (EM), and Daniel Branton uses it to conclude that membranes are bilay ...
Comparison of Autotransporter and Ice Nucleation Protein as Carrier
... many applications such as clinical diagnosis and therapeutics [1], which require antibodies with high antigen affinity and specificity [2]. To achieve this purpose, researchers invented many techniques to display engineered antibody fragments or full IgG on the surface of phage[3], yeast[4] or bacte ...
... many applications such as clinical diagnosis and therapeutics [1], which require antibodies with high antigen affinity and specificity [2]. To achieve this purpose, researchers invented many techniques to display engineered antibody fragments or full IgG on the surface of phage[3], yeast[4] or bacte ...
AtSpc98p and plant microtubule nucleation
... cells should be underlined. First, diverse MT arrays assemble successively, with different orientations during the cell cycle and/or developmental controls. The cortical MTs, the preprophase band and the phragmoplast are not found in other eukaryotic cells (Staiger and Lloyd, 1991; Lambert and Lloyd ...
... cells should be underlined. First, diverse MT arrays assemble successively, with different orientations during the cell cycle and/or developmental controls. The cortical MTs, the preprophase band and the phragmoplast are not found in other eukaryotic cells (Staiger and Lloyd, 1991; Lambert and Lloyd ...
Amino acid sequence identity between the HA1 of influenza A
... virus strains and their reassortant viruses containing the HA and neuraminidase of relevant epidemic strains (Maassab et al., 1985). Such viruses are under trial as live attenuated influenza vaccines (Wright et al., 1982; Murphy & Clements, 1989). Because potential reference and vaccine strains of v ...
... virus strains and their reassortant viruses containing the HA and neuraminidase of relevant epidemic strains (Maassab et al., 1985). Such viruses are under trial as live attenuated influenza vaccines (Wright et al., 1982; Murphy & Clements, 1989). Because potential reference and vaccine strains of v ...
Inhibition of Transdifferentiation into Tracheary Elements by Polar
... Similarly, we examined the restorative effects of 2,4-D and IAA. The addition of as high a concentration as 5–10 µM IAA (Fig. 3C), which is similar to concentrations of NAA necessary for the recovery of TE differentiation, overcame the suppression of TE formation by NPA. In contrast, as little as 0. ...
... Similarly, we examined the restorative effects of 2,4-D and IAA. The addition of as high a concentration as 5–10 µM IAA (Fig. 3C), which is similar to concentrations of NAA necessary for the recovery of TE differentiation, overcame the suppression of TE formation by NPA. In contrast, as little as 0. ...
Cell Wall Polysaccharides are Mislocalized to the Vacuole in
... SYP41–VTI12–SNARE [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor] complex to achieve proper targeting of post-Golgi vesicles (Stein et al. 2009, Gendre et al. 2011, Drakakaki et al. 2012). Recently, two YPT/RAB-interacting proteins (YIPs), YIP4a and YIP4b, have been ide ...
... SYP41–VTI12–SNARE [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor] complex to achieve proper targeting of post-Golgi vesicles (Stein et al. 2009, Gendre et al. 2011, Drakakaki et al. 2012). Recently, two YPT/RAB-interacting proteins (YIPs), YIP4a and YIP4b, have been ide ...
New type of snRNP containing nuclear bodies in plant cells
... microspore show a remarkable spatial separation of different macromolecules associated with the splicing system (snRNA and Sm proteins). This trait of the microspore nuclear bodies makes it possible to consider them as not only a convenient model for investigations on the assembly and disassembly of ...
... microspore show a remarkable spatial separation of different macromolecules associated with the splicing system (snRNA and Sm proteins). This trait of the microspore nuclear bodies makes it possible to consider them as not only a convenient model for investigations on the assembly and disassembly of ...
Agriculturae Conspectus Scientificus
... 3A and 3C). The callose deposits were quite abundant in cell walls of idioblasts (Figs 3B and 3D). No visible differences in callose deposits between idioblasts walls in spongy parenchyma of both cultivars were noticed, but there were significant differences (p < 0.05) in number of idioblasts per cr ...
... 3A and 3C). The callose deposits were quite abundant in cell walls of idioblasts (Figs 3B and 3D). No visible differences in callose deposits between idioblasts walls in spongy parenchyma of both cultivars were noticed, but there were significant differences (p < 0.05) in number of idioblasts per cr ...
The Importance of High Resolution Chromosome Analysis in the
... needs to be borne in mind when deciding the amount of the mitotic arrestant to be added. This is because the concentration of Colcemid® has been shown to be critical as it affects the mitotic index, chromosome spreading, and chromosome length.8,9 Another method of obtaining long chromosomes is to ad ...
... needs to be borne in mind when deciding the amount of the mitotic arrestant to be added. This is because the concentration of Colcemid® has been shown to be critical as it affects the mitotic index, chromosome spreading, and chromosome length.8,9 Another method of obtaining long chromosomes is to ad ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.