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New type of snRNP containing nuclear bodies in plant cells
... microspore show a remarkable spatial separation of different macromolecules associated with the splicing system (snRNA and Sm proteins). This trait of the microspore nuclear bodies makes it possible to consider them as not only a convenient model for investigations on the assembly and disassembly of ...
... microspore show a remarkable spatial separation of different macromolecules associated with the splicing system (snRNA and Sm proteins). This trait of the microspore nuclear bodies makes it possible to consider them as not only a convenient model for investigations on the assembly and disassembly of ...
Kingdom Protista A Mixed Bag of Organisms
... are made of _____________________(main ingredient of glass). ...
... are made of _____________________(main ingredient of glass). ...
Cell shape changes during gastrulation in
... event that folds a one-layered epithelium into a multilayered structure. In this paper, we describe the shape changes and behaviour of the cells participating in this process and show how mutations that change cell fate affect this behaviour. We divide the formation of the mesodermal germ layer into ...
... event that folds a one-layered epithelium into a multilayered structure. In this paper, we describe the shape changes and behaviour of the cells participating in this process and show how mutations that change cell fate affect this behaviour. We divide the formation of the mesodermal germ layer into ...
H3K4me3 demethylation by the histone
... H3K4me3. Fork activation and histone H4 acetylation, additional early events involved in DNA replication, are not affected by JARID1C downregulation. All together, these data point to a prominent role for JARID1C in a specific phase of DNA replication in mammalian cells, through its demethylase acti ...
... H3K4me3. Fork activation and histone H4 acetylation, additional early events involved in DNA replication, are not affected by JARID1C downregulation. All together, these data point to a prominent role for JARID1C in a specific phase of DNA replication in mammalian cells, through its demethylase acti ...
Sanchez pm
... under the embryonic amnioserosa, and cells of the subesophageal body (Sánchez et al., 1995). We have shown that Lazarillo has a unique role within the lipocalin family: it is involved in the guidance of developing axons. This role has been demonstrated for a particular pair of commissural neurons, t ...
... under the embryonic amnioserosa, and cells of the subesophageal body (Sánchez et al., 1995). We have shown that Lazarillo has a unique role within the lipocalin family: it is involved in the guidance of developing axons. This role has been demonstrated for a particular pair of commissural neurons, t ...
Programmed cell death in C. elegans: the genetic framework
... death (18). Strikingly, mutations in both ced-3 and ced-4 genes prevent most, if not all, programmed cell death in C. elegans, suggesting that both genes are required for the execution of cell death. Mosaic analysis of ced-3 and ced-4 mutants suggest that both genes act within dying cells to cause ...
... death (18). Strikingly, mutations in both ced-3 and ced-4 genes prevent most, if not all, programmed cell death in C. elegans, suggesting that both genes are required for the execution of cell death. Mosaic analysis of ced-3 and ced-4 mutants suggest that both genes act within dying cells to cause ...
Subcellular Localization and Activity of Multidrug
... could easily ascertain the phenotypes conferred by the protein of interest, because nonexpressing cells were present alongside cells expressing the protein and exposed to identical culture conditions. The ECFP tag on each protein permitted easy identification of those cells that expressed a drug tra ...
... could easily ascertain the phenotypes conferred by the protein of interest, because nonexpressing cells were present alongside cells expressing the protein and exposed to identical culture conditions. The ECFP tag on each protein permitted easy identification of those cells that expressed a drug tra ...
SUMO Pathway Modulation of Regulatory Protein Binding
... binding of Fob1 can have a large impact on multiple rDNAbinding proteins. The composition of rDNA chromatin changes through the cell cycle. During S phase, Fob1 functions as a unidirectional, physical block to the DNA replication machinery (Kobayashi and Horiuchi 1996). Paradoxically, Fob1 both is r ...
... binding of Fob1 can have a large impact on multiple rDNAbinding proteins. The composition of rDNA chromatin changes through the cell cycle. During S phase, Fob1 functions as a unidirectional, physical block to the DNA replication machinery (Kobayashi and Horiuchi 1996). Paradoxically, Fob1 both is r ...
6 December 2009
... 45 – 65 nm was formed by HEK293 cells grown on oxide surfaces coated by collagen (Straub et al. 2001) or 70 + 10 for HEK293 cells grown on fibronectincoated substrates (Brittinger & Fromherz 2005). A recent systematic study of the cleft width formed by HEK293 cells using thin sections prepared for tr ...
... 45 – 65 nm was formed by HEK293 cells grown on oxide surfaces coated by collagen (Straub et al. 2001) or 70 + 10 for HEK293 cells grown on fibronectincoated substrates (Brittinger & Fromherz 2005). A recent systematic study of the cleft width formed by HEK293 cells using thin sections prepared for tr ...
Sample pages 1 PDF
... have roles in cytokinesis, hormone responses, and pathogen resistance (Collins et al. 2003). Nine Qa-SNAREs are on the PM: SYP111/KNOLLE, SYP112, SYP121, SYP122, SYP123, SYP124, SYP125, SYP131, and SYP132 (Uemura et al. 2004). Some of these Qa-SNAREs show ubiquitous expression and uniform localizati ...
... have roles in cytokinesis, hormone responses, and pathogen resistance (Collins et al. 2003). Nine Qa-SNAREs are on the PM: SYP111/KNOLLE, SYP112, SYP121, SYP122, SYP123, SYP124, SYP125, SYP131, and SYP132 (Uemura et al. 2004). Some of these Qa-SNAREs show ubiquitous expression and uniform localizati ...
How plant cells explore geometry - The polarity induction and
... are investigated. A standardized system was developed to generate and integrate quantitative data on the temporal patterns of regeneration stages. Hereinafter this system was integrated into a microfluidic platform to study the impacts of chemical and geometrical stimuli during the establishment of ...
... are investigated. A standardized system was developed to generate and integrate quantitative data on the temporal patterns of regeneration stages. Hereinafter this system was integrated into a microfluidic platform to study the impacts of chemical and geometrical stimuli during the establishment of ...
Dynamic Localization of Rop GTPases to the
... interconnected and can be easily visualized under epifluorescence microscope (Fig. 1, C through F). Third, each tapetal cell contains several chamber-like structures that are stained with Rop antibodies, some chambers are bigger than the others (Fig. 1G). Certain chambers remain connected to neighbo ...
... interconnected and can be easily visualized under epifluorescence microscope (Fig. 1, C through F). Third, each tapetal cell contains several chamber-like structures that are stained with Rop antibodies, some chambers are bigger than the others (Fig. 1G). Certain chambers remain connected to neighbo ...
Biology (SPA)
... (1635–1703), one of the first scientists to use a microscope to examine pond water, cork and other things, was the first to refer to the cavities he saw in cork as ‘cells’, Latin for chambers. Subsequent scientists developed Hooke’s discovery of the cell into the Cell Theory on which modern Biology ...
... (1635–1703), one of the first scientists to use a microscope to examine pond water, cork and other things, was the first to refer to the cavities he saw in cork as ‘cells’, Latin for chambers. Subsequent scientists developed Hooke’s discovery of the cell into the Cell Theory on which modern Biology ...
Antimicrobial Agents and Chemotherapy
... (i) by counting the acid-precipitable activity or (ii) by chromatography of heat-inactivated samples as described previously (12, 15). The possibility that radioactive teichoic acid, in addition to peptidoglycan, was counted as acid-precipitable radioactivity or as a nonmoving component on the chrom ...
... (i) by counting the acid-precipitable activity or (ii) by chromatography of heat-inactivated samples as described previously (12, 15). The possibility that radioactive teichoic acid, in addition to peptidoglycan, was counted as acid-precipitable radioactivity or as a nonmoving component on the chrom ...
S-layer Structure in Bacteria and Archaea
... 2.2 SLP Composition and Post-Translational Modifications SLPs are distributed over a wide range of phylogenetic branches (Messner and Sleytr 1992; Sleytr et al. 1999) and generally show little (< 20 % identity) to no sequence similarity, even within a single species or closely related taxons. The ...
... 2.2 SLP Composition and Post-Translational Modifications SLPs are distributed over a wide range of phylogenetic branches (Messner and Sleytr 1992; Sleytr et al. 1999) and generally show little (< 20 % identity) to no sequence similarity, even within a single species or closely related taxons. The ...
Spermine Acts as a Negative Regulator of
... to bases ⫹7 to ⫺8 of the Nrf2 mRNA sequence. For the antisense experiments, cells were collected, washed in serum-free RPMI 1640 supplemented with 10 g/mL transferrin and 5 g/mL insulin and resuspended in the same medium at 2 ⫻ 104 cells/mL. Polyamines, 100 mol/L antisense oligonucleotides or a m ...
... to bases ⫹7 to ⫺8 of the Nrf2 mRNA sequence. For the antisense experiments, cells were collected, washed in serum-free RPMI 1640 supplemented with 10 g/mL transferrin and 5 g/mL insulin and resuspended in the same medium at 2 ⫻ 104 cells/mL. Polyamines, 100 mol/L antisense oligonucleotides or a m ...
MyoD as a gatekeeper to cell cycle progression
... Movement through the cell cycle is a highly coordinated process. A major mechanism of regulating this progression is the timely transcriptional activation of genes involved in each phase of the cell cycle. During development, MyoD directs cells fated for myoblast lineage to exit the cell cycle and b ...
... Movement through the cell cycle is a highly coordinated process. A major mechanism of regulating this progression is the timely transcriptional activation of genes involved in each phase of the cell cycle. During development, MyoD directs cells fated for myoblast lineage to exit the cell cycle and b ...
A protein kinase target of a PDK1 signalling pathway is involved in
... In animal cells, PDK1 is a central integrator for signalling events downstream of various receptors that stimulate the PI 3-kinase, and thus regulates processes, among which the most prevalent is the maintenance of balance between growth, cell division and apoptosis in animals (Alessi, 2001). So far ...
... In animal cells, PDK1 is a central integrator for signalling events downstream of various receptors that stimulate the PI 3-kinase, and thus regulates processes, among which the most prevalent is the maintenance of balance between growth, cell division and apoptosis in animals (Alessi, 2001). So far ...
pdf - John Innes Centre
... inessential structures (required only for growth under conditions of N-deprivation), it has been possible to identify mutations in “symbiotic genes” that are impaired in nodule development or function. Mutational analysis in genetically amenable model legumes such as Medicago truncatula, Lotus japon ...
... inessential structures (required only for growth under conditions of N-deprivation), it has been possible to identify mutations in “symbiotic genes” that are impaired in nodule development or function. Mutational analysis in genetically amenable model legumes such as Medicago truncatula, Lotus japon ...
Dendritic cells express tight junction proteins and penetrate gut
... open the tight junctions between epithelial cells, send dendrites outside the epithelium and directly sample bacteria. In addition, because DCs express tight-junction proteins such as occludin, claudin 1 and zonula occludens 1, the integrity of the epithelial barrier is preserved. that are ideally l ...
... open the tight junctions between epithelial cells, send dendrites outside the epithelium and directly sample bacteria. In addition, because DCs express tight-junction proteins such as occludin, claudin 1 and zonula occludens 1, the integrity of the epithelial barrier is preserved. that are ideally l ...
Author`s personal copy
... During development the speed of the cell cycle changes. Such cell cycle remodeling is important for many aspects of embryonic development including the morphogenetic cell movements of gastrulation, which can be prevented if cell division is not halted (Duncan and Su, 2004; Kurth, 2005; Murakami et a ...
... During development the speed of the cell cycle changes. Such cell cycle remodeling is important for many aspects of embryonic development including the morphogenetic cell movements of gastrulation, which can be prevented if cell division is not halted (Duncan and Su, 2004; Kurth, 2005; Murakami et a ...
Transplantation of porcine umbilical cord Matrix cells
... 60-day) at slaughter. Umbilical arteries and vein were stripped manually and discarded. The remaining tissue was minced finely in a sterile container in DMEM media with an antibiotic (Gentamycin, 20 g/ml, GIBCO BRL) and an antifungal agent (Amphotericin B 250 g/l, Sigma). The explants were transf ...
... 60-day) at slaughter. Umbilical arteries and vein were stripped manually and discarded. The remaining tissue was minced finely in a sterile container in DMEM media with an antibiotic (Gentamycin, 20 g/ml, GIBCO BRL) and an antifungal agent (Amphotericin B 250 g/l, Sigma). The explants were transf ...
Stem Cell - Active Motif
... The two hallmark features of stem cells are pluripotency, the ability to differentiate into any mature cell type, and self-renewal, the capacity to undergo indefinite replicative cycles without losing stem cell identity. The general types of stem cells include embryonic stem cells (ESCs), adult stem ...
... The two hallmark features of stem cells are pluripotency, the ability to differentiate into any mature cell type, and self-renewal, the capacity to undergo indefinite replicative cycles without losing stem cell identity. The general types of stem cells include embryonic stem cells (ESCs), adult stem ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.