Growing models of vertebrate limb development - ICB-USP
... embryo. One aspect of limb development that has perplexed several generations of researchers is the importance of growth. This might appear to be a trivial problem because growth occurs throughout the period when pattern is laid down and so, in the broadest sense, it is obviously required for develo ...
... embryo. One aspect of limb development that has perplexed several generations of researchers is the importance of growth. This might appear to be a trivial problem because growth occurs throughout the period when pattern is laid down and so, in the broadest sense, it is obviously required for develo ...
HIV-1 Replication by Distinct Mechanisms Monocyte
... were then washed, resuspended in DMEM containing pen/strep (1%), glutamine (1%), heat-inactivated FBS (10%), and heat-inactivated NHS (5%) (complete medium), and seeded into 75-cm2 flasks (Falcon; BD Biosciences Labware) at 8 ⫻ 106 cells/ml. Nonadherent cells, mostly T lymphocytes, were removed by g ...
... were then washed, resuspended in DMEM containing pen/strep (1%), glutamine (1%), heat-inactivated FBS (10%), and heat-inactivated NHS (5%) (complete medium), and seeded into 75-cm2 flasks (Falcon; BD Biosciences Labware) at 8 ⫻ 106 cells/ml. Nonadherent cells, mostly T lymphocytes, were removed by g ...
Evidence that FGF8 signalling from the midbrain
... A role for graded En expression in determining tectal polarity is further supported by grafting experiments in the chick. If the rostral mesencephalon is inverted, En-2 expression is reprogrammed in the grafted tissue to form a gradient consistent with its new environment (Martinez and AlvaradoMalla ...
... A role for graded En expression in determining tectal polarity is further supported by grafting experiments in the chick. If the rostral mesencephalon is inverted, En-2 expression is reprogrammed in the grafted tissue to form a gradient consistent with its new environment (Martinez and AlvaradoMalla ...
The Influence of Certain Trace Metals on Bacterial
... by monthly subculture on Evans peptone-agar slopes containing 0.2 yo glucose. Growth conditions. Cultures were grown in shaken flasks at 37" in either P medium (Webb, 1966) or T medium (Tempest et al. 1967). These solutions were supplemented with Mg2+and other ions as stated in the text and were ste ...
... by monthly subculture on Evans peptone-agar slopes containing 0.2 yo glucose. Growth conditions. Cultures were grown in shaken flasks at 37" in either P medium (Webb, 1966) or T medium (Tempest et al. 1967). These solutions were supplemented with Mg2+and other ions as stated in the text and were ste ...
View more Animal Life videos
... natural selection The process of differential survival and reproduction of Þtter genotypes; can be stabilizing, directional, or disruptive. Better adapted individuals are more likely to survive to reproductive age and thus leave more offspring and make a larger contribution to the gene pool than do ...
... natural selection The process of differential survival and reproduction of Þtter genotypes; can be stabilizing, directional, or disruptive. Better adapted individuals are more likely to survive to reproductive age and thus leave more offspring and make a larger contribution to the gene pool than do ...
Get PDF - Wiley Online Library
... and their excreted products attached to inert surfaces. In natural environments, these are mixed microbial cultures normally consisting predominantly of prokaryotes with some eukaryotes. Thus, as well as microbial cells, the surrounding milieu contains a range of macromolecular products in which exo ...
... and their excreted products attached to inert surfaces. In natural environments, these are mixed microbial cultures normally consisting predominantly of prokaryotes with some eukaryotes. Thus, as well as microbial cells, the surrounding milieu contains a range of macromolecular products in which exo ...
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... transformation of epithelium within the rudiment or from immigration from extrathymic sources. In an early experimental study of thymus organogenesis (Auerbach, 1960), embryonic thymus lobes from mice were dissociated into the epithelial primordium and the surrounding mesenchymal capsule, then cultu ...
... transformation of epithelium within the rudiment or from immigration from extrathymic sources. In an early experimental study of thymus organogenesis (Auerbach, 1960), embryonic thymus lobes from mice were dissociated into the epithelial primordium and the surrounding mesenchymal capsule, then cultu ...
Polarization of the endomembrane system is an early event in fucoid
... In most cell types examined, the actin cytoskeleton facilitates endocytosis [3,9]. The role of actin in polar endocytosis was examined by treatment with 30 nM Lat B, which depolymerizes actin filaments in S. compressa zygotes [8,15]. 6-h old zygotes were treated with Lat B for 1 h and then labeled w ...
... In most cell types examined, the actin cytoskeleton facilitates endocytosis [3,9]. The role of actin in polar endocytosis was examined by treatment with 30 nM Lat B, which depolymerizes actin filaments in S. compressa zygotes [8,15]. 6-h old zygotes were treated with Lat B for 1 h and then labeled w ...
Effect of ciliary neurotrophic factor (CNTF) on motoneuron survival
... in agreement with our own, i.e. no signal could be detected in Northern blots from chick tissues, including sciatic nerve (which contains high CNTF-like biological activity), using rat CNTF cDNA probes under low stringency hybridization conditions (C. Saldern, unpublished results). Therefore it is n ...
... in agreement with our own, i.e. no signal could be detected in Northern blots from chick tissues, including sciatic nerve (which contains high CNTF-like biological activity), using rat CNTF cDNA probes under low stringency hybridization conditions (C. Saldern, unpublished results). Therefore it is n ...
HELICOBACTER PYLORI VacA, A PARADIGM FOR TOXIN
... The importance of bacterial toxins in infectious diseases was recognized more than a century ago, when it was discovered that culture filtrates of many pathogens contain soluble factors that can damage host tissues1. Over the past century, more than one hundred different toxins that are produced by ...
... The importance of bacterial toxins in infectious diseases was recognized more than a century ago, when it was discovered that culture filtrates of many pathogens contain soluble factors that can damage host tissues1. Over the past century, more than one hundred different toxins that are produced by ...
Introduction Wnt signaling in development
... The development of C. elegans is invariant, meaning that each cell division always takes place at the same time in development and always generates the same daughter cells. The invariant nature of C. elegans development enabled John Sulston and others to fully describe the cell lineage that gives ri ...
... The development of C. elegans is invariant, meaning that each cell division always takes place at the same time in development and always generates the same daughter cells. The invariant nature of C. elegans development enabled John Sulston and others to fully describe the cell lineage that gives ri ...
Chapter 3: Cells
... (Outcome 3.19) F. Two types of proteins called ____________ also control cell division. (Outcome 3.17) G. When a cell becomes too large to obtain nutrients, it is likely to _______________________________________________________________________ . (Outcome 3.17) H. Two examples of external controls t ...
... (Outcome 3.19) F. Two types of proteins called ____________ also control cell division. (Outcome 3.17) G. When a cell becomes too large to obtain nutrients, it is likely to _______________________________________________________________________ . (Outcome 3.17) H. Two examples of external controls t ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... (passages 75–90) were grown in tissue culture flasks (75 cm2/250 ml) in 20 ml of DMEM supplemented with 10% (v/v) fetal calf serum with 1% penicillin/streptomycin. The osmolarity of the medium was 330 to 340 mOsM. Cells were grown at 37°C in a humidified atmosphere containing 5% CO2. The medium was ...
... (passages 75–90) were grown in tissue culture flasks (75 cm2/250 ml) in 20 ml of DMEM supplemented with 10% (v/v) fetal calf serum with 1% penicillin/streptomycin. The osmolarity of the medium was 330 to 340 mOsM. Cells were grown at 37°C in a humidified atmosphere containing 5% CO2. The medium was ...
Document
... elementary unit of life, organisms larger than unicellular protozoa arose as aggregates of such building units. Nature has experimented with producing larger organisms without cellular differentiation— certain large, single-celled marine algae, for example—but such examples are rarities. There are m ...
... elementary unit of life, organisms larger than unicellular protozoa arose as aggregates of such building units. Nature has experimented with producing larger organisms without cellular differentiation— certain large, single-celled marine algae, for example—but such examples are rarities. There are m ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... (passages 75–90) were grown in tissue culture flasks (75 cm2/250 ml) in 20 ml of DMEM supplemented with 10% (v/v) fetal calf serum with 1% penicillin/streptomycin. The osmolarity of the medium was 330 to 340 mOsM. Cells were grown at 37°C in a humidified atmosphere containing 5% CO2. The medium was ...
... (passages 75–90) were grown in tissue culture flasks (75 cm2/250 ml) in 20 ml of DMEM supplemented with 10% (v/v) fetal calf serum with 1% penicillin/streptomycin. The osmolarity of the medium was 330 to 340 mOsM. Cells were grown at 37°C in a humidified atmosphere containing 5% CO2. The medium was ...
The mitochondrial compartment - Plant Mitochondrial dynamics
... slot-like crista junctions have been measured at up to 200 nm, although the average length is 30–40 nm (Frey et al., 2002; Perkins et al., 1997a). The number of crista junctions and the morphology of the intercristal space have been shown to change with the metabolic state of the mitochondria (Hacke ...
... slot-like crista junctions have been measured at up to 200 nm, although the average length is 30–40 nm (Frey et al., 2002; Perkins et al., 1997a). The number of crista junctions and the morphology of the intercristal space have been shown to change with the metabolic state of the mitochondria (Hacke ...
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin 2
... Bloodgood, 2008). How the cilia regulate import and export of proteins is still unclear, but it might involve an electron-dense area at the base of the cilia, which is known as the transition zone. This area might function in a similar fashion to the tight junctions of epithelia, which specifically ...
... Bloodgood, 2008). How the cilia regulate import and export of proteins is still unclear, but it might involve an electron-dense area at the base of the cilia, which is known as the transition zone. This area might function in a similar fashion to the tight junctions of epithelia, which specifically ...
Research and Development
... the development of a detection system for assessing blackspot susceptibility. The detection system will form the basis of a kit for assessing the crop prior to harvest or during storage and will provide an 'early warning' of a high bruise risk. This will facilitate implementation of appropriate, gen ...
... the development of a detection system for assessing blackspot susceptibility. The detection system will form the basis of a kit for assessing the crop prior to harvest or during storage and will provide an 'early warning' of a high bruise risk. This will facilitate implementation of appropriate, gen ...
Paralytic Shellfish Poisoning Toxins: Biochemistry and Origin Masaaki Kodama
... ratio of the toxicity level to the cell number significantly varied, even in the same species. The ratio for the spring A. tamarense was generally larger than for that of summer, suggesting that both organsms differed substantially from each other in the amount of toxin per cell. It has been reporte ...
... ratio of the toxicity level to the cell number significantly varied, even in the same species. The ratio for the spring A. tamarense was generally larger than for that of summer, suggesting that both organsms differed substantially from each other in the amount of toxin per cell. It has been reporte ...
Organization of cardiac chamber progenitors in the zebrafish blastula
... positional tendencies correlate with a distribution of chamber specification signals within the primitive streak. In zebrafish, previous lineage analysis has shown that labeling of a single cardiogenic blastomere at the midblastula (2000 cell/high) stage results in labeled progeny in either the vent ...
... positional tendencies correlate with a distribution of chamber specification signals within the primitive streak. In zebrafish, previous lineage analysis has shown that labeling of a single cardiogenic blastomere at the midblastula (2000 cell/high) stage results in labeled progeny in either the vent ...
The GDP-bound form of Arf6 is located at the plasma membrane
... implicated in endocytosis and actin rearrangements at the cell periphery (Chavrier and Goud, 1999) but its precise function remains to be discovered. Two mutants of Arf6, Q67L and T27N, are considered to mimic the GTP- and GDP-bound forms, respectively, and have been used extensively to apprehend th ...
... implicated in endocytosis and actin rearrangements at the cell periphery (Chavrier and Goud, 1999) but its precise function remains to be discovered. Two mutants of Arf6, Q67L and T27N, are considered to mimic the GTP- and GDP-bound forms, respectively, and have been used extensively to apprehend th ...
Reperfusion of ischemic myocardium: Ultrastructural and
... common denominator observed in ischemic reperfused cardiac tissue was that functional recovery occurred only very slowly and often was absent (29-35). Many different pathophysiologic factors have been implicated In causing delayed functional recovery. Impaired mitochondrial function (29,31,36,37), p ...
... common denominator observed in ischemic reperfused cardiac tissue was that functional recovery occurred only very slowly and often was absent (29-35). Many different pathophysiologic factors have been implicated In causing delayed functional recovery. Impaired mitochondrial function (29,31,36,37), p ...
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... Annexins are also believed to be involved in membrane-related processes, such as intracellular vesicular trafficking, endo- and exocytosis, phagocytosis and autophagy, due to their inherent ability to bind and position the membrane structures in relation to each other in a calcium-dependent manner [ ...
... Annexins are also believed to be involved in membrane-related processes, such as intracellular vesicular trafficking, endo- and exocytosis, phagocytosis and autophagy, due to their inherent ability to bind and position the membrane structures in relation to each other in a calcium-dependent manner [ ...
Microbiology Focus 6.1 - The Role of - Sigma
... for susceptibility to this compound. Linezolid, another compound used against MRSA, may be of importance when treating serious infections by these bacteria, however, its activity against MRSA is only bacteriostatic. In these circumstances, the clinical potential of new drugs for treating MRSA infect ...
... for susceptibility to this compound. Linezolid, another compound used against MRSA, may be of importance when treating serious infections by these bacteria, however, its activity against MRSA is only bacteriostatic. In these circumstances, the clinical potential of new drugs for treating MRSA infect ...
Specific Organization of Golgi Apparatus in Plant Cells
... Abstract—Microtubules, actin filaments, and Golgi apparatus are connected both directly and indirectly, but it is manifested differently depending on the cell organization and specialization, and these connections are considered in many original studies and reviews. In this review we would like to d ...
... Abstract—Microtubules, actin filaments, and Golgi apparatus are connected both directly and indirectly, but it is manifested differently depending on the cell organization and specialization, and these connections are considered in many original studies and reviews. In this review we would like to d ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.