![An important role for glutathione and y](http://s1.studyres.com/store/data/015052761_1-0522192005ba491dbc6af379cdab36ed-300x300.png)
An important role for glutathione and y
... membrane of the yeast cell in the presence of cupric ion (Cu"). The cytoplasmic content of metabolic intermediates was estimated on the leakage fluid. The vacuolar content was then estimated by extraction of the Cu'+-permeabilized yeast with boiling water or trichloroacetic acid. The validity of the ...
... membrane of the yeast cell in the presence of cupric ion (Cu"). The cytoplasmic content of metabolic intermediates was estimated on the leakage fluid. The vacuolar content was then estimated by extraction of the Cu'+-permeabilized yeast with boiling water or trichloroacetic acid. The validity of the ...
Protozoa - Dr Magrann
... This infraciliature is not only found in Ciliophora, but also in all adult ciliates, even if adults lack external cilia. Structure of infraciliature is a primary tool in identifying different ciliate spp. ...
... This infraciliature is not only found in Ciliophora, but also in all adult ciliates, even if adults lack external cilia. Structure of infraciliature is a primary tool in identifying different ciliate spp. ...
ABSTRACT Title of Document:
... embryonic to posthatch transition. Glucose metabolism increased in both lymphocyte populations during the first two weeks posthatch due to increased glucose transporter-3 mRNA abundance, glucose uptake and hexokinase activity. Additionally, some of these metabolic markers were positively correlated ...
... embryonic to posthatch transition. Glucose metabolism increased in both lymphocyte populations during the first two weeks posthatch due to increased glucose transporter-3 mRNA abundance, glucose uptake and hexokinase activity. Additionally, some of these metabolic markers were positively correlated ...
Chromosome Segregation in Budding Yeast: Sister Chromatid
... Farcas et al. 2011). This provides further support for the topological embrace model and is consistent with the idea that sliding of cohesin along chromatin fibers is normally prevented by the presence of chromatin-bound proteins. The fact that 26-kb circular and 42-kb linear minichromosomes, which, ...
... Farcas et al. 2011). This provides further support for the topological embrace model and is consistent with the idea that sliding of cohesin along chromatin fibers is normally prevented by the presence of chromatin-bound proteins. The fact that 26-kb circular and 42-kb linear minichromosomes, which, ...
Lung fluid restriction affects growth but not airway branching of
... serumless medium. This suggests that the initial airway branch. ing process is controlled by intrinsic factors. Recent studies have implicated growth factors as intrinsic regulators of the branching process (Snead at al., 1989; Ganser at al.. 1991; Warburton at a/., 1992, 1993; Souza at a/., 1994). ...
... serumless medium. This suggests that the initial airway branch. ing process is controlled by intrinsic factors. Recent studies have implicated growth factors as intrinsic regulators of the branching process (Snead at al., 1989; Ganser at al.. 1991; Warburton at a/., 1992, 1993; Souza at a/., 1994). ...
Coexistence of organisms competing for the same substrate: An
... Chr. vinosum. The data suggest that Chr. weissei is able to sustain in mixed cultures by its high specific sulfide oxidation rate (q), whereas Chr. vinosurn does so by its high specific growth rate (/J). The two strains selected were thought to be representative. Data collected in the meanwhile do s ...
... Chr. vinosum. The data suggest that Chr. weissei is able to sustain in mixed cultures by its high specific sulfide oxidation rate (q), whereas Chr. vinosurn does so by its high specific growth rate (/J). The two strains selected were thought to be representative. Data collected in the meanwhile do s ...
Chemotactic Effect of Ciliary Neurotrophic Factor on Macrophages in
... microglia in neural damage and repair have been shown. Macrophages are often seen to associate with neurodegenerative diseases,16,17 but these cells can also be neuroprotective.18 The differential effects of macrophages under different conditions may be accounted for by the different phenotypes of t ...
... microglia in neural damage and repair have been shown. Macrophages are often seen to associate with neurodegenerative diseases,16,17 but these cells can also be neuroprotective.18 The differential effects of macrophages under different conditions may be accounted for by the different phenotypes of t ...
A Lectin with Highly Potent Inhibitory Activity toward Breast
... them exert immuno-modulatory activities [11,12], while others elicit anti-tumor, anti-bacterial, anti-fungal, anti-viral and antiinsect effects [13,14]. The physiological functions and mechanisms of various animal lectins have been studied precisely [15–18]. However, those of plant lectins have not ...
... them exert immuno-modulatory activities [11,12], while others elicit anti-tumor, anti-bacterial, anti-fungal, anti-viral and antiinsect effects [13,14]. The physiological functions and mechanisms of various animal lectins have been studied precisely [15–18]. However, those of plant lectins have not ...
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and
... Sec13:GFP, a COPII coat protein and ER export site marker, produced ER-associated fluorescent punctate structures that had an extremely short half-life of only ,10 s and seemed to form randomly over the ER. The number of such structures was also greater than the number of Golgi stacks, and the Golgi ...
... Sec13:GFP, a COPII coat protein and ER export site marker, produced ER-associated fluorescent punctate structures that had an extremely short half-life of only ,10 s and seemed to form randomly over the ER. The number of such structures was also greater than the number of Golgi stacks, and the Golgi ...
Formation and excretion of autophagic plastids (plastolysomes) in
... As seen in Figure 1B, these microspores entered an embryogenic program defined by several cell divisions that generated embryo-like structures. A qualitative (Figure 3) and quantitative (Table 1) study of plastids of these cells revealed that 60.7% of them presented conventional shapes, including ro ...
... As seen in Figure 1B, these microspores entered an embryogenic program defined by several cell divisions that generated embryo-like structures. A qualitative (Figure 3) and quantitative (Table 1) study of plastids of these cells revealed that 60.7% of them presented conventional shapes, including ro ...
Ceman, S, O Donnell, WT, Reed, M, Patton, S, Pohl, J and Warren, ST: Phosphorylation regulates translation state of FMRP-associated polyribosomes. Human Molecular Genetics 12:3295-3305 (2003).
... To determine whether mammalian FMRP is a phosphoprotein, we metabolically-labeled cells with [32P]orthophosphoric acid and isolated Flag-tagged FMRP by immunoprecipitation with an anti-Flag antibody as described previously (10). We observed an 80 kDa phosphoprotein in the Flag-FMRPexpressing transfe ...
... To determine whether mammalian FMRP is a phosphoprotein, we metabolically-labeled cells with [32P]orthophosphoric acid and isolated Flag-tagged FMRP by immunoprecipitation with an anti-Flag antibody as described previously (10). We observed an 80 kDa phosphoprotein in the Flag-FMRPexpressing transfe ...
Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome
... To further investigate the aberrant mobility of the putative treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 19 ...
... To further investigate the aberrant mobility of the putative treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 19 ...
Development of the lymphatic system: new questions and paradigms
... 2011; Yaniv et al., 2006). KD of prox1a using ATG antisense morpholino oligonucleotides (MOs) results in reduced numbers of PACs and a defective thoracic duct (Yaniv et al., 2006). By contrast, prox1a homozygous mutants display rather mild lymphatic defects (Koltowska et al., 2015a; van Impel et al. ...
... 2011; Yaniv et al., 2006). KD of prox1a using ATG antisense morpholino oligonucleotides (MOs) results in reduced numbers of PACs and a defective thoracic duct (Yaniv et al., 2006). By contrast, prox1a homozygous mutants display rather mild lymphatic defects (Koltowska et al., 2015a; van Impel et al. ...
... of the apatite minerals with a chemical formula of (Ca10(PO4)6(OH)2), is a major inorganic constituent about 6070 % of the inorganic portion of the bone matrix and possesses the high ability of ion-exchange against various cations which make that HAP has high biocompatibility and bioactivity propert ...
Exocytosis acts as a modulator of the ILT4
... were expressed on neutrophils. The myelomonoblast PLB-985 cell line is considered as an appropriate model to generate neutrophil-like cells upon differentiation with Dimethylformamide (DMF) or Dimethylsulfoxide (DMSO) treatment. As shown in Fig. 1C, most of undifferentiated PLB-985 cells did not exp ...
... were expressed on neutrophils. The myelomonoblast PLB-985 cell line is considered as an appropriate model to generate neutrophil-like cells upon differentiation with Dimethylformamide (DMF) or Dimethylsulfoxide (DMSO) treatment. As shown in Fig. 1C, most of undifferentiated PLB-985 cells did not exp ...
Cellulose Biosynthesis in Oomycetes
... Some species of Oomycetes are well studied pathogens that cause considerable economical losses in the agriculture and aquaculture industries. Currently, there are no chemicals available that are environmentally friendly and at the same time efficient Oomycete inhibitors. The ...
... Some species of Oomycetes are well studied pathogens that cause considerable economical losses in the agriculture and aquaculture industries. Currently, there are no chemicals available that are environmentally friendly and at the same time efficient Oomycete inhibitors. The ...
Department of Developmental and Cell Biology
... Genetics majors begin their study in the junior year with three required major courses (BIO SCI D103, BIO SCI D104) and (BIO SCI D113. In addition to these required major courses, students will choose six additional upper-division Biology Elective courses. Certain courses are designed to give studen ...
... Genetics majors begin their study in the junior year with three required major courses (BIO SCI D103, BIO SCI D104) and (BIO SCI D113. In addition to these required major courses, students will choose six additional upper-division Biology Elective courses. Certain courses are designed to give studen ...
Microbial Cell Factories
... also in almost all pathogenic infections, making them recalcitrant due to their multidrug resistance. Initially, the proposed function for cellulose in bacteria was not linked to biofilm formation. However, recent studies have revealed that some species of the family Enterobacteriaceae (e.g., Citrob ...
... also in almost all pathogenic infections, making them recalcitrant due to their multidrug resistance. Initially, the proposed function for cellulose in bacteria was not linked to biofilm formation. However, recent studies have revealed that some species of the family Enterobacteriaceae (e.g., Citrob ...
Further analysis on lidocaine-induced increase in intracellular Zn2+
... from Dojin Chemical Laboratory (Kumamoto, Japan). 2.2. Animals and cell preparation This study was approved by the Committee for Animal Experiments at the University of Tokushima (No. 05279). The cell suspension was prepared in a similar manner to that reported previously (Chikahisa et al., 1996; Ma ...
... from Dojin Chemical Laboratory (Kumamoto, Japan). 2.2. Animals and cell preparation This study was approved by the Committee for Animal Experiments at the University of Tokushima (No. 05279). The cell suspension was prepared in a similar manner to that reported previously (Chikahisa et al., 1996; Ma ...
Microtubules Contribute to Tubule Elongation and
... slower rate than the actin filament-based ER extension. Treatment with the actin-depolymerizing drug Latrunculin B made it possible to visualize the slow extension of the ER tubules in transgenic Arabidopsis (Arabidopsis thaliana) plants expressing ER-targeted green fluorescent protein. The ER tubules ...
... slower rate than the actin filament-based ER extension. Treatment with the actin-depolymerizing drug Latrunculin B made it possible to visualize the slow extension of the ER tubules in transgenic Arabidopsis (Arabidopsis thaliana) plants expressing ER-targeted green fluorescent protein. The ER tubules ...
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins
... neoformans, have been found to release virulence factors in vesicles, suggesting that this is a widely used strategy for pathogenic microbes to deliver a noxious cargo to target immune cells (12, 13). Many Gram-negative pathogenic bacterial species, including Pseudomonas aeruginosa, produce vesicles ...
... neoformans, have been found to release virulence factors in vesicles, suggesting that this is a widely used strategy for pathogenic microbes to deliver a noxious cargo to target immune cells (12, 13). Many Gram-negative pathogenic bacterial species, including Pseudomonas aeruginosa, produce vesicles ...
Induction of Apoptosis through B-cell Receptor Cross-linking
... caspases are activated through an apoptotic stimulus and subsequently activate downstream effector caspases. These effector caspases in turn have a multitude of intracellular substrates, among which are components that are critically needed for cellular homeostasis. Cleavage of one or more of these ...
... caspases are activated through an apoptotic stimulus and subsequently activate downstream effector caspases. These effector caspases in turn have a multitude of intracellular substrates, among which are components that are critically needed for cellular homeostasis. Cleavage of one or more of these ...
Graded potential of neural crest to form cornea, sensory neurons
... and Ayer-Le Lievre, 1982). This suggested that the cranial neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (M ...
... and Ayer-Le Lievre, 1982). This suggested that the cranial neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (M ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.