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Vibrio cholerae 01 Can Assume a Chlorine
... Rugose strains represent an interesting and still poorly understood morphologic variant of V. cholerae. Our data suggest ...
... Rugose strains represent an interesting and still poorly understood morphologic variant of V. cholerae. Our data suggest ...
chloroquine inhibits lysosomal enzyme pinocytosis and enhances
... provided direct evidence for Man-6-P in the recognition marker for human ß-glucuronidase, confirming the predictions based on the original indirect evidence . They also presented evidence that the Man-6-P was present on oligosaccharides released by endoglycosidase H . Related evidence has also been ...
... provided direct evidence for Man-6-P in the recognition marker for human ß-glucuronidase, confirming the predictions based on the original indirect evidence . They also presented evidence that the Man-6-P was present on oligosaccharides released by endoglycosidase H . Related evidence has also been ...
Applied and Environmental Microbiology
... medium and represented the large subpopulation of fully encapsulated single cells which uniformly bind the clover lectin trifoliin A. When 107 cells or more were added per seedling, bacteria attached within minutes, forming randomly oriented clumps at the root hair tips. Several hours later, single ...
... medium and represented the large subpopulation of fully encapsulated single cells which uniformly bind the clover lectin trifoliin A. When 107 cells or more were added per seedling, bacteria attached within minutes, forming randomly oriented clumps at the root hair tips. Several hours later, single ...
LIINA JAKOBSON The roles of abscisic acid, CO , and the cuticle in
... Land plants are crucial components of all terrestrial ecosystems on Earth. Thus, the colonization of land by plants was an essential event for the contemporary ecosystems. This was accompanied by a sequence of evolutionary adaptations necessary for plants to survive in a dry environment. The pathway ...
... Land plants are crucial components of all terrestrial ecosystems on Earth. Thus, the colonization of land by plants was an essential event for the contemporary ecosystems. This was accompanied by a sequence of evolutionary adaptations necessary for plants to survive in a dry environment. The pathway ...
Dual role of autophagy in HIV-1 replication and pathogenesis Open Access
... in the MOLT-4 CD4+ T cell line [45]. Infection of MOLT-4 cells with HIV-1NL4-3 (X4) or HIV-1NL4-Ad8, an R5 variant (i.e., using the CCR5 co-receptor), caused reductions in the number of autophagosomes visible by transmission electron microscopy (TEM) and in the levels of LC3-II as measured in Wester ...
... in the MOLT-4 CD4+ T cell line [45]. Infection of MOLT-4 cells with HIV-1NL4-3 (X4) or HIV-1NL4-Ad8, an R5 variant (i.e., using the CCR5 co-receptor), caused reductions in the number of autophagosomes visible by transmission electron microscopy (TEM) and in the levels of LC3-II as measured in Wester ...
Extrapolation of elementary rate constants of P
... fitting some weighted average. Importantly, P-gp mediated transport was not estimated by simply subtracting the transport in the presence of GF120918 from the total transport, which does not take back flow into consideration (Tran et al., 2005). Instead, passive permeability coefficients over time w ...
... fitting some weighted average. Importantly, P-gp mediated transport was not estimated by simply subtracting the transport in the presence of GF120918 from the total transport, which does not take back flow into consideration (Tran et al., 2005). Instead, passive permeability coefficients over time w ...
Sirtuins at a Glance - Journal of Cell Science
... (Nmnat) regenerates NAD from NMN (Houtkooper et al., 2010) (see poster). Recent studies suggest that NAD biosynthesis critically regulates the activity of sirtuins. For example, Nampt-mediated NAD biosynthesis controls SIRT1 activity in several tissues; and SIRT3, SIRT4 and SIRT5 are also regulated ...
... (Nmnat) regenerates NAD from NMN (Houtkooper et al., 2010) (see poster). Recent studies suggest that NAD biosynthesis critically regulates the activity of sirtuins. For example, Nampt-mediated NAD biosynthesis controls SIRT1 activity in several tissues; and SIRT3, SIRT4 and SIRT5 are also regulated ...
Targeted wild-type and jerker espins reveal a novel, WH2
... frequency and size were reduced. This was especially the case for the PC12 cells, which expressed each construct at a lower level than Neuro-2a cells. CABs were not observed in either cell line when the GFP-espin was expressed at the 2% level. Under these conditions, the GFP fluorescence was no long ...
... frequency and size were reduced. This was especially the case for the PC12 cells, which expressed each construct at a lower level than Neuro-2a cells. CABs were not observed in either cell line when the GFP-espin was expressed at the 2% level. Under these conditions, the GFP fluorescence was no long ...
Amino acid regulation of mTORC1 Liron Bar-Peled
... immunosuppressant (7), efficiently blocking IL-2 mediated lymphocyte proliferation at low nanogram doses (8), while maintaining immune surveillance (8) and thus avoiding additional complications associated with other immunosuppressants. Today, rapamycin (also known as serolimus) is a mainstay in org ...
... immunosuppressant (7), efficiently blocking IL-2 mediated lymphocyte proliferation at low nanogram doses (8), while maintaining immune surveillance (8) and thus avoiding additional complications associated with other immunosuppressants. Today, rapamycin (also known as serolimus) is a mainstay in org ...
Production and consumption of nitrous oxide in nitrate
... recombination of the wild-type (strain DSM 1740T) genome with a deletion plasmid (pDnosZ cat) designed to replace the entire nosZ gene in the nos operon by a chloramphenicol resistance gene cartridge (cat). For homologous recombination, the respective deletion plasmid contained cat flanked by two DN ...
... recombination of the wild-type (strain DSM 1740T) genome with a deletion plasmid (pDnosZ cat) designed to replace the entire nosZ gene in the nos operon by a chloramphenicol resistance gene cartridge (cat). For homologous recombination, the respective deletion plasmid contained cat flanked by two DN ...
The Drosophila ribbon gene encodes a nuclear BTB domain protein that promotes epithelial migration and morphogenesis. Development 128, 4923-4933. pdf
... primary branches. bnl encodes a FGF expressed by small clusters of cells surrounding each sac, at each position where a primary branch buds (Sutherland et al., 1996). Bnl serves as a chemoattractant, activating the Btl FGF receptor, a receptor tyrosine kinase expressed on the tracheal epithelium (Kl ...
... primary branches. bnl encodes a FGF expressed by small clusters of cells surrounding each sac, at each position where a primary branch buds (Sutherland et al., 1996). Bnl serves as a chemoattractant, activating the Btl FGF receptor, a receptor tyrosine kinase expressed on the tracheal epithelium (Kl ...
Spiralian Development: A Perspective Seventy
... difficulty arises from the fact that all representatives of the phyla involved do not show spiral cleavage. It is generally agreed that the forms showing clear-cut spiral cleavage by quartets include the polyclad turbellarians, the annelids, the rhynchocoels, the molluscs (with the notable exception ...
... difficulty arises from the fact that all representatives of the phyla involved do not show spiral cleavage. It is generally agreed that the forms showing clear-cut spiral cleavage by quartets include the polyclad turbellarians, the annelids, the rhynchocoels, the molluscs (with the notable exception ...
An Important Pool of Sucrose Linked to Starch Biosynthesis is Taken
... of glucose transferred to starch g–1 FW during the initial 90 min of culture, followed by an approximately 30 min asymptotic phase of low starch accumulation. Similar to the time course pattern of sucrose incorporation in sycamore cells (cf. Fig. 1, Etxeberria et al. 2005a), starch accumulation duri ...
... of glucose transferred to starch g–1 FW during the initial 90 min of culture, followed by an approximately 30 min asymptotic phase of low starch accumulation. Similar to the time course pattern of sucrose incorporation in sycamore cells (cf. Fig. 1, Etxeberria et al. 2005a), starch accumulation duri ...
Altered & Disordered Physiology - CH 056
... • Jaundice is not a disease state in itself • It is a non-specific symptom which is a feature of a range of disease states • The clinical approach to jaundice must be based on a clear understanding of the metabolism of bilirubin and an appreciation of the potential blocks which can occur in the path ...
... • Jaundice is not a disease state in itself • It is a non-specific symptom which is a feature of a range of disease states • The clinical approach to jaundice must be based on a clear understanding of the metabolism of bilirubin and an appreciation of the potential blocks which can occur in the path ...
A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a
... Death-associated protein 5 (DAP5) (also named p97 and NAT1) is a member of the translation initiation factor 4G (eIF4G) family that lacks the eIF4E binding site. It was previously implicated in apoptosis, based on the finding that a dominant negative fragment of the protein protected against cell de ...
... Death-associated protein 5 (DAP5) (also named p97 and NAT1) is a member of the translation initiation factor 4G (eIF4G) family that lacks the eIF4E binding site. It was previously implicated in apoptosis, based on the finding that a dominant negative fragment of the protein protected against cell de ...
An autophagy-related kinase is essential for the symbiotic
... To facilitate the acquisition of nutrients that are not readily available in the soil, most plants establish symbiotic interactions with soil microorganisms such as arbuscular mycorrhizal (AM) fungi of the monophyletic fungal lineage Glomeromycota and/or a wide range of gram-negative bacteria, colle ...
... To facilitate the acquisition of nutrients that are not readily available in the soil, most plants establish symbiotic interactions with soil microorganisms such as arbuscular mycorrhizal (AM) fungi of the monophyletic fungal lineage Glomeromycota and/or a wide range of gram-negative bacteria, colle ...
NORDINImmunomodulatoryEffects2012 - QMRO Home
... gene expression by HB02 or its fractions. ........................... 151 4.5.2 Quantitative RT-PCR reveals that HB02 and its fractions reduce iNOS mRNA level before and after LPS stimulation 153 4.5.3 HB02 and its fractions down-regulate iNOS protein levels more efficiently when the cells are treat ...
... gene expression by HB02 or its fractions. ........................... 151 4.5.2 Quantitative RT-PCR reveals that HB02 and its fractions reduce iNOS mRNA level before and after LPS stimulation 153 4.5.3 HB02 and its fractions down-regulate iNOS protein levels more efficiently when the cells are treat ...
Chato, a KRAB zinc-finger protein, regulates
... methods). chato mutant embryos arrested by E9.0 and remained unturned, with a short anterior-posterior body axis and an open gut tube (Fig. 1; see Fig. S1A,B in the supplementary material). Analysis of mesodermal tissues in chato embryos showed that defects in axis elongation were accompanied by a f ...
... methods). chato mutant embryos arrested by E9.0 and remained unturned, with a short anterior-posterior body axis and an open gut tube (Fig. 1; see Fig. S1A,B in the supplementary material). Analysis of mesodermal tissues in chato embryos showed that defects in axis elongation were accompanied by a f ...
Microtubule Independent Vesiculation of Golgi Membranes and the
... to Golgi is blocked at temperatures lower than 16°C, and the transport of proteins from the TGN en route to the cell surface is inhibited at temperatures below 20°C (Marlin and Simons, 1983; Saraste and Kuismanen, 1984; Tartakoff, 1986). We have found that while VGM production is inhibited at 160C, ...
... to Golgi is blocked at temperatures lower than 16°C, and the transport of proteins from the TGN en route to the cell surface is inhibited at temperatures below 20°C (Marlin and Simons, 1983; Saraste and Kuismanen, 1984; Tartakoff, 1986). We have found that while VGM production is inhibited at 160C, ...
Long-Distance Axonal Regeneration in the Transected Adult Rat
... 1991; Bovolenta et al., 1992; Giulian, 1993; Schwab et al., 1993). Although not all adult neurons exhibit the same regenerative potential, most of them regenerate their lesioned axons if the appropriate conditions are provided. Injured axons are able to grow for long distances through pieces of peri ...
... 1991; Bovolenta et al., 1992; Giulian, 1993; Schwab et al., 1993). Although not all adult neurons exhibit the same regenerative potential, most of them regenerate their lesioned axons if the appropriate conditions are provided. Injured axons are able to grow for long distances through pieces of peri ...
title of the thesis - UWSpace
... I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ...
... I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ...
Induction of light emission by luminescent bacteria treated with UV
... Introduction Light-emitting bacteria are the most abundant and widespread of luminescent organisms (MEIGHEN 1994). Most species of such bacteria live in marine environments (NEALSON 1978). It is well established that luminescent bacteria emit light effectively only when they are at high cell density ...
... Introduction Light-emitting bacteria are the most abundant and widespread of luminescent organisms (MEIGHEN 1994). Most species of such bacteria live in marine environments (NEALSON 1978). It is well established that luminescent bacteria emit light effectively only when they are at high cell density ...
Relationship of Net Chloride Flow across the Human Erythrocyte
... Blood was obtained from apparently healthy adults with heparin as an anticoagulant. The cells were washed three times in 160 mM NaCl and 5 mM HEPES at room temperature and the white cells were removed by aspiration . ...
... Blood was obtained from apparently healthy adults with heparin as an anticoagulant. The cells were washed three times in 160 mM NaCl and 5 mM HEPES at room temperature and the white cells were removed by aspiration . ...
Cytokinins regulate vascular morphogenesis in the Arabidopsis
... More than 400 million years ago, certain green algae began to adapt to living on land. These ancestors of the first terrestrial plants were able to cope with occasional drying, when growing on streams or mudflats, but they were still tightly depended on living on water. To successfully accomplish th ...
... More than 400 million years ago, certain green algae began to adapt to living on land. These ancestors of the first terrestrial plants were able to cope with occasional drying, when growing on streams or mudflats, but they were still tightly depended on living on water. To successfully accomplish th ...
ICAM-1 - The Journal of Cell Biology
... functions. Such functions include cell-ceU and cellmatrix interactions, cell motility, maintenance of cell polarity, and directed exocytosis and endocytosis. Most of the information regarding the transmembrane and cytoskeletal molecules that interact and support these functions originate from observ ...
... functions. Such functions include cell-ceU and cellmatrix interactions, cell motility, maintenance of cell polarity, and directed exocytosis and endocytosis. Most of the information regarding the transmembrane and cytoskeletal molecules that interact and support these functions originate from observ ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.