Stochastic and reversible aggregation of mRNA with expanded CUG
... Previous studies have shown that one can measure the dynamics of the MS2-tagged RNA itself, and not the exchange rate of the MS2–GFP molecule on the MS2 stem-loop, by using FRAP (Boireau et al., 2007; Mollet et al., 2008; Shav-Tal et al., 2004). With this system, we were thus able to perform FRAP an ...
... Previous studies have shown that one can measure the dynamics of the MS2-tagged RNA itself, and not the exchange rate of the MS2–GFP molecule on the MS2 stem-loop, by using FRAP (Boireau et al., 2007; Mollet et al., 2008; Shav-Tal et al., 2004). With this system, we were thus able to perform FRAP an ...
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... Environmental Science, Hangzhou Normal University, Hangzhou 311121, China. *These authors contributed equally to this work ...
... Environmental Science, Hangzhou Normal University, Hangzhou 311121, China. *These authors contributed equally to this work ...
The p57 CDKi integrates stress signals into cellcycle progression to
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
Assessments
... a statement that describes what always happens under certain conditions in nature. an explanation for events that are generally accepted as true. ...
... a statement that describes what always happens under certain conditions in nature. an explanation for events that are generally accepted as true. ...
golgi apparatus, gerl, and lysosomes of neurons in rat dorsal root
... Chemical Co.) ; 7 ml distilled water ; 10 ml 0 .2 M Tris-maleate buffer, pH 7 .2 ; 5 ml 0 .025 M manganese chloride ; and 3 ml 1 % lead nitrate . The medium is filtered after 5-10 min, and renewed after 30 min, when longer incubation times are used . Extensive observations were made of TPPaseincubat ...
... Chemical Co.) ; 7 ml distilled water ; 10 ml 0 .2 M Tris-maleate buffer, pH 7 .2 ; 5 ml 0 .025 M manganese chloride ; and 3 ml 1 % lead nitrate . The medium is filtered after 5-10 min, and renewed after 30 min, when longer incubation times are used . Extensive observations were made of TPPaseincubat ...
Assembling Neural Crest Regulatory Circuits into a Gene
... organisms ranging from lampreys and fish to frog, chick, and mouse. Neural crest cells form over a lengthy period of time during development that starts at gastrulation and extends into late organogenesis. This process is initiated by a combination of inductive signals emanating from environing tissu ...
... organisms ranging from lampreys and fish to frog, chick, and mouse. Neural crest cells form over a lengthy period of time during development that starts at gastrulation and extends into late organogenesis. This process is initiated by a combination of inductive signals emanating from environing tissu ...
Endoplasmic Microtubules Configure the Subapical Cytoplasm and
... hair, the density of CMTs is higher in growing root hairs than in hairs of other developmental stages. EMTs are located exclusively in the subapical cytoplasmic dense region between the basal part of the nucleus and the tip of the hair in a densely structured three-dimensional array (Fig. 3, D and F ...
... hair, the density of CMTs is higher in growing root hairs than in hairs of other developmental stages. EMTs are located exclusively in the subapical cytoplasmic dense region between the basal part of the nucleus and the tip of the hair in a densely structured three-dimensional array (Fig. 3, D and F ...
A Simple and Rapid Protocol for Producing Yeast Extract from
... Zarei O et al. / IJPR (2016), 15 (4): 907-913 ...
... Zarei O et al. / IJPR (2016), 15 (4): 907-913 ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Cdc42 in embryos using the conditional Gal4-UAS system. An Engrailed-Gal4 driver was used to express the Rho proteins in stripes, along with green fluorescent protein to visualize the overexpression domains. Stripes of ectopic protein accumulation could be seen above the uniform endogenous Rho1 leve ...
... Cdc42 in embryos using the conditional Gal4-UAS system. An Engrailed-Gal4 driver was used to express the Rho proteins in stripes, along with green fluorescent protein to visualize the overexpression domains. Stripes of ectopic protein accumulation could be seen above the uniform endogenous Rho1 leve ...
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... distinct parts: the interstitial fluid that directly surrounds the cell, and the plasma, the fluid portion of the blood. Materials are exchanged between the plasma and interstitial fluid, and between the interstitial fluid and the intracellular fluid (ICF). PTS: 1 3. Compare negative and positive fe ...
... distinct parts: the interstitial fluid that directly surrounds the cell, and the plasma, the fluid portion of the blood. Materials are exchanged between the plasma and interstitial fluid, and between the interstitial fluid and the intracellular fluid (ICF). PTS: 1 3. Compare negative and positive fe ...
Lignin biosynthesis in Norway spruce: from a model system to the tree
... Conservation of the putative substrate-binding amino acids was observed when the spruce peroxidase sequences were compared with other peroxidases with high affinity to coniferyl alcohol. We also used different peroxidase fractions to produce synthetic in vitro lignins from coniferyl alcohol; however ...
... Conservation of the putative substrate-binding amino acids was observed when the spruce peroxidase sequences were compared with other peroxidases with high affinity to coniferyl alcohol. We also used different peroxidase fractions to produce synthetic in vitro lignins from coniferyl alcohol; however ...
Two lineage boundaries coordinate vertebrate apical ectodermal
... CreERT transgene (Fig. 3J), eight transmitted the transgene, and four expressed CreERT in the AER, as determined by RNA in situ hybridization. The expression initiated at 9.5 dpc in the forelimbs and 10.0 dpc in the hindlimbs (stage 0.5/1) broadly in the ventral ectoderm and with development express ...
... CreERT transgene (Fig. 3J), eight transmitted the transgene, and four expressed CreERT in the AER, as determined by RNA in situ hybridization. The expression initiated at 9.5 dpc in the forelimbs and 10.0 dpc in the hindlimbs (stage 0.5/1) broadly in the ventral ectoderm and with development express ...
Ectopic Expression of AINTEGUMENTA in Arabidopsis Plants
... Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transformants, most were wild-type in appearance (58), while three had phenotypes resembling ant mutants. The plants with ant phenotypes may arise from cosuppression [re ...
... Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transformants, most were wild-type in appearance (58), while three had phenotypes resembling ant mutants. The plants with ant phenotypes may arise from cosuppression [re ...
Mathematical Biology
... filament barbed ends are associated with the membrane of the cell (Small et al., 1995a). However, whether the filaments are of uniform length, or whether there is a distribution of pointed ends and barbed ends throughout the lamellipod is an outstanding question (Heath and Holifield, 1991). The diff ...
... filament barbed ends are associated with the membrane of the cell (Small et al., 1995a). However, whether the filaments are of uniform length, or whether there is a distribution of pointed ends and barbed ends throughout the lamellipod is an outstanding question (Heath and Holifield, 1991). The diff ...
Strategies of aerobic ammonia-oxidizing bacteria
... As determined by 16S rRNA gene sequencing analysis, the phylogeny of autotrophic ammonia oxidizers comprises two monophyletic assemblages, one within the Gamma- and one within the Betaproteobacteria (Head et al., 1993; Teske et al., 1994; Purkhold et al., 2000). AOB within the Betaproteobacteria com ...
... As determined by 16S rRNA gene sequencing analysis, the phylogeny of autotrophic ammonia oxidizers comprises two monophyletic assemblages, one within the Gamma- and one within the Betaproteobacteria (Head et al., 1993; Teske et al., 1994; Purkhold et al., 2000). AOB within the Betaproteobacteria com ...
1 review 1 article
... by changing the diurnal L / D phases (Huang e t al., 1991) or by exposing the culture to a pulse of low temperature (Chen e t al., 1991 ;Grobbelaar & Huang, 1992). The freerunning periods of the nitrogenase activity and leucineuptake rhythms were found to be temperature compensated when the temperat ...
... by changing the diurnal L / D phases (Huang e t al., 1991) or by exposing the culture to a pulse of low temperature (Chen e t al., 1991 ;Grobbelaar & Huang, 1992). The freerunning periods of the nitrogenase activity and leucineuptake rhythms were found to be temperature compensated when the temperat ...
Characterization of the Visceral Endoderm Components in Early
... Curran, Joy Riley, Kristina Gonzalez and Sakthi Balaji for their friendship. I would like to show my gratitude to the members of my TRAC, Greg Pazour, Brian Lewis, Hong Zhang and James Li for their guidance and valuable suggestions over the years and also to Isabel Dominguez for contributing her tim ...
... Curran, Joy Riley, Kristina Gonzalez and Sakthi Balaji for their friendship. I would like to show my gratitude to the members of my TRAC, Greg Pazour, Brian Lewis, Hong Zhang and James Li for their guidance and valuable suggestions over the years and also to Isabel Dominguez for contributing her tim ...
NIH Public Access - Khademhosseini Lab
... addition of functionalized nanoparticles, in most studies nanoparticles were used as physical fillers in the polymer networks, and were not covalently incorporated into the resulting materials. The covalent crosslinking between polymer and nanoparticles enables the transfer of mechanical force withi ...
... addition of functionalized nanoparticles, in most studies nanoparticles were used as physical fillers in the polymer networks, and were not covalently incorporated into the resulting materials. The covalent crosslinking between polymer and nanoparticles enables the transfer of mechanical force withi ...
The SOX9 upstream region prone to chromosomal aberrations
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re- ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re- ...
The Role Of The Planar Cell Polarity Pathway In The Second Heart
... The heart of creatures is the foundation of life, the Prince of all, the sun of their microcosm, from where all vigor and strength does flow. —William Harvey, De Motu Cordis, 1628 ...
... The heart of creatures is the foundation of life, the Prince of all, the sun of their microcosm, from where all vigor and strength does flow. —William Harvey, De Motu Cordis, 1628 ...
science - Christian Schools International
... For us as Christians, all science should be conducted within a larger framework, or worldview, based on faith. The Bible is the divinely inspired record of God’s redemptive work on behalf of his people; it contains all that is necessary for bringing us to salvation and instructing us about how to l ...
... For us as Christians, all science should be conducted within a larger framework, or worldview, based on faith. The Bible is the divinely inspired record of God’s redemptive work on behalf of his people; it contains all that is necessary for bringing us to salvation and instructing us about how to l ...
The Populus homeobox gene ARBORKNOX2 regulates
... Compared with the cambium, the regulation of the shoot apical meristem (SAM) is increasingly well understood (Williams and Fletcher, 2005; Scheres, 2007). Class-I KNOX transcription factors are well characterized for their roles in regulating the SAM. The Arabidopsis class-I KNOX homeobox gene SHOOT ...
... Compared with the cambium, the regulation of the shoot apical meristem (SAM) is increasingly well understood (Williams and Fletcher, 2005; Scheres, 2007). Class-I KNOX transcription factors are well characterized for their roles in regulating the SAM. The Arabidopsis class-I KNOX homeobox gene SHOOT ...
FOCAL ADHESION KINASE: IN COMMAND AND CONTROL OF
... be connected to alterations in the polymerization or stabilization of actin and microtubule filaments. Additionally, because migrating cells experience changes in forces through integrin contacts that link the ECM with the cytoskeleton, FAK is important in the ‘sensing’ of mechanical forces that are ...
... be connected to alterations in the polymerization or stabilization of actin and microtubule filaments. Additionally, because migrating cells experience changes in forces through integrin contacts that link the ECM with the cytoskeleton, FAK is important in the ‘sensing’ of mechanical forces that are ...
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... death are unclear in most cases, they may involve the targeted destruction of factors required for cell survival (Yu et al., 2006). Future studies are required to understand the different contexts under which autophagy can promote either cell survival or destruction. Cell growth ...
... death are unclear in most cases, they may involve the targeted destruction of factors required for cell survival (Yu et al., 2006). Future studies are required to understand the different contexts under which autophagy can promote either cell survival or destruction. Cell growth ...
Auxin Transport Promotes Arabidopsis Lateral Root Initiation
... within adjacent files also contribute to the formation of the lateral root primordium (Figure 1B). The first detectable division to occur within a single file of xylem pole pericycle cells was transverse and asymmetric. Almost simultaneously, another asymmetric transverse division occurred in an adj ...
... within adjacent files also contribute to the formation of the lateral root primordium (Figure 1B). The first detectable division to occur within a single file of xylem pole pericycle cells was transverse and asymmetric. Almost simultaneously, another asymmetric transverse division occurred in an adj ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.