Interactive Textbook - St. Helens School District
... All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Requests for permission to make ...
... All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Requests for permission to make ...
Auxin coordinates cell division and cell fate specification during
... facilitating lateral root emergence. Furthermore NAC1 is targeted for degradation after ubiquitination through interaction with the E3-ligase SINAT5 (Xie et al., 2002). More recently, it was shown that the microRNA, miR164, targets several NAC-domain transcription factors, among which NAC1 (Mallory ...
... facilitating lateral root emergence. Furthermore NAC1 is targeted for degradation after ubiquitination through interaction with the E3-ligase SINAT5 (Xie et al., 2002). More recently, it was shown that the microRNA, miR164, targets several NAC-domain transcription factors, among which NAC1 (Mallory ...
Downloaded - The Journal of Cell Biology
... minal and 91 aa COOH-terminal regions. We compared fly p120 to the seven mammalian p120 family members and the single p120 homologues in C. elegans (jac-1) and the mosquito (A. gambiae) using ClustalW and Treeview to create an unrooted tree (Fig. 1 C). Several other Arm repeat proteins were included ...
... minal and 91 aa COOH-terminal regions. We compared fly p120 to the seven mammalian p120 family members and the single p120 homologues in C. elegans (jac-1) and the mosquito (A. gambiae) using ClustalW and Treeview to create an unrooted tree (Fig. 1 C). Several other Arm repeat proteins were included ...
Auxin coordinates cell division and cell fate specification during
... facilitating lateral root emergence. Furthermore NAC1 is targeted for degradation after ubiquitination through interaction with the E3-ligase SINAT5 (Xie et al., 2002). More recently, it was shown that the microRNA, miR164, targets several NAC-domain transcription factors, among which NAC1 (Mallory ...
... facilitating lateral root emergence. Furthermore NAC1 is targeted for degradation after ubiquitination through interaction with the E3-ligase SINAT5 (Xie et al., 2002). More recently, it was shown that the microRNA, miR164, targets several NAC-domain transcription factors, among which NAC1 (Mallory ...
Endogenous Drp1 Mediates Mitochondrial Autophagy and Protects
... After 1 hour of GD, the proportion of CMs with elongated mitochondria was increased, but that of CMs with foreshortened mitochondria was also increased slightly in Ad-shScr-transduced CMs (Figure 3C). A similar result was obtained in CMs transduced with adenovirus harboring LacZ (Ad-LacZ) (not shown ...
... After 1 hour of GD, the proportion of CMs with elongated mitochondria was increased, but that of CMs with foreshortened mitochondria was also increased slightly in Ad-shScr-transduced CMs (Figure 3C). A similar result was obtained in CMs transduced with adenovirus harboring LacZ (Ad-LacZ) (not shown ...
Mouse Hoxa2 mutations provide a model for microtia and auricle
... al., 2005; Schoenwolf and Larsen, 2009). Contrary to this prediction, we found that in E14.5 Wnt1::Cre;Hoxa2EGFP(lox-neo-lox)/+ mouse fetuses the EAC and its surrounding mesenchyme are entirely composed of Hoxa2–/EGFP– cells, and therefore not derived from the second arch (Fig. 1G,H,J). Moreover, we ...
... al., 2005; Schoenwolf and Larsen, 2009). Contrary to this prediction, we found that in E14.5 Wnt1::Cre;Hoxa2EGFP(lox-neo-lox)/+ mouse fetuses the EAC and its surrounding mesenchyme are entirely composed of Hoxa2–/EGFP– cells, and therefore not derived from the second arch (Fig. 1G,H,J). Moreover, we ...
Utilization and Transport of Mannitol in Olea
... europaea L., an important crop in the Mediterranean basin. Uptake of mannitol in heterotrophic cell suspensions of O. europaea was shown to be mediated by a 1 : 1 polyol : Hþ symport system with a Km of 1.3 mM mannitol and a Vmax of 1.3 nmol min1 mg1 DW. Dulcitol, sorbitol and xylitol competed for ...
... europaea L., an important crop in the Mediterranean basin. Uptake of mannitol in heterotrophic cell suspensions of O. europaea was shown to be mediated by a 1 : 1 polyol : Hþ symport system with a Km of 1.3 mM mannitol and a Vmax of 1.3 nmol min1 mg1 DW. Dulcitol, sorbitol and xylitol competed for ...
Running Head: Superresolution of cortical
... received limited attention in plant cell biology field (Fitzgibbon et al., 2010, Kleine-Vehn et al., ...
... received limited attention in plant cell biology field (Fitzgibbon et al., 2010, Kleine-Vehn et al., ...
Chapter 1: Overview - Worcester Polytechnic Institute
... admirable, and I thank you for that. I wish there were words to express my gratitude for supporting and guiding me throughout these past four years. I only hope that I have reached all your expectations and if not, you have me for the next ten years. These past few years you became more than an advi ...
... admirable, and I thank you for that. I wish there were words to express my gratitude for supporting and guiding me throughout these past four years. I only hope that I have reached all your expectations and if not, you have me for the next ten years. These past few years you became more than an advi ...
Materials and Methods
... was sampled, serially diluted and plated onto BHI Agar (Biolab) and the number of viable cells (CFU/ml) determined. At the same time 1 ml of the culture was centrifuged and the cell-free supernatant adjusted to pH 6.0 to 7.0 with 1 N sterile NaOH. The sample was concentrated ten-fold by freeze-dryin ...
... was sampled, serially diluted and plated onto BHI Agar (Biolab) and the number of viable cells (CFU/ml) determined. At the same time 1 ml of the culture was centrifuged and the cell-free supernatant adjusted to pH 6.0 to 7.0 with 1 N sterile NaOH. The sample was concentrated ten-fold by freeze-dryin ...
Micromechanical studies of mitotic chromosomes
... Fig. 1. Cell cycle in a newt cell. During mitosis, chromosomes condense inside the nucleus during prophase, the nuclear envelope disassembles and chromosomes float loose in the cytoplasm during prometaphase, they are captured and aligned by the spindle at metaphase, and the two duplicate chromatids o ...
... Fig. 1. Cell cycle in a newt cell. During mitosis, chromosomes condense inside the nucleus during prophase, the nuclear envelope disassembles and chromosomes float loose in the cytoplasm during prometaphase, they are captured and aligned by the spindle at metaphase, and the two duplicate chromatids o ...
Down-Regulates Expression of Cell Proliferation
... Special Fellows, and B.B.A. is a Ransom Horne, Jr., Professor of Cancer Research at The University of Texas M. D. Anderson Cancer Center. Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org. doi:10.1124/mol.105.017400. ...
... Special Fellows, and B.B.A. is a Ransom Horne, Jr., Professor of Cancer Research at The University of Texas M. D. Anderson Cancer Center. Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org. doi:10.1124/mol.105.017400. ...
Type XIII collagen. Structural and functional characterization of the
... trimerization of type XIII collagen chains was initiated by 21 amino acid residues adjacent to the transmembrane domain on the extracellular side, and this sequence was found to be conserved in several other collagenous transmembrane proteins. In addition to the transmembrane form, the ectodomain of ...
... trimerization of type XIII collagen chains was initiated by 21 amino acid residues adjacent to the transmembrane domain on the extracellular side, and this sequence was found to be conserved in several other collagenous transmembrane proteins. In addition to the transmembrane form, the ectodomain of ...
Physiology and molecular biology of petal senescence
... that more detailed analysis be carried out at the level of cells and organelles rather than at that of whole petals. ...
... that more detailed analysis be carried out at the level of cells and organelles rather than at that of whole petals. ...
Oxidative-stress-induced nuclear to cytoplasmic relocalization is
... Not4p. In this control, a modest amount of mono-, di- and triubiquitylated cyclin C was observed as well as some larger species at the stacker-gel–resolving-gel interface. These signals suggest that some residual activity exists in the in vitro transcription-coupled translation extracts but this act ...
... Not4p. In this control, a modest amount of mono-, di- and triubiquitylated cyclin C was observed as well as some larger species at the stacker-gel–resolving-gel interface. These signals suggest that some residual activity exists in the in vitro transcription-coupled translation extracts but this act ...
TEF30 interacts with photosystem II monomers and is involved in the
... PSII repair, while many more auxiliary factors play roles in PSII de novo synthesis and ...
... PSII repair, while many more auxiliary factors play roles in PSII de novo synthesis and ...
Expression, purification and characterization of azurin derived
... Finally, a special thank for my family for their inconditional support. And to my friends, especially my course colleagues for the relaxing lunch times. A special thank to João for always being there when I most needed. ...
... Finally, a special thank for my family for their inconditional support. And to my friends, especially my course colleagues for the relaxing lunch times. A special thank to João for always being there when I most needed. ...
Mechanisms for the Carcinostatic Activity of Se
... time and research efforts would make it clear that “the essential poison” was a part of several enzymes and proteins, found as the amino acid, selenocysteine. Within this same period of time, epidemiological evidence was also accumulating that a low dietary intake of selenium by humans may also be a ...
... time and research efforts would make it clear that “the essential poison” was a part of several enzymes and proteins, found as the amino acid, selenocysteine. Within this same period of time, epidemiological evidence was also accumulating that a low dietary intake of selenium by humans may also be a ...
Development of in vitro procedures that can predict the safety of
... pre-clinical safety tests that can better predict the toxicity of immunomodulatory mAbs, such as TGN1412, are required. The aim of this study was to investigate the hypothesis that cytokinedriven adverse effects of therapeutic monoclonal antibodies and the mechanisms involved can be better predicted ...
... pre-clinical safety tests that can better predict the toxicity of immunomodulatory mAbs, such as TGN1412, are required. The aim of this study was to investigate the hypothesis that cytokinedriven adverse effects of therapeutic monoclonal antibodies and the mechanisms involved can be better predicted ...
High-resolution imaging of cardiomyocyte behavior
... conduction system (Sedmera et al., 2003; Liu et al., 2010). Although studies in mouse have started to reveal the genetic basis of cardiac trabeculation, how cardiomyocytes form these important structures while continuously contracting remains relatively unknown. Most challenging for these in vivo st ...
... conduction system (Sedmera et al., 2003; Liu et al., 2010). Although studies in mouse have started to reveal the genetic basis of cardiac trabeculation, how cardiomyocytes form these important structures while continuously contracting remains relatively unknown. Most challenging for these in vivo st ...
A PP2A-B55 recognition signal controls substrate
... (-Gwl) inactivated conditions are shown in the graph. (B) Western blot analysis of PRC1 pT481 dephosphorylation in control (siControl), B55 (siB55)depleted, and Greatwall–MASTL (siMASTL)–depleted cell extracts. PRC1 pT481 level as a function of time expressed as integrated signal (It)/integrated s ...
... (-Gwl) inactivated conditions are shown in the graph. (B) Western blot analysis of PRC1 pT481 dephosphorylation in control (siControl), B55 (siB55)depleted, and Greatwall–MASTL (siMASTL)–depleted cell extracts. PRC1 pT481 level as a function of time expressed as integrated signal (It)/integrated s ...
Inositol trisphosphate and calcium oscillations
... The main feature of the luminal loading Ca2+ oscillation model is that the timing of each Ca2+ spike is determined by the periodic loading of the endoplasmic reticulum with Ca2+ (Figure 1). The best way of describing how this oscillator might function is to follow what happens when cells are stimula ...
... The main feature of the luminal loading Ca2+ oscillation model is that the timing of each Ca2+ spike is determined by the periodic loading of the endoplasmic reticulum with Ca2+ (Figure 1). The best way of describing how this oscillator might function is to follow what happens when cells are stimula ...
Regulation of epidermal growth factor receptor signalling by
... signalling. Four such IFIs have been identified so far in mammals, namely leucine-rich and immunoglobulin-like domains protein 1 (LRIG1) (Gur et al., 2004; Laederich et al., 2004), suppressor of cytokine signalling 4 and 5 (SOCS4 and SOCS5) (Nicholson et al., 2005; Kario et al., 2005) and receptor-a ...
... signalling. Four such IFIs have been identified so far in mammals, namely leucine-rich and immunoglobulin-like domains protein 1 (LRIG1) (Gur et al., 2004; Laederich et al., 2004), suppressor of cytokine signalling 4 and 5 (SOCS4 and SOCS5) (Nicholson et al., 2005; Kario et al., 2005) and receptor-a ...
FGF signaling is essential for the early events in the development of
... the cells invaginating through the primitive streak and in the mesodermal cells that had traversed through the primitive streak and placed adjacent to it (Fig. 5 B-D). Few endodermal cells were also found to express Brachyury (Fig. 5B). In bFGF and suramin treated embryos, the spatial distribution o ...
... the cells invaginating through the primitive streak and in the mesodermal cells that had traversed through the primitive streak and placed adjacent to it (Fig. 5 B-D). Few endodermal cells were also found to express Brachyury (Fig. 5B). In bFGF and suramin treated embryos, the spatial distribution o ...
Stochastic and reversible aggregation of mRNA with expanded CUG
... Previous studies have shown that one can measure the dynamics of the MS2-tagged RNA itself, and not the exchange rate of the MS2–GFP molecule on the MS2 stem-loop, by using FRAP (Boireau et al., 2007; Mollet et al., 2008; Shav-Tal et al., 2004). With this system, we were thus able to perform FRAP an ...
... Previous studies have shown that one can measure the dynamics of the MS2-tagged RNA itself, and not the exchange rate of the MS2–GFP molecule on the MS2 stem-loop, by using FRAP (Boireau et al., 2007; Mollet et al., 2008; Shav-Tal et al., 2004). With this system, we were thus able to perform FRAP an ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.