Capture of AT-rich Chromatin by ELYS Recruits POM121 and NDC1
... The vertebrate protein ELYS has been shown to play the earliest known role in initiating and targeting nuclear pore assembly to the chromatin (Rasala et al., 2006; Franz et al., 2007). Mutations in MEL-28, the Caenorhabditis elegans homologue of ELYS, show clear defects in nuclear envelope morpholog ...
... The vertebrate protein ELYS has been shown to play the earliest known role in initiating and targeting nuclear pore assembly to the chromatin (Rasala et al., 2006; Franz et al., 2007). Mutations in MEL-28, the Caenorhabditis elegans homologue of ELYS, show clear defects in nuclear envelope morpholog ...
Post-transcriptional regulation of gene expression in innate immunity
... is subject to extensive post-transcriptional regulation, in which more than 256 alternatively processed transcripts encode variants of receptors, adaptors and signalling molecules13. Every TLR gene has numerous alternatively spliced variants13–18, and TLR1 to TLR7 all have between two and four predi ...
... is subject to extensive post-transcriptional regulation, in which more than 256 alternatively processed transcripts encode variants of receptors, adaptors and signalling molecules13. Every TLR gene has numerous alternatively spliced variants13–18, and TLR1 to TLR7 all have between two and four predi ...
y. Cell Set. Suppl. ¡1, 1-11 (1989) Printed in
... of contact between the two mitochondrial membranes, we combined genetic engineering and chemical crosslinking techniques to produce a chimeric mitochon drial precursor protein which became stuck in the protein import m achinery (Vestweber & Schatz, 1988a). To construct this chimeric protein, we fir ...
... of contact between the two mitochondrial membranes, we combined genetic engineering and chemical crosslinking techniques to produce a chimeric mitochon drial precursor protein which became stuck in the protein import m achinery (Vestweber & Schatz, 1988a). To construct this chimeric protein, we fir ...
University of Groningen How to get (a)round Pinho, Mariana
... and generate two equally sized daughter cells (FIG. 1a). Little is known about cell wall remodelling in cocci, but cryo-electron microscopy of thin sections of S. aureus cells has shown that complete septa are composed of a low-density zone that separates two zones of high density which correspond t ...
... and generate two equally sized daughter cells (FIG. 1a). Little is known about cell wall remodelling in cocci, but cryo-electron microscopy of thin sections of S. aureus cells has shown that complete septa are composed of a low-density zone that separates two zones of high density which correspond t ...
Imaging the fate of histone Cse4 reveals de novo replacement in S
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
Determinants of Drosophila zw10 protein localization and function
... have found that the zw10 protein localizes to the region of the kinetochores in cells mitotically arrested by either colchicine or taxol treatment. Second, we demonstrate that PSCS in mitotically arrested zw10 mutant cells occurs in the absence of cyclin B degradation. This supports the recent findi ...
... have found that the zw10 protein localizes to the region of the kinetochores in cells mitotically arrested by either colchicine or taxol treatment. Second, we demonstrate that PSCS in mitotically arrested zw10 mutant cells occurs in the absence of cyclin B degradation. This supports the recent findi ...
Functional Anthology of Intrinsic Disorder. 1. Biological Processes
... view of intrinsic disorder.6 More detailed analysis of extended disordered proteins/regions revealed that they can be further divided into two groups, random coil-like and pre-molten globule-like conformations.7 Recently, more than 150 proteins have been identified as containing functional disordere ...
... view of intrinsic disorder.6 More detailed analysis of extended disordered proteins/regions revealed that they can be further divided into two groups, random coil-like and pre-molten globule-like conformations.7 Recently, more than 150 proteins have been identified as containing functional disordere ...
FLASH CARDS
... The diffusion of particles through proteins in the cell membranes from areas where the concentration of particles is high to areas where the concentration of particles is low ...
... The diffusion of particles through proteins in the cell membranes from areas where the concentration of particles is high to areas where the concentration of particles is low ...
Molecular organization of the cell wall of Candida albicans
... of isolated cell walls released proteins that were crossreactive with an anti-Als serum, suggesting that C. dubliniensis and C. tropicalis have similar cell-wall architecture as C. albicans and S. cerevisiae. Finally, a putative GPI-CWP, EPA1, encoding an adhesion protein, has been identi ed in C. ...
... of isolated cell walls released proteins that were crossreactive with an anti-Als serum, suggesting that C. dubliniensis and C. tropicalis have similar cell-wall architecture as C. albicans and S. cerevisiae. Finally, a putative GPI-CWP, EPA1, encoding an adhesion protein, has been identi ed in C. ...
L 9 Myosin
... ion concentration within the muscle cell. • A component of the troponin complex senses the increase in calcium and, in response, relieves the inhibition of myosin - actin interactions by ...
... ion concentration within the muscle cell. • A component of the troponin complex senses the increase in calcium and, in response, relieves the inhibition of myosin - actin interactions by ...
The Cell Membrane
... The inhibitors are often classified as competitive and non competitive. If an inhibitor binds irreversibly to the carrier, leaving no chance for the substrate to compete for a place on the carrier, the inhibition is called noncompetitive. The carrier in effect gets inactivated. If, however, the inh ...
... The inhibitors are often classified as competitive and non competitive. If an inhibitor binds irreversibly to the carrier, leaving no chance for the substrate to compete for a place on the carrier, the inhibition is called noncompetitive. The carrier in effect gets inactivated. If, however, the inh ...
Single Cell Analysis of RNA-mediated Histone H3.3 Transcription Site
... high throughput sequencing; PML, promyelocytic leukemia; NB, nuclear body. ...
... high throughput sequencing; PML, promyelocytic leukemia; NB, nuclear body. ...
Intracellular localization of NDH II - Journal of Cell Science
... for hnRNP A1 (Fig. 1A′). hnRNP A1 undergoes relocalization into the cytoplasm when transcription is inhibited (Piñol-Roma and Dreyfuss, 1992). A similar effect was also observed for NDH II after treatment of cells with actinomycin D (Fig. 1B). However, following transcriptional inhibition the increa ...
... for hnRNP A1 (Fig. 1A′). hnRNP A1 undergoes relocalization into the cytoplasm when transcription is inhibited (Piñol-Roma and Dreyfuss, 1992). A similar effect was also observed for NDH II after treatment of cells with actinomycin D (Fig. 1B). However, following transcriptional inhibition the increa ...
Becke S, Fabre-Mersseman V, Aue S, Auerochs S
... a complex with the viral RNA-export protein pUL69 and with pUL97 in lysates of infected cells. No evidence for an impairment of pUL97 within this complex was found. However, RV-VM1 replication in infected cells was resistant to a pUL97 inhibitor, and pUL97 inhibitors mimicked the mutant in terms of ...
... a complex with the viral RNA-export protein pUL69 and with pUL97 in lysates of infected cells. No evidence for an impairment of pUL97 within this complex was found. However, RV-VM1 replication in infected cells was resistant to a pUL97 inhibitor, and pUL97 inhibitors mimicked the mutant in terms of ...
Polarity and endocytosis: reciprocal regulation
... analyses of Rab11-depleted conditions are hampered by the general requirement of Rab11 for cell viability in Drosophila, and also complicated by its additional role in polarized biosynthetic delivery pathways [28,29]. The polarity phenotypes associated with loss of ESCRT proteins, which would be exp ...
... analyses of Rab11-depleted conditions are hampered by the general requirement of Rab11 for cell viability in Drosophila, and also complicated by its additional role in polarized biosynthetic delivery pathways [28,29]. The polarity phenotypes associated with loss of ESCRT proteins, which would be exp ...
The integrin–actin connection, an eternal love affair
... controls integrin function. In CHO cells, increased ®lamin binding to integrins by the introduction of point mutations into the cytoplasmic domain of b1 and b7-integrins reduced membrane protrusion, cell polarization and consequently cell migration (Calderwood et al., 2001). Filamin can induce reorg ...
... controls integrin function. In CHO cells, increased ®lamin binding to integrins by the introduction of point mutations into the cytoplasmic domain of b1 and b7-integrins reduced membrane protrusion, cell polarization and consequently cell migration (Calderwood et al., 2001). Filamin can induce reorg ...
Brain days-Part III
... A station where much of the information is integrated and relayed to the cerebral cortex and many other subcortical regions. A key role in the integration of visceral and somatic functions. ...
... A station where much of the information is integrated and relayed to the cerebral cortex and many other subcortical regions. A key role in the integration of visceral and somatic functions. ...
Epithelial differentiation and intercellular junction
... cumulus cell processes and the oocyte surface where uvomorulin (Vestweber et al., 1987), and gap and desmosome junctions (Anderson and Albertini, 1976) have been identified previously. Indeed, cingulin is detectable at both the cumulus and oocyte sides of this contact site in ovarian preovulatory fo ...
... cumulus cell processes and the oocyte surface where uvomorulin (Vestweber et al., 1987), and gap and desmosome junctions (Anderson and Albertini, 1976) have been identified previously. Indeed, cingulin is detectable at both the cumulus and oocyte sides of this contact site in ovarian preovulatory fo ...
Anatomy and Physiology of Headache Biomedicine
... 26) Nociception pain can be caused by mechanical stimulation following a “distortion of a network of collagen” such as ligament or dura mater. [Important: this supports the mechanics of the subluxation] 27) Central pain involves no tissue damage but results from dysfunction of the descending pain in ...
... 26) Nociception pain can be caused by mechanical stimulation following a “distortion of a network of collagen” such as ligament or dura mater. [Important: this supports the mechanics of the subluxation] 27) Central pain involves no tissue damage but results from dysfunction of the descending pain in ...
cells
... • A large cell cannot move material in & out fast enough to support life • How to get bigger?? Become multicellular! ...
... • A large cell cannot move material in & out fast enough to support life • How to get bigger?? Become multicellular! ...
Prm1p, a Pheromone-regulated Multispanning Membrane Protein
... Jahn and Sudhof, 1999). Although hemagglutinin and SNAREs differ in composition—hemagglutinin is a single viral surface protein capable of inserting directly into the host cell plasma membrane, whereas the SNARE complex assembles from subunits associated with different bilayers— their final structur ...
... Jahn and Sudhof, 1999). Although hemagglutinin and SNAREs differ in composition—hemagglutinin is a single viral surface protein capable of inserting directly into the host cell plasma membrane, whereas the SNARE complex assembles from subunits associated with different bilayers— their final structur ...
Cell nucleus
In cell biology, the nucleus (pl. nuclei; from Latin nucleus or nuculeus, meaning kernel) is a membrane-enclosed organelle found in eukaryotic cells. Eukaryotes usually have a single nucleus, but a few cell types have no nuclei, and a few others have many.Cell nuclei contain most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these chromosomes are the cell's nuclear genome. The function of the nucleus is to maintain the integrity of these genes and to control the activities of the cell by regulating gene expression—the nucleus is, therefore, the control center of the cell. The main structures making up the nucleus are the nuclear envelope, a double membrane that encloses the entire organelle and isolates its contents from the cellular cytoplasm, and the nucleoskeleton (which includes nuclear lamina), a network within the nucleus that adds mechanical support, much like the cytoskeleton, which supports the cell as a whole.Because the nuclear membrane is impermeable to large molecules, nuclear pores are required that regulate nuclear transport of molecules across the envelope. The pores cross both nuclear membranes, providing a channel through which larger molecules must be actively transported by carrier proteins while allowing free movement of small molecules and ions. Movement of large molecules such as proteins and RNA through the pores is required for both gene expression and the maintenance of chromosomes. The interior of the nucleus does not contain any membrane-bound sub compartments, its contents are not uniform, and a number of sub-nuclear bodies exist, made up of unique proteins, RNA molecules, and particular parts of the chromosomes. The best-known of these is the nucleolus, which is mainly involved in the assembly of ribosomes. After being produced in the nucleolus, ribosomes are exported to the cytoplasm where they translate mRNA.