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–plasma-membrane junctions by Kv2.1 Induction of stable ER potassium channels
–plasma-membrane junctions by Kv2.1 Induction of stable ER potassium channels

... ER–plasma-membrane junctions, or sub-surface cisterns (SSCs), as demonstrated by more than 50 years of electron microscopy experiments (Rosenbluth, 1962), little is known about the molecular components involved in neuronal ER–plasma-membrane junction formation, maintenance and function (Helle et al. ...
Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism
Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism

Eucalyptus grandis cellulose synthase 1 Arabidopsis thaliana MARJA M. O’NEILL
Eucalyptus grandis cellulose synthase 1 Arabidopsis thaliana MARJA M. O’NEILL

... mostly contained in secondary cell walls, with cellulose forming the most important component for paper and pulp manufacturers. Cellulose is a cell wall polymer of chains of β1,4-linked glucose molecules that crystallize to form cellulose microfibrils in plant cell walls. Biosynthesis of cellulose i ...
Actin Interacting Protein1 and Actin Depolymerizing
Actin Interacting Protein1 and Actin Depolymerizing

Failsafe Mechanisms Coordinate Cell Division and the Initiation of
Failsafe Mechanisms Coordinate Cell Division and the Initiation of

... DNA  replication  termination  to  division  (the  “D  period,”  analogous  to  mitosis  and   cytokinesis  in  eukaryotes)  (Figure  2A)  [3].       This  view  of  the  cell  cycle  is  complicated  when  cells  are  grown  in  rich ...
Leukocyte lipid bodies — Biogenesis and functions in inflammation
Leukocyte lipid bodies — Biogenesis and functions in inflammation

... bodies was to have these membrane-active proteins “translocate” to the peripheral membrane of lipid bodies. Of note, however, immunogold electron microscopic localization of 5-LO revealed 5-LO to be distributed throughout lipid bodies, which were shown to have a “honeycomb” internal structure [39], ...
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin 2
Localization of retinitis pigmentosa 2 to cilia is regulated by Importin 2

... trafficked to the cilia is unknown. Here we have characterized an interaction between RP2 and Importin 2 (transportin-1), a member of the Importin- family that regulates nuclear–cytoplasmic shuttling. We demonstrate that Importin 2 is necessary for localization of RP2 to the primary cilium becaus ...
Bacillus cereus
Bacillus cereus

... that is able to produce a variety of proteins and sources of bioremediation. Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. This species is good cloning host because it is able to house numerous plasm ...
Mathematical Biology
Mathematical Biology

... PMN’s can exhibit forward protrusion as rapid as 30µ/min (Zigmond, 1993). Other typical rates of protrusion include 2.4µ/min in fish keratocytes, and as low as 0.4µ/min, 0.08µ/min in (respectively) human and mouse fibroblasts (Theriot, 1994). The half-life of F-actin in cells has been observed to be ...
Interaction of PIN and PGP transport mechanisms in auxin
Interaction of PIN and PGP transport mechanisms in auxin

... Transport-based asymmetric auxin distribution within tissues (auxin gradients) plays an important role in many developmental processes, including patterning and tropisms (reviewed by Tanaka et al., 2006). Because the auxin molecule is charged inside cells and, thus, membrane impermeable, its interce ...
HELICOBACTER PYLORI VacA, A PARADIGM FOR TOXIN
HELICOBACTER PYLORI VacA, A PARADIGM FOR TOXIN

... cause the formation of large intracellular VACUOLES in cultured mammalian cells14 (FIG. 2a). The H. pylori protein responsible for this effect (designated vacuolating cytotoxin or VacA) is encoded by a chromosomal gene known as vacA15–18 (FIG. 3). The amino acid sequence of VacA does not show simila ...
Funguslike Protists
Funguslike Protists

... 12. Protozoans that are __________________________ feed on the cells and body fluids of their hosts. 13. Is the following sentence true or false? Protozoans that are parasites never have more than one host. __________________________________________________________ ...
Elevated Level of Nuclear Protein Kinase C in
Elevated Level of Nuclear Protein Kinase C in

... specific for the o, ß,and 7 isotypes of PKC prepared as described in "Materials and Methods." In intact MCF-7 cells, multidrug-resistant MCF-7/ADR cells showed a markedly en hanced immunoreactivity to PKC compared to that of drugsensitive MCF-7/WT cells (Fig. 1). The most pronounced difference was ...
Live cell imaging reveals actin-cytoskeleton
Live cell imaging reveals actin-cytoskeleton

... actin-binding activity in vitro (Thomas et al., 2007). It is therefore possible that plant LIMs crosslink actin filaments in a monomeric form, although dimerization or oligomerization cannot be ruled out. Indeed, a recent study focusing on the nuclear functions of plant LIM proteins suggested that t ...
Proper Folding and Endoplasmic Reticulum to Golgi
Proper Folding and Endoplasmic Reticulum to Golgi

... charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. § To whom correspondence should be addressed: Dept. of Dermatology, Yale University School of Medicine, P. O. Box 208059, 15 York St., ...
Cellular Auxin Transport in Algae
Cellular Auxin Transport in Algae

... relatives to the land plants. In this division, the order Charales has been widely accepted as a sister-relationship to the land plants over the past century, which was based also on the morphology since the Charales develop the most complicated, land-plant-like body structures as compared to all th ...
Roles of Arabidopsis PARC6 in Coordination of
Roles of Arabidopsis PARC6 in Coordination of

... region of PARC6 or a region overlapping with both PARC677-356 and PARC6357-573 is required. To further analyze the interactions between PARC6 and the FtsZ proteins, we fused mCherry to the stromal region of PARC6 to create PARC677-573-mCherry and coexpressed it with either FtsZ1-eYFP (enhanced yello ...
Depletion of the co-chaperone CDC-37 reveals two
Depletion of the co-chaperone CDC-37 reveals two

... acts by maintaining the cyclin-dependent kinase Cdc28p in a state that is competent to associate with G1 and mitotic cyclins (Gerber et al., 1995). Vertebrate Cdc37 homologs have been identified in a number of studies as ~50 kDa proteins that associate with Hsp90 and a variety of other proteins, par ...
The Cutting Edge of Affinity Electrophoresis Technology
The Cutting Edge of Affinity Electrophoresis Technology

... compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cells. It has been reported that changes in the structures of the sugar chains of mucins a ...
a va i l a b l e a t... w w w. e l s e v i e...
a va i l a b l e a t... w w w. e l s e v i e...

... evolved [7,8,10]. However, a nuclear function for Delta in a relevant biological context remains to be demonstrated and thus it is uncertain if Delta is a substrate for a classical RIP mechanism. Despite recent advancements, the mechanism of Delta cleavage is not completely understood. Drosophila De ...
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain

The Larynx
The Larynx

...  The larynx constitutes the organ of phonation ...
Masking the Pathogen: Evolutionary Strategies of Fungi and Their
Masking the Pathogen: Evolutionary Strategies of Fungi and Their

Flamingo controls the planar polarity of sensory
Flamingo controls the planar polarity of sensory

... apterous–Gal4 also disrupted bristle planar polarity (Figure 2b), consistent with earlier experiments using fz constructs driven by the heat shock gene (hs) promoter [8]. Besides the planar polarity phenotype, overexpression of fz also led to the formation of ectopic macrochaetes. The apterous–Gal4 ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and

... The two major lupin storage proteins are α-conglutin (legumin-like or 11S globulin), and ß-conglutin (vicilin-like or 7S globulin). Vilicin proteins are characterized by two cupin (barrel-shaped) domains constituted by α-helices. Another family with a cupin-like structure, γ-conglutin (basic 7S-glob ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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