Universitas Scientiarum
... specialized equipment may be restrictive. Some techniques to preserve bacteria such as freeze-drying and cryopreservation may maintain cells viable for many years; however, these techniques may also cause severe damages to bacterial cells (Miyamoto-Shinohara et al. 2000). Under optimal conditions ce ...
... specialized equipment may be restrictive. Some techniques to preserve bacteria such as freeze-drying and cryopreservation may maintain cells viable for many years; however, these techniques may also cause severe damages to bacterial cells (Miyamoto-Shinohara et al. 2000). Under optimal conditions ce ...
Genome-wide gene expression in uro mutant
... the uro hypocotyl is severely blocked, resulting in reduced numbers of vascular cells and much less accumulated lignin in the secondary cell walls of both hypocotyl and stem. Cellulose is known to be another important composition in forming secondary cell walls. In addition to lignin, cellulose cont ...
... the uro hypocotyl is severely blocked, resulting in reduced numbers of vascular cells and much less accumulated lignin in the secondary cell walls of both hypocotyl and stem. Cellulose is known to be another important composition in forming secondary cell walls. In addition to lignin, cellulose cont ...
Quantitative analysis of changes in spatial distribution and plus
... daughter nuclei (n). The growing cell plate (cp) is surrounded by many vesicles and is sandwiched between the two sets of microtubules. (D) Transitionalphragmoplast stage (2.8⫻2.8⫻0.8 m), which lacks a cell-plate assembly matrix and displays many short microtubules on both sides of the maturing cel ...
... daughter nuclei (n). The growing cell plate (cp) is surrounded by many vesicles and is sandwiched between the two sets of microtubules. (D) Transitionalphragmoplast stage (2.8⫻2.8⫻0.8 m), which lacks a cell-plate assembly matrix and displays many short microtubules on both sides of the maturing cel ...
The variability of autophagy and cell death susceptibility
... involving lysosomal limiting membrane invagination, protrusion or septation. Macroautophagy involves a dynamic subcellular membrane rearrangement whereby cytoplasmic proteins and organelles are sequestered in double-membrane vesicular structures (autophagosomes) that fuse with the lysosome or vacuol ...
... involving lysosomal limiting membrane invagination, protrusion or septation. Macroautophagy involves a dynamic subcellular membrane rearrangement whereby cytoplasmic proteins and organelles are sequestered in double-membrane vesicular structures (autophagosomes) that fuse with the lysosome or vacuol ...
Enzymatic lysis of microbial cells
... one or more b(1 fi 3) glucanase (lytic and nonlytic), protease, b(1 fi 6) glucanase, mannanase and chitinase, which act synergistically for lysing the cell wall. Enzymatic cell lysis of yeast begins with binding of the lytic protease to the outer mannoprotein layer of the wall. The protease opens up t ...
... one or more b(1 fi 3) glucanase (lytic and nonlytic), protease, b(1 fi 6) glucanase, mannanase and chitinase, which act synergistically for lysing the cell wall. Enzymatic cell lysis of yeast begins with binding of the lytic protease to the outer mannoprotein layer of the wall. The protease opens up t ...
Endoplasmic Reticulum-Localized Amyloid β
... Budding of transport vesicles from the microsomes was unlikely under the experimental conditions used here. Nevertheless, the possibility that the Ab42 in the supernatant was contained within transport vesicles and not translocated across the microsomal membrane was analyzed by protease protection a ...
... Budding of transport vesicles from the microsomes was unlikely under the experimental conditions used here. Nevertheless, the possibility that the Ab42 in the supernatant was contained within transport vesicles and not translocated across the microsomal membrane was analyzed by protease protection a ...
Characterization and Biological Activities of Ocellatin Peptides from
... -PT2) decreases the α-helix content (Figures S4 and S5A). This could be related to antimicrobial activity against E. coli, as peptides with Phe3 and Gly8 present slightly lower MICs against this strain (Table 3). The presence of the amino acids Asp8 (in ocellatins-PT1, -PT2, and -PT-5), Asp12 (ocell ...
... -PT2) decreases the α-helix content (Figures S4 and S5A). This could be related to antimicrobial activity against E. coli, as peptides with Phe3 and Gly8 present slightly lower MICs against this strain (Table 3). The presence of the amino acids Asp8 (in ocellatins-PT1, -PT2, and -PT-5), Asp12 (ocell ...
INVESTIGATION OF THE EFFECTOR ROLE OF
... species: S. dysenteriae, S. flexneri, S. boydii and S. sonnei (also known as serogroups A through D). All species share approximately 80-85% of their genome33, and have been distinctly classified as a separate clade from Escherichia coli despite their phylogenetic closeness34. Variations in the O-an ...
... species: S. dysenteriae, S. flexneri, S. boydii and S. sonnei (also known as serogroups A through D). All species share approximately 80-85% of their genome33, and have been distinctly classified as a separate clade from Escherichia coli despite their phylogenetic closeness34. Variations in the O-an ...
Redistribution of membrane proteins between the Golgi apparatus
... apparatus by the addition of targeting regions of Golgi transferases (Boevink et al., 1998; Essl et al., 1999) or complete enzymes (NebenfuÈhr et al., 1999) has demonstrated that in suspension culture cells, leaf epidermal cells, hypocotyls, and roots (Saint-Jore, Moore and Hawes unpublished) indivi ...
... apparatus by the addition of targeting regions of Golgi transferases (Boevink et al., 1998; Essl et al., 1999) or complete enzymes (NebenfuÈhr et al., 1999) has demonstrated that in suspension culture cells, leaf epidermal cells, hypocotyls, and roots (Saint-Jore, Moore and Hawes unpublished) indivi ...
Methyl Jasmonate-Induced Ethylene Production
... lumen size (Fig. 1B). The radial ducts of the secondary phloem were often seen to be connected with the induced xylem TDs (Fig. 1B). These same responses were found at all four internodes from which sections were collected, although the response was reduced in magnitude at both internodes above the ...
... lumen size (Fig. 1B). The radial ducts of the secondary phloem were often seen to be connected with the induced xylem TDs (Fig. 1B). These same responses were found at all four internodes from which sections were collected, although the response was reduced in magnitude at both internodes above the ...
Full version (PDF file)
... analysis of the biological behavior of certain nanotherapeutics due to their intrinsic fluorescence. These methods are applicable for evaluating the drug release profile, localization, trafficking, changes in pH, local viscosity and the presence of quencher (Calleja et al. 2003, Rihova et al. 2010, ...
... analysis of the biological behavior of certain nanotherapeutics due to their intrinsic fluorescence. These methods are applicable for evaluating the drug release profile, localization, trafficking, changes in pH, local viscosity and the presence of quencher (Calleja et al. 2003, Rihova et al. 2010, ...
Sarcomere assembly in C. elegans muscle
... membrane-proximal adaptors, but lack vinculin. The membrane-distal region of the M-line lacks the dense body protein alpha-actinin, but does include the M-line specific protein UNC-89 (Benian et al., 1996). Given their protein composition and functions, dense bodies and M-lines are both analogous an ...
... membrane-proximal adaptors, but lack vinculin. The membrane-distal region of the M-line lacks the dense body protein alpha-actinin, but does include the M-line specific protein UNC-89 (Benian et al., 1996). Given their protein composition and functions, dense bodies and M-lines are both analogous an ...
Filamentous Influenza Virus Enters Cells via
... showed that M2 is able to alter membrane curvature in a cholesterol-dependent manner, a property that is essential for the for- ...
... showed that M2 is able to alter membrane curvature in a cholesterol-dependent manner, a property that is essential for the for- ...
Detection and localization of single molecular recognition
... atomic force microscope (AFM) has emerged as a powerful tool for exploring the forces and the dynamics of the interaction between individual ligands and receptors, either on isolated molecules or on cellular surfaces. These studies require attaching specific biomolecules or cells on AFM tips and on ...
... atomic force microscope (AFM) has emerged as a powerful tool for exploring the forces and the dynamics of the interaction between individual ligands and receptors, either on isolated molecules or on cellular surfaces. These studies require attaching specific biomolecules or cells on AFM tips and on ...
Cytochrome c Release and Apoptosis Induced by Mitochondrial
... The orphan receptor TR3 (also known as nur77 or nerve growth factor–induced clone B NGFI-B) (1–3) functions as a nuclear transcription factor in the regulation of target gene expression (4–7). TR3 was originally isolated as an immediate-early gene rapidly expressed in response to serum or phorbol es ...
... The orphan receptor TR3 (also known as nur77 or nerve growth factor–induced clone B NGFI-B) (1–3) functions as a nuclear transcription factor in the regulation of target gene expression (4–7). TR3 was originally isolated as an immediate-early gene rapidly expressed in response to serum or phorbol es ...
Six Distinct Nuclear Factors Interact with the 75-Base
... enhancer. This became clear when fragment I was used because a multiplicity of protein-DNA complexes was observed (Fig. 2, lane 1). These bands could be partially resolved by chromatographically fractionating the WEHI 231 nuclear extract through heparin-agarose or phosphocellulose by using a three-s ...
... enhancer. This became clear when fragment I was used because a multiplicity of protein-DNA complexes was observed (Fig. 2, lane 1). These bands could be partially resolved by chromatographically fractionating the WEHI 231 nuclear extract through heparin-agarose or phosphocellulose by using a three-s ...
Branching morphogenesis of the Drosophila tracheal system. Annual Review of Cell Developmental Biology 19, 623-647. pdf
... events showed that there are surprisingly diverse ways of forming tracheal tubes and that a different tubulogenesis mechanism is used at each level of branching; branching is thus not a strictly reiterative process (Samakovlis et al. 1996a). The tracheal sacs are the first tube-like structures to fo ...
... events showed that there are surprisingly diverse ways of forming tracheal tubes and that a different tubulogenesis mechanism is used at each level of branching; branching is thus not a strictly reiterative process (Samakovlis et al. 1996a). The tracheal sacs are the first tube-like structures to fo ...
Coupling of thraustochytrids and POM, and of bacterio
... the Seto Inland Sea, Japan, with reference to concentrations of potential growth substrates such as dissolved organic carbon (DOC) and particulate organic carbon (POC) concentration and of phytoplankton (chlorophyll a concentration). Thraustochytrid and bacterioplankton cells were stained with acrif ...
... the Seto Inland Sea, Japan, with reference to concentrations of potential growth substrates such as dissolved organic carbon (DOC) and particulate organic carbon (POC) concentration and of phytoplankton (chlorophyll a concentration). Thraustochytrid and bacterioplankton cells were stained with acrif ...
Extracellular O2 Probe
... Antimycin) and uncoupling (FCCP) being detected. Despite this dysfunction and an additional 24 hour exposure, analysis of cellular ATP concentrations indicated high levels of ‘viability’ (Fig. 2B). This pattern is also reflected using other assays and cell lines. 3.6. Extracellular O2 Probe: compari ...
... Antimycin) and uncoupling (FCCP) being detected. Despite this dysfunction and an additional 24 hour exposure, analysis of cellular ATP concentrations indicated high levels of ‘viability’ (Fig. 2B). This pattern is also reflected using other assays and cell lines. 3.6. Extracellular O2 Probe: compari ...
Treatment of acute promyelocytic leukaemia with all
... South Chongqing Road, Shanghai 200025, People’s Republic of China To turn a disease from highly fatal to highly curable is extremely difficult, especially when the disease is a type of cancer. However, we can gain some insight into how this can be done by looking back over the 50-year history of tam ...
... South Chongqing Road, Shanghai 200025, People’s Republic of China To turn a disease from highly fatal to highly curable is extremely difficult, especially when the disease is a type of cancer. However, we can gain some insight into how this can be done by looking back over the 50-year history of tam ...
the fine-tuning of the endomembrane system
... genetics. Symbiosis is based on the fine-tuning of host cell and bacteria that ensure the co-existence and mutual benefit. Such processes involve the reorganization of endomembrane system in infected cells that have to be studied by the methods of plant biology and molecular biology. The morphology ...
... genetics. Symbiosis is based on the fine-tuning of host cell and bacteria that ensure the co-existence and mutual benefit. Such processes involve the reorganization of endomembrane system in infected cells that have to be studied by the methods of plant biology and molecular biology. The morphology ...
The role of yolk syncytial layer and blastoderm movements during
... colleagues, who began genetic analysis in the zebrafish and established the methodological foundation allowing its use as a vertebrate model organism (Streisinger et al., 1981; Westerfield, 2000). Indeed, the zebrafish embryo offers many advantages to study embryonic development compared to other or ...
... colleagues, who began genetic analysis in the zebrafish and established the methodological foundation allowing its use as a vertebrate model organism (Streisinger et al., 1981; Westerfield, 2000). Indeed, the zebrafish embryo offers many advantages to study embryonic development compared to other or ...
Comparing The Effects of Small Molecules BIX
... 50-fold for three-factor (Oct4, Sox2 and Klf4) and four-factor (Oct4, Sox2, Klf4 and c-Myc) reprogramming respectively. VPA enhances reprogramming efficiency and consequently provides a chance for reducing the number of required reprogramming factors. In the presence of VPA, the three-factor-infecte ...
... 50-fold for three-factor (Oct4, Sox2 and Klf4) and four-factor (Oct4, Sox2, Klf4 and c-Myc) reprogramming respectively. VPA enhances reprogramming efficiency and consequently provides a chance for reducing the number of required reprogramming factors. In the presence of VPA, the three-factor-infecte ...
in vivo chromatin structures
... Restriction Endonuclease Cleavage Sites in SV40 DNA Accessible. Free SV40 DNA (control) and HCHO-fixed, Pronasedigested chromosomes were limit-digested with various restriction endonucleases followed by complete thermal reversal ofthe Pronase-resistant crosslinks and gel electrophoresis (Fig. 3A). T ...
... Restriction Endonuclease Cleavage Sites in SV40 DNA Accessible. Free SV40 DNA (control) and HCHO-fixed, Pronasedigested chromosomes were limit-digested with various restriction endonucleases followed by complete thermal reversal ofthe Pronase-resistant crosslinks and gel electrophoresis (Fig. 3A). T ...