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4. Fertilize eggs as described in the basic protocol
4. Fertilize eggs as described in the basic protocol

... form spicules, the urchin skeleton (Figure 1). Then, the vegetal plate invaginates, forming the archenteron, and this archenteron migrates up the sea urchin’s blastocoel wall with the help of secondary mesenchyme cells (Figure 1). The migration of the archenteron depends not only on signals and prot ...
Smad5 determines murine amnion fate through the
Smad5 determines murine amnion fate through the

... Fig. 1. Impaired allantois and PGC development in Smad5m1/m1 embryos. (A-C) Allantois dimensions measured in embryos collected from Smad5m1/+ crosses in F2 (C57BL/6J ⫻ CBA) background. Results at different developmental stages were expressed as means ± s.e. The numbers under the histogram represent ...
Incidence of Epstein–Barr virus in Syrian women with breast cancer
Incidence of Epstein–Barr virus in Syrian women with breast cancer

... initiation and progression of human malignancies including several types of lymphoma and carcinomas. Several recent studies reported that approximately 30–50% of human breast cancers, worldwide, are positive for EBV;11-15 controversially a few studies revealed that EBV could not be detected in human ...
Deletion of Lipoteichoic Acid Synthase Impacts
Deletion of Lipoteichoic Acid Synthase Impacts

L2 Inflam 20162016-10-24 22:226.6 MB
L2 Inflam 20162016-10-24 22:226.6 MB

ATR Regulates a G2-Phase Cell-Cycle Checkpoint
ATR Regulates a G2-Phase Cell-Cycle Checkpoint

... mutants, resulting in compaction of nuclei and subsequent cell death. By contrast, HU-treated wild-type and atr plants arrested in G1 and showed no obvious signs of cell death. We propose that, in plants, HU invokes a novel checkpoint responsive to low levels of deoxynucleotide triphosphates. These ...
Tubular secretion
Tubular secretion

... Business  Homework due in lab  TASS W/F 12pm  Contact the Tutoring Center for more details  BI 233 Tutors  Schedule an appointment 541-917-4697 ...
p53-associated 3 exonuclease activity in nuclear and cytoplasmic
p53-associated 3 exonuclease activity in nuclear and cytoplasmic

... exonuclease activity of p53 must not be restricted to its noninduced state, but might also be exerted by a subclass of p53 after DNA damage when the protein is able to display its full range of possible biochemical activities (Albrechtsen et al., 1999). p53 was found in the nucleus, the cytoplasm or ...
Assembly of RecA-like Recombinases
Assembly of RecA-like Recombinases

... In addition to the Rad51 paralogues, the breast cancer susceptibility genes BRCA1 (85) and BRCA2 (86) have been shown to be important for formation of Rad51 foci after DNA damage. The BRCA proteins had been shown previously to associate with Rad51 (75–81) and to colocalize with damage-induced Rad51 ...
Lesson Overview - Diman Regional
Lesson Overview - Diman Regional

... membrane of their target cells. Nonsteroid hormones bind to receptors in a target cell and cause the release of secondary messengers that affect cell activities. ...
Caspases - Essays in Biochemistry
Caspases - Essays in Biochemistry

... domains) found in pro-caspases 1, 2, 4, 5 and 9, which are important for the activation of these enzymes [10]. Activation of caspases containing long prodomains first requires oligomerization via DED or CARD domains. Following the recruitment of a single type of caspase pro-enzyme to a common oligom ...
Getting to the root of plant biology: impact of the Arabidopsis
Getting to the root of plant biology: impact of the Arabidopsis

... nagging doubt that responses specific to individual cell types or tissues would be missed, as they were diluted out in the mass of the whole plant or organ. To address this concern, new approaches were needed to allow expression analysis of only one cell type or developmental stage. The three approa ...
Morphine: CYP2D6 Modulation Human White Blood Cells Synthesize
Morphine: CYP2D6 Modulation Human White Blood Cells Synthesize

... cells; 3, PMN incubated with 10⫺6 M L-DOPA; 4, incubated with 50% L-DOPA-exposed cells and 50% IL-1␤-exposed cells for 1 h, exhibiting a lower level of activation compared to treatment 2; 5, PMN incubated with 50% L-DOPA-exposed cells and 50% IL-1␤-exposed cells for 1 h; 5 min before being exposed t ...
Sphingolipids Containing Very-Long
Sphingolipids Containing Very-Long

... 2010). In all these mutants, the level of VLCFA in sphingolipids is reduced, and this reduction is coupled with important morphological changes in the plant. In the cer10 mutant, which is deficient in elongation-specific enoyl reductase, endosomal compartments were shown to accumulate, indicating ab ...
Hox genes, neural crest cells and branchial arch patterning Paul A
Hox genes, neural crest cells and branchial arch patterning Paul A

... The cranial neural crest is a pluripotent, mesenchymal population that plays a critical role in construction of the vertebrate head. Arising at the junction between the neural plate and surface ectoderm, cranial neural crest cells form nerve, ganglia, cartilage, bone and connective tissue. Many cran ...
Biology Overview and Supportive Laboratory Activities
Biology Overview and Supportive Laboratory Activities

... them (indicated as “also assesses” on the assessment documents). As shown in Table 1, the keystone standards have supporting standards beneath them. For example, N.1.1 also assesses N.1.4, N.1.6 and L.14.4. These are the supporting standards for N.1.1. Table 1 shows the connection between the keysto ...
Word document
Word document

... chromatinized template DNA but not when using naked DNA lacking histones (Kraus and Kadonaga, 1998). In addition, genes integrated into chromosomes have been shown to be regulated differently from genes located in transiently transfected plasmid DNA (Fryer and Archer, 1998), often revealing surprisi ...
Developmental Control of Telomere Lengths and Telomerase
Developmental Control of Telomere Lengths and Telomerase

... ibp.cz; fax 420-5-41-24-05-00. ...
Skeletal Muscles
Skeletal Muscles

... Each skeletal muscle fiber is an elongated cell invested by a delicate membrane that, by electron microscopy, can be resolved into the plasmalemma and an adherent outer layer of glycoproteins and glycosaminoglycans. The plasmalemma of a muscle cell is referred to as the sarcolemma but is no differen ...
A Definitive, Rapid Alternative to the Gram Stain Assay
A Definitive, Rapid Alternative to the Gram Stain Assay

... The Gram stain assay differentiates bacteria into two groups. Gram positive organisms have high levels of peptidoglycan in their cell walls, which retain the primary crystal violet stain and appear purple. The Gram negative cell wall contains small levels of peptidoglycan but also contains an outer ...
Gene Section KSR1 (kinase suppressor of ras 1)
Gene Section KSR1 (kinase suppressor of ras 1)

... Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrolysis in the putative kinase domain. This lysine is ...
The tubby-like proteins - Journal of Cell Science
The tubby-like proteins - Journal of Cell Science

... electron-density map obtained was interpreted as a unique protein structure, a 12-stranded β-barrel conformation filled with a central hydrophobic core that traverses the entire barrel. Two prominent features of the folded protein are a large groove of positively charged residues and a smaller regio ...
PDF
PDF

... few of these genes are the same as the genes expressed during sporulation in S. cerevisiae (Mata et al., 2002). This observation suggests that in addition to meiotic processes conserved across eukaryotes, sporulation, including meiosis, involves processes that are more evolutionarily divergent and r ...
PDF
PDF

... gradually coats the Xp during pre-implantation development (Huynh and Lee, 2003; Okamoto et al., 2004). By the early blastocyst stage, prior to specification of extra-embryonic and embryonic lineages, Xist accumulation on the Xp (Xp-Xist coating) is evident in all cells of the embryo (Sheardown et a ...
Mitogen-Activated Protein Kinases and Mitogen
Mitogen-Activated Protein Kinases and Mitogen

... al., 2004). These findings imply that MAPK signaling may play an important role in the mechano- and signal transduction of extracellular stimuli to intracellular biological responses that control muscle fiber viability. However, the reports regarding the activity of the MAPKs in the pathogenesis of ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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