PDF - Journal of Neuroscience
... (ARID1a), BAF250b (ARID1b), BAF57, and BAF47, were linked to Coffin-Siris Syndrome (CSS), a sporadic intellectual disability syndrome (Santen et al., 2012; Tsurusaki et al., 2012). Eightyseven percent of CSS patients in these studies had a mutation in a BAF subunit, underscoring how mutations in gen ...
... (ARID1a), BAF250b (ARID1b), BAF57, and BAF47, were linked to Coffin-Siris Syndrome (CSS), a sporadic intellectual disability syndrome (Santen et al., 2012; Tsurusaki et al., 2012). Eightyseven percent of CSS patients in these studies had a mutation in a BAF subunit, underscoring how mutations in gen ...
Cytoplasmic streaming in plants
... bound to actin per ATP hydrolysis cycle) observed for myosin could not explain the high-velocity sliding movement seen in characean cells (up to 100 mm/s, depending on the measuring temperature). This velocity is almost ten times higher that that of cytoplasmic streaming in cells of higher plants an ...
... bound to actin per ATP hydrolysis cycle) observed for myosin could not explain the high-velocity sliding movement seen in characean cells (up to 100 mm/s, depending on the measuring temperature). This velocity is almost ten times higher that that of cytoplasmic streaming in cells of higher plants an ...
Transcriptional and epigenetic control of gene expression in embryo
... The eukaryotic cell must organize the DNA into a more compact form in order to fit the large amount of DNA into the tiny nucleus. This is achieved by wrapping 147bp of DNA in two super-helical turns around an octamer of histones (two of each H2A, H2B, H3 and H4) forming the basic unit of chromatin, ...
... The eukaryotic cell must organize the DNA into a more compact form in order to fit the large amount of DNA into the tiny nucleus. This is achieved by wrapping 147bp of DNA in two super-helical turns around an octamer of histones (two of each H2A, H2B, H3 and H4) forming the basic unit of chromatin, ...
Budding Yeast for Budding Geneticists: A Primer on the
... by their owners. In the late 1800s, the Carlsberg Laboratory introduced the brewing industry to the process of science, and Emil Christian Hansen discovered how to purify yeast from mixed starter cultures (Greig and Leu 2009). In the past 60 years, a rather short time evolutionarily speaking, the st ...
... by their owners. In the late 1800s, the Carlsberg Laboratory introduced the brewing industry to the process of science, and Emil Christian Hansen discovered how to purify yeast from mixed starter cultures (Greig and Leu 2009). In the past 60 years, a rather short time evolutionarily speaking, the st ...
Cell-penetrating peptides and antimicrobial peptides: how different
... deliver their cargoes in the cytosol is far from being totally understood. Although some CPPs are able to translocate by an endocytic pathway, the escape from endosomes demands a physically driven mechanism. The affinity of each CPP for lipid bilayers may be the key factor for their main mechanism o ...
... deliver their cargoes in the cytosol is far from being totally understood. Although some CPPs are able to translocate by an endocytic pathway, the escape from endosomes demands a physically driven mechanism. The affinity of each CPP for lipid bilayers may be the key factor for their main mechanism o ...
MEMBRANE PROTEINS SYNTHESIZED BY
... ABSTRACT Intact rabbit reticulocyte cells synthesize two predominant species of polypeptides which are components of the cell plasma membrane. Previous work (Lodish, H. F. 1973. Proc. Natl. Acad. Sci. U. S. A. 70:1526-1530.) showed that these proteins were synthesized by polyribosomes not attached t ...
... ABSTRACT Intact rabbit reticulocyte cells synthesize two predominant species of polypeptides which are components of the cell plasma membrane. Previous work (Lodish, H. F. 1973. Proc. Natl. Acad. Sci. U. S. A. 70:1526-1530.) showed that these proteins were synthesized by polyribosomes not attached t ...
PDF
... however, are not present in every adult tissue. Two basic actin isoforms, which probably correspond to the vertebrate /? and y cytoskeletal actins, are present in body wall muscle, branchial sac, gut and gonads. An acidic actin isoform, which probably corresponds to a vertebrate a muscle actin, is p ...
... however, are not present in every adult tissue. Two basic actin isoforms, which probably correspond to the vertebrate /? and y cytoskeletal actins, are present in body wall muscle, branchial sac, gut and gonads. An acidic actin isoform, which probably corresponds to a vertebrate a muscle actin, is p ...
Aubé et al., J Immunol, 2014
... 27). The activity of IFN-g–producing Th1 lymphocytes is suppressed by neutrophils in the CNS of EAE mice, and neutrophilderived myeloperoxidase was shown to inhibit dendritic cell activation, therefore dampening T cell–driven inflammation (28, 29). Aside from their capacity to secrete a vast array o ...
... 27). The activity of IFN-g–producing Th1 lymphocytes is suppressed by neutrophils in the CNS of EAE mice, and neutrophilderived myeloperoxidase was shown to inhibit dendritic cell activation, therefore dampening T cell–driven inflammation (28, 29). Aside from their capacity to secrete a vast array o ...
A Multifunctional Cell Surface Developmental Stage
... In outline, the immunoprecipitation and fractionation of the DSS-8 antigen entails the biotinylation of all proteins, solubilization in 1% CHAPS, incubation with immobilized mAb, extensive washing of the antigen-mAb complex, elution of the antigen, fractionation by SDS-PAGE, transfer to nitrocellul ...
... In outline, the immunoprecipitation and fractionation of the DSS-8 antigen entails the biotinylation of all proteins, solubilization in 1% CHAPS, incubation with immobilized mAb, extensive washing of the antigen-mAb complex, elution of the antigen, fractionation by SDS-PAGE, transfer to nitrocellul ...
Diapositiva 1 - the Future Health Summit
... pcg (picogram)= 10 -12 = 0.000000000001 fg (fentogram)= 10 -15 = 0.000000000000001 © Dipartimento Scientifico Guna S.p.a. ...
... pcg (picogram)= 10 -12 = 0.000000000001 fg (fentogram)= 10 -15 = 0.000000000000001 © Dipartimento Scientifico Guna S.p.a. ...
Two overlapping reading frames in a single exon encode interacting
... Fig. 1. Existence of two overlapping open reading frames in the XL-exon. (A) The XL-exon of the rat XLas/Gas gene (DDBJ/EMBL/GenBank accession No. AF093569) contains two ORFs. ORF1 (1) encodes the XL-domain of XLas, ORF2 (2) the ALEX protein. The third reading frame (3) does not encode a protein due ...
... Fig. 1. Existence of two overlapping open reading frames in the XL-exon. (A) The XL-exon of the rat XLas/Gas gene (DDBJ/EMBL/GenBank accession No. AF093569) contains two ORFs. ORF1 (1) encodes the XL-domain of XLas, ORF2 (2) the ALEX protein. The third reading frame (3) does not encode a protein due ...
Muscle Injury and the Role of Myosatellite Cells in Muscle Healing
... muscle fibers have been torn but the tear does not go all the way through the thickness of the muscle. A third-degree tear is the most painful and serious type of tear. In a third-degree muscle tear or rupture, the muscle is torn all the way across its width and it is impossible to contract it. The ...
... muscle fibers have been torn but the tear does not go all the way through the thickness of the muscle. A third-degree tear is the most painful and serious type of tear. In a third-degree muscle tear or rupture, the muscle is torn all the way across its width and it is impossible to contract it. The ...
VASCULAR TISSUE DIFFERENTIATION AND
... procambial cells and promote their division, which continuously provides precursor cells for differentiation of xylem and phloem. It has long been proposed that auxin, which is polarly transported from shoot apical meristem and young leaves, induces formation of procambial cells. Early physiological ...
... procambial cells and promote their division, which continuously provides precursor cells for differentiation of xylem and phloem. It has long been proposed that auxin, which is polarly transported from shoot apical meristem and young leaves, induces formation of procambial cells. Early physiological ...
PDF - Potter Lab
... clones in the eye and wing discs and found that mosaic discs were two to three times larger than wild-type controls (Figures 2A and 2B, data not shown). Phalloidin staining revealed that the Tsc1 mutant cells anterior and posterior to the morphogenetic furrow of the third instar eye disc were alread ...
... clones in the eye and wing discs and found that mosaic discs were two to three times larger than wild-type controls (Figures 2A and 2B, data not shown). Phalloidin staining revealed that the Tsc1 mutant cells anterior and posterior to the morphogenetic furrow of the third instar eye disc were alread ...
1 A Single-Molecule Hershey-Chase Experiment
... experiments [15,16], as the bulk data should only be compared to the fastest observed single-molecule ejections, since Southern blot analysis would pick out the earliest infections, but not the entire distribution of infection times. A number of different hypotheses have been formulated for the actu ...
... experiments [15,16], as the bulk data should only be compared to the fastest observed single-molecule ejections, since Southern blot analysis would pick out the earliest infections, but not the entire distribution of infection times. A number of different hypotheses have been formulated for the actu ...
Update on Reactive Oxygen - Biology Department | UNC Chapel Hill
... overlap between different Rboh proteins. For example, in Arabidopsis, various phenotypes of the individual atrbohD and atrbohF mutants are accentuated in the double mutant atrbohD atrbohF (Torres et al., 2002; Kwak et al., 2003). Thus, while the Rboh proteins are required for ROS production followin ...
... overlap between different Rboh proteins. For example, in Arabidopsis, various phenotypes of the individual atrbohD and atrbohF mutants are accentuated in the double mutant atrbohD atrbohF (Torres et al., 2002; Kwak et al., 2003). Thus, while the Rboh proteins are required for ROS production followin ...
Human Fatty Acid Transport Protein 2a/Very Long Chain Acyl
... fatty acids into complex lipid pools (26). Overexpression studies examining the function of Acsl1, for example, demonstrated that this enzyme provides an important role in triglyceride synthesis in cardiac myocytes, hepatocytes, and NIH 3T3 cells (27– 29). Triacsin C (an Acsl inhibitor) treatment re ...
... fatty acids into complex lipid pools (26). Overexpression studies examining the function of Acsl1, for example, demonstrated that this enzyme provides an important role in triglyceride synthesis in cardiac myocytes, hepatocytes, and NIH 3T3 cells (27– 29). Triacsin C (an Acsl inhibitor) treatment re ...
ATP as a Signaling Molecule: the Exocrine Focus
... responses, platelet aggregation, endothelial release of vasorelaxants, immune defense, cell volume regulation, cell proliferation and mitogenesis, apoptosis, and epithelial ion and water transport, just to name a few (Fig. 1). Whether and how ATP will act on the cell depends on several interrelated ...
... responses, platelet aggregation, endothelial release of vasorelaxants, immune defense, cell volume regulation, cell proliferation and mitogenesis, apoptosis, and epithelial ion and water transport, just to name a few (Fig. 1). Whether and how ATP will act on the cell depends on several interrelated ...
Intermetallic Catalysts: An Efficient Approach for the Reduction in the
... been utilized for both anode and cathode catalysts which are immobilized in the form of nanoparticles on supporting materials for instance, carbon. Although PGM showed an outstanding catalytic behavior towards fuel cell applications, however, there would be an essential need to investigate the catal ...
... been utilized for both anode and cathode catalysts which are immobilized in the form of nanoparticles on supporting materials for instance, carbon. Although PGM showed an outstanding catalytic behavior towards fuel cell applications, however, there would be an essential need to investigate the catal ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... closely associated fibrillar centers (FC). The early processing of rRNAs occurs in the DFC and ribosomal subunits are assembled in the granular component (GC; Shaw and Jordan, 1995; Scheer and Hock, 1999; Carmo-Fonseca et al., 2000). In plant nucleoli, the active rDNA transcription units are well di ...
... closely associated fibrillar centers (FC). The early processing of rRNAs occurs in the DFC and ribosomal subunits are assembled in the granular component (GC; Shaw and Jordan, 1995; Scheer and Hock, 1999; Carmo-Fonseca et al., 2000). In plant nucleoli, the active rDNA transcription units are well di ...
Insights into the Role of Specific Lipids in the
... The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton ...
... The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton ...
Cholesterol and bile acids regulate xenosensor signaling in
... Since LXR and CXR bind to the same DR-4 element, electromobility-shift assays were used to elucidate if LXR and CXR directly compete for binding to this PBRU. To clearly discriminate between complexes containing CXR or LXR, a HA-tag was N-terminally attached to CXR and an anti-HA monoclonal antibody ...
... Since LXR and CXR bind to the same DR-4 element, electromobility-shift assays were used to elucidate if LXR and CXR directly compete for binding to this PBRU. To clearly discriminate between complexes containing CXR or LXR, a HA-tag was N-terminally attached to CXR and an anti-HA monoclonal antibody ...
Adaptative Response of Vitis Root to Anoxia
... ATP by anaerobic glycolysis, a process that yields 2–3 mol ATP mol–1 glucose. This should be compared with a yield of approximately 24–36 mol ATP mol–1 glucose during aerobic oxidative phosphorylation (Gibbs and Greenway 2003). Since during anoxia every molecule of glucose yields less than onetenth ...
... ATP by anaerobic glycolysis, a process that yields 2–3 mol ATP mol–1 glucose. This should be compared with a yield of approximately 24–36 mol ATP mol–1 glucose during aerobic oxidative phosphorylation (Gibbs and Greenway 2003). Since during anoxia every molecule of glucose yields less than onetenth ...