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... Gene knock-out studies in mice are starting to reveal the physiological significance of sphingolipids in mammals. First of all, in addition to phosphosphingolipids being indispensable for cell proliferation (see sect. IIA), glycosphingolipid production is essential for development and differentiatio ...
... Gene knock-out studies in mice are starting to reveal the physiological significance of sphingolipids in mammals. First of all, in addition to phosphosphingolipids being indispensable for cell proliferation (see sect. IIA), glycosphingolipid production is essential for development and differentiatio ...
Solid Tumour Section Testis: Spermatocytic seminoma Atlas of Genetics and Cytogenetics
... mutations in FGFR3 or HRAS in about 25% of tumour specimens (Goriely et al., 2009). This finding suggested that random mutational events may occur in spermatogonia and accumulate with age, leading to a selective proliferation of cells with mutations that give them growth or survival advantage and ev ...
... mutations in FGFR3 or HRAS in about 25% of tumour specimens (Goriely et al., 2009). This finding suggested that random mutational events may occur in spermatogonia and accumulate with age, leading to a selective proliferation of cells with mutations that give them growth or survival advantage and ev ...
Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis
... Surprisingly, neither zygotic stat3stl27/stl27 nor stat3stl28/stl28 mutant embryos showed overt gastrulation defects described in the previous morpholino studies [12], and displayed normal morphology and notochord formation until 15 dpf (Fig 2A and 2D). During later larval stages, stat3stl28/stl28 ( ...
... Surprisingly, neither zygotic stat3stl27/stl27 nor stat3stl28/stl28 mutant embryos showed overt gastrulation defects described in the previous morpholino studies [12], and displayed normal morphology and notochord formation until 15 dpf (Fig 2A and 2D). During later larval stages, stat3stl28/stl28 ( ...
Implications of streamlining theory for microbial ecology
... smaller microbial genomes than those of streamlined, free-living cells. In many cases, these associations are between bacteria and eukaryotes, although there are examples of bacterial and archaeal symbionts with other bacteria or archaea (Baker et al., 2010). Genome reduction in SPC organisms occurs ...
... smaller microbial genomes than those of streamlined, free-living cells. In many cases, these associations are between bacteria and eukaryotes, although there are examples of bacterial and archaeal symbionts with other bacteria or archaea (Baker et al., 2010). Genome reduction in SPC organisms occurs ...
paramecium notes 14
... membrane called the pellicle. The pellicle surrounds the cell membrane. Color the pellicle blue. The cell membrane is a thin layer just inside the pellicle. Color the cell membrane light blue. There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which contr ...
... membrane called the pellicle. The pellicle surrounds the cell membrane. Color the pellicle blue. The cell membrane is a thin layer just inside the pellicle. Color the cell membrane light blue. There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which contr ...
Shared versus Specialized Glycinergic Spinal Interneurons in Axial
... MgCl2, 10 mM EGTA, 10 mM HEPES buffer, 4 mM Na2ATP, 0.1% Sulforhodamine B (Sigma), and adjusted to a pH of 7.3 with potassium hydroxide. As in a previous study (Bhatt et al., 2007), the calculated junction potential using this recording solution was 16 mV, and we did not correct for it in the figure ...
... MgCl2, 10 mM EGTA, 10 mM HEPES buffer, 4 mM Na2ATP, 0.1% Sulforhodamine B (Sigma), and adjusted to a pH of 7.3 with potassium hydroxide. As in a previous study (Bhatt et al., 2007), the calculated junction potential using this recording solution was 16 mV, and we did not correct for it in the figure ...
multiple novel factors regulate the initiation of dna
... thousands of sites along chromosomes termed origins of replication. Although many of the essential protein components catalyzing events at these sites are known and are conserved throughout eukaryotes, the likelihood or efficiency of initiation of DNA synthesis at any given genomic site is expected ...
... thousands of sites along chromosomes termed origins of replication. Although many of the essential protein components catalyzing events at these sites are known and are conserved throughout eukaryotes, the likelihood or efficiency of initiation of DNA synthesis at any given genomic site is expected ...
Cell Wall, Cytoskeleton, and Cell Expansion in Higher Plants
... Xyloglucan (XyG) is the most abundant hemicellulose in dicot primary walls. XyG is thought to form hydrogen bonds along the lengths of cellulose microfibrils and to play a large role in forming the crosslinks between cellulose microfibrils. Historically, there have been two proposed mechanisms by wh ...
... Xyloglucan (XyG) is the most abundant hemicellulose in dicot primary walls. XyG is thought to form hydrogen bonds along the lengths of cellulose microfibrils and to play a large role in forming the crosslinks between cellulose microfibrils. Historically, there have been two proposed mechanisms by wh ...
paramecium notes 13 highlighted
... membrane called the pellicle. The pellicle surrounds the cell membrane. Color the pellicle blue. The cell membrane is a thin layer just inside the pellicle. Color the cell membrane light blue. There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which contr ...
... membrane called the pellicle. The pellicle surrounds the cell membrane. Color the pellicle blue. The cell membrane is a thin layer just inside the pellicle. Color the cell membrane light blue. There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which contr ...
Ribosylation of bovine serum albumin induces ROS accumulation
... AGEs has become an important area of biomedical research. Although much work has been conducted on glycation of proteins with glucose, few research groups have attempted to study glycation by ribose and resulting effects on cell structure and function (Chanshuai et al. 2011). In recent years, ribosy ...
... AGEs has become an important area of biomedical research. Although much work has been conducted on glycation of proteins with glucose, few research groups have attempted to study glycation by ribose and resulting effects on cell structure and function (Chanshuai et al. 2011). In recent years, ribosy ...
Evidence for Multiple Calcium Response
... citralva, citronellal, eugenol, geraniol, hedione, menthone and phenethylalcohol, while Mix B [IP3-producing odors in rat biochemical assays (Breer and Boekhoff, 1991)] contained ethyl vanillin, isovaleric acid, lilial, lyral, phenylethylamine and triethylamine. In some experiments, subsets of these ...
... citralva, citronellal, eugenol, geraniol, hedione, menthone and phenethylalcohol, while Mix B [IP3-producing odors in rat biochemical assays (Breer and Boekhoff, 1991)] contained ethyl vanillin, isovaleric acid, lilial, lyral, phenylethylamine and triethylamine. In some experiments, subsets of these ...
Inhibition Systems Mechanisms and Biological Roles of
... CdiA-CT toxin binds tightly to a cysteine biosynthetic enzyme (CysK) in the cytoplasm of target cells. The unanticipated complexity in the UPEC CDI pathway raises the possibility that these systems perform other functions in addition to growth inhibition. Finally, we propose that the phenomenon of C ...
... CdiA-CT toxin binds tightly to a cysteine biosynthetic enzyme (CysK) in the cytoplasm of target cells. The unanticipated complexity in the UPEC CDI pathway raises the possibility that these systems perform other functions in addition to growth inhibition. Finally, we propose that the phenomenon of C ...
Chemical Effectors of Plant Endocytosis and Endomembrane
... Chap. 1 by Šamajová et al. in this volume). In recent years, the introduction of genetically encoded fluorophores, such as GFP and its mutated isoforms (Chalfie et al. 1994), have further facilitated the observation of subcellular membrane structures. GFP labeling of marker proteins has provided det ...
... Chap. 1 by Šamajová et al. in this volume). In recent years, the introduction of genetically encoded fluorophores, such as GFP and its mutated isoforms (Chalfie et al. 1994), have further facilitated the observation of subcellular membrane structures. GFP labeling of marker proteins has provided det ...
The Molecular Basis of the Evolution and Diversity of Stomatal
... into pavement cells. The MMC divides asymmetrically to form a meristemoid (M) and SLGC and may reiterate similar divisions several times. MUTE controls the cell-state transition from M to GMC, and FAMA is required for correct division of the GMC into GCs forming a functional stoma. It is proposed th ...
... into pavement cells. The MMC divides asymmetrically to form a meristemoid (M) and SLGC and may reiterate similar divisions several times. MUTE controls the cell-state transition from M to GMC, and FAMA is required for correct division of the GMC into GCs forming a functional stoma. It is proposed th ...
Western et al., 2001 - UBC Blogs
... involves a regulated series of cytological events including growth, cytoplasmic rearrangement, mucilage synthesis, and secondary cell wall production. We have tested the potential of Arabidopsis seed coat epidermal cells as a model system for the genetic analysis of these processes. A screen for mut ...
... involves a regulated series of cytological events including growth, cytoplasmic rearrangement, mucilage synthesis, and secondary cell wall production. We have tested the potential of Arabidopsis seed coat epidermal cells as a model system for the genetic analysis of these processes. A screen for mut ...
bacterial isolates of marine coast as commercial producer of protease
... bacilli while SD3 and SV1 were bacilli in chain. The Gram nature was also reconfirmed by the Real time PCR based detection method. Except SD1 all other isolates were gram positive while only SD1 showed presence of capsule. One of the important facts observed was the presence of endospores in all the ...
... bacilli while SD3 and SV1 were bacilli in chain. The Gram nature was also reconfirmed by the Real time PCR based detection method. Except SD1 all other isolates were gram positive while only SD1 showed presence of capsule. One of the important facts observed was the presence of endospores in all the ...
Protein secretion and surface display in Gram
... YSIRK/GS signal peptides are eventually distributed over the entire bacterial surface, whereas those that are immobilized following secretion via canonical signal peptides reside only at the cell poles [63,64]. Recent work identified genes for membrane proteins with abortive-infectivity domains, des ...
... YSIRK/GS signal peptides are eventually distributed over the entire bacterial surface, whereas those that are immobilized following secretion via canonical signal peptides reside only at the cell poles [63,64]. Recent work identified genes for membrane proteins with abortive-infectivity domains, des ...
Supplementary Information
... resuspended in phosphate buffered saline (PBS) and then captured onto MSD 96well high bind plates at 30,000 cells per well by incubating at 37⁰C for 1.5 hours. The plates were blocked with 15% fetal bovine serum (FBS) (or heat-inactivated FBS) for 30 min at room temperature with gentle agitation. Th ...
... resuspended in phosphate buffered saline (PBS) and then captured onto MSD 96well high bind plates at 30,000 cells per well by incubating at 37⁰C for 1.5 hours. The plates were blocked with 15% fetal bovine serum (FBS) (or heat-inactivated FBS) for 30 min at room temperature with gentle agitation. Th ...
CDP1, a novel component of chloroplast division site
... overexpressing AtCDP1 were heterogeneous and the division site was misplaced (Figure 1A, 1B). Although two gene structures of AtCDP1 were present in the NCBI database (Figure 2M), only one of them was obtained by screening the expression library (shown with a black asterisk in Figure 2M). Moreover, ...
... overexpressing AtCDP1 were heterogeneous and the division site was misplaced (Figure 1A, 1B). Although two gene structures of AtCDP1 were present in the NCBI database (Figure 2M), only one of them was obtained by screening the expression library (shown with a black asterisk in Figure 2M). Moreover, ...
Membrane trafficking and polar growth in root hairs and pollen tubes
... form in which the cell elongates unidirectionally and only at the very tip. This extreme form of polarized growth, or tip-growth, sustains prodigious rates of elongation: Root hairs grow at rates of 10–40 nm sÿ1 (Galway et al., 1997; Wymer et al., 1997), comparable to that of animal neuron growth co ...
... form in which the cell elongates unidirectionally and only at the very tip. This extreme form of polarized growth, or tip-growth, sustains prodigious rates of elongation: Root hairs grow at rates of 10–40 nm sÿ1 (Galway et al., 1997; Wymer et al., 1997), comparable to that of animal neuron growth co ...
Phloem Structure and Function
... Sieve element structure and association with companion cells or Strasburger cells The assimilate-conducting sieve elements of the phloem are the most highly specialized cell type in plants. During development, flow resistance in these cells is reduced by changes in the protoplast. In addition, a func ...
... Sieve element structure and association with companion cells or Strasburger cells The assimilate-conducting sieve elements of the phloem are the most highly specialized cell type in plants. During development, flow resistance in these cells is reduced by changes in the protoplast. In addition, a func ...
In tobacco leaf epidermal cells, the integrity of protein export from
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
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... (see supplementary material Fig. S1K,L) and the motoneuronspecific D42-Gal4 (Parkes et al., 1998), when used to target Gαo through expression of RNAi or Ptx, also led to a substantial decrease in bouton numbers (Fig. 2B). The Wg-secreting type Ib boutons (Packard et al., 2002) appeared more severely ...
... (see supplementary material Fig. S1K,L) and the motoneuronspecific D42-Gal4 (Parkes et al., 1998), when used to target Gαo through expression of RNAi or Ptx, also led to a substantial decrease in bouton numbers (Fig. 2B). The Wg-secreting type Ib boutons (Packard et al., 2002) appeared more severely ...
Mechanisms of liver allograft rejection in man
... T-lymphocytes with their target cells. As a general rule class I antigens serve as the primary targets for cytotoxic T-lymphocytes and class I1 antigens as the primary targets for helper T-lymphocytes [30-321. The expression of MHC antigens varies between different tissues. In human liver HLA class ...
... T-lymphocytes with their target cells. As a general rule class I antigens serve as the primary targets for cytotoxic T-lymphocytes and class I1 antigens as the primary targets for helper T-lymphocytes [30-321. The expression of MHC antigens varies between different tissues. In human liver HLA class ...
