Shift in GATA3 functions, and GATA3 mutations, control progression
... percent were cell-specific (Figure 2A), pointing to altered targets recognition by GATA3 following tumorigenesis. In both samples, 46% of GATA3 binding sites were inside or within 10 kbp of coding sequences (Figure 2B). Of these, among GO annotated genes [36], large groups are associated with cellul ...
... percent were cell-specific (Figure 2A), pointing to altered targets recognition by GATA3 following tumorigenesis. In both samples, 46% of GATA3 binding sites were inside or within 10 kbp of coding sequences (Figure 2B). Of these, among GO annotated genes [36], large groups are associated with cellul ...
Glucose and forskolin regulate IAPP gene expression through
... reaching 80% confluence and were plated into 12-well plates (300,000 cells/well) for Northern blot analysis or into 100-mm dishes (1.5 ⫻ 106/dish) for transfection and electrophoretic mobility shift assay (EMSA). Cells of passages 65 through 85 were used in this study. For studying the effects of gl ...
... reaching 80% confluence and were plated into 12-well plates (300,000 cells/well) for Northern blot analysis or into 100-mm dishes (1.5 ⫻ 106/dish) for transfection and electrophoretic mobility shift assay (EMSA). Cells of passages 65 through 85 were used in this study. For studying the effects of gl ...
Molecular Microbiology
... both are novel cyclic lipodepsipeptides with a hexanoic lipid chain connected to the N-terminus of a 12amino-acid peptide moiety, in which the C-terminal carboxylic acid group forms an ester with the hydroxyl side-chain of Ser9. The difference between the two structures is located in the second amin ...
... both are novel cyclic lipodepsipeptides with a hexanoic lipid chain connected to the N-terminus of a 12amino-acid peptide moiety, in which the C-terminal carboxylic acid group forms an ester with the hydroxyl side-chain of Ser9. The difference between the two structures is located in the second amin ...
FKBP12-rapamycin-associated protein associates
... Fig. 3. Analysis of FRAP immune-complex and localization of FRAP by subcellular fractionation studies. (A) Identification of components of PDH complex in FRAP immune-complexes. Jurkat cells were lysed as described in Materials and Methods and the cell lysate was used to isolate FRAP immune-complexes ...
... Fig. 3. Analysis of FRAP immune-complex and localization of FRAP by subcellular fractionation studies. (A) Identification of components of PDH complex in FRAP immune-complexes. Jurkat cells were lysed as described in Materials and Methods and the cell lysate was used to isolate FRAP immune-complexes ...
Promoting central nervous system regeneration: Lessons from
... lifespan in the normal OE is less clear. A number of original studies have put forward an average turnover rate of approximately 4 weeks (e.g. Moulton, 1974; Graziadei & Monti-Graziadei, 1979). However, others have challenged this view and suggested this turnover might be much slower with some recep ...
... lifespan in the normal OE is less clear. A number of original studies have put forward an average turnover rate of approximately 4 weeks (e.g. Moulton, 1974; Graziadei & Monti-Graziadei, 1979). However, others have challenged this view and suggested this turnover might be much slower with some recep ...
AHEART December 46/6 - AJP
... The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. H2442 ...
... The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. H2442 ...
REVIEWS - Geobacter Project
... alleviates the need for the complex and highly regulated distribution systems that are required for hydrogen and methanol. Therefore, microbial fuel cells might be particularly attractive power sources in remote locations and regions of developing countries that are not served by well-developed, cen ...
... alleviates the need for the complex and highly regulated distribution systems that are required for hydrogen and methanol. Therefore, microbial fuel cells might be particularly attractive power sources in remote locations and regions of developing countries that are not served by well-developed, cen ...
manipulation of respiratory burst of neutrophils using c1
... Figure 4-20 Effects of 0.2% Triton X-100 and 0.1mM EDTA on activity of extracted PMN SOD or bovine erythrocytes SOD...................................................................................... 79 Figure 4-21 Effects of Triton X-100 on extracted SOD from WBC and EDTA. ................... 80 ...
... Figure 4-20 Effects of 0.2% Triton X-100 and 0.1mM EDTA on activity of extracted PMN SOD or bovine erythrocytes SOD...................................................................................... 79 Figure 4-21 Effects of Triton X-100 on extracted SOD from WBC and EDTA. ................... 80 ...
Failsafe Mechanisms Coordinate Cell Division and the Initiation of
... This view of the cell cycle is complicated when cells are grown in rich media. Bacteria can have doubling rates that are ~20-‐25 minutes, which is much faster than the time it takes for chromosom ...
... This view of the cell cycle is complicated when cells are grown in rich media. Bacteria can have doubling rates that are ~20-‐25 minutes, which is much faster than the time it takes for chromosom ...
The unfolded protein response: controlling cell fate
... of eukaryotic translation initiator factor 2α (eIF2α)), activating mRNA decay by regulated inositol-requiring protein 1 (IRE1)dependent decay (RIDD), and activating autophagy through the IRE1α–JUN N‑terminal kinase (JNK) pathway. In a second wave of events, the UPR transcription factors activating t ...
... of eukaryotic translation initiator factor 2α (eIF2α)), activating mRNA decay by regulated inositol-requiring protein 1 (IRE1)dependent decay (RIDD), and activating autophagy through the IRE1α–JUN N‑terminal kinase (JNK) pathway. In a second wave of events, the UPR transcription factors activating t ...
WAVE/Scars in platelets
... glutathione Sepharose, which had been immobilized with GST or GST-SH3 of abl and then incubated with platelet lysates, were examined for the presence of WAVE1 (A), WAVE2 (B), or WAVE3 (C), as indicated by SDS-PAGE electrophoresis followed by immunoblotting. For WAVE3, 2 polyclonal antibodies were us ...
... glutathione Sepharose, which had been immobilized with GST or GST-SH3 of abl and then incubated with platelet lysates, were examined for the presence of WAVE1 (A), WAVE2 (B), or WAVE3 (C), as indicated by SDS-PAGE electrophoresis followed by immunoblotting. For WAVE3, 2 polyclonal antibodies were us ...
An Empirical Study of End-User Behaviour in Spreadsheet Error
... code inspection phase of any kind. In an attempt to determine what the average error correction yield might have been if individuals had been placed in groups of three, all combinations of three students were created and the number of separate errors corrected identified. The average performance acr ...
... code inspection phase of any kind. In an attempt to determine what the average error correction yield might have been if individuals had been placed in groups of three, all combinations of three students were created and the number of separate errors corrected identified. The average performance acr ...
VIRUS-LIKE PARTICLES ELICIT ENHANCED IMMUNITY TO
... I would first like to thank my advisor, Dr. Allen Harmsen for his years of guidance, support, insight, and patience. His mentorship and wisdom have been of immense value to me. I would also like to thank my committee for their leadership in directing my project, as well as my career. The Harmsen lab ...
... I would first like to thank my advisor, Dr. Allen Harmsen for his years of guidance, support, insight, and patience. His mentorship and wisdom have been of immense value to me. I would also like to thank my committee for their leadership in directing my project, as well as my career. The Harmsen lab ...
T Cells FOXP3 Target Genes in Natural Regulatory Genome
... this, we have performed a comprehensive genome-wide analysis for human FOXP3 target genes from cord blood Tregs using chromatin immunoprecipitation array profiling and expression profiling. We have identified 5579 human FOXP3 target genes and derived a core Treg gene signature conserved across speci ...
... this, we have performed a comprehensive genome-wide analysis for human FOXP3 target genes from cord blood Tregs using chromatin immunoprecipitation array profiling and expression profiling. We have identified 5579 human FOXP3 target genes and derived a core Treg gene signature conserved across speci ...
(pdf-file 1,2 Mb)
... subunits were isolated from the Hrr25-depleted cells by sucrosegradient centrifugation and treated with a high salt concentration. Rps3 was released from the 40S subunit on incubation with 100 mM MgCl2, whereas most ribosomal proteins, including Rps8, were not dissociated (Fig. 4c). When 40S subunit ...
... subunits were isolated from the Hrr25-depleted cells by sucrosegradient centrifugation and treated with a high salt concentration. Rps3 was released from the 40S subunit on incubation with 100 mM MgCl2, whereas most ribosomal proteins, including Rps8, were not dissociated (Fig. 4c). When 40S subunit ...
Cytomechanical properties of papaver pollen tubes are altered after
... space of the cell, and second, what alterations in cytomechanical properties are stimulated. External signals triggering intracellular signaling cascades are usually perceived by transmembrane receptors whose localization may be distributed all over the cell surface or in specific regions of the cel ...
... space of the cell, and second, what alterations in cytomechanical properties are stimulated. External signals triggering intracellular signaling cascades are usually perceived by transmembrane receptors whose localization may be distributed all over the cell surface or in specific regions of the cel ...
Morphologic and functional characterization of granulocytes and
... Figure 1. Light microscope appearance of adult zebrafish granulocytes. (A) A heterophil (black arrow) and an eosinophil (arrowhead) in a single field of a May-Grünwald-Giemsa–stained zebrafish blood smear. (B) Detail of mature heterophils with double- and triple-lobed nuclei (May-Grünwald-Giemsa–s ...
... Figure 1. Light microscope appearance of adult zebrafish granulocytes. (A) A heterophil (black arrow) and an eosinophil (arrowhead) in a single field of a May-Grünwald-Giemsa–stained zebrafish blood smear. (B) Detail of mature heterophils with double- and triple-lobed nuclei (May-Grünwald-Giemsa–s ...
Mechanism of Uptake and Retrograde Axonal Transport of
... types of membrane during exocytotic transmitter release, vesicle membrane seems to be retrieved selectively (25, 26, 38, 67). Less clear is the origin of synaptic vesicles. Vesicles, especially large dense-core vesicles in adrenergic or serotoninergic neurons, are rapidly transported from the cell b ...
... types of membrane during exocytotic transmitter release, vesicle membrane seems to be retrieved selectively (25, 26, 38, 67). Less clear is the origin of synaptic vesicles. Vesicles, especially large dense-core vesicles in adrenergic or serotoninergic neurons, are rapidly transported from the cell b ...
Salivary gland development in Drosophila
... SCR-induced salivary fates are limited to more anterior segments when SCR is expressed everywhere, some downstream genes, such as fork head (fkh), are also induced in more posterior segments (Panzer et al., 1992). This observation suggests differences among salivary gland genes with respect to which ...
... SCR-induced salivary fates are limited to more anterior segments when SCR is expressed everywhere, some downstream genes, such as fork head (fkh), are also induced in more posterior segments (Panzer et al., 1992). This observation suggests differences among salivary gland genes with respect to which ...
The basic helix–loop–helix protein BETA2 interacts with p300 to
... very low levels alone or with coexpressed E47, a ubiquitous bHLH protein (Fig. 2A, columns 1,3). Cotransfecting increasing amounts of a p300 expression plasmid further potentiated BETA2-dependent transcription to >100-fold greater than the reporter alone. The failure of p300 to transactivate the rep ...
... very low levels alone or with coexpressed E47, a ubiquitous bHLH protein (Fig. 2A, columns 1,3). Cotransfecting increasing amounts of a p300 expression plasmid further potentiated BETA2-dependent transcription to >100-fold greater than the reporter alone. The failure of p300 to transactivate the rep ...
Speciation of Small Molecules and Inorganic Ions in Salmon Egg
... S O) by ICP-MS because 32S was interfered with the polyatomic ion of 16O16O. As can be seen in the chromatogram of S (Fig. 1), several peaks were observed in the large and small molecular regions, although the chromatogram was rather complicated with a poor signal intensity. The peaks of S in the sm ...
... S O) by ICP-MS because 32S was interfered with the polyatomic ion of 16O16O. As can be seen in the chromatogram of S (Fig. 1), several peaks were observed in the large and small molecular regions, although the chromatogram was rather complicated with a poor signal intensity. The peaks of S in the sm ...
Differential Subnuclear Localization of RNA Strands of Opposite
... ()-strand PSTVd RNAs observed in cultured N. benthamiana cells would mirror the situation in infected plants, we used FISH to detect the subcellular localization of PSTVd on cryosections of systemically infected leaves of tomato and N. benthamiana collected 4 to 5 weeks after inoculation. The exper ...
... ()-strand PSTVd RNAs observed in cultured N. benthamiana cells would mirror the situation in infected plants, we used FISH to detect the subcellular localization of PSTVd on cryosections of systemically infected leaves of tomato and N. benthamiana collected 4 to 5 weeks after inoculation. The exper ...
Accepted version - QMRO Home - Queen Mary University of London
... Although these data are based on comparisons of quite narrow ranges of donor cell survival (only <5% at the initial points studied), these may suggest that allogeneic MSCs can survive for a certain early period after transplantation, but are eventually rejected in a long term. We have recently repor ...
... Although these data are based on comparisons of quite narrow ranges of donor cell survival (only <5% at the initial points studied), these may suggest that allogeneic MSCs can survive for a certain early period after transplantation, but are eventually rejected in a long term. We have recently repor ...
11. Reaction Diffusion, Chemotaxis, and Nonlocal Mechanisms
... 11.1 Simple Random Walk and Derivation of the Diffusion Equation In an assemblage of particles, for example, cells, bacteria, chemicals, animals and so on, each particle usually moves around in a random way. The particles spread out as a result of this irregular individual particle’s motion. When th ...
... 11.1 Simple Random Walk and Derivation of the Diffusion Equation In an assemblage of particles, for example, cells, bacteria, chemicals, animals and so on, each particle usually moves around in a random way. The particles spread out as a result of this irregular individual particle’s motion. When th ...
The association of sore throat and psoriasis might be explained by
... © 2013 British Society for Immunology, Clinical and Experimental Immunology, 174: 139–151 ...
... © 2013 British Society for Immunology, Clinical and Experimental Immunology, 174: 139–151 ...