Chapter 7 Membrane Structure and Function Multiple
... 40) A patient has had a serious accident and lost a lot of blood. In an attempt to replenish body fluids, distilled water, equal to the volume of blood lost, is transferred directly into one of his veins. What will be the most probable result of this transfusion? A) It will have no unfavorable effec ...
... 40) A patient has had a serious accident and lost a lot of blood. In an attempt to replenish body fluids, distilled water, equal to the volume of blood lost, is transferred directly into one of his veins. What will be the most probable result of this transfusion? A) It will have no unfavorable effec ...
The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to
... 20% of the cells transfected with FIP3 siRNA#1 properly targeted Rh–GFP–VxPx (P53.36E24) (Fig. 1E). Treatment with siRNA#1 decreased FIP3 protein content to 20–40% of control levels (five separate experiments), while having negligible effect on the expression of ASAP1 (Fig. 1F). In experiments with ...
... 20% of the cells transfected with FIP3 siRNA#1 properly targeted Rh–GFP–VxPx (P53.36E24) (Fig. 1E). Treatment with siRNA#1 decreased FIP3 protein content to 20–40% of control levels (five separate experiments), while having negligible effect on the expression of ASAP1 (Fig. 1F). In experiments with ...
A tightly regulated inducible expression system for
... First-generation inducible expression vectors for Trypanosoma brucei utilized a single tetracycline-responsive promoter to drive expression of an experimental gene, in tandem with a drug-resistance marker gene to select for integration (Wirtz E, Clayton CE. Science 1995; 268:1179 – 1183). Because dr ...
... First-generation inducible expression vectors for Trypanosoma brucei utilized a single tetracycline-responsive promoter to drive expression of an experimental gene, in tandem with a drug-resistance marker gene to select for integration (Wirtz E, Clayton CE. Science 1995; 268:1179 – 1183). Because dr ...
Giant nuclei is essential in the cell cycle transition from meiosis to
... lacking the C-terminal 94 residues. The deduced Gnu sequence was used to search the protein databases. No close matches were found, therefore Gnu is a novel protein. Fig. 1. DNA replication in gnu embryos. (A-D) Fluorescently immunostained eggs and embryos from gnu homozygous mothers. A nuclear lami ...
... lacking the C-terminal 94 residues. The deduced Gnu sequence was used to search the protein databases. No close matches were found, therefore Gnu is a novel protein. Fig. 1. DNA replication in gnu embryos. (A-D) Fluorescently immunostained eggs and embryos from gnu homozygous mothers. A nuclear lami ...
Echinoderm enteric nervous system
... separation by basement membranes found for other ectoneural–hyponeural nervous system divisions in echinoderms (Cobb, 1987). The function of the catecholaminergic plexus remains a mystery. It has been suggested that the catecholaminergic neurons and fibres of the basiepithelial plexus are involved i ...
... separation by basement membranes found for other ectoneural–hyponeural nervous system divisions in echinoderms (Cobb, 1987). The function of the catecholaminergic plexus remains a mystery. It has been suggested that the catecholaminergic neurons and fibres of the basiepithelial plexus are involved i ...
The expression of a zebrafish gene homologous to Drosophila snail
... notochord (Halpern et al., 1993). The phenotype of ntl is very similar to that of mouse embryos mutant in the brachyury (T) gene (Chesley, 1935; Yanagisawa et al., 1981; Herrmann et al., 1990). The zebrafish homologue of the T gene was cloned and shown to be identical with the ntl gene (Schulte-Merk ...
... notochord (Halpern et al., 1993). The phenotype of ntl is very similar to that of mouse embryos mutant in the brachyury (T) gene (Chesley, 1935; Yanagisawa et al., 1981; Herrmann et al., 1990). The zebrafish homologue of the T gene was cloned and shown to be identical with the ntl gene (Schulte-Merk ...
Regulation of erythropoiesis
... pathway and rescue anemia (in trial). In patients with renal failure, in addition to hepatocytes, osteoblasts can also produce EPO to increase RBC production after PHD inhibition. ...
... pathway and rescue anemia (in trial). In patients with renal failure, in addition to hepatocytes, osteoblasts can also produce EPO to increase RBC production after PHD inhibition. ...
Here - New Mexico State University
... invite new looks at questions of comparative genomics ranging from the evolution of gene families to the form of the ancestral echinoderm genome. Furthermore, comparative sequence analyses previously possible with only isolated sequence regions such as BAC inserts can now be applied to whole genomes ...
... invite new looks at questions of comparative genomics ranging from the evolution of gene families to the form of the ancestral echinoderm genome. Furthermore, comparative sequence analyses previously possible with only isolated sequence regions such as BAC inserts can now be applied to whole genomes ...
Biology - Tutor
... organisms that live in extreme habitats, such as deep ocean steam vents. (The term prokaryotic is used to describe an organism that lacks the membrane-bounded nucleus and membranous organelles typical of eukaryotes.) 2. Domain Bacteria consists of prokaryotic, unicellular, bacteria. They inhabit a w ...
... organisms that live in extreme habitats, such as deep ocean steam vents. (The term prokaryotic is used to describe an organism that lacks the membrane-bounded nucleus and membranous organelles typical of eukaryotes.) 2. Domain Bacteria consists of prokaryotic, unicellular, bacteria. They inhabit a w ...
Co-Expression of Arabidopsis thaliana Phytochelatin Synthase and Treponema denticola
... hydrogen sulfide only under strictly anaerobic condition (White and Gadd, 1998) and therefore they are not suitable for applications under aerobic conditions. Wang et al. (2001) functionally expressed a cysteine desulfhydrase (CysDes) from Treponema denticola in E. coli for the aerobic sulfide product ...
... hydrogen sulfide only under strictly anaerobic condition (White and Gadd, 1998) and therefore they are not suitable for applications under aerobic conditions. Wang et al. (2001) functionally expressed a cysteine desulfhydrase (CysDes) from Treponema denticola in E. coli for the aerobic sulfide product ...
Thesis-1972D-H792p
... CHAPTER. I INTRODUCTION Because cell division is by its nature a very complex process, there are many different aspects of the phenomenon that could be investigated. ...
... CHAPTER. I INTRODUCTION Because cell division is by its nature a very complex process, there are many different aspects of the phenomenon that could be investigated. ...
Cell fate specification and maintenance in the female gametophyte
... (Friml et al., 2003). It was reported that the apical auxin maximum requires transport of auxin by the auxin efflux carrier PIN-FORMED 7 (PIN7), and the accumulation of auxin in the apical cell possibly together with other factors is required for the specification of the apical cell identity (Friml ...
... (Friml et al., 2003). It was reported that the apical auxin maximum requires transport of auxin by the auxin efflux carrier PIN-FORMED 7 (PIN7), and the accumulation of auxin in the apical cell possibly together with other factors is required for the specification of the apical cell identity (Friml ...
vascular tissue differentiation and pattern formation in plants
... and the induction rate of tracheary elements can reach up to 60%. Thus the biochemical and molecular changes associated with the differentiation of a single cell type, tracheary elements, can be monitored. A number of genes associated with tracheary element formation have been isolated and character ...
... and the induction rate of tracheary elements can reach up to 60%. Thus the biochemical and molecular changes associated with the differentiation of a single cell type, tracheary elements, can be monitored. A number of genes associated with tracheary element formation have been isolated and character ...
FURTHER STUDIES ON THE THETA CELL OF THE MOUSE
... Theta cells show dense aggregations of the endoplasmic reticulum at the cell periphery corresponding with the basophilic region observed by light microscopy (Fig. 1). In this area, membranebounded cavities of the endoplasmic reticulum are very flattened and extend to distant areas. The contents of t ...
... Theta cells show dense aggregations of the endoplasmic reticulum at the cell periphery corresponding with the basophilic region observed by light microscopy (Fig. 1). In this area, membranebounded cavities of the endoplasmic reticulum are very flattened and extend to distant areas. The contents of t ...
Decontamination of the BD FACSAria System
... for contamination. The sheath tank is removable and autoclavable. Some of the tubing is now made from Teflon®, and the new fluidics system requires fewer valves. Valves are a manifold style (with less dead volume than the original style) and are very reliable. The sheath fluid path has a dedicated f ...
... for contamination. The sheath tank is removable and autoclavable. Some of the tubing is now made from Teflon®, and the new fluidics system requires fewer valves. Valves are a manifold style (with less dead volume than the original style) and are very reliable. The sheath fluid path has a dedicated f ...
Localization of Phycoerythrin at the Lumenal Surface of the
... Epon resin. Other cells were dehydrated in 25% ethanol at -5°C, then in 50, 75, and 95% ethanol at -18"C. Infiltration and embedding in ~ i c r y l K4M at -18°C were performed according to the following schedule: 95% ethanol/resin, 1:1, overnight; 95% ethanol/resin, 1:2, twice for 4 h each and then ...
... Epon resin. Other cells were dehydrated in 25% ethanol at -5°C, then in 50, 75, and 95% ethanol at -18"C. Infiltration and embedding in ~ i c r y l K4M at -18°C were performed according to the following schedule: 95% ethanol/resin, 1:1, overnight; 95% ethanol/resin, 1:2, twice for 4 h each and then ...
Plant Cell Wall Proteins: A Large Body of Data, but What about
... Plant cell wall proteomics is a tricky field of research, since proteins are not only minor components of plant cell walls, but are also trapped in complex networks of polysaccharides with which they can interact. Plant cell walls are mainly composed of cellulose microfibrils wrapped in and connecte ...
... Plant cell wall proteomics is a tricky field of research, since proteins are not only minor components of plant cell walls, but are also trapped in complex networks of polysaccharides with which they can interact. Plant cell walls are mainly composed of cellulose microfibrils wrapped in and connecte ...
Zygotic Genome Activation During the Maternal-to
... In most animals, the maternal contribution directs a series of synchronized mitoses while maintaining a relatively constant volume as it forms a blastula. Subsequently, coordinated cell movements during gastrulation form distinct germ layers that specify the various tissues in the mature organism. W ...
... In most animals, the maternal contribution directs a series of synchronized mitoses while maintaining a relatively constant volume as it forms a blastula. Subsequently, coordinated cell movements during gastrulation form distinct germ layers that specify the various tissues in the mature organism. W ...
Cardioprotective Effects of Erythropoietin in Rats Subjected to
... anesthetized (sodium pentobarbital, 30 mg/kg intraperitoneally) for a baseline ventricular function measurement by echocardiography (Sonos 5500 equipped with a 15-MHz phased-array transducer; Philips Electronics N.V.). The anesthetized rats were subjected to 20 min of myocardial ischemia and 30 min ...
... anesthetized (sodium pentobarbital, 30 mg/kg intraperitoneally) for a baseline ventricular function measurement by echocardiography (Sonos 5500 equipped with a 15-MHz phased-array transducer; Philips Electronics N.V.). The anesthetized rats were subjected to 20 min of myocardial ischemia and 30 min ...
Evidence that non-caspase proteases are required for
... 1995), nuc-58 (Deng and Podack, 1995), DNase I (Peitsch et al, 1994), DNase II (Eastman, 1994), DNase g (Shiokawa et al, 1994), ILCME (Khodarev and Ashwell, 1996), a 25 Kd activity (Schwartzman and Cidlowski, 1993), a 97 Kd molecule (Pandey et al, 1997) and a caspase-activiated DNase (CAD) (Enari et ...
... 1995), nuc-58 (Deng and Podack, 1995), DNase I (Peitsch et al, 1994), DNase II (Eastman, 1994), DNase g (Shiokawa et al, 1994), ILCME (Khodarev and Ashwell, 1996), a 25 Kd activity (Schwartzman and Cidlowski, 1993), a 97 Kd molecule (Pandey et al, 1997) and a caspase-activiated DNase (CAD) (Enari et ...
Chromosome organization and dynamics during
... development of the concept of “transcriptional factories,” discrete sites in the nucleus where gene transcription is particularly active (Sutherland and Bickmore, 2009). Hundreds of such factories are proposed to be present in each nucleus and they are thought to be anchored to a nuclear substructur ...
... development of the concept of “transcriptional factories,” discrete sites in the nucleus where gene transcription is particularly active (Sutherland and Bickmore, 2009). Hundreds of such factories are proposed to be present in each nucleus and they are thought to be anchored to a nuclear substructur ...
Naive CD8 T cells differentiate into protective memory
... naive OT-I cells underwent three to four divisions in lethally irradiated WT hosts after rat IgG treatment, because of lymphopenia-induced HP. Proliferation was delayed in MHC class Ia–deficient hosts, confirming that HP of naive CD8 T cells is MHC class I–dependent (22–24). In contrast, IL-2 signa ...
... naive OT-I cells underwent three to four divisions in lethally irradiated WT hosts after rat IgG treatment, because of lymphopenia-induced HP. Proliferation was delayed in MHC class Ia–deficient hosts, confirming that HP of naive CD8 T cells is MHC class I–dependent (22–24). In contrast, IL-2 signa ...
Golgi-targeting sequence of p230 - Journal of Cell Science
... mechanism to target this peripheral membane protein specifically to TGN membranes. The majority (approx. 85%) of p230 is predicted to form an α-helical coil-coiled structure, notable exceptions being the 120- and 80-amino acid amino and carboxy termini (Erlich et al., 1996) (Fig. 1). To examine the ...
... mechanism to target this peripheral membane protein specifically to TGN membranes. The majority (approx. 85%) of p230 is predicted to form an α-helical coil-coiled structure, notable exceptions being the 120- and 80-amino acid amino and carboxy termini (Erlich et al., 1996) (Fig. 1). To examine the ...