Responses to Cell Loss Become Restricted as the Supporting Cells
... Figure 2. LatA treatments did not produce detectable changes in the size of the reinforced F-actin bands in adult mouse utricles. A, Confocal images of sensory epithelia (SE) that were cultured with 5 M LatA or vehicle control for 1, 12, or 24 h and then fixed and labeled with fluorescent phalloidi ...
... Figure 2. LatA treatments did not produce detectable changes in the size of the reinforced F-actin bands in adult mouse utricles. A, Confocal images of sensory epithelia (SE) that were cultured with 5 M LatA or vehicle control for 1, 12, or 24 h and then fixed and labeled with fluorescent phalloidi ...
151 Compact cell image projector
... contraction amplitude of isolated rat ventricular myocytes. Peak cell shortening amplitude was recorded during electric stimulation at the average rate of 0.5 Hz with different stimulatory patterns: regular and pseudo-random rhythms, as well as double pulse stimulation. Cells were perfused at 23 ºC ...
... contraction amplitude of isolated rat ventricular myocytes. Peak cell shortening amplitude was recorded during electric stimulation at the average rate of 0.5 Hz with different stimulatory patterns: regular and pseudo-random rhythms, as well as double pulse stimulation. Cells were perfused at 23 ºC ...
Tissue Engineered Human Skin Equivalents
... intact epithelium sheets using protease Dispase. An estimation of 6000-fold increase in size of total harvest epithelium as compared to that of the initial skin biopsy was made accordingly [11]. Rheinwald and Green’s work provided the method to obtain sufficient keratinocytes for (large-scale) bioen ...
... intact epithelium sheets using protease Dispase. An estimation of 6000-fold increase in size of total harvest epithelium as compared to that of the initial skin biopsy was made accordingly [11]. Rheinwald and Green’s work provided the method to obtain sufficient keratinocytes for (large-scale) bioen ...
Plant Gravitropism. Unraveling the Ups and
... exhibit upward bending when reoriented horizontally. Like the columella and endodermal cells of higher plants, C. purpureus protonemata contain amyloplasts that sediment with respect to the gravity vector. If gravity sensing were dependent on the mass of the cell as proposed by the gravitational pre ...
... exhibit upward bending when reoriented horizontally. Like the columella and endodermal cells of higher plants, C. purpureus protonemata contain amyloplasts that sediment with respect to the gravity vector. If gravity sensing were dependent on the mass of the cell as proposed by the gravitational pre ...
SEDS proteins are a widespread family of bacterial
... drive cell elongation, while the divisome synthesizes peptidoglycan during cytokinesis generating the incipient cell poles3. Notably, a small subset of bacteria that possess peptidoglycan lack aPBPs and do not encode other proteins with GT51 domains4. Furthermore, the model Gram-positive bacterium B ...
... drive cell elongation, while the divisome synthesizes peptidoglycan during cytokinesis generating the incipient cell poles3. Notably, a small subset of bacteria that possess peptidoglycan lack aPBPs and do not encode other proteins with GT51 domains4. Furthermore, the model Gram-positive bacterium B ...
Characteristics of Bacteria Worksheet
... energy the consumers in the ecosystem can use. 3. The third group is the chemosynthetic autotrophs. This group can make their own energy but instead of using sunlight to do it they use chemicals around them. These bacteria are important in changing the nitrogen in the atmosphere that we can’t use in ...
... energy the consumers in the ecosystem can use. 3. The third group is the chemosynthetic autotrophs. This group can make their own energy but instead of using sunlight to do it they use chemicals around them. These bacteria are important in changing the nitrogen in the atmosphere that we can’t use in ...
Role of N-linked oligosaccharide chains in the processing and
... (Sambrook et al., 1989). Oligonucleotide-directed site-specific mutagenesis was performed using a kit according to the instructions of the supplier (Bio-Rad). To obtain mutant cDNA lacking a particular single natural consensus site, we performed oligonucleotide-directed mutagenesis using a single ol ...
... (Sambrook et al., 1989). Oligonucleotide-directed site-specific mutagenesis was performed using a kit according to the instructions of the supplier (Bio-Rad). To obtain mutant cDNA lacking a particular single natural consensus site, we performed oligonucleotide-directed mutagenesis using a single ol ...
PAK1 Mediates Resistance to PI3 Kinase Inhibition in Lymphomas
... Downloaded from clincancerres.aacrjournals.org on June 15, 2017. © 2013 American Association for Cancer Research. ...
... Downloaded from clincancerres.aacrjournals.org on June 15, 2017. © 2013 American Association for Cancer Research. ...
Mesoderm commitment to hematopoiesis - Development
... Scott M. Robertson1, Marion Kennedy2, John M. Shannon3 and Gordon Keller2,* 1Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390, USA 2Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, 3Division of Pulmonary Biology, Children’s ...
... Scott M. Robertson1, Marion Kennedy2, John M. Shannon3 and Gordon Keller2,* 1Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390, USA 2Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, 3Division of Pulmonary Biology, Children’s ...
Antibody Binding to Cryptococcus neoformans Impairs Budding by
... away from the cell. Examples of such direct antimicrobial effects include alterations of microbial metabolic activity, gene expression, quorum sensing, and susceptibility to drugs (2, 3). The physical mechanism(s) of such direct Ab-mediated effects upon capsule binding are poorly understood. The stu ...
... away from the cell. Examples of such direct antimicrobial effects include alterations of microbial metabolic activity, gene expression, quorum sensing, and susceptibility to drugs (2, 3). The physical mechanism(s) of such direct Ab-mediated effects upon capsule binding are poorly understood. The stu ...
Replacement of threonine residues by serine and alanine in a
... inhibits thick filament formation and reduces the actin-activated Mg2 + -ATPase activity of the myosin [4,5]. These effects suggest an involvement of myosin phosphorylation in the control of cell motility during the chemotactic response. In mutants lacking myosin II the average speed of cell movemen ...
... inhibits thick filament formation and reduces the actin-activated Mg2 + -ATPase activity of the myosin [4,5]. These effects suggest an involvement of myosin phosphorylation in the control of cell motility during the chemotactic response. In mutants lacking myosin II the average speed of cell movemen ...
Exclusion of Ribulose-1,5-bisphosphate Carboxylase/oxygenase
... Minamikawa et al. (2001) have reported a similar phenomenon in French bean leaves that were detached and placed in darkness. On the other hand, many previous reports have shown that the decrease of Rubisco content is much faster than the decrease in the number of chloroplasts in leaves during natura ...
... Minamikawa et al. (2001) have reported a similar phenomenon in French bean leaves that were detached and placed in darkness. On the other hand, many previous reports have shown that the decrease of Rubisco content is much faster than the decrease in the number of chloroplasts in leaves during natura ...
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and
... (unmodified) OPN to identify MAbs that specifically recognize either the native or the recombinant form. Antibodies specific only for the native form would be candidates for MAbs recognizing an epitope that is post-translationally modified; antibodies specific only for the recombinant form would pre ...
... (unmodified) OPN to identify MAbs that specifically recognize either the native or the recombinant form. Antibodies specific only for the native form would be candidates for MAbs recognizing an epitope that is post-translationally modified; antibodies specific only for the recombinant form would pre ...
What do colony patterns mean? - James A. Shapiro
... 1. Amino acids necessary (I.B. Holland, personal communication) 2. DBM limited to a special region of the nutritional-mobility space 3. DBM characterized by branches that do not grow in width 4. Tip-splitting occurs when branches separated by a critical distance 5. Increased cell activity in a limit ...
... 1. Amino acids necessary (I.B. Holland, personal communication) 2. DBM limited to a special region of the nutritional-mobility space 3. DBM characterized by branches that do not grow in width 4. Tip-splitting occurs when branches separated by a critical distance 5. Increased cell activity in a limit ...
Thinking about Bacillus subtilis as a multicellular organism
... fluorescent protein in developing colonies [15]. Unfortunately, in both these approaches the magnification levels that were utilized did not allow visualization of individual cells, so it is still not known if all the cells in the areas where sporulation is observed are indeed undergoing sporulatio ...
... fluorescent protein in developing colonies [15]. Unfortunately, in both these approaches the magnification levels that were utilized did not allow visualization of individual cells, so it is still not known if all the cells in the areas where sporulation is observed are indeed undergoing sporulatio ...
TRYPSIN SYNTHESIS AND STORAGE AS ZYMOGEN IN THE
... synthesized TAP or when TAP-antibodies were tested against synthesized IP. Control sera reacted negatively against both synthesized IP and TAP. Both TAP and IP-antibodies reacted negatively when tested with bovine chymotrypsin. The SDS-PAGE of midgut gland extract showed a complex protein compositio ...
... synthesized TAP or when TAP-antibodies were tested against synthesized IP. Control sera reacted negatively against both synthesized IP and TAP. Both TAP and IP-antibodies reacted negatively when tested with bovine chymotrypsin. The SDS-PAGE of midgut gland extract showed a complex protein compositio ...
Membrane traffic and fusion at post-Golgi compartments
... with a Golgi stack but can also move away (Viotti et al., 2010; Kang et al., 2011). The TGN is a major sorting station for exocytic cargo proteins except that some storage proteins are sorted at the ER or cis-Golgi (Hara-Nishimura et al., 1998; Park et al., 2004). Importantly, the TGN also functions ...
... with a Golgi stack but can also move away (Viotti et al., 2010; Kang et al., 2011). The TGN is a major sorting station for exocytic cargo proteins except that some storage proteins are sorted at the ER or cis-Golgi (Hara-Nishimura et al., 1998; Park et al., 2004). Importantly, the TGN also functions ...
Ultrastructural immunocytochemical localization of
... et al. 1981; Repesh, Furcht & Smith, 1981; Silver et al. 1981; Thesleff et al 1981). The present results are of particular interest in view of the early stages in the development of the basal lamina used here. Fibronectin was found in this structure shortly after the time of laying with little diffe ...
... et al. 1981; Repesh, Furcht & Smith, 1981; Silver et al. 1981; Thesleff et al 1981). The present results are of particular interest in view of the early stages in the development of the basal lamina used here. Fibronectin was found in this structure shortly after the time of laying with little diffe ...
View PDF - G3: Genes | Genomes | Genetics
... 10, relative to neighboring cultures (Figure 2A). This is of interest because Rif1 and Rif2 have well-known roles in telomere biology, encoding proteins which bind via Rap1 to telomeric DNA, and are known to affect telomere capping (Addinall et al. 2011; Anbalagan et al. 2011; Chang et al. 2011). In ...
... 10, relative to neighboring cultures (Figure 2A). This is of interest because Rif1 and Rif2 have well-known roles in telomere biology, encoding proteins which bind via Rap1 to telomeric DNA, and are known to affect telomere capping (Addinall et al. 2011; Anbalagan et al. 2011; Chang et al. 2011). In ...
Two highly related regulatory subunits of PP2A exert opposite
... when two distinct morpholino oligonucleotides were used (data not shown), and was rescued by co-expression of a mouse Bδ mRNA (Fig. 1B). To understand how specific these effects were for this particular B subunit, we investigated the effects of manipulating levels of the highly homologous Bα, which ...
... when two distinct morpholino oligonucleotides were used (data not shown), and was rescued by co-expression of a mouse Bδ mRNA (Fig. 1B). To understand how specific these effects were for this particular B subunit, we investigated the effects of manipulating levels of the highly homologous Bα, which ...
Evaluation of Different Solvent Types on the Extraction of
... essential amino acids which are mostly equivalent or even better with that of other higher plant (Spolaore et al., 2006). It was found that their amino acid composition does not significantly affected by changes in environmental conditions (Blackburn and Volkman, 2012). Apart from that, the farming ...
... essential amino acids which are mostly equivalent or even better with that of other higher plant (Spolaore et al., 2006). It was found that their amino acid composition does not significantly affected by changes in environmental conditions (Blackburn and Volkman, 2012). Apart from that, the farming ...
Hopanoids Play a Role in Membrane Integrity and pH
... prevalence in ancient marine petroleum source rocks, they were proposed as biomarkers not only for cyanobacteria, but also for oxygenic photosynthesis (49, 56). However, there is no evidence that functionally links 2-methylbacteriohopanepolyols (2-MeBHPs) with oxygenic photosynthesis in modern cyano ...
... prevalence in ancient marine petroleum source rocks, they were proposed as biomarkers not only for cyanobacteria, but also for oxygenic photosynthesis (49, 56). However, there is no evidence that functionally links 2-methylbacteriohopanepolyols (2-MeBHPs) with oxygenic photosynthesis in modern cyano ...
Chemistry in living systems
... Rideout and coworkers recognized the potential use of ketones and aldehydes for chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent cap ...
... Rideout and coworkers recognized the potential use of ketones and aldehydes for chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent cap ...
05_Microb_biofilm_I_2014
... divergent – continuous layer of cells forms convergent – aggregates develop, even of different species (e.g. coaggregation of Streptococcus gordonii + Fusobacterium nucleatum in dental plaque) ...
... divergent – continuous layer of cells forms convergent – aggregates develop, even of different species (e.g. coaggregation of Streptococcus gordonii + Fusobacterium nucleatum in dental plaque) ...
The human uncoupling protein-3 gene promoter muscle cells
... requirement of the UCP-3 gene promoter for MyoD may be responsible for the preferential expression of the gene in differentiated muscle cells and its skeletal muscle-specifc expression in vivo (Fig. 3). The finding that RAR enhanced the responsiveness of the UCP-3 gene promoter to all-trans RA where ...
... requirement of the UCP-3 gene promoter for MyoD may be responsible for the preferential expression of the gene in differentiated muscle cells and its skeletal muscle-specifc expression in vivo (Fig. 3). The finding that RAR enhanced the responsiveness of the UCP-3 gene promoter to all-trans RA where ...