The human uncoupling protein-3 gene promoter muscle cells
... requirement of the UCP-3 gene promoter for MyoD may be responsible for the preferential expression of the gene in differentiated muscle cells and its skeletal muscle-specifc expression in vivo (Fig. 3). The finding that RAR enhanced the responsiveness of the UCP-3 gene promoter to all-trans RA where ...
... requirement of the UCP-3 gene promoter for MyoD may be responsible for the preferential expression of the gene in differentiated muscle cells and its skeletal muscle-specifc expression in vivo (Fig. 3). The finding that RAR enhanced the responsiveness of the UCP-3 gene promoter to all-trans RA where ...
Research Paper Role of Particle Size in Phagocytosis of Polymeric
... of a Langmuir equilibrium constant defined as K ¼ ð1Þ*½P : Where θ is the fraction of cell area occupied by attached particles and [P] is the concentration of unbound particles. Since a large excess of particles were used during attachment (10–300 particles still in suspension for every attached ...
... of a Langmuir equilibrium constant defined as K ¼ ð1Þ*½P : Where θ is the fraction of cell area occupied by attached particles and [P] is the concentration of unbound particles. Since a large excess of particles were used during attachment (10–300 particles still in suspension for every attached ...
SNARE complexes of different composition jointly
... addition to the SNARE domain and a C-terminal hydrophobic anchor (Rossi et al., 2004; Uemura et al., 2005; Lipka et al., 2007). The fusion of vesicles with the plasma membrane is generally mediated by the formation of a trimeric SNARE complex, consisting of a Qa-SNARE/syntaxin, a SNAP25-like SNARE ( ...
... addition to the SNARE domain and a C-terminal hydrophobic anchor (Rossi et al., 2004; Uemura et al., 2005; Lipka et al., 2007). The fusion of vesicles with the plasma membrane is generally mediated by the formation of a trimeric SNARE complex, consisting of a Qa-SNARE/syntaxin, a SNAP25-like SNARE ( ...
A Discussion of the Mechanism of Action of Steroid Hormones*
... aspects of hormone action will be discussed; steroid hormones will be presented as a molecular class of compounds, and some of the characteristics of the biological responses will be related for representa tive hormones. Turning to units of cellular struc ture and function, a discussion of basic con ...
... aspects of hormone action will be discussed; steroid hormones will be presented as a molecular class of compounds, and some of the characteristics of the biological responses will be related for representa tive hormones. Turning to units of cellular struc ture and function, a discussion of basic con ...
Optical Imaging Tools for Early Detection
... Hepatocellular carcinoma derived from triple transgenic mouse: Tet-O-Myc x LAP-tTA x L2G85 (LAP--Liver activator protein) ...
... Hepatocellular carcinoma derived from triple transgenic mouse: Tet-O-Myc x LAP-tTA x L2G85 (LAP--Liver activator protein) ...
Microfabrication in Biology and Medicine
... applications to biology or medicine during that time, only in the past decade has a closer union emerged. Several factors have driven this recent fusion. Commercially, high-throughput, low-volume-consumption technologies such as wholegenome sequencing projects and drug discovery have created a need ...
... applications to biology or medicine during that time, only in the past decade has a closer union emerged. Several factors have driven this recent fusion. Commercially, high-throughput, low-volume-consumption technologies such as wholegenome sequencing projects and drug discovery have created a need ...
The UBA2 Domain Functions as an Intrinsic Stabilization Signal that
... and UBA2 domains in Rad23 are indicated. (B) Lysates of 10 OD600 units of log-phase yeast coexpressing Ub-R-GFP-Rad23⌬UbL and 3HAubiquitin were subjected to immunoprecipitation with an anti-GFP antibody, followed by Western blotting with an anti-HA antibody. Yeast cells transformed with an empty vec ...
... and UBA2 domains in Rad23 are indicated. (B) Lysates of 10 OD600 units of log-phase yeast coexpressing Ub-R-GFP-Rad23⌬UbL and 3HAubiquitin were subjected to immunoprecipitation with an anti-GFP antibody, followed by Western blotting with an anti-HA antibody. Yeast cells transformed with an empty vec ...
Database Machine
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
Aminoacyl-tRNA synthetase complexes: beyond translation
... transduction (Ramakrishnan and White, 1998) and protein degradation (Glickman et al., 1998) is well established, and we know much about the regulation of the individual components. Several lines of evidence have suggested that the translational apparatus in mammalian cells is highly organized. In pa ...
... transduction (Ramakrishnan and White, 1998) and protein degradation (Glickman et al., 1998) is well established, and we know much about the regulation of the individual components. Several lines of evidence have suggested that the translational apparatus in mammalian cells is highly organized. In pa ...
A molecular framework for the inhibition of
... Information Fig. S1). We observed that 5 mm BA drastically inhibits root growth in comparison with the other solutions and conclude that the phenotypes shown in Fig. 1a,b are genuinely associated with boron toxicity. Root growth depends on the production of new cells, and their subsequent differenti ...
... Information Fig. S1). We observed that 5 mm BA drastically inhibits root growth in comparison with the other solutions and conclude that the phenotypes shown in Fig. 1a,b are genuinely associated with boron toxicity. Root growth depends on the production of new cells, and their subsequent differenti ...
1 Bacteria and Archaea
... and change it to a form that plants can use. Nitrogen from the air passes into the soil. Bacteria in the soil and on plant roots change nitrogen to another form. Plants use this form. Animals eat the plants to get nitrogen. Possible answers: Bacteria in soil turn nitrogen into a form that plants can ...
... and change it to a form that plants can use. Nitrogen from the air passes into the soil. Bacteria in the soil and on plant roots change nitrogen to another form. Plants use this form. Animals eat the plants to get nitrogen. Possible answers: Bacteria in soil turn nitrogen into a form that plants can ...
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in
... Connexin gene family, are crucially important in regulating embryonic development, coordinated contraction of excitable cells, tissue homeostasis, normal cell growth and differentiation (Saez et al., 2003; Sohl and Willecke, 2004). Furthermore, connexin mutations have been linked to several diseases ...
... Connexin gene family, are crucially important in regulating embryonic development, coordinated contraction of excitable cells, tissue homeostasis, normal cell growth and differentiation (Saez et al., 2003; Sohl and Willecke, 2004). Furthermore, connexin mutations have been linked to several diseases ...
letters - Cytomorpholab
... The endosperm in the Arabidopsis plant seed provides a simple and tractable system for studying cytokinesis within a multinucleate single cell10. Endosperm development involves a series of successive nuclear divisions that are devoid of cytokinesis, generating a syncytium11,12. After eight rounds of ...
... The endosperm in the Arabidopsis plant seed provides a simple and tractable system for studying cytokinesis within a multinucleate single cell10. Endosperm development involves a series of successive nuclear divisions that are devoid of cytokinesis, generating a syncytium11,12. After eight rounds of ...
Cellular Biology - Circulation Research
... trigger apoptosis or uncontrolled proliferation.4 Such a program might enable cardiomyocytes to adapt to different conditions such as hypertrophic growth, which will help to avoid potentially dangerous effects of cell divisions in the working myocardium that has to maintain a high blood pressure dur ...
... trigger apoptosis or uncontrolled proliferation.4 Such a program might enable cardiomyocytes to adapt to different conditions such as hypertrophic growth, which will help to avoid potentially dangerous effects of cell divisions in the working myocardium that has to maintain a high blood pressure dur ...
The water of life: osmoregulation in Saccharomyces cerevisiae
... Interactions between the target of rapamycin (TOR) and the CWI pathways during nutrient stress, and effects on osmotolerance ...
... Interactions between the target of rapamycin (TOR) and the CWI pathways during nutrient stress, and effects on osmotolerance ...
Modeling host interactions with hepatitis B virus using
... infection in induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (iHeps) (15, 16). These cells have demonstrated their utility for modeling inherited metabolic disorders (17), incorporating genetic manipulations (18), and supporting infection with HCV (19–21). During iHep generation, ...
... infection in induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (iHeps) (15, 16). These cells have demonstrated their utility for modeling inherited metabolic disorders (17), incorporating genetic manipulations (18), and supporting infection with HCV (19–21). During iHep generation, ...
TrueORFs - OriGene
... vectors. Most subcloning from the Entry to a destination vector involves Sgf I/Asis I (present in 0.37% of human ORF) and Mlu I (4%). In the very unusual case when Sgf I and Mlu I sites are inside the ORFs, the TrueORF vector MCS provides other rare restriction sites, such as Asc I, Rsr II, and Not ...
... vectors. Most subcloning from the Entry to a destination vector involves Sgf I/Asis I (present in 0.37% of human ORF) and Mlu I (4%). In the very unusual case when Sgf I and Mlu I sites are inside the ORFs, the TrueORF vector MCS provides other rare restriction sites, such as Asc I, Rsr II, and Not ...
Proper Folding and Endoplasmic Reticulum to Golgi
... albumin/PBS. Indirect immunofluorescence was visualized with an inverted Bio-Rad MRC-600 laser confocal microscope system. Images were processed with Bio-Rad Confocal Assistant software. Trypsin Digestion—Whole cell lysates (4 g protein/l in 2% CHAPS lysis buffer without protease inhibitors) were ...
... albumin/PBS. Indirect immunofluorescence was visualized with an inverted Bio-Rad MRC-600 laser confocal microscope system. Images were processed with Bio-Rad Confocal Assistant software. Trypsin Digestion—Whole cell lysates (4 g protein/l in 2% CHAPS lysis buffer without protease inhibitors) were ...
Contribution of microtubule growth polarity and flux to spindle
... or perpendicular to the spindle axis (xt-plot, Fig. 3A), from the time-lapse measurements (xyt). Visual inspection of movies and these constructed kymographs revealed that metaphase plant chromosomes (seen as a negative stain) maintained their positions without appreciable movements in x or y direct ...
... or perpendicular to the spindle axis (xt-plot, Fig. 3A), from the time-lapse measurements (xyt). Visual inspection of movies and these constructed kymographs revealed that metaphase plant chromosomes (seen as a negative stain) maintained their positions without appreciable movements in x or y direct ...
Cytoskeletal Motors in Arabidopsis. Sixty
... Kar3p kinesin from S. cerevisiae (Reddy and Day, 2001b), so they belong to the NCD/Kinesin-14 subfamily. KATA/ATK1 has been shown as a nonprocessive microtubule minus end-directed motor (Marcus et al., 2002). The Arabidopsis KATA/ATK1 protein plays a critical role in microtubule organization at the ...
... Kar3p kinesin from S. cerevisiae (Reddy and Day, 2001b), so they belong to the NCD/Kinesin-14 subfamily. KATA/ATK1 has been shown as a nonprocessive microtubule minus end-directed motor (Marcus et al., 2002). The Arabidopsis KATA/ATK1 protein plays a critical role in microtubule organization at the ...
Session 238 Lens development and molecular cell biology
... Hiroshi Sasaki, Eri Kubo. Ophthalmology, Kanazawa Medical University, Ishikawa, Japan. Purpose: Involvement of cytokines including TGFβ2 and FGF2 has been reported in the onset of postoperative posterior capsule opacity (PCO). Previously, we showed that expression level of proteoglycan decorin (DCN) ...
... Hiroshi Sasaki, Eri Kubo. Ophthalmology, Kanazawa Medical University, Ishikawa, Japan. Purpose: Involvement of cytokines including TGFβ2 and FGF2 has been reported in the onset of postoperative posterior capsule opacity (PCO). Previously, we showed that expression level of proteoglycan decorin (DCN) ...
Chapter 7 Membrane Structure and Function Multiple
... 40) A patient has had a serious accident and lost a lot of blood. In an attempt to replenish body fluids, distilled water, equal to the volume of blood lost, is transferred directly into one of his veins. What will be the most probable result of this transfusion? A) It will have no unfavorable effec ...
... 40) A patient has had a serious accident and lost a lot of blood. In an attempt to replenish body fluids, distilled water, equal to the volume of blood lost, is transferred directly into one of his veins. What will be the most probable result of this transfusion? A) It will have no unfavorable effec ...
Bone Matrix
... cell cycle and cell growth regulated genes that support proliferation During this proliferation period, and fundamental to development of bone phenotype, several genes associated with formation of the ECM (type I collagen, fibronectin, and transforming growth factor- (TGF- ) are actively expressed ...
... cell cycle and cell growth regulated genes that support proliferation During this proliferation period, and fundamental to development of bone phenotype, several genes associated with formation of the ECM (type I collagen, fibronectin, and transforming growth factor- (TGF- ) are actively expressed ...