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ExamplePoster3 - Bridgewater College
ExamplePoster3 - Bridgewater College

... FACTORS INFLUENCING THE SYNTHESIS OF POLYHYDROXYBUTYRATE DEPOLYMERASE IN STREPTOMYCES SP. 5A Matthew B. Persinger, Matthew Shull, and Stephen F. Baron, Biology Dept., Bridgewater College, Bridgewater, VA 22812 ...
Expression of and Cytokine Activation by Eschevichia coli Curi
Expression of and Cytokine Activation by Eschevichia coli Curi

... Reprints or correspondence: Dr. Zhao Bian, Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, 171 77 Stockholm, Sweden ...
Ilyas R et al. High Glucose Disrupts Oligosaccharide Recognition
Ilyas R et al. High Glucose Disrupts Oligosaccharide Recognition

... Defining characteristics of diabetes mellitus (DM) are the elevated concentrations of free glucose in the circulation and body fluids. Lowering of glucose levels alleviates the pathological symptoms of the disease and improves the prognosis of the patients. In addition to perpetuating metabolic dist ...
Extrapolation of elementary rate constants of P
Extrapolation of elementary rate constants of P

... measured using the SDS software of the ABI PrismTM 7900HT Sequence Detection System (TaqManTM) starting with 10 minutes at 95 °C, followed by 40 cycles of 15 seconds at 95 °C and 1 minute at 60 °C. Each sample was analyzed in triplicate. The targeted mRNA in MDCKII-hMDR1NKI and Caco-2 cells was norm ...
The SR Protein SRp38 Represses Splicing in M Phase Cells
The SR Protein SRp38 Represses Splicing in M Phase Cells

... in gene expression, is inhibited in extracts of mitotic cells. Strikingly, SRp38 was found to be dephosphorylated specifically in mitotic cells, and we show that dephosphorylated SRp38 is required for the observed splicing repression. Introduction Splicing of mRNA precursors is not only a nearly ubi ...
Xenopus ADAM13 and ADAM19 are Important for Proper
Xenopus ADAM13 and ADAM19 are Important for Proper

... Some of the unpublished work discussed in this thesis focuses on the roles of ADAM13 and ADAM19 in the dorsal mesoderm during gastrulation. Specific emphasis is made on investigating the axial mesoderm during notochord formation. I show that ADAM19 affects gene expression important for the A-P polar ...
LY-294002-inhibitable PI 3-kinase and regulation of baseline rates
LY-294002-inhibitable PI 3-kinase and regulation of baseline rates

... respectively. It can be observed that the response to LY-294002 was more rapid in unstimulated tissues treated with the higher 50 ␮M concentration of LY294002 than with 10 ␮M LY-294002 in unstimulated and aldosterone-prestimulated tissues. Inhibition of Isc by LY-294002 was completely reversible wit ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified

The Development of Radial and Biradial Symmetry: The Evolution of
The Development of Radial and Biradial Symmetry: The Evolution of

... by a largely acellular mesoglea. The cnidarians generally have multiple planes of mirror symmetry which pass through the major longitudinal axis, the oral-aboral axis. A. Cross section of the benthic polyp form which attaches to the substrate via the basal discs located at the aboral pole. Numerous ...
Production and Characterization of Islet Amyloid Polypeptide Using
Production and Characterization of Islet Amyloid Polypeptide Using

... body [6]. At physiological amount, amylin behaves like a growth factor which contributes to bone calcification [6], thereby preventing the resorptive activities of osteoclasts [7]. Amylin regulates nutrient uptake and metabolism. It does so by diminishing the amount of food absorbed, gastric acid re ...
- Wiley Online Library
- Wiley Online Library

... planes to form a globular embryo proper (Figure 1b) (Mansfield and Briarty, 1991; Jürgens and Mayer, 1994). These two cell lineages differ not only in division plane orientation but also in gene expression and prospective cell fate. Several proteins such as GNOM, YODA (YDA), MITOGENACTIVATED PROTEI ...
Identification of an H Antigen-like Blood Group
Identification of an H Antigen-like Blood Group

... buffer, pH 7.0) for l h at 4°C.The membrane fragments were obtained by ultracentrifugation at 28,000 x g for 30 min, and then the membrane pellets were treated with a detergent buffer [10 HIMTris-HCl, 150 mM NaCl, pH 7.4, containing 0.5% NP-40 and 1 mM phenylmethylsulfonyl fluoride, Sigma] for 30 m ...
FERRITIN: A POTENT INHIBITOR OF VASCULAR CALCIFICATION
FERRITIN: A POTENT INHIBITOR OF VASCULAR CALCIFICATION

... role in heme catabolism and erythrocyte turnover, it has become increasingly evident that HO-1, plays an important protective role in numerous injury settings and clinical conditions. The mechanism underlying this cytoprotective effect involves the ability of HO-1 to catabolize free heme, which is ...
INSULIN RESISTANCE IN H-35 RAT HEPATO~A CELLS IS
INSULIN RESISTANCE IN H-35 RAT HEPATO~A CELLS IS

... maximal response. The concentration of insulin (measured by radioimmunoassay) remaining in the medium after 24 h of incubation with H-35 cells is 150 ng/ml, which is more than the maximally effective concentration of 20 ng/ml (see Fig. 2, upper curve). At 24 h (Fig. IB) the medium was removed from t ...
PDF + SI - Development - The Company of Biologists
PDF + SI - Development - The Company of Biologists

... functional equivalent of the heart, to the lateral epidermis. AMs were identified some years ago in Drosophila (Rizki, 1978), but have largely escaped attention. The name alary refers to a wing-like (aliform) shape. Sparse descriptions of AMs in adults of different insect species led to proposals of ...
Specific Innervation of Neurons in the Paravertebral
Specific Innervation of Neurons in the Paravertebral

... synaptic neurons are recognized. Although several hypotheses on the mechanism of selective synaptogenesis in the sympathetic nervous system have been proposed (see, for example, Rubin, 1985a-c), the ability to test these hypotheses is limited by the inaccessibility of the mammalian embryo. In the pr ...
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts

... the organellar proteome of Arabidopsis has identified four different VSRs at three separate locations by principal components analysis. One of them is very close to a V0a subunit of a V-ATPase subunit, pointing to a TGN location (Dunkley et al., 2006). This suggests a functional specialization of di ...
Characterization of the Distal Polyadenylation Site of the ß
Characterization of the Distal Polyadenylation Site of the ß

... A1, A23 and A4) and their respective flanking regions [17]. These regions showed to be highly conserved among species, supporting the idea that the most important regulatory elements of polyadenylation reside within those regions (Figure 1A and Figures S1, S2 and S3). The generated A1-A23-A4 constru ...
Glucocorticoids Regulate Pituitary Growth Hormone
Glucocorticoids Regulate Pituitary Growth Hormone

... various doses of dexamethasone, was monitored by RT-PCR (Fig. 2). GHRH-R mRNA levels were significantly elevated by all doses of dexamethasone (P , 0.0004), with a maximal increase of approximately 5-fold in the presence of 100 nm dexamethasone. Using the maximally effective dose of 100 nm dexametha ...
Second Messengers Mediate Increases in Cytosolic Calcium in
Second Messengers Mediate Increases in Cytosolic Calcium in

... Ca21 entering the cell down a concentration gradient. Receptor activation, at least in mammalian cells, can trigger the phosphoinositide cascade, which leads to the production of IP3 and 1,2-diacylglycerol (Berridge and Irvine, 1984). IP3 can then provoke the release from the internal Ca21 stores of ...
Giant Spontaneous Depolarizing Potentials in the Developing
Giant Spontaneous Depolarizing Potentials in the Developing

... spontaneously at frequencies of 0.02– 0.06 Hz and was completely abolished by GABAAR antagonist SR 95531 (10 ␮M; Fig. 5C), thus we termed these events thalamic giant GABAergic spontaneous depolarizing potentials (tGDPs). In the following analysis, when we use the term tGDP, we are referring to the e ...
Text - Enlighten: Publications
Text - Enlighten: Publications

... Figure 2 Live cell LM15 labeling of wild type (wt) Arabidopsis root hairs. A: Overview of live root demonstrating strong LM15 labeling of root hairs (arrows) and epidermis (hollow arrow). Bar = 100 μm. B: Magnified view of a root hair labeled with LM15 (arrow). Bar = 19 μm. C and D: LM15 labeling of ...
The O-antigen mediates differential survival of
The O-antigen mediates differential survival of

... from other bacterial cells by GFP-fluorescence and resistance to chloramphenicol, gentamicin and zeomycin. Prior to strain addition, sediment was either left untreated, treated with heat or treated with a cocktail of three antibiotics targeting eukaryotic predators. Samples were recovered over time ...
Chara Myosin and the Energy of Cytoplasmic Streaming
Chara Myosin and the Energy of Cytoplasmic Streaming

... fluorescent spots on actin cables probably represent artifactual myosin stack due to fixation. Glutaraldehyde cannot stop all myosin molecules at once. If one myosin molecule stops moving due to the glutaraldehyde fixation, it will disturb other myosin molecules following the same actin track. This ...
Ruditapes decussatus (Mollusca: Bivalvia)
Ruditapes decussatus (Mollusca: Bivalvia)

... the phagocytic process can be subdivided into 4 steps: (1) chemotaxis, involving the attraction of the phagocyte to the non-self material, (2) recognition, involving the attachment of the foreign material to the surface of the phagocyte, (3) internalization, and (4) intracellular degradation. Light ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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