Basic Fibroblast Growth Factor in Atria and Ventricles of the
... weight) resulted in ~1.5 vol of extract with a protein concentration of 15-17 mg/ml. MEM was used so that extracts could be tested immediately for biological activity on cells in vitro. Representative results are shown in Fig. 1, where extracts have been tested at a final concentration of 0.5 mg/ml. ...
... weight) resulted in ~1.5 vol of extract with a protein concentration of 15-17 mg/ml. MEM was used so that extracts could be tested immediately for biological activity on cells in vitro. Representative results are shown in Fig. 1, where extracts have been tested at a final concentration of 0.5 mg/ml. ...
Cell death by autophagy: facts and apparent artefacts
... in vitro experiments do not take into account any contextdependent, in vivo (as in tumours) and developmental role of autophagy in cell death. While cell death by autophagy, to our current knowledge, is relatively uncommon, studies using specific model organisms and systems have provided convincing ...
... in vitro experiments do not take into account any contextdependent, in vivo (as in tumours) and developmental role of autophagy in cell death. While cell death by autophagy, to our current knowledge, is relatively uncommon, studies using specific model organisms and systems have provided convincing ...
Unit 6 Microorganisms & Fungi
... Amoeboid movement - when the cytoplasm of the cell streams into the pseudopod, & the rest of the cell follows Food vacuole - a small cavity in the cytoplasm that temporarily stores food ...
... Amoeboid movement - when the cytoplasm of the cell streams into the pseudopod, & the rest of the cell follows Food vacuole - a small cavity in the cytoplasm that temporarily stores food ...
Daughter-Specific Transcription Factors Regulate Cell Size Control
... In budding yeast, asymmetric cell division yields a larger mother and a smaller daughter cell, which transcribe different genes due to the daughter-specific transcription factors Ace2 and Ash1. Cell size control at the Start checkpoint has long been considered to be a main regulator of the length of ...
... In budding yeast, asymmetric cell division yields a larger mother and a smaller daughter cell, which transcribe different genes due to the daughter-specific transcription factors Ace2 and Ash1. Cell size control at the Start checkpoint has long been considered to be a main regulator of the length of ...
Inside A Cell
... 1. I feel like jelly, but my job is important. I am the place where molecules are broken down to produce energy and where other molecules are built up. What am I? ____________________ 2. I am a thin layer that surrounds the cell. I hold all of the organelles in place. I let some things into the cell ...
... 1. I feel like jelly, but my job is important. I am the place where molecules are broken down to produce energy and where other molecules are built up. What am I? ____________________ 2. I am a thin layer that surrounds the cell. I hold all of the organelles in place. I let some things into the cell ...
Profile of Resistance of Human Immunodeficiency Virus to Mannose
... nanogram per milliliter range and suppress syncytium formation between persistently HIV type 1 (HIV-1)infected cells and uninfected CD4ⴙ T cells. These lectins inhibit virus entry. When exposed to escalating concentrations of G. nivalis and Hippeastrum sp. hybrid agglutinin, a variety of HIV-1(IIIB) ...
... nanogram per milliliter range and suppress syncytium formation between persistently HIV type 1 (HIV-1)infected cells and uninfected CD4ⴙ T cells. These lectins inhibit virus entry. When exposed to escalating concentrations of G. nivalis and Hippeastrum sp. hybrid agglutinin, a variety of HIV-1(IIIB) ...
The Arabidopsis Rab GTPase family: another
... least 60 different Rab proteins that are ascribed to about 40 different functional subclasses [7,11••,12•]. In the Arabidopsis genome sequence, we and others [11••] have identified 57 loci that can encode Rab GTPases. 48 of these are known as cDNAs or expressed sequence tags (ESTs) or can be amplifi ...
... least 60 different Rab proteins that are ascribed to about 40 different functional subclasses [7,11••,12•]. In the Arabidopsis genome sequence, we and others [11••] have identified 57 loci that can encode Rab GTPases. 48 of these are known as cDNAs or expressed sequence tags (ESTs) or can be amplifi ...
Pancreas or liver choice within endoderm
... Temporal and spatial relation between pancreas and liver specification We first sought to define the exact time and place where the earliest markers of the pancreas and liver are activated. RT-PCR was used to investigate Pdx1 gene expression in endoderm fragments dissected from the ventral foregut o ...
... Temporal and spatial relation between pancreas and liver specification We first sought to define the exact time and place where the earliest markers of the pancreas and liver are activated. RT-PCR was used to investigate Pdx1 gene expression in endoderm fragments dissected from the ventral foregut o ...
casanova and endoderm formation - Development
... cas mutants do not express any known endodermal marker during gastrulation and do not differentiate endodermal derivatives (Alexander et al., 1999; Alexander and Stainier, 1999). Activation of Nodal signalling by ectopic expression of tar*, bon/mixer or fau/gata5 fail to induce endoderm in cas mutan ...
... cas mutants do not express any known endodermal marker during gastrulation and do not differentiate endodermal derivatives (Alexander et al., 1999; Alexander and Stainier, 1999). Activation of Nodal signalling by ectopic expression of tar*, bon/mixer or fau/gata5 fail to induce endoderm in cas mutan ...
Aquatic Microbial Ecology 58:303
... This pattern might be due to this particular ecosystem, which is shallow, with high productivity and activity of coral, and is affected by tidal cycle, light intensity, temperature, nutrients, and other factors. Of the environmental factors we measured, variations in tidal height were most similar t ...
... This pattern might be due to this particular ecosystem, which is shallow, with high productivity and activity of coral, and is affected by tidal cycle, light intensity, temperature, nutrients, and other factors. Of the environmental factors we measured, variations in tidal height were most similar t ...
Balancing mitochondrial biogenesis and
... remained elusive. We found that age-related mitophagy decline causes progressive accumulation of mitochondria in the nematode Caenorhabditis elegans. Inhibition of either general autophagy or specifically mitophagy increases mitochondrial mass, recapitulating the effects of ageing in young individua ...
... remained elusive. We found that age-related mitophagy decline causes progressive accumulation of mitochondria in the nematode Caenorhabditis elegans. Inhibition of either general autophagy or specifically mitophagy increases mitochondrial mass, recapitulating the effects of ageing in young individua ...
Cytokinin Lecture
... (A) Wild type (B) Plants over-expressing cytokinin oxidase to reduce the level of cytokinin. Root meristem is indicated in bright blue (nuclear DNA staining). ...
... (A) Wild type (B) Plants over-expressing cytokinin oxidase to reduce the level of cytokinin. Root meristem is indicated in bright blue (nuclear DNA staining). ...
Mapping the function of the NS3 protein of African horsesickness
... function in the viral life cycle is not yet fully understood. The non-structural protein NS3 is believed to be mediate virus release from infected cells. Although it has generally been accepted that non-enveloped viruses are released from infected cells after cellular lysis, available data for Bluet ...
... function in the viral life cycle is not yet fully understood. The non-structural protein NS3 is believed to be mediate virus release from infected cells. Although it has generally been accepted that non-enveloped viruses are released from infected cells after cellular lysis, available data for Bluet ...
Original Article Intracellular zinc distribution in mitochondria, ER and
... regulating many physiological processes. It is involved in DNA and protein synthesis, hormone packaging, mitosis, apoptosis, and many other functions [1]. Zinc is found within the cells in both a protein-bound state and as a free or chelatable divalent ion. In the protein-bound form, zinc, as a stru ...
... regulating many physiological processes. It is involved in DNA and protein synthesis, hormone packaging, mitosis, apoptosis, and many other functions [1]. Zinc is found within the cells in both a protein-bound state and as a free or chelatable divalent ion. In the protein-bound form, zinc, as a stru ...
Part 1 - ISpatula
... “The lower is the CMC value of a given amphiphilic polymer, the more stable are micelles even at low concentration of an amphiphile in the medium. This is especially important from the pharmacological point of view, since upon the dilution with the large volume of the blood only micelles with low CM ...
... “The lower is the CMC value of a given amphiphilic polymer, the more stable are micelles even at low concentration of an amphiphile in the medium. This is especially important from the pharmacological point of view, since upon the dilution with the large volume of the blood only micelles with low CM ...
physiology of ion transport across the tonoplast of higher plants
... endomembrane distribution, or in acidification of a localized extracellular compartment in the case of the plasma membrane V-ATPase (84). The enzyme resembles the F0F1-ATPases (F-ATPases) of mitochondria, chloroplasts, and eubacteria in their multimeric structure, lack of a phosphorylated intermedia ...
... endomembrane distribution, or in acidification of a localized extracellular compartment in the case of the plasma membrane V-ATPase (84). The enzyme resembles the F0F1-ATPases (F-ATPases) of mitochondria, chloroplasts, and eubacteria in their multimeric structure, lack of a phosphorylated intermedia ...
Subcellular trafficking kinetics of GLUT4 mutated at the N
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
VP5 autocleavage is required for efficient infection by in vitro
... infectious GCRV particles have demonstrated that complete digestion of VP7 and partial cleavage of VP5 can lead to enhanced infectivity of the virus, indicating that VP7 and VP5 may play important roles in GCRV entry into host cells during infection (Fang et al., 2008; Zhang et al., 2010). Recent th ...
... infectious GCRV particles have demonstrated that complete digestion of VP7 and partial cleavage of VP5 can lead to enhanced infectivity of the virus, indicating that VP7 and VP5 may play important roles in GCRV entry into host cells during infection (Fang et al., 2008; Zhang et al., 2010). Recent th ...
pdf file - John Innes Centre
... One of the most important aspects of this type of endosymbiotic association is the identification of the molecular mechanism by which bacteroids are able to fix nitrogen but, unlike free-living diazotrophs, are unable to assimilate the NH3 produced. In fact, although it was suggested some time ago t ...
... One of the most important aspects of this type of endosymbiotic association is the identification of the molecular mechanism by which bacteroids are able to fix nitrogen but, unlike free-living diazotrophs, are unable to assimilate the NH3 produced. In fact, although it was suggested some time ago t ...
Merlin and Expanded interact - Development
... The regulation of cell proliferation is central to controlling the size and shape of multicellular organisms. Disruption of proliferation control in an organism can have severe consequences; improper activation of cell proliferation can result in the formation of tumors and the development of cancer ...
... The regulation of cell proliferation is central to controlling the size and shape of multicellular organisms. Disruption of proliferation control in an organism can have severe consequences; improper activation of cell proliferation can result in the formation of tumors and the development of cancer ...
Mechanisms associated with phagocytosis of
... water. Arcobacter butzleri is an emerging potential zoonotic pathogen that can be isolated from environmental water sources, where they can establish endosymbiotic relationships with amoebas. The aim of this study was to describe the implication of mannose-binding proteins and membraneassociated rec ...
... water. Arcobacter butzleri is an emerging potential zoonotic pathogen that can be isolated from environmental water sources, where they can establish endosymbiotic relationships with amoebas. The aim of this study was to describe the implication of mannose-binding proteins and membraneassociated rec ...
Structure-Function Relationships of Stem Cell Factor
... murine SCF were subjected to site-directed mutagenesis using a polymerase chain reaction (PCR)-based technique3' to introduce useful restriction sites for subcloning and to generate interspecies chimeric cDNA. The resultant cDNA were subcloned into the mammalian cell expression vector pD540and the n ...
... murine SCF were subjected to site-directed mutagenesis using a polymerase chain reaction (PCR)-based technique3' to introduce useful restriction sites for subcloning and to generate interspecies chimeric cDNA. The resultant cDNA were subcloned into the mammalian cell expression vector pD540and the n ...
Long-Circulating and Target-Specific Nanoparticles: Theory to Practice
... not via targeting but through macrophage loading (Seltzer, 1989; Tilcock, 1995; Kostarelos and Emfietzoglou, 1999). By this approach, it is the surrounding parenchyma, and not the pathology, that will change in intensity. The rapid sequestration of intravenously injected colloidal particles from the ...
... not via targeting but through macrophage loading (Seltzer, 1989; Tilcock, 1995; Kostarelos and Emfietzoglou, 1999). By this approach, it is the surrounding parenchyma, and not the pathology, that will change in intensity. The rapid sequestration of intravenously injected colloidal particles from the ...