Cell adhesion and cell mechanics during zebrafish
... and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression among the emergent cell types [2]. At the end, however, it is not sufficient t ...
... and build complex structures such as eyes, heart or brain (see Fig. 1.1). But how can cells that originate from a single ancestor control all these processes? Surely, an important aspect are differences in gene expression among the emergent cell types [2]. At the end, however, it is not sufficient t ...
Re-establishing the avian body plan - Development
... LBIs were used. These were constructed at stage 3a/b and the two LBIs obtained from each blastoderm were left interconnected cranially (Fig. 1C). The blastoderm 250 µm adjacent to the primitive streak was removed on the ‘left’ side, but it was kept undisturbed on the ‘right’ side. Because the adjace ...
... LBIs were used. These were constructed at stage 3a/b and the two LBIs obtained from each blastoderm were left interconnected cranially (Fig. 1C). The blastoderm 250 µm adjacent to the primitive streak was removed on the ‘left’ side, but it was kept undisturbed on the ‘right’ side. Because the adjace ...
Leaf development - The Virtual Plant
... 2. Guaranteeing a supply of cells -the vascular cambium The shoot apical meristem has an intrinsic capacity for self regulation and the positional specification of its cells implies the existence of an elaborate and versatile communication network. Van der Schoot and Rinne2 proposed a model that pi ...
... 2. Guaranteeing a supply of cells -the vascular cambium The shoot apical meristem has an intrinsic capacity for self regulation and the positional specification of its cells implies the existence of an elaborate and versatile communication network. Van der Schoot and Rinne2 proposed a model that pi ...
Neutrophils injure cultured skeletal myotubes
... Electron microscopy. Transmission and scanning electron microscopy were performed on several control cultures (myotubes only) and on cultures containing both neutrophils (nonin vitro-stimulated) and myotubes (E:T ratio of 5). Because of the severity of the neutrophil-mediated myotube injury at E:T r ...
... Electron microscopy. Transmission and scanning electron microscopy were performed on several control cultures (myotubes only) and on cultures containing both neutrophils (nonin vitro-stimulated) and myotubes (E:T ratio of 5). Because of the severity of the neutrophil-mediated myotube injury at E:T r ...
Optimal Staining and Sample Storage Time for Direct Microscopic
... enumeration, especially in instances of high background fluorescence (14). DAPI has also been recommended over another DNA-specific dye, mithramycin (4). Several methods have been established for enumeration of active bacteria. The INT [2-(p-iodophenyl)-3-(p-nitrophenyl)5-phenyl tetrazolium chloride ...
... enumeration, especially in instances of high background fluorescence (14). DAPI has also been recommended over another DNA-specific dye, mithramycin (4). Several methods have been established for enumeration of active bacteria. The INT [2-(p-iodophenyl)-3-(p-nitrophenyl)5-phenyl tetrazolium chloride ...
Cryptic O2 –-generating NADPH oxidase in
... Culture of dendritic cells from splenic progenitors DCs derived from BALB/c murine splenic progenitors were grown in an Iscove DMEM medium supplemented with 10% fetal calf serum (FCS), GM-CSF and Flt-3 ligand (Flt-3L) as previously described (Berthier et al., 2000; Martinon-Ego et al., 2001). On day ...
... Culture of dendritic cells from splenic progenitors DCs derived from BALB/c murine splenic progenitors were grown in an Iscove DMEM medium supplemented with 10% fetal calf serum (FCS), GM-CSF and Flt-3 ligand (Flt-3L) as previously described (Berthier et al., 2000; Martinon-Ego et al., 2001). On day ...
Differential expression of genes under control of the
... were divided into several compartments by simple septa. Since formation of clamp connections is closely associated with the dikaryotic state of the M A T A + M A T B + heterokaryon, we attempted to analyse the nuclear distribution. However, we were unable to stain nuclei in fullgrown aerial hyphae. ...
... were divided into several compartments by simple septa. Since formation of clamp connections is closely associated with the dikaryotic state of the M A T A + M A T B + heterokaryon, we attempted to analyse the nuclear distribution. However, we were unable to stain nuclei in fullgrown aerial hyphae. ...
pdf: Rahman et al. 2007.
... (Stasinopoulos and Hangarter, 1990). To characterize the mechanism of inhibition of root elongation by auxin, we pursued a kinematic approach. First, we evaluated the time course of root elongation rate (Figure 1a–c). Seedlings were grown for 4 days, transplanted onto medium with auxins as indicated ...
... (Stasinopoulos and Hangarter, 1990). To characterize the mechanism of inhibition of root elongation by auxin, we pursued a kinematic approach. First, we evaluated the time course of root elongation rate (Figure 1a–c). Seedlings were grown for 4 days, transplanted onto medium with auxins as indicated ...
ACEA Biosciences and Hurel Corporation Announce Joint
... Hurel’s cell-based products, tradenamed HμRELhuman™, HμRELdog™, HμRELprimate™, and HμRELrat™, are cellular co-cultures made from actual liver cells derived from the human, canine, cynomolgus monkey, or rat species, respectively. Hurel’s products are distinctive for their high levels of metabolic com ...
... Hurel’s cell-based products, tradenamed HμRELhuman™, HμRELdog™, HμRELprimate™, and HμRELrat™, are cellular co-cultures made from actual liver cells derived from the human, canine, cynomolgus monkey, or rat species, respectively. Hurel’s products are distinctive for their high levels of metabolic com ...
Catecholamines Stimulate Testicular Steroidogenesis In Vitro in the
... provide a more physiological method than Leydig cell culture. The presence of other testicular cells may well be the reason for the observed effects of catecholamines on the production of testosterone by the testes of the golden and Siberian hamsters. These effects of catecholamines could be due to ...
... provide a more physiological method than Leydig cell culture. The presence of other testicular cells may well be the reason for the observed effects of catecholamines on the production of testosterone by the testes of the golden and Siberian hamsters. These effects of catecholamines could be due to ...
Title Molecular Pathways: The Role of Primary Cilia in Cancer
... are expected to be effective as anti-cancer drugs through killing cancer cells as well as through targeting stromal cells associated with tumorigenesis (e.g. inhibition of angiogenesis). In this section, our focus is on the role cilia play in the efficacy of Hhtargeted drugs specifically on cancer c ...
... are expected to be effective as anti-cancer drugs through killing cancer cells as well as through targeting stromal cells associated with tumorigenesis (e.g. inhibition of angiogenesis). In this section, our focus is on the role cilia play in the efficacy of Hhtargeted drugs specifically on cancer c ...
Midgut and muscle development in Drosophila melanogaster
... thousands of researchers around the world used this model organism to study rules of development from a simple fertilized egg into an adult. This process of development, which is similar to the one taking place in us, has made the fruitfly a popular model to study complex biological processes and an ...
... thousands of researchers around the world used this model organism to study rules of development from a simple fertilized egg into an adult. This process of development, which is similar to the one taking place in us, has made the fruitfly a popular model to study complex biological processes and an ...
Distinct Lytic Vacuolar Compartments are Embedded
... germination, storage proteins, which provide a source of reduced nitrogen, and inorganic minerals need to be mobilized to support seedling growth. In addition, a lytic aqueous vacuolar compartment has to be formed to provide turgor pressure driving cell expansion and to promote radicle protrusion an ...
... germination, storage proteins, which provide a source of reduced nitrogen, and inorganic minerals need to be mobilized to support seedling growth. In addition, a lytic aqueous vacuolar compartment has to be formed to provide turgor pressure driving cell expansion and to promote radicle protrusion an ...
Uncoupling effect of polyunsaturated fatty acid deficiency in isolated
... method of Berry and Friend [10] as modified by Groen et al. [11]. At the time of killing, for the same age, the body masses in the control group were slightly but significantly higher than those of the PUFA-deficient group (270³8 g compared with 234³7 g ; results given ³S.E.M. ; n ¯ 12 in each group ...
... method of Berry and Friend [10] as modified by Groen et al. [11]. At the time of killing, for the same age, the body masses in the control group were slightly but significantly higher than those of the PUFA-deficient group (270³8 g compared with 234³7 g ; results given ³S.E.M. ; n ¯ 12 in each group ...
high-throughput transient gene expression in plant
... or polyethylene glycol (PEG)-mediated transfection with foreign materials such as plasmid DNA, RNA or viruses (Dixon, 1994; Koop et aI., 1996). It has been shown that when protoplasts from a variety of plant systems were inoculated in vitro with TMV, virus infected and multiplied without causing nec ...
... or polyethylene glycol (PEG)-mediated transfection with foreign materials such as plasmid DNA, RNA or viruses (Dixon, 1994; Koop et aI., 1996). It has been shown that when protoplasts from a variety of plant systems were inoculated in vitro with TMV, virus infected and multiplied without causing nec ...
Molecular characterization of human and mouse fatty acid amide
... serving to terminate the signaling functions of these molecules. Here, we report the molecular characterization of both a mouse and a human FAAH and compare these enzymes to the rat FAAH. The enzymes are well conserved in primary structure, with the mouse and rat FAAHs sharing 91% amino acid identit ...
... serving to terminate the signaling functions of these molecules. Here, we report the molecular characterization of both a mouse and a human FAAH and compare these enzymes to the rat FAAH. The enzymes are well conserved in primary structure, with the mouse and rat FAAHs sharing 91% amino acid identit ...
CDK2 regulates nuclear envelope protein dynamics and telomere
... then explored whether the structure of the nuclear lamina was similarly impaired. To verify this, we performed doubleimmunolabeling experiments of SYCP3 with LAP2, another INM integral protein, or lamin C2, a meiosis-specific isoform of lamin A that appears at the docking sites for meiotic telomeres ...
... then explored whether the structure of the nuclear lamina was similarly impaired. To verify this, we performed doubleimmunolabeling experiments of SYCP3 with LAP2, another INM integral protein, or lamin C2, a meiosis-specific isoform of lamin A that appears at the docking sites for meiotic telomeres ...
The Development of Radial and Biradial Symmetry: The Evolution of
... by a largely acellular mesoglea. The cnidarians generally have multiple planes of mirror symmetry which pass through the major longitudinal axis, the oral-aboral axis. A. Cross section of the benthic polyp form which attaches to the substrate via the basal discs located at the aboral pole. Numerous ...
... by a largely acellular mesoglea. The cnidarians generally have multiple planes of mirror symmetry which pass through the major longitudinal axis, the oral-aboral axis. A. Cross section of the benthic polyp form which attaches to the substrate via the basal discs located at the aboral pole. Numerous ...
Female meiosis in polo - Journal of Cell Science
... 1998). Finally, the polo like kinases have been implicated in the late mitotic pathway that establishes conditions for correct cytokinesis. Two phenotypes predominate in disruptants of the fission yeast Plk gene plo1, namely monopolar mitotic spindles resulting from a failure of spindle pole bodies ...
... 1998). Finally, the polo like kinases have been implicated in the late mitotic pathway that establishes conditions for correct cytokinesis. Two phenotypes predominate in disruptants of the fission yeast Plk gene plo1, namely monopolar mitotic spindles resulting from a failure of spindle pole bodies ...
Arachidonic Acid and Docosahexaenoic Acid Suppress Osteoclast Formation and Activity
... between the osteoblasts and osteoclasts is crucial in maintaining the structure of the bone tissue. Osteoclasts, the sole bone resorbing cell in the body, are multinuclear, terminally differentiated cells that are derived from haematopoietic precursors of the monocyte/macrophage lineage [3, 4, 5, 6] ...
... between the osteoblasts and osteoclasts is crucial in maintaining the structure of the bone tissue. Osteoclasts, the sole bone resorbing cell in the body, are multinuclear, terminally differentiated cells that are derived from haematopoietic precursors of the monocyte/macrophage lineage [3, 4, 5, 6] ...
Physiological assembly and activity of human
... and cancer (Vulliamy et al., 2006). These diverse clinical presentations are just an inkling of the much greater phenotypic diversity that may be linked to telomere maintenance deficiency in the future. The spectrum of phenotypes can be much broader than currently anticipated, because mutations that ...
... and cancer (Vulliamy et al., 2006). These diverse clinical presentations are just an inkling of the much greater phenotypic diversity that may be linked to telomere maintenance deficiency in the future. The spectrum of phenotypes can be much broader than currently anticipated, because mutations that ...
Enhancement of Murine Lymphokine-activated Killer Cell Activity by
... culture. However, cellular serine protease activity, measured as N-abenzyloxycarbonyl-i.-lysine thiobenzyl-esterase, in LAK cells was in creased by retinoic acid also in a dose- and time-dependent manner. The increase in LAK cellular serine protease activity was significantly corre lated with that o ...
... culture. However, cellular serine protease activity, measured as N-abenzyloxycarbonyl-i.-lysine thiobenzyl-esterase, in LAK cells was in creased by retinoic acid also in a dose- and time-dependent manner. The increase in LAK cellular serine protease activity was significantly corre lated with that o ...
Differential localization of LTA synthesis proteins and their
... 2009; Webb et al., 2009; Wörmann et al., 2011a). This is further emphasized by the observation that the B. subtilis glycosyltransferase UgtP, the homologue of the S. aureus YpfP protein, as well as LtaS and YqgS, two of the four LtaS homologues, localize to the cell division site in this organism (N ...
... 2009; Webb et al., 2009; Wörmann et al., 2011a). This is further emphasized by the observation that the B. subtilis glycosyltransferase UgtP, the homologue of the S. aureus YpfP protein, as well as LtaS and YqgS, two of the four LtaS homologues, localize to the cell division site in this organism (N ...
eXtra Botany - Oxford Academic
... Boot et al. (2012) suggest that an auxin influx carrier may not be involved in PAT in the characeans, based on observations of auxin efflux. While all tissues in which PAT has been characterized (embryophyte sporophytes; Chara) or may occur (some complex brown algae: Sun et al., 2004; Le Bail et al. ...
... Boot et al. (2012) suggest that an auxin influx carrier may not be involved in PAT in the characeans, based on observations of auxin efflux. While all tissues in which PAT has been characterized (embryophyte sporophytes; Chara) or may occur (some complex brown algae: Sun et al., 2004; Le Bail et al. ...