05 October 2000
... chacoense Bitt. lines were obtained from the Potato Introduction Station (Sturgeon Bay, Wisconsin). The self-incompatibility alleles in the parental line PI458314 were identified by genetic crosses and gene sequence as S11 and S12 (ref. 20 ) and the alleles in the parental line PI230582 identified a ...
... chacoense Bitt. lines were obtained from the Potato Introduction Station (Sturgeon Bay, Wisconsin). The self-incompatibility alleles in the parental line PI458314 were identified by genetic crosses and gene sequence as S11 and S12 (ref. 20 ) and the alleles in the parental line PI230582 identified a ...
Actin and Myosin Function in Directed Vacuole Movement during
... systems and it appears that the mechanisms involved vary among different organisms, as well as for different organelles within the same cell. During mitosis in mammalian cells, the ER and Golgi apparatus become fragmented, forming many small vesicles that are specifically ...
... systems and it appears that the mechanisms involved vary among different organisms, as well as for different organelles within the same cell. During mitosis in mammalian cells, the ER and Golgi apparatus become fragmented, forming many small vesicles that are specifically ...
Molecular organization of the cell wall of Candida albicans and its
... Synthesis of chitin involves a transglycosylation reaction of GlcNAc residues from the universal substrate UDP-Nacetylglucosamine to the growing chain of the polysaccharide. The reaction (catalyzed by ill-known enzymes called chitin synthases, Chsps) requires a divalent metal, generally Mg21, but do ...
... Synthesis of chitin involves a transglycosylation reaction of GlcNAc residues from the universal substrate UDP-Nacetylglucosamine to the growing chain of the polysaccharide. The reaction (catalyzed by ill-known enzymes called chitin synthases, Chsps) requires a divalent metal, generally Mg21, but do ...
Electrical Properties of the Pacemaker Neurons in the Heart
... also be observed with the light microscope (Fig. 2). The axons approach each other, become enclosed within a common sheath, and lose their round shape. Two or three (sometimes four) axons could be involved. The intervening sheaths become gradually thinner and finally are almost impossible to detect ...
... also be observed with the light microscope (Fig. 2). The axons approach each other, become enclosed within a common sheath, and lose their round shape. Two or three (sometimes four) axons could be involved. The intervening sheaths become gradually thinner and finally are almost impossible to detect ...
Review THE SELECTION OF APTAMERS SPECIFIC FOR
... - The preparation of a modified ssDNA aptamer containing photo-active 5-iodo-deoxyuridine within the sequence (the first four and final four nucleotide regions), and a biotin at the 3’ end and a fluorescence probe at the 5’ end. The binding of the modified aptamer to the Ramos cells was confirmed us ...
... - The preparation of a modified ssDNA aptamer containing photo-active 5-iodo-deoxyuridine within the sequence (the first four and final four nucleotide regions), and a biotin at the 3’ end and a fluorescence probe at the 5’ end. The binding of the modified aptamer to the Ramos cells was confirmed us ...
Nociceptor and Hair Cell Transducer Properties of TRPA1, a
... these small neurons are polymodal and detect more than one type of noxious stimulus. TRPA1 (also known as ANKTM1 and P120) (Jaquemar et al., 1999) is a member of branch A of the transient receptor potential (TRP) family of cation channels. It has been reported that TRPA1 is expressed in a small numb ...
... these small neurons are polymodal and detect more than one type of noxious stimulus. TRPA1 (also known as ANKTM1 and P120) (Jaquemar et al., 1999) is a member of branch A of the transient receptor potential (TRP) family of cation channels. It has been reported that TRPA1 is expressed in a small numb ...
Arachidonic Acid Mediates Interleukin-1 and Tumor
... pmoI/L arachidonic acid, respectively (Fig IB). Further increases in the concentration of arachidonic acid did not further potentiate JNK activity (data not shown). The effect of arachidonic acid did not appear to be a result of generalized cytotoxicity, because LDH was not detectable in supematants ...
... pmoI/L arachidonic acid, respectively (Fig IB). Further increases in the concentration of arachidonic acid did not further potentiate JNK activity (data not shown). The effect of arachidonic acid did not appear to be a result of generalized cytotoxicity, because LDH was not detectable in supematants ...
BRAIN CCR2 Ly-6C monocytes are crucial for the
... Since the initial description in the late 1990s of the immunological phenotype of mice lacking the chemokine receptor CCR2 (Boring et al., 1997; Kuziel et al., 1997), there have been numerous publications examining the effects of CCR2 deficiency in a variety of murine models of infectious and autoim ...
... Since the initial description in the late 1990s of the immunological phenotype of mice lacking the chemokine receptor CCR2 (Boring et al., 1997; Kuziel et al., 1997), there have been numerous publications examining the effects of CCR2 deficiency in a variety of murine models of infectious and autoim ...
Mucolipin 1 channel activity is regulated by protein kinase A
... knockout of cup-5, the orthologue of MCOLN1 in Caenorhabditis elegans, resulted in formation of enlarged hybrid organelles that contained both late-endosomal and lysosomal markers [21]. Recently, several groups have suggested additional roles for MCOLN1 in different cellular processes, including reg ...
... knockout of cup-5, the orthologue of MCOLN1 in Caenorhabditis elegans, resulted in formation of enlarged hybrid organelles that contained both late-endosomal and lysosomal markers [21]. Recently, several groups have suggested additional roles for MCOLN1 in different cellular processes, including reg ...
The PadR-like transcriptional regulator LftR ensures efficient
... Published: 28 July 2015 Citation: Kaval KG, Hahn B, Tusamda N, Albrecht D and Halbedel S (2015) The PadR-like transcriptional regulator LftR ensures efficient invasion of Listeria monocytogenes into human host cells. Front. Microbiol. 6:772. doi: 10.3389/fmicb.2015.00772 ...
... Published: 28 July 2015 Citation: Kaval KG, Hahn B, Tusamda N, Albrecht D and Halbedel S (2015) The PadR-like transcriptional regulator LftR ensures efficient invasion of Listeria monocytogenes into human host cells. Front. Microbiol. 6:772. doi: 10.3389/fmicb.2015.00772 ...
- Wiley Online Library
... (0.5 lg ml)1). Cells were examined by fluorescence microscopy 15 min after FITC-EIX application (Figure 5). As shown in Figure 5, treatment with the endocytosis inhibitors did not affect the ability of EIX to bind tobacco cells, but destroyed the ability of the cell to internalize LeEix2 in the case ...
... (0.5 lg ml)1). Cells were examined by fluorescence microscopy 15 min after FITC-EIX application (Figure 5). As shown in Figure 5, treatment with the endocytosis inhibitors did not affect the ability of EIX to bind tobacco cells, but destroyed the ability of the cell to internalize LeEix2 in the case ...
Synaptic Potentials Mediated via
... application of nicotine also was used to determine whether the responses were mediated via nAChRs. In four cells, superfusion with 1 mM nicotine reversibly reduced the amplitude of the MLAsensitive EPSC by 74 6 7.3% (see Fig. 2 D,E) without producing detectable changes in the holding current (Fig. 3 ...
... application of nicotine also was used to determine whether the responses were mediated via nAChRs. In four cells, superfusion with 1 mM nicotine reversibly reduced the amplitude of the MLAsensitive EPSC by 74 6 7.3% (see Fig. 2 D,E) without producing detectable changes in the holding current (Fig. 3 ...
Perspectives in active liquid crystals
... case of swimming bacteria and active colloids, or a solid, as for cells crawling on a substrate. In some cases, the medium can simply be described as a passive momentum sink, providing friction (see, for instance, [19]). This class of systems, referred to as ‘dry’, includes cells crawling on a rigid ...
... case of swimming bacteria and active colloids, or a solid, as for cells crawling on a substrate. In some cases, the medium can simply be described as a passive momentum sink, providing friction (see, for instance, [19]). This class of systems, referred to as ‘dry’, includes cells crawling on a rigid ...
Silicification in the Microalgae
... are known to form internal biomineralized siliceous elements. Within Actiniscus pentastarias var. arcticus, 1–8 large and up to 14 smaller rudimentary star-shaped siliceous structures (pentasters) have been observed (Fig. 1e), although twin perinuclear penasters that surround the nucleus are most co ...
... are known to form internal biomineralized siliceous elements. Within Actiniscus pentastarias var. arcticus, 1–8 large and up to 14 smaller rudimentary star-shaped siliceous structures (pentasters) have been observed (Fig. 1e), although twin perinuclear penasters that surround the nucleus are most co ...
Osmotic Effects on the Electrical Properties of
... * Corresponding author; e-mail [email protected]; fax ...
... * Corresponding author; e-mail [email protected]; fax ...
PDF hosted at the Radboud Repository of the Radboud University Nijmegen
... T cell-mediated immunity, Ag has to be presented to naïve T cells as a processed peptide in the context of a major histocompatibility complex (MHC) molecule by specialized antigen-presenting cells (APCs). The most important APC is the dendritic cell (DC, see below), but also B cells and macrophages ...
... T cell-mediated immunity, Ag has to be presented to naïve T cells as a processed peptide in the context of a major histocompatibility complex (MHC) molecule by specialized antigen-presenting cells (APCs). The most important APC is the dendritic cell (DC, see below), but also B cells and macrophages ...
SYNTHETIC PEPTIDES AS ANTIGENS AND COMPETITORS IN
... synthetic peptides corresponding to residues 171-186 or 171-182 of HLACW3 (14-16) . In the present study, we show that antigens recognized by Kd -restricted CTL specific for HLA-A24 can also be mimicked by synthetic peptides, and that several distinct CW3 and A24 epitopes map to the COOH-terminal en ...
... synthetic peptides corresponding to residues 171-186 or 171-182 of HLACW3 (14-16) . In the present study, we show that antigens recognized by Kd -restricted CTL specific for HLA-A24 can also be mimicked by synthetic peptides, and that several distinct CW3 and A24 epitopes map to the COOH-terminal en ...
Microtubule Independent Vesiculation of Golgi Membranes and the
... with nocodazole (18-24 h) arrests cells in mitosis by inhibiting the dynamics of the mitotic spindle (Jordan et al., 1992). The Golgi membranes under these conditions are found to be composed of small vesicular structures and are thought to represent the mitotic form of Golgi (Lucocq et al., 1987a,b ...
... with nocodazole (18-24 h) arrests cells in mitosis by inhibiting the dynamics of the mitotic spindle (Jordan et al., 1992). The Golgi membranes under these conditions are found to be composed of small vesicular structures and are thought to represent the mitotic form of Golgi (Lucocq et al., 1987a,b ...
μMACS™ mRNA Isolation Kits
... core components are superparamagnetic Oligo(dT) MicroBeads that hybridize to the stretch of adenosine residues at the 3´end of eukaryotic mRNA. The magnetically labeled mRNA can then be isolated on μ Columns or M Columns placed in a MACS Separator. After magnetic isolation, the mRNA can be eluted fr ...
... core components are superparamagnetic Oligo(dT) MicroBeads that hybridize to the stretch of adenosine residues at the 3´end of eukaryotic mRNA. The magnetically labeled mRNA can then be isolated on μ Columns or M Columns placed in a MACS Separator. After magnetic isolation, the mRNA can be eluted fr ...
1 DNA replication origin function is promoted by H3K4 di
... surrounding origins plays an essential role in controlling origin activity. For instance, the positioning of nucleosomes near origins can either stimulate or inhibit origin function (Simpson 1990; Crampton et al. 2008; Berbenetz et al. 2010; Eaton et al. 2010). The major protein components of chrom ...
... surrounding origins plays an essential role in controlling origin activity. For instance, the positioning of nucleosomes near origins can either stimulate or inhibit origin function (Simpson 1990; Crampton et al. 2008; Berbenetz et al. 2010; Eaton et al. 2010). The major protein components of chrom ...
Physiology of intracellular potassium channels: A unifying role as
... relevant because the pool of small anions in the mitochondrial matrix is limited compared with the cytosolic K+ pool. To compensate for charge movement, the RC must increase the rate of proton transfer from the matrix to the intermembrane space (IMS). To increase RC activity according to the chemios ...
... relevant because the pool of small anions in the mitochondrial matrix is limited compared with the cytosolic K+ pool. To compensate for charge movement, the RC must increase the rate of proton transfer from the matrix to the intermembrane space (IMS). To increase RC activity according to the chemios ...
DNA Replication Origin Function Is Promoted by H3K4 Di
... that both Set1 and Bre1 are required for efficient minichromosome maintenance. These phenotypes are recapitulated in yeast strains bearing mutations in the histone substrates (H3K4 and H2BK123). Set1 functions as part of the COMPASS complex to mono-, di-, and tri-methylate H3K4. By analyzing strains ...
... that both Set1 and Bre1 are required for efficient minichromosome maintenance. These phenotypes are recapitulated in yeast strains bearing mutations in the histone substrates (H3K4 and H2BK123). Set1 functions as part of the COMPASS complex to mono-, di-, and tri-methylate H3K4. By analyzing strains ...
Neutrophils in the innate immune response
... ribosomes and mitochondria7. Azurophilic and specific granules are formed during the promyelocyte and myelocyte stages of differentiation, respectively7. As myeloid precursors become mature neutrophils, they sequentially acquire the receptors and proteins needed for innate host defense (reviewed by ...
... ribosomes and mitochondria7. Azurophilic and specific granules are formed during the promyelocyte and myelocyte stages of differentiation, respectively7. As myeloid precursors become mature neutrophils, they sequentially acquire the receptors and proteins needed for innate host defense (reviewed by ...