Mobile Factories: Golgi dynamics in plant cells
... The discussion about models of Golgi movement has unexpected connections to another debate that is currently occupying Golgi researchers. This debate concerns the mode of intra-Golgi transport which in principle can occur either by vesicular shuttles between stable cisternae, or by cisternal progres ...
... The discussion about models of Golgi movement has unexpected connections to another debate that is currently occupying Golgi researchers. This debate concerns the mode of intra-Golgi transport which in principle can occur either by vesicular shuttles between stable cisternae, or by cisternal progres ...
PDF
... trichostatin A (TSA) (Sigma-Aldrich) at a final concentration of 100 nM, or an identical amount of ethanol (control), was added 24 hours before collecting cell lysates. For stable transfections, cells were selected using 400 μg/ml G418 and individual clones were harvested and amplified prior to anal ...
... trichostatin A (TSA) (Sigma-Aldrich) at a final concentration of 100 nM, or an identical amount of ethanol (control), was added 24 hours before collecting cell lysates. For stable transfections, cells were selected using 400 μg/ml G418 and individual clones were harvested and amplified prior to anal ...
Chromatin Dynamics during Lytic Infection with Herpes Simplex
... chain of nucleosomes. Each nucleosome is composed of 165 bp of DNA wrapped 1.46 times around an octamer of core histones H2A, H2B, H3 and H4. The core nucleosome is bound by linker histone H1, for a total of 200 bp and ~2 turns. The linear chain of nucleosomes is then folded into complex higher orde ...
... chain of nucleosomes. Each nucleosome is composed of 165 bp of DNA wrapped 1.46 times around an octamer of core histones H2A, H2B, H3 and H4. The core nucleosome is bound by linker histone H1, for a total of 200 bp and ~2 turns. The linear chain of nucleosomes is then folded into complex higher orde ...
the article
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
... to serve as an ‘architectural’ protein22. As it is now appreciated that HMGB1 can be released from cells, we hypothesized that in a way analogous to its binding of nuclear DNA, HMGB1 could bind to extracellular DNA and, by forming HMGB1-DNA complexes, modify the immune-regulatory effects of specific ...
The Role of PME-1 in Cancer: Therapeutic Implications
... Protein phosphorylation plays an important role in the cellular signaling, by conveying cell proliferation, survival, and death signals across the cellular machinery. Protein kinases and phosphatases regulate the phosphorylation status of thousands of proteins in a cell, and thereby maintain a state ...
... Protein phosphorylation plays an important role in the cellular signaling, by conveying cell proliferation, survival, and death signals across the cellular machinery. Protein kinases and phosphatases regulate the phosphorylation status of thousands of proteins in a cell, and thereby maintain a state ...
PDF - Journal of Neuroscience
... The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquis ...
... The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquis ...
The RNase P Associated with HeLa Cell Mitochondria Contains an
... To test the capacity of mitochondrial precursors to function as substrates for the mtRNase P and nuRNase P, an artificial mitochondrial tRNASer(UCN) precursor [ptRNASer(UCN)] was cloned in the vector pGEM.4Z (Promega). For this purpose, a segment of HeLa cell mtDNA encompassing the whole tRNASer(UCN ...
... To test the capacity of mitochondrial precursors to function as substrates for the mtRNase P and nuRNase P, an artificial mitochondrial tRNASer(UCN) precursor [ptRNASer(UCN)] was cloned in the vector pGEM.4Z (Promega). For this purpose, a segment of HeLa cell mtDNA encompassing the whole tRNASer(UCN ...
Profilin regulates the activity of p42 , a novel Myb
... p42POP is a widely expressed nuclear protein p42POP expression was analysed by using quantitative PCR and subsequent Southern blot on standardized samples of various embryonic stages and adult mouse tissues. POP transcripts were identified in all adult tissues tested and were already present in mous ...
... p42POP is a widely expressed nuclear protein p42POP expression was analysed by using quantitative PCR and subsequent Southern blot on standardized samples of various embryonic stages and adult mouse tissues. POP transcripts were identified in all adult tissues tested and were already present in mous ...
Protozoa - Dr Magrann
... Macronuclei – polyploid (contains DNA and RNA), is involved with differentiation, regeneration and daily activities of cell. No role in sexual repro. ...
... Macronuclei – polyploid (contains DNA and RNA), is involved with differentiation, regeneration and daily activities of cell. No role in sexual repro. ...
Dissecting sterol function during clathrin-dependent
... (DRP1A) contribute to cytokinesis. KNOLLE mediates fusion of vesicles at the plane of cell division while DRP1A appears to be involved in cell plate maturation through its role in clathrin-mediated endocytosis. This thesis shows that KNOLLE is specifically restricted to the cell division plane throu ...
... (DRP1A) contribute to cytokinesis. KNOLLE mediates fusion of vesicles at the plane of cell division while DRP1A appears to be involved in cell plate maturation through its role in clathrin-mediated endocytosis. This thesis shows that KNOLLE is specifically restricted to the cell division plane throu ...
Roles of abscisic acid and auxin in shoot-supplied ammonium inhibition Baohai Li,
... 13. Deak KI, Malamy J. Osmotic regulation of root system architecture. Plant J 2005; 43:17-28; PMID:15960613; DOI:10.1111/j.1365-313X.2005.02425.x. 14. Xiong L, Wang R, Mao G, Koczan JM. Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abs ...
... 13. Deak KI, Malamy J. Osmotic regulation of root system architecture. Plant J 2005; 43:17-28; PMID:15960613; DOI:10.1111/j.1365-313X.2005.02425.x. 14. Xiong L, Wang R, Mao G, Koczan JM. Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abs ...
Bioactive Compounds from Streptomyces nasri and its Mutants with
... of parent Streptomyces strain and UV as well as AO mutants cultivated in three different media. All mutants were able to grow in the three tested culture media with distinguishable differences in EPS production. The response of the parent type of S. nasri and its UV- as well as AO-mutants to changes ...
... of parent Streptomyces strain and UV as well as AO mutants cultivated in three different media. All mutants were able to grow in the three tested culture media with distinguishable differences in EPS production. The response of the parent type of S. nasri and its UV- as well as AO-mutants to changes ...
Drosophila embryos close epithelial wounds using a combination of
... organism does not necessarily define the repair mechanism used, as purse string-mediated repair has been observed in certain adult tissues, including the adult cornea (Danjo and Gipson, 1998). Some types of epithelial cells in culture repair their wounds using a combination of purse string contracti ...
... organism does not necessarily define the repair mechanism used, as purse string-mediated repair has been observed in certain adult tissues, including the adult cornea (Danjo and Gipson, 1998). Some types of epithelial cells in culture repair their wounds using a combination of purse string contracti ...
gauze bandages with a bound antimicrobial polymer suppress
... disadvantage of gauze bandages is the absorption of exudates into the dressing. Exudates absorption contributes to the development of high levels of bacteria in the dressing. A new gauze bandage with a bound antimicrobial polymer was used instead of standard gauze bandages in the treatment of three ...
... disadvantage of gauze bandages is the absorption of exudates into the dressing. Exudates absorption contributes to the development of high levels of bacteria in the dressing. A new gauze bandage with a bound antimicrobial polymer was used instead of standard gauze bandages in the treatment of three ...
Hepatitis A Virus Adaptation to Cellular Shutoff Is Driven by Dynamic
... specific condition of AMD, infected cells (multiplicity of infection [MOI] of 1) from a T-175 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40, pH 7.4), and incubated for 30 min at room temperature. These ...
... specific condition of AMD, infected cells (multiplicity of infection [MOI] of 1) from a T-175 flask were harvested by trypsin treatment, collected by centrifugation, resuspended in 500 l of NT buffer (0.1 M NaCl, 10 mM Tris-HCl, 1% NP-40, pH 7.4), and incubated for 30 min at room temperature. These ...
- Wiley Online Library
... exhaustive CLSM observation of root samples confirmed that V. dahliae used macro- (several cell layers) and micro- (a single cell) wounds as preferred penetration sites within olive root tissues (data not shown). Conidia or hyphal penetration, via either the intercellular space between two epidermal ...
... exhaustive CLSM observation of root samples confirmed that V. dahliae used macro- (several cell layers) and micro- (a single cell) wounds as preferred penetration sites within olive root tissues (data not shown). Conidia or hyphal penetration, via either the intercellular space between two epidermal ...
Coordination of Cellular Pattern-Generating Circuits that Control
... Each local three-cell circuit that controls a swimmeret can be considered as a local oscillator that has its own intrinsic frequency, . The activity of this oscillator can be described by one variable, , the phase of this oscillator as it moves around its stable limit-cycle. In a set of similar os ...
... Each local three-cell circuit that controls a swimmeret can be considered as a local oscillator that has its own intrinsic frequency, . The activity of this oscillator can be described by one variable, , the phase of this oscillator as it moves around its stable limit-cycle. In a set of similar os ...
Methylation Status and Expression of Human Telomerase Reverse
... 10) are present. Luciferase assays assessing promoter activity revealed that a 59-bp region (–208 to –150) is required for maximal promoter activity (7). The 5′ end of the hTERT cDNA contains GC-rich sequences, indicating the presence of a CpG island. hTERT expression may be regulated by methylation ...
... 10) are present. Luciferase assays assessing promoter activity revealed that a 59-bp region (–208 to –150) is required for maximal promoter activity (7). The 5′ end of the hTERT cDNA contains GC-rich sequences, indicating the presence of a CpG island. hTERT expression may be regulated by methylation ...
Uptake of Dissolved Organic Selenides by Marine Phytoplankton
... dissolved organic Se-containing compounds may occur where inorganic Se concentrations are limiting. Although organic Se in open ocean waters is thought to be relatively refractory (Cutter and Bruland 1984), in other more productive environments, organic Se may be more labile, with a large fraction b ...
... dissolved organic Se-containing compounds may occur where inorganic Se concentrations are limiting. Although organic Se in open ocean waters is thought to be relatively refractory (Cutter and Bruland 1984), in other more productive environments, organic Se may be more labile, with a large fraction b ...
Processing of 20S prerRNA to 18S ribosomal RNA in yeast requires
... 60S and 40S ribosomal subunits, respectively. These preribosomal subunits are eventually exported to the cytoplasm, where the ®nal assembly and maturation steps occur (for recent and thorough reviews see Kressler et al., 1999; Venema and Tollervey, 1999). Processing intermediates formed during 35S p ...
... 60S and 40S ribosomal subunits, respectively. These preribosomal subunits are eventually exported to the cytoplasm, where the ®nal assembly and maturation steps occur (for recent and thorough reviews see Kressler et al., 1999; Venema and Tollervey, 1999). Processing intermediates formed during 35S p ...
Sphaerotilus natans encrusted with nanoballshaped Fe(III) oxide
... Iron (Fe) exists in divalent or trivalent states within the biosphere depending on the environmental conditions (Cornell & Schwertmann, 2004). Although the abiotic redox changes between Fe(II) and Fe(III) play an important role in redox processes in the environment, microorganisms also significantly ...
... Iron (Fe) exists in divalent or trivalent states within the biosphere depending on the environmental conditions (Cornell & Schwertmann, 2004). Although the abiotic redox changes between Fe(II) and Fe(III) play an important role in redox processes in the environment, microorganisms also significantly ...
Mutations in the non-helical linker segment L1
... Coulombe et al., 1991a; Fuchs et al., 1992). These mice exhibit skin blistering upon mild physical trauma. In the most severe cases, tonofilament clumping and cell degeneration occur in either basal or suprabasal cells, depending upon whether a K14 or a K10 mutant gene is expressed. These features a ...
... Coulombe et al., 1991a; Fuchs et al., 1992). These mice exhibit skin blistering upon mild physical trauma. In the most severe cases, tonofilament clumping and cell degeneration occur in either basal or suprabasal cells, depending upon whether a K14 or a K10 mutant gene is expressed. These features a ...
Monoclonal Antibody Characterization of Two Distant Sites
... might provide binding affinity and specificity to the fibronectin RGD site has been clouded by several major uncertainties. Because the synergy site was identified using only amino-terminal deletion analysis, the final fusion proteins might have had an incorrect conformation in the region of the RGD ...
... might provide binding affinity and specificity to the fibronectin RGD site has been clouded by several major uncertainties. Because the synergy site was identified using only amino-terminal deletion analysis, the final fusion proteins might have had an incorrect conformation in the region of the RGD ...
Ch 4
... – found wherever areolar connective tissue is located. – reduces heat loss through the skin, serves as an energy reserve, supports, protects, and generates considerable heat to help maintain proper body temperature in ...
... – found wherever areolar connective tissue is located. – reduces heat loss through the skin, serves as an energy reserve, supports, protects, and generates considerable heat to help maintain proper body temperature in ...