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Identifying the causal role of a new adipose tissue signalling
Identifying the causal role of a new adipose tissue signalling

... We have established, in recent years, the Oxford CABG Bioresource (OCB),2-5 which builds a unique, very well characterised cohort of patients undergoing cardiac surgery and stores a wide range of tissue samples, including various types of adipose tissue, human vessels, myocardial samples and others. ...
Prolonged Endoplasmic Reticulum Stress in
Prolonged Endoplasmic Reticulum Stress in

... Using adult rat cardiac myocytes, we studied the effects of angiotensin II on the expression of ER chaperones and apoptosis (Figure 4A). Treatment of adult rat cardiac myocytes with angiotensin II (10⫺9 mol/L) for 24 hours induced cardiac myocyte apoptosis along with the inductions of ER chaperones ...
Cosuppression of Eukaryotic Release Factor 1-1
Cosuppression of Eukaryotic Release Factor 1-1

... insertional effect was ruled out by southern analysis on four independent broomhead lines that clearly shows different chromosomal integration sites for each line (Fig. 4). The degradation observed in broomhead plants was specific to plants showing the phenotype as eRF1-1 transcript levels were up-r ...
Lineage-specific stem cells, signals and asymmetries
Lineage-specific stem cells, signals and asymmetries

... symmetric division by repressing cell cycle regulators such as CDKB1;1 and CYCA2. (B) The loss or gain of function of key stomatal regulators can alter epidermal cell patterning. Shown are false-colored confocal microscope images of developing Arabidopsis epidermis from wild-type plants (left), spch ...
Lead detected
Lead detected

... The distribution of lead in root tips of Dianthus carthusianorum was compared in populations from a zinc-lead waste heap in Bolesław near Olkusz and from a natural stand in the Botanical Garden in Lublin. The analyses were made at two developmental stages: seedlings (after 8 days of incubation in 5 ...
Luminescent Calcium Instructions
Luminescent Calcium Instructions

... is the most efficient known in nature! (With about 90 % of the energy released being converted to light): ATPdependent oxidation of luciferin by luciferase results in bioluminescence (Em = 560 nm) that is longer and brighter in presence of CoenzymeA, at neutral and alkaline pH. Bioluminescence is re ...
Perturbation of Polyamine Catabolism Can Strongly Affect Root
Perturbation of Polyamine Catabolism Can Strongly Affect Root

... addition, the importance of PAs in xylem differentiation and cell death has been confirmed by studies of the Arabidopsis mutant of ACAULIS5 (acl5), encoding a thermospermine synthase specifically expressed in the xylem tissues at a precise developmental stage (Knott et al., 2007). This acl5 mutant s ...
Generation of the podocyte and tubular components
Generation of the podocyte and tubular components

... to induction of glomerular fates (Naylor and Jones, 2009). Notch signaling has been found to be required for generation of glomerular and proximal tubular nephron components in several species (McLaughlin et al., 2000; McCright et al., 2001; Cheng et al., 2003; Taelman et al., 2006; Cheng et al., 20 ...
Effect of Growth at Sub-lethal Concentrations of Kanamycin on the
Effect of Growth at Sub-lethal Concentrations of Kanamycin on the

... BW2511-3) over time. From Fig. 3, a significant increase of glucuronic acid content in the isolated capsules was observed for kanamycin-treated strains B23 and BW2511-3 between 10-min and 60-min postkanamycin treatment, with an increase of ≈2.1 folds (105 µg/mL to 225 µg/mL) and ≈2.4 folds (48 µg/mL ...
b-Globin locus control region HS2 and HS3 interact structurally and
b-Globin locus control region HS2 and HS3 interact structurally and

... transgenic mice, they appear to have distinct globin gene targets (10). However, there has been less consistency in studies when individual HSs have been deleted and/or substituted for one another in the context of the complete human locus introduced into transgenic mice. In some cases, core HS dele ...
Meiosis: Its Origin According to the Viral
Meiosis: Its Origin According to the Viral

... only one of a wide array of features that have such a wide distribution within the eukaryotes that they appear to have been present and almost fully developed in the ancestor of all living eukaryotes [3]. These features include the nucleus, endomembrane systems, linear chromo‐ somes with telomeres, ...
PDF - The Corbo Lab
PDF - The Corbo Lab

... In the present study we investigate the cis-regulatory mechanisms underlying the differentiation of the notochord. Previous studies have identified a number of transcription factors important for chordogenesis in vertebrates. These include a mouse forkhead homolog, HNF-3β, the zebrafish floating hea ...
A single mutation in the core domain of the lac repressor reduces
A single mutation in the core domain of the lac repressor reduces

... Background: The lac operon provides cells with the ability to switch from glucose to lactose metabolism precisely when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rea ...
1. GENERAL INTRODUCTION
1. GENERAL INTRODUCTION

... B cells and T cells are not distinguishable on morphological grounds - short lived lymphocytes (few days) - long lived lymphocytes (up to years) ...
Soma-germline asymmetry in the distributions of
Soma-germline asymmetry in the distributions of

... The distinction between soma and germline is essential to the development of multicellular organisms (e.g. Denis and Lacroix, 1993). In the nematode Caenorhabditis elegans, the separation of somatic and germ lineages occurs during the first four cleavages (Fig. 1; Sulston et al., 1983). The zygote, ...
Regulation of Chlamydomonas flagella and ependymal cell motile
Regulation of Chlamydomonas flagella and ependymal cell motile

... (Figure 3A). This was confirmed both by cell counting with a hemoChlamydomonas expresses serine palmitoyltransferase cytometer and by measuring optical absorbance of chlorophyll at and generates plant sphingolipids 450 nm. At a concentration of myriocin as low as 5 nM, approxiThere is no prior infor ...
Systematic comparison of single-chain Fv antibody
Systematic comparison of single-chain Fv antibody

... CD19 and CD22 differentiation antigens. CD22 is not expressed by any other normal tissue being restricted to only normal and malignant B-cells making this a good candidate target ...
Chapter 36- Resource Acquisition and Transport in Vascular Plants
Chapter 36- Resource Acquisition and Transport in Vascular Plants

... 5. Mltfhf.SIIM If you prune a plant's shoot tips, what will be the short-term effect on the plant's branching and leaf area index? For suggested answers, see Appendix A. ...
supplemental movie legends
supplemental movie legends

... forming a ventral connection to the lumen of the dorsal aorta below. The time-lapse covers a period of about 3 hours, with approximately 30 minutes elapsed time per second of movie (QuickTime; 4.2 MB). Each movie frame is a reconstruction from a Z-series of 15-18 planes (with 2 µm between planes) co ...
Extracellular DNA in biofilms
Extracellular DNA in biofilms

... structural component in many biofilms of S. aureus, its role and its structural organization remains enigmatic. Huseby et al have demonstrated that beta toxin, a neutral sphingomyelinase and a virulence factor of S. aureus, forms covalent cross-links to itself in the presence of DNA, producing an in ...
Nitric Oxide Signalling in Plants: Cross
Nitric Oxide Signalling in Plants: Cross

... by activation of SERCA and/or PMCA helps to protect cells from the deleterious effect of NO. The pathophysiological relevance of these processes is outlined in several examples such as the modulation of neuronal excitability or hypertension but has probably paved the way for new roles in normal and ...
Chapter 3.05
Chapter 3.05

... 6-O-sulfation of the second Gal residue, inhibit the transfer of a GlcA residue by GlcAT-I.24 Thus, the sulfation of specific positions of the two Gal residues may regulate the expression level of GAGs by affecting the activity of GlcAT-I, although further experiments including the cloning of sulfot ...
ultrasonic cleaning
ultrasonic cleaning

... presoak cycle to soften the hardened deposits that typically plug the engraved cells of a ceramic anilox roll before ultrasonic cleaning. This step is considered essential to cleaning without damaging the engraved substrate material. It frequently comprises 80 to 90 percent of the total cleaning tim ...
CELL MOTILITY: Spatial and Temporal Regulation of
CELL MOTILITY: Spatial and Temporal Regulation of

... to seconds, yet whole-cell motility can remain persistent for minutes to hours, four orders of magnitude longer. The cell must exert exact control over the nonadditive layers of complexity that such vast differences in size and timescales create. This review explores how the cell can regulate each o ...
Varicella-Zoster Virus-Infected Human Sensory Neurons Are
Varicella-Zoster Virus-Infected Human Sensory Neurons Are

... vesicular rash confined along one or more sensory dermatomes (28). Herpes zoster can be complicated by postherpetic neuralgia (PHN), a severe, chronic pain that may persist for months to years after the initial appearance of the herpes zoster rash (24, 36). Apoptosis or programmed cell death is an e ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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