DNA REPLICATION CHECKPOINT CONTROL 7. Acknowledgments
... residual maternal protein and proceeded to inappropriate mitosis without checkpoint arrest. These data suggest that Pol-alpha is required for the replication checkpoint even after the initiation of bulk DNA synthesis. Similar studies have been carried out recently using spores germinated from diploi ...
... residual maternal protein and proceeded to inappropriate mitosis without checkpoint arrest. These data suggest that Pol-alpha is required for the replication checkpoint even after the initiation of bulk DNA synthesis. Similar studies have been carried out recently using spores germinated from diploi ...
Phage adsorption and lytic propagation in
... phage propagation in lactic acid bacteria. However, this thematic was studied in E. coli phages. In this sense and in concordance with our results, it was demonstrated E. coli phages were not able to propagate in starved cells in a mineral salt medium [13]. In particular, some authors reported phage ...
... phage propagation in lactic acid bacteria. However, this thematic was studied in E. coli phages. In this sense and in concordance with our results, it was demonstrated E. coli phages were not able to propagate in starved cells in a mineral salt medium [13]. In particular, some authors reported phage ...
Analysis of actin function and organization by a series of mutant
... when returned to rich medium. However, act1-135 and act1-313 cells reestablished polarized growth more slowly than wt cells even at 24°C. To check whether the cytokinesis defective mutants also have any defects in achieving polarized growth, we performed the nitrogen starvation experiment on selecte ...
... when returned to rich medium. However, act1-135 and act1-313 cells reestablished polarized growth more slowly than wt cells even at 24°C. To check whether the cytokinesis defective mutants also have any defects in achieving polarized growth, we performed the nitrogen starvation experiment on selecte ...
Motoneurons Derived from Induced Pluripotent Stem Cells Develop
... Author contributions: V.F.R., J.S.T., B.C.S., P.H.C., J.P.F., Y.Z., and K.E. designed research; V.F.R., J.S.T., B.C.S., D.M.P., and J.P.B. performed research; J.K.I. and K.E. contributed unpublished reagents/analytic tools; V.F.R., J.S.T., B.C.S., D.M.P., J.P.B., J.K.I., J.P.F., Y.Z., and K.E. analy ...
... Author contributions: V.F.R., J.S.T., B.C.S., P.H.C., J.P.F., Y.Z., and K.E. designed research; V.F.R., J.S.T., B.C.S., D.M.P., and J.P.B. performed research; J.K.I. and K.E. contributed unpublished reagents/analytic tools; V.F.R., J.S.T., B.C.S., D.M.P., J.P.B., J.K.I., J.P.F., Y.Z., and K.E. analy ...
Bioluminescence Microscopy
... setup, is the camera. Highest quantum efficiencies are currently realized with electron multiplying CCD (EMCCD) cameras. The advantage of this chip construction lays in the fact that the electron multiplication is happening before the read-out. This means that charge (photon induced but also unwante ...
... setup, is the camera. Highest quantum efficiencies are currently realized with electron multiplying CCD (EMCCD) cameras. The advantage of this chip construction lays in the fact that the electron multiplication is happening before the read-out. This means that charge (photon induced but also unwante ...
the selective interruption of nucleolar rna synthesis in hela cells by
... The nucleolus is a specialized and highly organized structure in the cell, whose principal and perhaps only function is the production of ribosomes (1-3) . Nucleolar function can be easily disrupted by agents which interfere with nucleic acid or protein synthesis . Nucleolar synthesis is inhibited m ...
... The nucleolus is a specialized and highly organized structure in the cell, whose principal and perhaps only function is the production of ribosomes (1-3) . Nucleolar function can be easily disrupted by agents which interfere with nucleic acid or protein synthesis . Nucleolar synthesis is inhibited m ...
The Arabidopsis trichome is an active mechanosensory switch
... 2012). As confirmed by standard staining protocols and transmission electron microscopy (TEM), papillae, which appear in our images as scattered fluorescent ovals, are present on much of the aerial trichome but are much reduced in size and surface density in the pliant zone. The appropriateness of t ...
... 2012). As confirmed by standard staining protocols and transmission electron microscopy (TEM), papillae, which appear in our images as scattered fluorescent ovals, are present on much of the aerial trichome but are much reduced in size and surface density in the pliant zone. The appropriateness of t ...
We have found that an H3K27 methyltransferase activity co
... knockdown experiments (I didn't see them in Fig. 3b, but didn't check the supplementary tables since they are very difficult to look up as genes are identified by their ID numbers, which makes really cumbersome to make anything out of them. I'd imagine that a way out of this would be to knockdown NI ...
... knockdown experiments (I didn't see them in Fig. 3b, but didn't check the supplementary tables since they are very difficult to look up as genes are identified by their ID numbers, which makes really cumbersome to make anything out of them. I'd imagine that a way out of this would be to knockdown NI ...
Leaf growth in grasses is determined by the cell division and
... sheath (i.e., the leaf growth zone), which is highly distinct and relatively simply organized (Hu et al. 2005). Thus, the grass leaf presents a good opportunity to study general leaf growth processes in plants. Leaf growth in wheat, as for other plants like rice, barley, maize and sorghum, is one of ...
... sheath (i.e., the leaf growth zone), which is highly distinct and relatively simply organized (Hu et al. 2005). Thus, the grass leaf presents a good opportunity to study general leaf growth processes in plants. Leaf growth in wheat, as for other plants like rice, barley, maize and sorghum, is one of ...
Coers, J, Bernstein-Hanley, I, Grotsky, D
... was generated by taking advantage of the fact that most mouse knockout strains have been made using 129-derived embryonic stem cell lines and subsequently backcrossed to B6 mice, thus creating 129 congenic mice on a B6 background. To make the B6.Ctrq3129 strain, the congenic mouse strain B6.129S2-Ir ...
... was generated by taking advantage of the fact that most mouse knockout strains have been made using 129-derived embryonic stem cell lines and subsequently backcrossed to B6 mice, thus creating 129 congenic mice on a B6 background. To make the B6.Ctrq3129 strain, the congenic mouse strain B6.129S2-Ir ...
High-throughput functional genomics using
... transcriptional modulation, the modification of expression (white arrows) depends on the exact type of fusion of either dCas9 or sgRNA (part d) (FIG. 2). These induced nuclear events, together with endogenous transcript degradation and dilution through cell division, will result in a new steady-stat ...
... transcriptional modulation, the modification of expression (white arrows) depends on the exact type of fusion of either dCas9 or sgRNA (part d) (FIG. 2). These induced nuclear events, together with endogenous transcript degradation and dilution through cell division, will result in a new steady-stat ...
Electron Microscopic Study of the Guinea Pig
... irregularly shaped, and often elongated; they contained many cytoplasmic processes and large, occasionally deeply indented nuclei. Fig. 8, a section of s.c. tumor and Figs. 3 and 9, areas of lung infiltrated by leukemic cells, illustrate several typical cells and some of their characteristic feature ...
... irregularly shaped, and often elongated; they contained many cytoplasmic processes and large, occasionally deeply indented nuclei. Fig. 8, a section of s.c. tumor and Figs. 3 and 9, areas of lung infiltrated by leukemic cells, illustrate several typical cells and some of their characteristic feature ...
Inflammation and proliferation – a causal event of host response to
... activation of various chemokine mediators, including NFkB, the master regulator of inflammation. H. pylori infection is also associated with an increase in expression of cell cycle regulators, thereby leading to mucosal cell hyper-proliferation. Thus, H. pylori-associated infections manifest activat ...
... activation of various chemokine mediators, including NFkB, the master regulator of inflammation. H. pylori infection is also associated with an increase in expression of cell cycle regulators, thereby leading to mucosal cell hyper-proliferation. Thus, H. pylori-associated infections manifest activat ...
Inter-specific scaling of phytoplankton production and cell size in the
... photosynthesis in the ocean is approximately isometrical (Marañón et al., 2007). A likely explanation for this departure from the 34-power rule lies in the existence of several strategies that allow larger phytoplankton to overcome the geometrical constraints imposed by cell size on resource acqui ...
... photosynthesis in the ocean is approximately isometrical (Marañón et al., 2007). A likely explanation for this departure from the 34-power rule lies in the existence of several strategies that allow larger phytoplankton to overcome the geometrical constraints imposed by cell size on resource acqui ...
Cellular source and molecular form of TNF specify
... Figure 1. Maintenance of spleen microarchitecture is primarily dependent on TNF expressed by B cells with distinct contribution of T-cell–derived TNF. (A) Mice were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, ...
... Figure 1. Maintenance of spleen microarchitecture is primarily dependent on TNF expressed by B cells with distinct contribution of T-cell–derived TNF. (A) Mice were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, ...
... 17]. Furthermore, it is not restricted to a single cell function but covers most, if not all, responses a given cell is capable of such as the respiratory burst, toxicity to host tissue and invading parasites, degranulation, production of lipid mediators, adhesion, and, as shown by BOOMARS et al. [9 ...
Fibroblast growth factor during mesoderm induction
... for bFGF biological activity (Seno et al. 1988) and is not conserved either in chicken or Xenopus [the chicken Ser78 is substituted in Xenopus by Thr (Kimmelman and Kirschner, 1987)]. The change of Ser94 to Leu is conserved both in chicken and Xenopus. Tyr82, which is conserved in all bFGF genes ana ...
... for bFGF biological activity (Seno et al. 1988) and is not conserved either in chicken or Xenopus [the chicken Ser78 is substituted in Xenopus by Thr (Kimmelman and Kirschner, 1987)]. The change of Ser94 to Leu is conserved both in chicken and Xenopus. Tyr82, which is conserved in all bFGF genes ana ...
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... connexió immediata, per la complicitat i amistat sincera, a més de per la muntanya que encara hem de començar a fer junts. Thanks to my New York girls: Gracias a Carolina (la Pola), por ser la alegría de la huerta y por casi llevarme al huerto, además de por tu amistad. Gracias a Laura, todo-corazón ...
... connexió immediata, per la complicitat i amistat sincera, a més de per la muntanya que encara hem de començar a fer junts. Thanks to my New York girls: Gracias a Carolina (la Pola), por ser la alegría de la huerta y por casi llevarme al huerto, además de por tu amistad. Gracias a Laura, todo-corazón ...
Stress signaling from the lumen of the endoplasmic reticulum
... within the cell that continuously responds to environmental cues to release calcium. The ER is exquisitely sensitive to alterations in homeostasis, where, upon a variety of different stimuli, signals are transduced from the ER to the cytoplasm and the nucleus to eventually result in adaptation for s ...
... within the cell that continuously responds to environmental cues to release calcium. The ER is exquisitely sensitive to alterations in homeostasis, where, upon a variety of different stimuli, signals are transduced from the ER to the cytoplasm and the nucleus to eventually result in adaptation for s ...
Plant immune and growth receptors share common signalling
... hetero-oligomers (Kusumi et al., 2011), e.g. FLS2- or BRI1-SERK3/BAK1 complexes. In plants, the cell wall has additional influence on the PM organisation and dynamics (Martinière et al., 2012). As a consequence, lateral mobility and distribution of lipids and proteins within the PM is highly hetero ...
... hetero-oligomers (Kusumi et al., 2011), e.g. FLS2- or BRI1-SERK3/BAK1 complexes. In plants, the cell wall has additional influence on the PM organisation and dynamics (Martinière et al., 2012). As a consequence, lateral mobility and distribution of lipids and proteins within the PM is highly hetero ...
Molecular Microbioiogy
... components isolated from chvB mutants did not possess rhicadhesin activity, whereas cell surface preparations isolated from A. tumefaciens wild-type and plasmid-cured cells did. The same results were obtained with inhibition of attachment of R leguminosarum 248. This result indicates that no active ...
... components isolated from chvB mutants did not possess rhicadhesin activity, whereas cell surface preparations isolated from A. tumefaciens wild-type and plasmid-cured cells did. The same results were obtained with inhibition of attachment of R leguminosarum 248. This result indicates that no active ...
the depression of phagocytosis by exogenous cyclic nucleotides
... capacity for uptake by control cells had nearly reached its maximum, the inhibition of phagocytosis tended to be relieved . Although the amount of inhibition produced by 5 .0 mM theophylline and 5 .0 mM dibutyryl cAMP remained significant even 60 .0 min after cells had been presented with particles, ...
... capacity for uptake by control cells had nearly reached its maximum, the inhibition of phagocytosis tended to be relieved . Although the amount of inhibition produced by 5 .0 mM theophylline and 5 .0 mM dibutyryl cAMP remained significant even 60 .0 min after cells had been presented with particles, ...
Bacteria-host relationship: ubiquitin ligases as weapons of
... Ub ligase mimicry in the ‘arms race’ between pathogen and host Pseudomonas syringae is a pathogen of tomato and Arabidopsis thaliana cells and dedicates about 7% of its genome for producing effectors that dampen host innate immunity and promote disease in plants [28]. Plant cells, in turn, recognize ...
... Ub ligase mimicry in the ‘arms race’ between pathogen and host Pseudomonas syringae is a pathogen of tomato and Arabidopsis thaliana cells and dedicates about 7% of its genome for producing effectors that dampen host innate immunity and promote disease in plants [28]. Plant cells, in turn, recognize ...
Mapping the Synthetic Dosage Lethality Network of CDK1/CDC28
... an essential cellular process (Dixon et al. 2009). Therefore, synthetic lethality can be used to identify new regulators of specific cellular processes, or assign new functions to genes (Enserink 2012). For instance, we recently reported an SGA screen in which we used low doses of 1-NM-PP1 that part ...
... an essential cellular process (Dixon et al. 2009). Therefore, synthetic lethality can be used to identify new regulators of specific cellular processes, or assign new functions to genes (Enserink 2012). For instance, we recently reported an SGA screen in which we used low doses of 1-NM-PP1 that part ...
The nucleolus and herpesviral usurpation
... CSIG), and B23 (Pederson & Tsai, 2009). N-acetyltransferase 10 (NAT10), which has been shown to play a role in maintaining or enhancing the stability of a-tubulin, also influences the cell cycle. The depletion of NAT10 induces defects in nucleolar assembly and cytokinesis and decreases acetylated a- ...
... CSIG), and B23 (Pederson & Tsai, 2009). N-acetyltransferase 10 (NAT10), which has been shown to play a role in maintaining or enhancing the stability of a-tubulin, also influences the cell cycle. The depletion of NAT10 induces defects in nucleolar assembly and cytokinesis and decreases acetylated a- ...