Asymmetric cell division and axis formation in the embryo
... the sperm entry site was altered established that a sperm component confers posterior character to the zygote (Goldstein and Hird, 1996). What is the nature of this symmetry-breaking cue? The cue does not appear to be the sperm entry site or paternally-contributed DNA (Goldstein and Hird, 1996; Sadl ...
... the sperm entry site was altered established that a sperm component confers posterior character to the zygote (Goldstein and Hird, 1996). What is the nature of this symmetry-breaking cue? The cue does not appear to be the sperm entry site or paternally-contributed DNA (Goldstein and Hird, 1996; Sadl ...
BMP and Delta/Notch signaling control the development of
... undergo metamorphosis and lose many chordate characteristics as they become sessile adults. Therefore, it was traditionally thought that amphioxus is the sister group of the vertebrates and that tunicates compose the basal chordate group; however, recent phylogenomic analyses have reverted their pos ...
... undergo metamorphosis and lose many chordate characteristics as they become sessile adults. Therefore, it was traditionally thought that amphioxus is the sister group of the vertebrates and that tunicates compose the basal chordate group; however, recent phylogenomic analyses have reverted their pos ...
First description of giant Archaea (Thaumarchaeota)
... 18S rDNA polymerase chain reaction (PCR) amplifications. The presence of a gene indicative for ammonia oxidation (AmoA) has been tested using rDNA PCR amplifications with universal primers, but all our results were negative. So, at this time, we have no evidence to state if the two giant Thaumarchae ...
... 18S rDNA polymerase chain reaction (PCR) amplifications. The presence of a gene indicative for ammonia oxidation (AmoA) has been tested using rDNA PCR amplifications with universal primers, but all our results were negative. So, at this time, we have no evidence to state if the two giant Thaumarchae ...
Address for Correspondence : VASaks
... responsible for the intracellular localization of organelles in many types of the cells, but their connection to mitochondria and role in regulation of mitochondrial function in muscle cells is still unknown. Microtubules are dynamic structures which depend on many factors, such as temperature and i ...
... responsible for the intracellular localization of organelles in many types of the cells, but their connection to mitochondria and role in regulation of mitochondrial function in muscle cells is still unknown. Microtubules are dynamic structures which depend on many factors, such as temperature and i ...
Role of GATA factors in development, differentiation, and
... Metazoa, display a biased expansion of this subgroup of GATA factors (47). Embryogenesis in C. elegans (reviewed in Ref. 99) begins when the asymmetric first cleavage produces a large anterior daughter called AB and a smaller posterior daughter called P1. P1 then divides to produce the anterior germ ...
... Metazoa, display a biased expansion of this subgroup of GATA factors (47). Embryogenesis in C. elegans (reviewed in Ref. 99) begins when the asymmetric first cleavage produces a large anterior daughter called AB and a smaller posterior daughter called P1. P1 then divides to produce the anterior germ ...
Where in the Cell Are You? Probing HIV
... this are not yet fully elucidated, the microtubule organizing centre (MTOC) can polarize to the synapse and likely plays an important role in synapse formation [18]. Furthermore, actin polymerization has also been reported at the virological synapse [16]. Synapses are not only observed between T cel ...
... this are not yet fully elucidated, the microtubule organizing centre (MTOC) can polarize to the synapse and likely plays an important role in synapse formation [18]. Furthermore, actin polymerization has also been reported at the virological synapse [16]. Synapses are not only observed between T cel ...
AML1(7/7) embryos do not express certain hematopoiesis
... The AML1 and PEBP2b/CBFb genes encode the DNAbinding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of de®nitive hematopoiesis in the fetal liver on embryonic day 11. ...
... The AML1 and PEBP2b/CBFb genes encode the DNAbinding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of de®nitive hematopoiesis in the fetal liver on embryonic day 11. ...
Correlative Fluorescence and Electron Microscopy on Ultrathin
... sites by confocal and electron microscopy. HeLa cells were permeabilized, allowed to extend nascent transcripts in Br-UTP for 15 min, fixed, and cryosections (150 nm) prepared. One cryosection was transferred onto a glass coverslip, Br-RNA indirectly immunolabeled with FITC–protein A adsorbed to 6- ...
... sites by confocal and electron microscopy. HeLa cells were permeabilized, allowed to extend nascent transcripts in Br-UTP for 15 min, fixed, and cryosections (150 nm) prepared. One cryosection was transferred onto a glass coverslip, Br-RNA indirectly immunolabeled with FITC–protein A adsorbed to 6- ...
RBM3 regulates temperature sensitive miR-142–5p and miR
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re- ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re- ...
medline:93194904
... Cell type- and stage-specific expression of the CD20/B1 antigen correlates with the activity of a diverged octamer DNA motif present in its promoter. The CD20(B1) encodes a B cell-specific protein involved in the regulation of human B cell proliferation and differentiation. Studies with 5' deletion ...
... Cell type- and stage-specific expression of the CD20/B1 antigen correlates with the activity of a diverged octamer DNA motif present in its promoter. The CD20(B1) encodes a B cell-specific protein involved in the regulation of human B cell proliferation and differentiation. Studies with 5' deletion ...
HEMOLYTIC ANEMIA
... Fc receptors/C3b recognized by macrophages; therefore, • Hemolysis primarily extravascular • 70% associated with other illnesses • Responsive to steroids/splenectomy ...
... Fc receptors/C3b recognized by macrophages; therefore, • Hemolysis primarily extravascular • 70% associated with other illnesses • Responsive to steroids/splenectomy ...
Primary and immortalized mouse epicardial cells undergo
... Smads 2 and 3 (Kretzschmar and Massague, 1998). These activated RSmads complex with Smad 4 and translocate into the nucleus to alter gene transcription. TGF can activate additional downstream effectors including RhoA (Bhowmick et al., 2001a, 2003; Edlund et al., 2002; Masszi et al., 2003; Deaton et ...
... Smads 2 and 3 (Kretzschmar and Massague, 1998). These activated RSmads complex with Smad 4 and translocate into the nucleus to alter gene transcription. TGF can activate additional downstream effectors including RhoA (Bhowmick et al., 2001a, 2003; Edlund et al., 2002; Masszi et al., 2003; Deaton et ...
Microtubule-associated protein 1b is required for shaping the neural
... MAP1B, a founding member of this family, is posttranslationally cleaved into a heavy chain (HC) and a light chain (LC1) [24]. The heavy chain contains domains for actin, MTs and LC1 binding [25–27] and can therefore crosslink MTs and microfilaments [28, 29]. The light chain also binds MTs and actin ...
... MAP1B, a founding member of this family, is posttranslationally cleaved into a heavy chain (HC) and a light chain (LC1) [24]. The heavy chain contains domains for actin, MTs and LC1 binding [25–27] and can therefore crosslink MTs and microfilaments [28, 29]. The light chain also binds MTs and actin ...
Like-acetylglucosaminyltransferase (LARGE)
... sites on α-dystroglycan, we generated deletions or point mutations in the first half of the mucin-like domain, where LARGEdependent modification of α-dystroglycan takes place (16). Because the DGN is necessary for the interaction with LARGE, all constructs were designed to include it. All mutants were ...
... sites on α-dystroglycan, we generated deletions or point mutations in the first half of the mucin-like domain, where LARGEdependent modification of α-dystroglycan takes place (16). Because the DGN is necessary for the interaction with LARGE, all constructs were designed to include it. All mutants were ...
Proteins that contain a functional Z-DNA
... in their localization to stress granules. We additionally show that the Za domain from other Z-DNAbinding proteins (ZBP1, E3L) is likewise sufficient for localization to stress granules. Finally, we show that Z-RNA or Z-DNA binding is important for stress granule localization. We have thus identifie ...
... in their localization to stress granules. We additionally show that the Za domain from other Z-DNAbinding proteins (ZBP1, E3L) is likewise sufficient for localization to stress granules. Finally, we show that Z-RNA or Z-DNA binding is important for stress granule localization. We have thus identifie ...
Developmental stage‐specific expression of Rbm suggests its
... We therefore wished to use immunohistochemistry to determine the localization pattern of Rbm protein in the testis. To this end, antisera were raised against the bacterially expressed Rbm fusion protein and were af®nity-puri®ed. Immunoblot analysis revealed a single band of 43 kDa in size that, amon ...
... We therefore wished to use immunohistochemistry to determine the localization pattern of Rbm protein in the testis. To this end, antisera were raised against the bacterially expressed Rbm fusion protein and were af®nity-puri®ed. Immunoblot analysis revealed a single band of 43 kDa in size that, amon ...
LETTERS
... TopIB-linked nick. Figure 2b–e shows a collection of traces illustrating the assignment of tstart and tend. The majority of events observed (,70%) resemble those in Fig. 2b, c. In Fig. 2b, at ,340 s, the magnets are rotated for a substantial number of turns on two occasions (the interruption of the ...
... TopIB-linked nick. Figure 2b–e shows a collection of traces illustrating the assignment of tstart and tend. The majority of events observed (,70%) resemble those in Fig. 2b, c. In Fig. 2b, at ,340 s, the magnets are rotated for a substantial number of turns on two occasions (the interruption of the ...
Cosuppression of Eukaryotic Release Factor 1-1
... insertional effect was ruled out by southern analysis on four independent broomhead lines that clearly shows different chromosomal integration sites for each line (Fig. 4). The degradation observed in broomhead plants was specific to plants showing the phenotype as eRF1-1 transcript levels were up-r ...
... insertional effect was ruled out by southern analysis on four independent broomhead lines that clearly shows different chromosomal integration sites for each line (Fig. 4). The degradation observed in broomhead plants was specific to plants showing the phenotype as eRF1-1 transcript levels were up-r ...
PDF
... To prevent the formation of ectodermal bandlets, mesodermal bandlets or entire germinal bands, teloblasts and/or proteloblasts were injected with a mixture of 10.8 µg/ml of the A chain of lectin ricin (Sigma), 7.5 mg/ml FDA and 0.4% Fast Green in 0.2 M KCl. In mammalian cells, the ricin A chain modi ...
... To prevent the formation of ectodermal bandlets, mesodermal bandlets or entire germinal bands, teloblasts and/or proteloblasts were injected with a mixture of 10.8 µg/ml of the A chain of lectin ricin (Sigma), 7.5 mg/ml FDA and 0.4% Fast Green in 0.2 M KCl. In mammalian cells, the ricin A chain modi ...
A leucine aminopeptidase is involved in kinetoplast DNA
... assembled with a region of non-corrugated inner mt membrane between them [2]. Unlike the mt DNA of most eukaryotes, kDNA divides once per cell cycle. Whilst this occurs immediately prior to nuclear DNA synthesis, replication of kDNA is in synchrony with nuclear division [3]. In T. brucei, kDNA is es ...
... assembled with a region of non-corrugated inner mt membrane between them [2]. Unlike the mt DNA of most eukaryotes, kDNA divides once per cell cycle. Whilst this occurs immediately prior to nuclear DNA synthesis, replication of kDNA is in synchrony with nuclear division [3]. In T. brucei, kDNA is es ...
Purine nucleosides: endogenous neuroprotectants in hypoxic brain
... 2011; Zhang et al. 2011). Purine nucleoside-mediated effects in hypoxia are therefore exceptionally interesting due to their endogenous regulatory mechanisms in stress situations. Growing evidence suggests that purine nucleosides, which may remain elevated for days after the insult (Uemura et al. 19 ...
... 2011; Zhang et al. 2011). Purine nucleoside-mediated effects in hypoxia are therefore exceptionally interesting due to their endogenous regulatory mechanisms in stress situations. Growing evidence suggests that purine nucleosides, which may remain elevated for days after the insult (Uemura et al. 19 ...
Identifying the causal role of a new adipose tissue signalling
... We have established, in recent years, the Oxford CABG Bioresource (OCB),2-5 which builds a unique, very well characterised cohort of patients undergoing cardiac surgery and stores a wide range of tissue samples, including various types of adipose tissue, human vessels, myocardial samples and others. ...
... We have established, in recent years, the Oxford CABG Bioresource (OCB),2-5 which builds a unique, very well characterised cohort of patients undergoing cardiac surgery and stores a wide range of tissue samples, including various types of adipose tissue, human vessels, myocardial samples and others. ...
the Essential Standards of Biology
... Education Authority (LEA) to design the specific curricular and instructional strategies that best deliver the content to their students. Nonetheless, engaging students in inquiry-based instruction is a critical way of developing conceptual understanding of the science content that is vital for succ ...
... Education Authority (LEA) to design the specific curricular and instructional strategies that best deliver the content to their students. Nonetheless, engaging students in inquiry-based instruction is a critical way of developing conceptual understanding of the science content that is vital for succ ...