THE USE OF STABLE ISOTOPES IN BIOLOGICAL
... The reports of these studies form a voluminous in one or more of its constituent units. One can literature. Several reviews (1-7) which deal with trace the fate of the labeled substance, i.e., its disvarious aspects of tracer research have appeared tribution and localization, and one can measure in ...
... The reports of these studies form a voluminous in one or more of its constituent units. One can literature. Several reviews (1-7) which deal with trace the fate of the labeled substance, i.e., its disvarious aspects of tracer research have appeared tribution and localization, and one can measure in ...
effects of disease-causing mutations associated with five
... mislocalized mutants predominantly co-localized with WT Best1 in intracellular compartments. Studies involving four ARB truncation mutants reveal that the first 174 amino acids are sufficient to mediate oligomerization with WT Best1 and that amino acids 472-585 are not necessary for proper trafficki ...
... mislocalized mutants predominantly co-localized with WT Best1 in intracellular compartments. Studies involving four ARB truncation mutants reveal that the first 174 amino acids are sufficient to mediate oligomerization with WT Best1 and that amino acids 472-585 are not necessary for proper trafficki ...
Designing a single-molecule biophysics tool for characterizing DNA
... measure the effectiveness of this class of antibiotics, or to determine the dose required. There are other applications where quantitative measures of DNA damage are required, for example in studying programmed cell death, or for evaluating the effects of novel cancer treatments. For many medical ap ...
... measure the effectiveness of this class of antibiotics, or to determine the dose required. There are other applications where quantitative measures of DNA damage are required, for example in studying programmed cell death, or for evaluating the effects of novel cancer treatments. For many medical ap ...
PDF
... treatments (Table 1). The P-controlled cultures were able to reach (near) P-replete growth rates, but not under all light conditions. For M. pusilla the P-controlled growth rates under ML and LL were similar as under P-replete conditions, but HL led to an approximate 40% growth rate reduction. In co ...
... treatments (Table 1). The P-controlled cultures were able to reach (near) P-replete growth rates, but not under all light conditions. For M. pusilla the P-controlled growth rates under ML and LL were similar as under P-replete conditions, but HL led to an approximate 40% growth rate reduction. In co ...
FOXP3: Of Mice and Men
... These T cells, identified by their expression of CD4, the IL-2Rα chain (CD25), and the forkhead family transcription factor Foxp3, are know as regulatory T cells, or Tregs. They have the ability to inhibit the development of autoimmunity when transferred into the appropriate host. Recent work has sho ...
... These T cells, identified by their expression of CD4, the IL-2Rα chain (CD25), and the forkhead family transcription factor Foxp3, are know as regulatory T cells, or Tregs. They have the ability to inhibit the development of autoimmunity when transferred into the appropriate host. Recent work has sho ...
Unprocessed Proinsulin Promotes Cell Survival During
... low or very high prepancreatic insulin availability, due to altered preproinsulin mRNA regulation induced by factors related to or independent of hyperglycemia, may contribute to the appearence of congenital abnormalities. Low insulin levels were reported to cause retarded growth and development in ...
... low or very high prepancreatic insulin availability, due to altered preproinsulin mRNA regulation induced by factors related to or independent of hyperglycemia, may contribute to the appearence of congenital abnormalities. Low insulin levels were reported to cause retarded growth and development in ...
during Epstein-Barr Virus Reactivation Transcription by Lytic
... expression and disrupting IFN-␥-stimulated signaling pathway, HCMV interferes with MHC class II transcription and CIITA activation. Other strategies for the down-modulation MHC class II surface expression by HCMV are promoting the proteasome-mediated degradation of DR-␣ and DM-␣ molecules by glycopr ...
... expression and disrupting IFN-␥-stimulated signaling pathway, HCMV interferes with MHC class II transcription and CIITA activation. Other strategies for the down-modulation MHC class II surface expression by HCMV are promoting the proteasome-mediated degradation of DR-␣ and DM-␣ molecules by glycopr ...
Emergent Synchronous Bursting of Oxytocin Neuronal Network
... cells and (in virgin rats) about 3.2 ng of oxytocin, about 95% of which is in the dendrites [11]. Oxytocin cells have 2–5 dendrites, several hundred micrometres long, which are filled with neurosecretory vesicles that can also be released by exocytosis [12]. In a virgin rat, each cell has .10,000 ve ...
... cells and (in virgin rats) about 3.2 ng of oxytocin, about 95% of which is in the dendrites [11]. Oxytocin cells have 2–5 dendrites, several hundred micrometres long, which are filled with neurosecretory vesicles that can also be released by exocytosis [12]. In a virgin rat, each cell has .10,000 ve ...
Bio 226: Cell and Molecular Biology
... Course Project = Algal Lipid Production 1)Decide which algae to study http://www.cbs.umn.edu/lab/wackett/links/oil Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? ...
... Course Project = Algal Lipid Production 1)Decide which algae to study http://www.cbs.umn.edu/lab/wackett/links/oil Next assignment: each pick an alga and product and convince the group in 5-10 minutes why your choice is best. Next Wed? ...
Positive and Negative Regulation of Muscle Cell
... development of muscle fiber type identity, we expressed zebrafish sonic hedgehog or tiggy-winkle hedgehog ectopically by injection of RNA into cleavage stage embryos. We then examined the developing embryos for induction of slow muscle cells using several monoclonal antibodies that recognize the ent ...
... development of muscle fiber type identity, we expressed zebrafish sonic hedgehog or tiggy-winkle hedgehog ectopically by injection of RNA into cleavage stage embryos. We then examined the developing embryos for induction of slow muscle cells using several monoclonal antibodies that recognize the ent ...
The Diversity and Molecular Evolution of B-Cell
... The extraordinary variability of BCR sequences poses challenges for targeted sequencing. We provide here only a brief summary of current sequencing approaches, in particular as they relate to the analysis of BCR diversity. Rearranged VDJ segments are flanked by introns, so targeting germline DNA req ...
... The extraordinary variability of BCR sequences poses challenges for targeted sequencing. We provide here only a brief summary of current sequencing approaches, in particular as they relate to the analysis of BCR diversity. Rearranged VDJ segments are flanked by introns, so targeting germline DNA req ...
Cleavage modification did not alter early blastomere fates
... bioRxiv preprint first posted online Aug. 11, 2016; doi: http://dx.doi.org/10.1101/068783. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... bioRxiv preprint first posted online Aug. 11, 2016; doi: http://dx.doi.org/10.1101/068783. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
Escherichia coli DNA ligase B may mitigate damage from
... caused a dramatic extension of lag phase that eventually resumed normal growth. The ligase function of ligase B was not required to mediate the extended lag phase, as overexpression of a ligase-deficient ligB mutant also blocked growth. Overexpression of ligB during logarithmic growth caused an imme ...
... caused a dramatic extension of lag phase that eventually resumed normal growth. The ligase function of ligase B was not required to mediate the extended lag phase, as overexpression of a ligase-deficient ligB mutant also blocked growth. Overexpression of ligB during logarithmic growth caused an imme ...
histology of organogenic and embryogenic responses in cotyledons
... Fig. 1 Cotyledons joined to the embryo axis during 40 d of culture on PGR-free medium (Schenk and Hildebrandt macronutrients; micronutrients, vitamins, and Fe-EDTA from Murashige and Skoog). C = cotyledon; EA = embryo axis; EP = epidermis; SEP = subepidermis; GP = ground parenchyma; N = nucleus; SE ...
... Fig. 1 Cotyledons joined to the embryo axis during 40 d of culture on PGR-free medium (Schenk and Hildebrandt macronutrients; micronutrients, vitamins, and Fe-EDTA from Murashige and Skoog). C = cotyledon; EA = embryo axis; EP = epidermis; SEP = subepidermis; GP = ground parenchyma; N = nucleus; SE ...
Coca Cola
... - Contractile ring during cell division Video 01.1-keratocyte_dance Video 22.7 –neurite_outgrowth ...
... - Contractile ring during cell division Video 01.1-keratocyte_dance Video 22.7 –neurite_outgrowth ...
Primary Mediastinal Seminomas: Evidence of Single KIT
... KIT may reflect a lack of cell surface expression. KIT is required in germ cell migration during embryogenesis (Mauduit et al, 1999). Thus, the lack of purely membranous KIT expression found in the MS may actually support the reason for halt in cell migration to the gonads. Alternatively, this effec ...
... KIT may reflect a lack of cell surface expression. KIT is required in germ cell migration during embryogenesis (Mauduit et al, 1999). Thus, the lack of purely membranous KIT expression found in the MS may actually support the reason for halt in cell migration to the gonads. Alternatively, this effec ...
The humoral pattern recognition receptor PTX3 is stored in
... to nonspecific binding of the anti-PTX3 mAb (not depicted). To confirm this observation, PTX3 expression was analyzed by Western blotting. Human neutrophils and DCs express two or three immunoreactive forms of PTX3, depending on the donor (with one major band at 47 kD and two minor bands at 44 and 4 ...
... to nonspecific binding of the anti-PTX3 mAb (not depicted). To confirm this observation, PTX3 expression was analyzed by Western blotting. Human neutrophils and DCs express two or three immunoreactive forms of PTX3, depending on the donor (with one major band at 47 kD and two minor bands at 44 and 4 ...
Seed-coat Dormancy in Grevillea linearifolia
... doi:10.1093/aob/mcn006, available online at www.aob.oxfordjournals.org ...
... doi:10.1093/aob/mcn006, available online at www.aob.oxfordjournals.org ...
Cholinergic Responses and Intrinsic Membrane Properties of
... Whole cell patch-clamp recordings were performed in 9- to 20-dayold rat Pf neurons in parasagittal slices, and responses to the cholinergic agonist carbachol (CAR) were determined. Three types of responses were identified: inhibitory (55.3%), excitatory (31.1%), and biphasic (fast inhibitory followe ...
... Whole cell patch-clamp recordings were performed in 9- to 20-dayold rat Pf neurons in parasagittal slices, and responses to the cholinergic agonist carbachol (CAR) were determined. Three types of responses were identified: inhibitory (55.3%), excitatory (31.1%), and biphasic (fast inhibitory followe ...
Laser Microdissection and Spatiotemporal Pinoresinol
... of synthesis and accumulation of SDG at a cellular level are presented here. Knowledge of cell-type specific localization of metabolites in seed coats would shed light on the putative ecological function of lignans, especially of the SDG oligomer complex, in defending the seeds against pathogens and ...
... of synthesis and accumulation of SDG at a cellular level are presented here. Knowledge of cell-type specific localization of metabolites in seed coats would shed light on the putative ecological function of lignans, especially of the SDG oligomer complex, in defending the seeds against pathogens and ...
CDK5 is a major regulator of the tumor suppressor DLC1
... To establish whether an endogenous protein complex containing DLC1 and CDK5 exists in vivo, we performed coimmunoprecipitation (co-IP) experiments from two non-small cell lung cancer (NSCLC) lines, H1703 and H157, which expressed both proteins. DLC1 and CDK5 formed a protein complex in both lines (F ...
... To establish whether an endogenous protein complex containing DLC1 and CDK5 exists in vivo, we performed coimmunoprecipitation (co-IP) experiments from two non-small cell lung cancer (NSCLC) lines, H1703 and H157, which expressed both proteins. DLC1 and CDK5 formed a protein complex in both lines (F ...
Transformations of phosphatidylinositol phosphates in the outer and
... PIPs’ must be compartmentalized in a function-dependent manner. Thus, the 18 phosphoinositide interconversion reactions identified thus far and mediated by as many as 47 genes encoding 19 phosphatidylinositide kinases and 28 phosphatidylinositide phosphatases [4], must be restricted by cellular spac ...
... PIPs’ must be compartmentalized in a function-dependent manner. Thus, the 18 phosphoinositide interconversion reactions identified thus far and mediated by as many as 47 genes encoding 19 phosphatidylinositide kinases and 28 phosphatidylinositide phosphatases [4], must be restricted by cellular spac ...
Natural sequence variants of yeast environmental sensors confer
... (e.g., with either low or high trait values) without necessarily changing the mean trait value of all carriers. A PTL may also change the transition rate between two phenotypic states without modifying the overall proportion of individuals being in one state at a given time. Note that many QTLs have ...
... (e.g., with either low or high trait values) without necessarily changing the mean trait value of all carriers. A PTL may also change the transition rate between two phenotypic states without modifying the overall proportion of individuals being in one state at a given time. Note that many QTLs have ...
Bioluminescence Microscopy
... setup, is the camera. Highest quantum efficiencies are currently realized with electron multiplying CCD (EMCCD) cameras. The advantage of this chip construction lays in the fact that the electron multiplication is happening before the read-out. This means that charge (photon induced but also unwante ...
... setup, is the camera. Highest quantum efficiencies are currently realized with electron multiplying CCD (EMCCD) cameras. The advantage of this chip construction lays in the fact that the electron multiplication is happening before the read-out. This means that charge (photon induced but also unwante ...