Kinesin-1–syntaphilin coupling mediates activity
... from snph knockout, mouse brains (Fig. 4 C), providing convincing evidence for a native SNPH–KIF5 complex. Our pulldown and coimmunoprecipitation assays suggest that SNPH interacts with KIF5 independent of KLC. In contrast, a native SNPH–dynein heavy chain (DHC) complex was not detected ...
... from snph knockout, mouse brains (Fig. 4 C), providing convincing evidence for a native SNPH–KIF5 complex. Our pulldown and coimmunoprecipitation assays suggest that SNPH interacts with KIF5 independent of KLC. In contrast, a native SNPH–dynein heavy chain (DHC) complex was not detected ...
O-GlcNAc transferase inhibitors: current tools and
... to as ‘compound 5’ or BZX [35] (Figure 1). The inhibition efficiency of the two molecules was tested in vitro against full-length OGT constructs showing IC50 of 53 μM for compound 4 and 10 μM for compound 5. A study showed that OGT inhibition by compound 4 decreased pancreatic β-cell development and ...
... to as ‘compound 5’ or BZX [35] (Figure 1). The inhibition efficiency of the two molecules was tested in vitro against full-length OGT constructs showing IC50 of 53 μM for compound 4 and 10 μM for compound 5. A study showed that OGT inhibition by compound 4 decreased pancreatic β-cell development and ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... Arabidopsis CesA N-terminal domains. Previously, heterologously-expressed cotton N-terminal domains have been shown to dimerize, in a redox dependent manner, through their zinc binding domains (Kurek et al. 2002). However, assembly of a functional CSC requires interaction between three unique CesA i ...
... Arabidopsis CesA N-terminal domains. Previously, heterologously-expressed cotton N-terminal domains have been shown to dimerize, in a redox dependent manner, through their zinc binding domains (Kurek et al. 2002). However, assembly of a functional CSC requires interaction between three unique CesA i ...
Crosstalk in NF-κB signaling pathways
... transcription1,2. All NF-κB proteins share a Rel homology domain responsible for DNA binding and dimerization. Bacterial and viral infection, inflammatory cytokines and engagement of antigen receptors all elicit NF-κB activation, which highlights the crucial role of this transcription factor in the ...
... transcription1,2. All NF-κB proteins share a Rel homology domain responsible for DNA binding and dimerization. Bacterial and viral infection, inflammatory cytokines and engagement of antigen receptors all elicit NF-κB activation, which highlights the crucial role of this transcription factor in the ...
RNA helicase DDX19 stabilizes ribosomal elongation and
... called RecA-like domains, linked to each other by a flexible linker. Most conserved sequence motifs of this protein are located in the central cleft between two RecA domains, involved in binding and hydrolysis of ATP (3). The RNA is bound by both domains in a sequence-independent manner. The N-termi ...
... called RecA-like domains, linked to each other by a flexible linker. Most conserved sequence motifs of this protein are located in the central cleft between two RecA domains, involved in binding and hydrolysis of ATP (3). The RNA is bound by both domains in a sequence-independent manner. The N-termi ...
Calcium binding to chromaffin vesicle matrix proteins
... and Hepes were used as buffer substances at pH 6.0and 8.0, respectively. All buffers used for dialysis also contained 0.5 g of Chelex-100/200 mL, in order to remove residual Ca2+ before titration. After dialysis, Ca2+concentrations between IOd and IO-’ were determined in the samples. The Ca2+ concen ...
... and Hepes were used as buffer substances at pH 6.0and 8.0, respectively. All buffers used for dialysis also contained 0.5 g of Chelex-100/200 mL, in order to remove residual Ca2+ before titration. After dialysis, Ca2+concentrations between IOd and IO-’ were determined in the samples. The Ca2+ concen ...
curriculum vitae- michael hippler
... and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many proteins of the fermentative metabolism. Comparative quantitative analyses were performed with the chlor ...
... and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting localized 895 of these proteins to the chloroplast, including many proteins of the fermentative metabolism. Comparative quantitative analyses were performed with the chlor ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... minus of the protein encoded by ORF At3 g18480 shows homology to the TM domain of human CASP (58% identity) [24,43]. The similarity between these two proteins is, however, also evident in other regions of the protein. The membrane topologies of At1 g18190, At2 g19950 and At3 g18480 resemble those of ...
... minus of the protein encoded by ORF At3 g18480 shows homology to the TM domain of human CASP (58% identity) [24,43]. The similarity between these two proteins is, however, also evident in other regions of the protein. The membrane topologies of At1 g18190, At2 g19950 and At3 g18480 resemble those of ...
A specific domain in α-catenin mediates binding to β
... Max-Planck Institute for Immunobiology, Stübeweg 51, D-79108 Freiburg, Germany *Author for correspondence (e-mail: [email protected]) ...
... Max-Planck Institute for Immunobiology, Stübeweg 51, D-79108 Freiburg, Germany *Author for correspondence (e-mail: [email protected]) ...
Effects of various oxidants and antioxidants on the p38
... phosphorylated form of the kinase at the Thr and Tyr residues of the Thr-Gly-Tyr motif, since this form is known to be the active one [20]. The results of this study revealed that H2 O2 induced activation of p38-MAPK (7.04 ± 0.20-fold relative to control values; p < 0.001, N = 6) was completely inhi ...
... phosphorylated form of the kinase at the Thr and Tyr residues of the Thr-Gly-Tyr motif, since this form is known to be the active one [20]. The results of this study revealed that H2 O2 induced activation of p38-MAPK (7.04 ± 0.20-fold relative to control values; p < 0.001, N = 6) was completely inhi ...
Bax cleavage is mediated by calpain during drug-induced
... et al., 1996; Squier and Cohen, 1997). Recent data have illustrated the central role of mitochondria in initiating cell death. Upon induction of apoptosis this organelle undergoes a series of changes that are crucial to the death program. One event, the mitochondrial permeability transition (PT), re ...
... et al., 1996; Squier and Cohen, 1997). Recent data have illustrated the central role of mitochondria in initiating cell death. Upon induction of apoptosis this organelle undergoes a series of changes that are crucial to the death program. One event, the mitochondrial permeability transition (PT), re ...
Role of Smad4 (DPC4) inactivation in human cancer.
... SMAD4 mutant mouse embryos exhibit early embryonic lethality • By day 6.5, SMAD4 mutant mouse embryos appear much smaller than wild type ones; the difference in size is attributed to reduced cell ...
... SMAD4 mutant mouse embryos exhibit early embryonic lethality • By day 6.5, SMAD4 mutant mouse embryos appear much smaller than wild type ones; the difference in size is attributed to reduced cell ...
Reverse engineering of drug induced DNA damage response
... resulting network signalling triggered within the cell not only repairs the DNA lesion, but is also tightly linked with the cellular machinery that governs cell-fate decision e.g. cell cycle arrest to promote survival or apoptosis for programmed cell death [5]. Depending upon the scale and type of D ...
... resulting network signalling triggered within the cell not only repairs the DNA lesion, but is also tightly linked with the cellular machinery that governs cell-fate decision e.g. cell cycle arrest to promote survival or apoptosis for programmed cell death [5]. Depending upon the scale and type of D ...
Tracing the Archaeal Origins of Eukaryotic Membrane
... 2007; Dacks et al. 2008). Therefore, despite the apparent complexity of the modern trafficking machinery, the paralogous nature of the proteins involved suggests that this machinery evolved from a smaller set of primordial vesicle formation and fusion proteins that were present in early stages of eu ...
... 2007; Dacks et al. 2008). Therefore, despite the apparent complexity of the modern trafficking machinery, the paralogous nature of the proteins involved suggests that this machinery evolved from a smaller set of primordial vesicle formation and fusion proteins that were present in early stages of eu ...
Cleavage furrow formation and ingression during animal cytokinesis
... be able to induce furrowing, and the contribution of each component may vary depending upon the organism and cell type, as previously proposed (Wang, 2001). Drosophila spermatocytes and tissue culture cells may exemplify a general mechanism, in which astral microtubules initiate furrowing and then s ...
... be able to induce furrowing, and the contribution of each component may vary depending upon the organism and cell type, as previously proposed (Wang, 2001). Drosophila spermatocytes and tissue culture cells may exemplify a general mechanism, in which astral microtubules initiate furrowing and then s ...
A GH3-like Domain in Reaper Is Required for Mitochondrial
... Downloaded from www.jbc.org at Yale University on December 17, 2008 ...
... Downloaded from www.jbc.org at Yale University on December 17, 2008 ...
Up-regulation of junctophilin-2 prevents ER stress and apoptosis in
... Ischemic heart disease is the leading cause of death, and reperfusion which can restore blood flow is the primary therapy. However, reperfusion can induce further damage to cardiomyocytes, a condition described as ischemia-reperfusion (I/R) injury. I/R is now recognized as a combination determining ...
... Ischemic heart disease is the leading cause of death, and reperfusion which can restore blood flow is the primary therapy. However, reperfusion can induce further damage to cardiomyocytes, a condition described as ischemia-reperfusion (I/R) injury. I/R is now recognized as a combination determining ...
structure-function analysis of the drosophila stubble type ii
... Studies of human disease have typically been undertaken in cell culture and mammalian model systems. Although these systems are more amenable to genetic manipulation than humans themselves, they have significant limitations. Studies in cell culture suffer from the disadvantage that cells are usually ...
... Studies of human disease have typically been undertaken in cell culture and mammalian model systems. Although these systems are more amenable to genetic manipulation than humans themselves, they have significant limitations. Studies in cell culture suffer from the disadvantage that cells are usually ...
Role of hsp90 and the hsp90-binding immunophilins in signalling
... affect a client protein function (i.e. steroid binding) through its peptidylprolyl isomerase activity. Presumably, the folding change due to isomerization occurs in the client protein itself, although that remains to be demonstrated. It should be noted that GR that has been assembled into GR hsp90 h ...
... affect a client protein function (i.e. steroid binding) through its peptidylprolyl isomerase activity. Presumably, the folding change due to isomerization occurs in the client protein itself, although that remains to be demonstrated. It should be noted that GR that has been assembled into GR hsp90 h ...
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an
... Fas ligand), the absence of survival factors (fibroblast growth factors (FGFs), IGF-I, and neurotrophins), genotoxic stresses and activation of the oncosuppressor p53. Whatever the original death signal, most apoptotic pathways converge on activation of the caspase cascade, which leads to cell degrad ...
... Fas ligand), the absence of survival factors (fibroblast growth factors (FGFs), IGF-I, and neurotrophins), genotoxic stresses and activation of the oncosuppressor p53. Whatever the original death signal, most apoptotic pathways converge on activation of the caspase cascade, which leads to cell degrad ...
Article Title Authors Author affiliation Correspondence information
... observed here was autophagic in nature, and thus was regulated by JNK pathway [15]. It has been reported anti-tumor reagents, including arsenic trioxide and ǻ9-THC (tetrahydrocannabinol) can induce autophagy without triggering caspase-dependent apoptosis [48-50]. In recent years, it has been propose ...
... observed here was autophagic in nature, and thus was regulated by JNK pathway [15]. It has been reported anti-tumor reagents, including arsenic trioxide and ǻ9-THC (tetrahydrocannabinol) can induce autophagy without triggering caspase-dependent apoptosis [48-50]. In recent years, it has been propose ...
DOMAIN-BASED APPROACHES TO
... this research. Then, the domain complexity of proteins was analysed on a comprehensive basis. Our results showed that bacterial and archaeal proteins are as complex as eukaryotic proteins in domain abundance, but more promiscuous. Proteins emerged in early stage are also more promiscuous, but with l ...
... this research. Then, the domain complexity of proteins was analysed on a comprehensive basis. Our results showed that bacterial and archaeal proteins are as complex as eukaryotic proteins in domain abundance, but more promiscuous. Proteins emerged in early stage are also more promiscuous, but with l ...
Neutrophils in the innate immune response
... human innate immune system. Circulating neutrophils are rapidly recruited to sites of infection by host- and/or pathogen-derived components, which also prime these host cells for enhanced microbicidal activity. PMNs bind and ingest microorganisms by a process known as phagocytosis, which typically t ...
... human innate immune system. Circulating neutrophils are rapidly recruited to sites of infection by host- and/or pathogen-derived components, which also prime these host cells for enhanced microbicidal activity. PMNs bind and ingest microorganisms by a process known as phagocytosis, which typically t ...
Hormones and Signal Transduction III
... RAS RAS is a Family of Related Proteins Each is Monomeric and like the α-subunit of G-Proteins RAS Proteins Bind Guanine Nucleotides RAS Swaps GDP for GTP on Activation RAS Slowly Cleaves GTP to GDP ...
... RAS RAS is a Family of Related Proteins Each is Monomeric and like the α-subunit of G-Proteins RAS Proteins Bind Guanine Nucleotides RAS Swaps GDP for GTP on Activation RAS Slowly Cleaves GTP to GDP ...
Modulation of the Antigenic Peptide Transporter TAP by
... been characterized in detail. ICP47 of herpes simplex virus 1 (HSV-1) acts as a high-affinity inhibitor in the cytosol by blocking peptide binding to TAP.15,16 By contrast, US6 of human cytomegalovirus (HCMV) binds to TAP in the ER and inhibits peptide translocation by blocking ATP but not peptide b ...
... been characterized in detail. ICP47 of herpes simplex virus 1 (HSV-1) acts as a high-affinity inhibitor in the cytosol by blocking peptide binding to TAP.15,16 By contrast, US6 of human cytomegalovirus (HCMV) binds to TAP in the ER and inhibits peptide translocation by blocking ATP but not peptide b ...
Apoptosome
The apoptosome is a large quaternary protein structure formed in the process of apoptosis. Its formation is triggered by the release of cytochrome c from the mitochondria in response to an internal (intrinsic) or external (extrinsic) cell death stimulus. Stimuli can vary from DNA damage and viral infection to developmental cues such as those leading to the degradation of a tadpole's tail.In mammalian cells, once cytochrome c is released, it binds to the cytosolic protein Apaf-1 to facilitate the formation of apoptosome. An early biochemical study suggests a two-to-one ratio of cytochrome c to apaf-1 for apoptosome formation. However, recent structural studies suggest the cytochrome c to apaf-1 ratio is one-to-one. It has also been shown that the nucleotide dATP as third component binds to apaf-1, however its exact role is still debated. The mammalian apoptosome had never been crystallized, but a human APAF-1/cytochrome-c apoptosome has been imaged at lower (2 nm) resolution by cryogenic transmission electron microscopy 10 years ago, revealing a wheel-like particle with 7-fold symmetry. Recently, a medium resolution (9.5 Ångström) structure of human apoptosome was also solved by cryo-electron microscopy, which allows unambiguous inference for positions of all the APAF-1 domains (CARD, NBARC and WD40) and cytochrome c. There is also now a crystal structure of the monomeric, inactive Apaf-1 subunit (PDB 3SFZ). Once formed, the apoptosome can then recruit and activate the inactive pro-caspase-9. Once activated, this initiator caspase can then activate effector caspases and trigger a cascade of events leading to apoptosis.