Chapter 13
... 15. Describe stages of bacterial growth: log, lag (exponential growth), stationary, death phases 16. Define: prototroph, auxotroph, minimal, selective, and complete media 17. Determine bacterial titer (colony forming units/ml) 18. Contrast nutritional, conditional, and resistance mutations in bacter ...
... 15. Describe stages of bacterial growth: log, lag (exponential growth), stationary, death phases 16. Define: prototroph, auxotroph, minimal, selective, and complete media 17. Determine bacterial titer (colony forming units/ml) 18. Contrast nutritional, conditional, and resistance mutations in bacter ...
Ligation and Transformation
... Process 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
... Process 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
Genetic Control of Metabolism
... • New strains are also produced by bacteria taking up DNA fragments from their environment. • Scientists try to produce new strains of useful bacteria by culturing existing strains together in conditions where horizontal transfer of DNA is most likely to occur. ...
... • New strains are also produced by bacteria taking up DNA fragments from their environment. • Scientists try to produce new strains of useful bacteria by culturing existing strains together in conditions where horizontal transfer of DNA is most likely to occur. ...
Recombinant Paper Plasmids:
... You have now prepared a pAMP plasmid and a pKAN plasmid. In this pare of the activity, you will use them as starting materials to make a recombinant plasmid. You will cut pAMP and pKAN with two specific enzymes, BamHI and HindIII. You will ligate together fragments that come from each plasmid, creat ...
... You have now prepared a pAMP plasmid and a pKAN plasmid. In this pare of the activity, you will use them as starting materials to make a recombinant plasmid. You will cut pAMP and pKAN with two specific enzymes, BamHI and HindIII. You will ligate together fragments that come from each plasmid, creat ...
IS91 transposase is related to the rolling-circle
... are 35% identical proteins of 426 and 410 amino acids respectively (2,3). Apart from this, IS91 is unrelated to other presently known IS elements. Figure 1 shows the four conserved motifs between the IS91/IS8O1 transposases and a family of five replication proteins of plasmids pUBHO, pLABlOOO, pLPl, ...
... are 35% identical proteins of 426 and 410 amino acids respectively (2,3). Apart from this, IS91 is unrelated to other presently known IS elements. Figure 1 shows the four conserved motifs between the IS91/IS8O1 transposases and a family of five replication proteins of plasmids pUBHO, pLABlOOO, pLPl, ...
Molecular Analysis of Lactic Acid Bacteria in an Inhospitable
... 16S rRNA gene sequences from these isolates grouped them phylogenetically with the clades from the sediment DNA (FIG 1). The “flat” colony type was identified by BLAST analysis as Lactobacillus brevis, the most common beer spoilage isolate. The 16S rRNA gene sequence of the “tight” isolate, however, ...
... 16S rRNA gene sequences from these isolates grouped them phylogenetically with the clades from the sediment DNA (FIG 1). The “flat” colony type was identified by BLAST analysis as Lactobacillus brevis, the most common beer spoilage isolate. The 16S rRNA gene sequence of the “tight” isolate, however, ...
plasmids - genemol de Jean
... rare that induced transformation is more important to the geneticist. A captured DNA has to face restriction! ...
... rare that induced transformation is more important to the geneticist. A captured DNA has to face restriction! ...
Gene Technology - Manasquan Public Schools
... Restriction Enzymes/Restriction Endonucleases (DNA scissors) cut at known sequences in specific places (pallindromes) Ligase Cloning vector ...
... Restriction Enzymes/Restriction Endonucleases (DNA scissors) cut at known sequences in specific places (pallindromes) Ligase Cloning vector ...
C13 Genetic Engineering
... Cutting DNA into pieces is done with restriction enzymes. Each one cuts DNA at a specific sequence of nucleotides. Separating DNA can be achieved by using gel electrophoresis. In DNA electrophoresis, the DNA cut with restriction enzymes is put into the well at one end (negative end – black) of the g ...
... Cutting DNA into pieces is done with restriction enzymes. Each one cuts DNA at a specific sequence of nucleotides. Separating DNA can be achieved by using gel electrophoresis. In DNA electrophoresis, the DNA cut with restriction enzymes is put into the well at one end (negative end – black) of the g ...
Ch. 13 SOL - Groupfusion.net
... human cells able to resist antibiotics human cells unable to synthesize antibodies bacterial cells able to synthesize human insulin bacterial cells unable to synthesize human insulin ...
... human cells able to resist antibiotics human cells unable to synthesize antibodies bacterial cells able to synthesize human insulin bacterial cells unable to synthesize human insulin ...
Structural analysis of the protein complex involved in the
... its molecular mechanisms. Some eubacteria possess plasmid DNA that has acquired various antibiotic-resistant genes. The plasmid DNA is maintained in the bacteria by two types of protein, known as toxin and antitoxin. Researchers have crystallized the free YoeB toxin and the YoeB (toxin)-YefM (antito ...
... its molecular mechanisms. Some eubacteria possess plasmid DNA that has acquired various antibiotic-resistant genes. The plasmid DNA is maintained in the bacteria by two types of protein, known as toxin and antitoxin. Researchers have crystallized the free YoeB toxin and the YoeB (toxin)-YefM (antito ...
Mutations
... – expansion of ecological niche, increased virulence – occurs in the three mechanisms evolved by bacteria to create recombinants – genes can be transferred to the same or different species ...
... – expansion of ecological niche, increased virulence – occurs in the three mechanisms evolved by bacteria to create recombinants – genes can be transferred to the same or different species ...
Lab 6 DNA ISOLN
... cells are exposed to NaOH and a strong detergent (alkaline lysis) to denature de Chromosomal DNA (i.e. the 2 strands are separated). An acidic solution of sodium acetate is then added to neutralize the solution. At this point, most of the cell membrane material and the genomic DNA are spilled out an ...
... cells are exposed to NaOH and a strong detergent (alkaline lysis) to denature de Chromosomal DNA (i.e. the 2 strands are separated). An acidic solution of sodium acetate is then added to neutralize the solution. At this point, most of the cell membrane material and the genomic DNA are spilled out an ...
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
... genotype different from either parent (exchange of DNA between different genes) 1. occurs during meiosis as crossing over between homologous chromosomes 2. genetic recombination (homologous recombination) is the most common form, involving reciprocal exchange between a pair of homologous DNA sequenc ...
... genotype different from either parent (exchange of DNA between different genes) 1. occurs during meiosis as crossing over between homologous chromosomes 2. genetic recombination (homologous recombination) is the most common form, involving reciprocal exchange between a pair of homologous DNA sequenc ...
Supplementary METHODS
... containing psoralen ICLs, UVC-induced damage, or no damage was subjected to an in vitro repair assay as described in the Methods section. Then the plasmids were digested with EcoRI and SacI to release the 188 bp fragment surrounding the site-specific ICL. Visualization of the plasmid DNA and the inc ...
... containing psoralen ICLs, UVC-induced damage, or no damage was subjected to an in vitro repair assay as described in the Methods section. Then the plasmids were digested with EcoRI and SacI to release the 188 bp fragment surrounding the site-specific ICL. Visualization of the plasmid DNA and the inc ...
pGlo Power Point Presentation
... Originally evolved by bacteria May express antibiotic resistance gene or be modified to express proteins of interest ...
... Originally evolved by bacteria May express antibiotic resistance gene or be modified to express proteins of interest ...
: Determining DNA sequences
... sites : vary in size from sites of 4bp to 8bp or longer; 4 bp cuts into fragments of 256 bp in size ; of 8 b.p 4 8 (64,000) b.p. ; e.g. EcoR1 site: GAATTC – Restriction maps: map of restriction enzyme sites (refer to figure 17.5 klug) ...
... sites : vary in size from sites of 4bp to 8bp or longer; 4 bp cuts into fragments of 256 bp in size ; of 8 b.p 4 8 (64,000) b.p. ; e.g. EcoR1 site: GAATTC – Restriction maps: map of restriction enzyme sites (refer to figure 17.5 klug) ...
No Slide Title
... B. Unique restriction site Multiple cloning site ( polylinker) FIGURE 20.3 C. Origin of replication ...
... B. Unique restriction site Multiple cloning site ( polylinker) FIGURE 20.3 C. Origin of replication ...
MS Word file
... Merozygotes: partial diploid bacterial cells containing F plasmid carrying some bacterial genes Mapping bacterial genes with interrupted Conjugation: Distances between genes are measured by the time required for DNA transfer from Hfr cells to F− cells. ...
... Merozygotes: partial diploid bacterial cells containing F plasmid carrying some bacterial genes Mapping bacterial genes with interrupted Conjugation: Distances between genes are measured by the time required for DNA transfer from Hfr cells to F− cells. ...
4.4 Genetic engineering and biotechnology – summary of mark
... Describe the application of DNA profiling to determine paternity and also in forensic investigations. Mark Scheme A. sample of DNA / blood / saliva / semen is obtained; B. satellite DNA / repetitive sequences used for profiling; C. reference samples of DNA are obtained; D. PCR used to amplify / prod ...
... Describe the application of DNA profiling to determine paternity and also in forensic investigations. Mark Scheme A. sample of DNA / blood / saliva / semen is obtained; B. satellite DNA / repetitive sequences used for profiling; C. reference samples of DNA are obtained; D. PCR used to amplify / prod ...
See Fig. 13.1c
... Special metabolic properties - some plasmids allow bacteria to take advantage of situations that might be otherwise harmful. Breakdown of complex organic molecules is often plasmid encoded ...
... Special metabolic properties - some plasmids allow bacteria to take advantage of situations that might be otherwise harmful. Breakdown of complex organic molecules is often plasmid encoded ...
Lab 4
... The purpose of this lab is to introduce you to methods for cloning your PCR products amplified using Taq polymerase. Detailed information is available in Invitrogen’s TOPO TA cloning manual, available on their website or our course webpage. Bacteria (such as E. coli that we are using in today’s lab) ...
... The purpose of this lab is to introduce you to methods for cloning your PCR products amplified using Taq polymerase. Detailed information is available in Invitrogen’s TOPO TA cloning manual, available on their website or our course webpage. Bacteria (such as E. coli that we are using in today’s lab) ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.